941 resultados para Diversification
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Esta pesquisa se insere dentro da história da agricultura no Brasil, especialmente no Estado de São Paulo, a partir da segunda metade do século XIX, início do século XX. As propostas de modernização agrícola giravam em torno de diferentes assuntos, mas sempre interligados, como: diversificação da agricultura, adubação química e natural, cultura intensiva do solo, mão de obra, povoamento e colonização, instalação de núcleos coloniais dentro dos moldes da moderna agricultura então desejada, instrução agrícola por meio de campos de experiências e demonstração, ensino agrícola para diferentes graus, a mecanização da lavoura, o que era chamado, na época, de Moderna Agricultura, em substituição ao que era considerado como agricultura rotineira ou atrasada, praticada desde o início da agricultura no Brasil.
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Chromosome mapping and studies of the genomic organization of repetitive DNA sequences provide valuable insights that enhance our evolutionary and structural understanding of these sequences, as well as identifying chromosomal rearrangements and sex determination. This study investigated the occurrence and organization of repetitive DNA sequences in Leporinus elongatus using restriction enzyme digestion and the mapping of sequences by chromosomal fluorescence in situ hybridization (FISH). A 378-bp fragment with a 54.2% GC content was isolated after digestion with the SmaI restriction enzyme. BLASTN search found no similarity with previously described sequences, so this repetitive sequence was named LeSmaI. FISH experiments were conducted using L. elongatus and other Anostomidae species, i.e. L. macrocephalus,L. obtusidens, L. striatus, L. lacustris, L. friderici, Schizodon borellii, S. isognathus, and Abramites hypselonotus which detected signals that were unique to male and female L. elongatus individuals. Double-FISH using LeSmaI and 18S rDNA showed that LeSmaI was located in a nucleolus organizer region (NOR) in the male and female metaphases of L. elongatus. This report also discusses the role of repetitive DNA associated with NORs in the diversification of Anostomidae species karyotypes. Copyright © 2012 S. Karger AG, Basel.
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