Preparation of N2, N2,7-trimethylguanosine affinity columns

2,2,7-trimethylguanosine (TMG) binding proteins from human cells were purified through TMG-affinity columns. TMG synthesis was improved and the TMG obtained was shown to be similar to the TMG in the 5' cap of the UsnRNAs. The eluates obtained with TMG-affinity chromatographies were very different from those isolated with m7G-affinity columns, thus suggesting that specific TMG- binding proteins were obtained. The fraction may be enriched with factors associated with import and/or hypermethylation of UsnRNPs.

Chitosan nanoparticles for non-viral gene therapy

The cationic polysaccharide chitosan has been widely used for non-viral transfection in vitro and in vivo and has many advantages over other polycations. Chitosan is biocompatible and biodegradable and protects DNA against DNase degradation. However following administration the ChitosanDNA polyplexes must overcome a series of barriers before DNA is delivered to the cell nucleus. This paper describes the most important parameters involved in the chitosan-DNA interaction and their effects of on the condensation, shape, size and protection of DNA. Strategies developed for chitosanDNA polyplexes to avoid non-specific interaction with blood components and to overcome intracellular obstacles as the crossing of die cell membrane, endosomal escape and nuclear import are presented. © 2006 American Chemical Society.

Preliminary Overview of the Economies of Latin America and the Caribbean 2004

Includes bibliography

Economic Survey of Latin America and the Caribbean 2010-2011: Internacional integration and macroeconomic policy challenges amid global economic turmoil

Includes bibliography

Export growth in Latin America: policies and performance

Includes bibliography

Latin America and the Caribbean: policies to improve linkages with the global economy

Includes bibliography

Structural change and productivity growth, 20 years later: old problems, new opportunities

Includes bibliography

Barriers to Latin American and Caribbean exports in the US market, 1998-1999

Includes bibliography

Biofuels potential in Guyana

Includes bibliography

The contribution of biofuels to the sustainability of development in Latin America and the Caribbean

Includes bibliography

Caribbean trade and integration: trends and future prospects

Includes Bibliography

Antidumping in the Americas

Includes bibliography

Productivity growth in Latin American manufacturing: what role for international trade intensities?

Includes Bibliography

Latin America's experience in dispute settlement within WTO: the cases of technical standards, sanitary and phytosanitary measures and intellectual property

Includes Bibliography

E-commerce and export promotion policies for small- and medium-sized enterprises: East Asian and Latin American experiences

Includes bibliography