Cartas e documentos dirigidos a Sua Magestade o Senhor D. João VI pelo Principe Real o Senhor D. Pedro de Alcântara : e que forão presentes às Cortes em a Sessão de 28 de septembro de 1822

A carta na qual Dom Pedro comunica a Independência do Brasil é datada de 26 de setembro de 1822 e contém a ata da sua aclamação como Imperador Constitucional e Defensor Perpétuo do Brasil.

Cartas, e mais peças officiaes, dirigidas a Sua Magestade, o Senhor D. João VI pelo Principe Real, o Senhor D. Pedro de Alcantara em data de 26, e 28 de abril deste anno

D. Pedro relata a sua viagem a Minas Gerais e a decisão de convocar as Cortes Gerais do Brasil. Diz : "V. M...., quando se ausentou deste rico e fértil país, recomendou-me que tratasse os brasileiros como filhos. Eu não só os trato como taes, mas também como amigos".

In the footstep of Hermes: the meaning of the hermeneutics and symbolism

Translated by Michael Marder

Espírito de Vieira, ou, selecta de pensamentos econômicos, políticos, moraes, literários : com a biographia deste celebrado escriptor / por José da Silva Lisboa

A bibliografia da Impressão Régia do Rio de Janeiro diz: "Terminada a publicação dos EStudos do bem comum... (1819-1820), José da Silva Lisboa planejou publicar uma coletânea dos esccritos do Padre Vieira como apêndice à obra..."

Tratado do jogo do voltarete : com as leis geraes do jogo

Os dados de autoria foram retirados da fonte de referência.

Fallas do throno, desde o anno de 1823 ate o anno de 1872, acompanhadas dos respectivos votos de graças da Camara Temporaria e de differentes informações e esclarecimentos / colligidas na Secretaria da Camara dos Srs. Deputados

Contém uma relação de todos os deputados brasileiros desde as cortes portuguesas e a constituinte até a 14ª legislatura ordinária.

Online exercise for the design and simulation of PCR and PCR-RFLP experiments

[EN] Background: Polymerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphism of PCR products (PCR-RFLP) are extensively used molecular biology techniques. An exercise for the design and simulation of PCR and PCR-RFLP experiments will be a useful educational tool. Findings: An online PCR and PCR-RFLP exercise has been create that requires users to find the target genes,compare them, design primers, search for restriction endonucleases, and finally to simulate the experiment. Each user of the service is randomly assigned a gene from Escherichia coli; to complete the exercise, users must design an experiment capable of distinguishing among E. coli strains. By applying the experimental procedure to all completely sequenced E. coli, a basic understanding of strain comparison and clustering can also be acquired. Comparison of results obtained in different experiments is also very instructive. Conclusions: The exercise is freely available at http://insilico.ehu.es/edu.

Genetics of Type III Bartter Syndrome in Spain, Proposed Diagnostic Algorithm

9 p.

Aplicación de técnicas estadísticas en el estudio de fenómenos ambientales y ecosistémicos

208 p.

Inoculation of Triatoma Virus (Dicistroviridae: Cripavirus) elicits a non-infective immune response in mice

Background: Dicistroviridae is a new family of small, non-enveloped, +ssRNA viruses pathogenic to both beneficial arthropods and insect pests. Little is known about the dicistrovirus replication mechanism or gene function, and any knowledge on these subjects comes mainly from comparisons with mammalian viruses from the Picornaviridae family. Due to its peculiar genome organization and characteristics of the per os viral transmission route, dicistroviruses make good candidates for use as biopesticides. Triatoma virus (TrV) is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of the human trypanosomiasis disease called Chagas disease. TrV was postulated as a potential control agent against Chagas' vectors. Although there is no evidence that TrV nor other dicistroviruses replicate in species outside the Insecta class, the innocuousness of these viruses in humans and animals needs to be ascertained. Methods: In this study, RT-PCR and ELISA were used to detect the infectivity of this virus in Mus musculus BALB/c mice. Results: In this study we have observed that there is no significant difference in the ratio IgG2a/IgG1 in sera from animals inoculated with TrV when compared with non-inoculated animals or mice inoculated only with non-infective TrV protein capsids. Conclusions: We conclude that, under our experimental conditions, TrV is unable to replicate inmice. This study constitutes the first test to evaluate the infectivity of a dicistrovirus in a vertebrate animal model.

推动交叉学科研究 促进生命与医学科学的发展

<正>基础研究是国家科技发展的原动力,学科的交融与渗透已经成为当今科学发展的重要趋势,促进交叉学科的健康成长是目前科学界普遍关心的问题之一。分析近百年来获得诺贝尔自然科学奖的300多项成果中,近一半的项目是多学科合作的研究成果,对170多位生理学或医学诺贝尔奖获得者及他们的原创性成果的统计研究发现,具有跨学科知识

双模式高精度可调超分辨光瞳滤波器

基于双折射波片的特性，提出了一种具有双调节模式的连续可调超分辨光瞳滤波器。该滤波器通过两种调节模式实现超分辨，且在一定范围内连续可调。在横向旋转模式下，该滤波器为复振幅型光瞳滤波器，纵向倾斜模式下为纯相位型超分辨滤波器。通过对超分辨参量的分析，得到最佳设计参量，可以实现大的视场角，大的超分辨范围、高的调节精度。这种滤波器为实际应用提供了更多的方便。