Efeito do treinamento resistido na densidade mineral óssea e no conteúdo de GLUT4 em ratos diabéticos (tipo 2) osteopênicos

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Differential environmental impacts on small and medium size rivers from center of São Paulo State, Brazil, and regional management perspectives

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Influência do herbicida Glifosato (N-Fosfonometil)Glicina) na formação de biomassa da alga Pseudokirchneriella subcapitata (Chlorophyceae)

Herbicides are chemical agents most consumed for agricultural production and of these, glyphosate (N-(phosphonomethyl) glycine) represents more than half of world consumption of non-selective herbicides, use of systemic action and post-emergent. The present study aims to analyze the interference of different concentrations of glyphosate in the development of micro algae, unicellular species Pseudokirchneriella subcapitata, with supplementary analysis of the interference of green algae concentrations in the artificial lake Instituto de Biociências Universidade Estadual Paulista in Rio Claro, SP. To identify the effect of glyphosate on the development of microalgae concentrations: 0.118; 0.236; 0.472; 0.944; 1.888 and 3.776 mg of product (all in rejoinders), maintaining control without glyphosate, were inoculated into flasks containing 150mL of medium culture and 5mL of suspension inoculum levels. Each test was continued for 96 hours in a shaker rotating at 150 rpm at 25 / - 2 ° C and constant light of 3200 lux. At baseline and 48; 72 and 96 hours samples were taken for testing for absorbance. The final dry weight was measured and the tests with P. subcapitata cell number was quantified at the beginning and end of the tests using the Neubauer chamber under optical microscope. For algae of the artificial lake of the diversity of microalgae cells was identified through photographic documentation. The analysis of variance comparing treatments for dry biomass showed no significant differences in the tests with P. subicapitata as for the algae to the lake.The analysis of variance MANOVA showed significant differences between the treatments over time but there was no significant difference between the interactions of treatment for tests with P. subicapitata There were significant differences in the tests with microalgae of the artificial lake. With respect to cell quantification, it was found... (Complete abstract click electronic acces below)

Estudo da qualidade da água distribuída pelo poço artesiano da FEG em relação à água servida pelo abastecimento público de Guaratinguetá

We conducted a study of the quality of water distributed by Artesian Well in relation to water supply served by the public for purposes of human consumption of UNESP Campus Guaratinguetá. Two sections of the network were randomly chosen and monitored for 105 days: the stretch of the pipeline started off the Water Treatment Station of the Autonomous Service of Water, Sewage and waste in the Municipality of Guaratinguetá and ended in a point consumption of the Child Family Center, while the section B began at the output of an artesian well and finished in a point of consumption of the Department of Civil Engineering. It is the deterioration of water quality in parts A and B, through comparative analysis of Bicarbonates, Total Hardness, Total Iron, free residual chlorine, pH, Total Residual, Turbidity, Conductivity, Color, Fecal Coliforms and Total Coliforms. Among the observations that can be highlighted: the Total Hardness was found in highest concentration in the extract; for Total Iron significant variations were obtained for both parts, and reduction of this parameter in two points of consumption analyzed, not the expected increase concentration, because the contact of water along the pipe, the CRL showed significant depletion to section A, which can be related to impurities in the water, the presence of biofilm on the walls of pipes, or because this is the stretch of greater extent to be covered by water for pH, this indicated that the water is alkaline and slightly acidic stretch in section B; Turbidity was found for the higher concentration to the passage, and a considerable reduction of this parameter at point consumption due to deposition of particulate matter along the stretch, the conductivity also showed a higher concentration in the extract, as well as an increase in this parameter at the point of consumption. Upon leaving ... (Complete abstract click electronic access below)

Desenvolvimento de um computador de bordo para veículo Baja-SAE

The objective of this paper is show the development of an Eletronic Control Unit (ECU) for Baja-SAE vehicles from its requirements to final implementation. An ECU for Baja-SAE vehicles is made of electric and electronic circuits (appropriated to the hostile situations of an off-road competition) able to make important measurements for the running or stand-by vehicle performance. For this development, some concepts were studied: embedded electronics concepts (mainly microcontrollers and data acquisition circuits) and sensoring. Both the studies were made considering the off-road environment. After these initial studies, the elaboration of the basics hardware and software (under simulations) has begun, and this elaboration objectives maximum performance and low implementation cost. After the theorical studies, basic definition of the hardware and software’s architectures and simulations, the printed circuit boards (PCB) were made, as exhaustive tests in these boards for debugging and verify if the boards work well. Once the system was tested in the university, it ran into a regional Southeast competition in 2010, that definitely proved the efficiency of the whole system

Análise de fácies sedimentares e de paleocorrentes das sequências inferiores do grupo Itararé, Centro-Leste do estado de São Paulo

The Itararé Group is the record of one of the most significant glaciation events of the Gondwana. Of neocarboniferous-eopermian age, this unit has facies associations interpreted as resulted from shallow marine settings with glacial and deglacial water influence. The role of glaciers on the tectonic-sedimentary processes of the Itararé Group is striking, as typical facies such as diamictites, tilites, shales and rhythmites with dropstones are generated, besides the existence of glacially striated and polished surfaces. This work had as main goal to contribute to the geological knowledge of the Itararé Group in the central eastern portion of São Paulo State, using as basis outcrops from Itu, Salto and Porto Feliz, and also pertinent bibliographic data and well profiles located in the surrounding areas. About the stratigraphic framework, genetic association and paleogeography of these deposits, this work concludes that the area studied comprises the basal section of the Itararé Group. The stacking of the facies recognized during the field work begins with the striated and polished surfaces of the basement, described in Salto, superposed by tilites and diamictites with glacial movement features. Over these are the turbidites from Itu, associated with the glacier retreat. The outcrops of Porto Feliz are at the top of this succession, with deposits generated by flows with lateral and vertical variations, sometimes with prevalence of tractive conditions, and sometimes with sediments transported largely by suspension. Paleocurrent measurements show main flow directions towards NW, the same as glacial striae, suggesting the influence of reworking of sediments by the meltwater during deglacial periods

Avaliação da atividade antidepressiva do óleo essencial de Cananga odorata (ylang-ylang) em camundongos, por via oral

Sabe-se que o número de plantas com atividade comprovada no sistema nervoso central vem crescendo ultimamente, resultado do interesse renovado nesta linha de investigação. O óleo essencial da espécie Cananga odorata (Lam.) Hook. f. & Thomson possui indícios de uma potencial atividade do tipo antidepressiva. A depressão maior ou depressão unipolar é um distúrbio do sistema nervoso central com potencial para se tornar um dos maiores problemas de saúde em termos de qualidade de vida e incapacitação da população mundial em alguns anos, e os tratamentos existentes na clínica possuem diversos problemas, principalmente relacionados a efeitos colaterais. O objetivo deste projeto foi avaliar a atividade antidepressiva do óleo essencial da espécie Cananga odorata (ylang-ylang), administrado por via oral em camundongos. Foram utilizados camundongos Swiss, machos, com aproximadamente 45 dias de idade. O óleo essencial foi solubilizado em Tween, nas seguintes concentrações: 1, 10 e 100 mg/kg (administrado pela via oral – v.o.). O grupo controle negativo recebeu apenas Tween (v.o.), e o grupo controle positivo recebeu imipramina 30 mg/kg (via intraperitoneal), sempre no volume de 10 mL/kg. Os animais foram submetidos ao Teste da Suspensão pela Cauda, para avaliação da atividade do tipo antidepressiva, medindo-se o tempo total de imobilidade e a latência para o aparecimento do primeiro comportamento de imobilidade (em segundos); e ao Teste do Campo Aberto, para avaliação de atividade motora, medindo-se o número de quadrantes percorridos (indicador de ambulação espontânea), e o número de rearings (indicador de comportamento exploratório). Não foi observado efeito do tipo antidepressivo ou sobre a atividade motora e motivacional, em nenhuma das doses testadas do óleo essencial de ylang-ylang, provavelmente pela dificuldade na correlação entre a concentração administrada pela via inalatória e a dose por via oral

Efeitos do deplecionamento induzido do reservatório de Salto Grande, Rio Paranapanema(SP/PR) sobre as assembléias de microcrustáceos (Cladocera e Copepoda) de duas lagoas marginais

No reservatório de Salto Grande (22º53’53’’S / 49º59’33’’W), Rio Paranapanema (SP/PR), é realizado um tipo de manejo (redução do nível de água) para redução da biomassa da macrófita submersa Egeria densa desde 2004. Estas plantas causam perdas econômicas para o programa de geração elétrica e infortúnio para a população local. O reservatório de Salto Grande possui lagoas marginais que apresentam elevada densidade dessas macrófitas e são tidas como ambientes de alta importância para o desenvolvimento, alimentação e reprodução de muitas assembléias, principalmente as de microcrustáceos zooplanctônicos. O objetivo deste trabalho foi analisar as mudanças na estrutura (composição, riqueza, abundância e diversidade) das assembléias de microcrustáceos zooplanctônicos (Cladocera e Copepoda) e suas relações com as alterações nas condições limnológicas decorrentes da variação de nível em duas lagoas marginais (Pedra Branca e Guaritá) em função do deplecionamento. Foram realizadas amostragens mensais de abril/2005 a julho/2006, além de coletas nictemerais durante o processo de deplecionamento (agosto/05) e um mês após o mesmo. Para a análise do zooplâncton foram amostrados 150 litros de água superficial em cada lagoa (região limnética). Simultaneamente, foram medidas as variáveis físicas e químicas da água: temperatura, pH, oxigênio dissolvido, condutividade elétrica, transparência, turbidez, material em suspensão (total, frações orgânica e inorgânica) e concentração de clorofila a. As variáveis limnológicas demonstraram um padrão de variação sazonal, além da influência do deplecionamento, principalmente sobre a temperatura e oxigênio dissolvido. O valor de riqueza encontrado para Cladocera foi elevado, totalizando 49 táxons. Os Copepoda foram analisados apenas até o nível de Ordem... (Resumo completo, clicar acesso eletrônico abaixo)

Produção de hidrolisado enzimático de farinha de batata-doce para fermentação etanólica

Recently there is a great quest of producing alcohol from starchy resources, replacing the sugar cane. The most common starchy sources are cassava, maize and sweet potatoes and a lot of research are been realized with excellent results. In this work it was evaluated the influence of the concentration of dry matter on the enzymatic hydrolysis process of starch from sweet potato for ethanol production. Through the sweet potato was produced a flour using a low-cost method and easy operation equipments. The sweet potato flour was characterized physical and chemically and from these results was prepared the treatments for enzymatic hydrolysis. The experimental design considered as independent variable the dry matter concentration of the sweet potato flour in 3 levels; 10, 15 and 20% in the formulation of suspensions. The other variables were keeping constant being: temperature in the 1° hydrolysis step of 90°C and time of 2 hours; temperature in the 2° saccharification step of 60°C and time of 17 hours. The hydrolysates obtained at the three assays were transferred to six liter enlerynmeyer and inoculated with a biologic catalyst, Saccharomyces, dehydrated yeasts of Saccharomyces cerevisiae CAT 1, at a rate of 5% in weight. The flasks were placed in a shaker type orbital with controlled temperature of 30°C during a time of 15 hours. The initial reducer sugars concentration and respective ethanol concentrations in wine were: 11.2% glucose and 2.16% ethanol in the suspension with 10% of dry matter; 13.5% glucose and 4.39% ethanol with 15% and 17.5% glucose and 6.03% ethanol in suspension with 20% of dry matter. ix The results showed that the higher percentage of dry matter carried out to higher sugar yield in hydrolyzed. It was possible observed that products quality improved with a higher concentration of dry matter

Aplicabilidade das equações isotermas de Freundlich e Langmuir na interação biossortiva de corantes têxteis com Saccharomyces cerevisiae liofilizada

This paper presents a study of the applicability of adsorption isotherms, known as Langmuir and Freundlich isotherm, between the biosorptive interaction of yeast lyophilized Saccharomyces cerevisiae and textile dyes. To that end, we prepared stock solutions of the textile dyes Direct Red 23 and Direct Red 75 in the concentration of 1.000μg/mL and a yeast suspension at 2,5%. We did experiments for two cases, firstly for the case that we have a fix concentration of yeast at 0,500mg/mL and an variable concentration of dye range from40, 50, 60, 80 and 100μg/mL, then for the case that we fixed the concentration of dye at 100μg/mL and the yeast concentration was variable range from 0,250, 0,500, 0,750, 1,000, 1,250mg/mL. For the dye Direct Red 23 we did analysis in the pH 2,5, 4,5 and 6,5; for the Direct Red 75, we just did for the pH 2,5. We leave the dye solution in contact with the yeast for 2 hours at a constant temperature of 30°C and then centrifuged and analyzed the sample in a spectrophotometer and finally made and analysis of parameters for the removal and study of the isotherms. After the biosorption, was observed that for the Direct Red 23 in the pH 2,5 was needed 1,407mg/mL of yeast for total removal, while for the pH 4,5 was needed 8,806mg/mL and in pH 6,5 was 9,286mg/mL; for the Direct Red 75 in pH 2,5 was needed 1,337mg/mL. This difference can be explain by the adsorption isotherms, was observed that in the case when the yeast was fix when we had in a acid pH the behavior of the system was compatible with the Langmuir isotherm, and thus, an monolayer pattern. And that when we decrease the acidity of the medium the system became more compatible with a Freundlich isotherm, and thus, a multilayer pattern; for the case that the yeast was variable this is not much evident, however for the pH 2,5 she became compatible with a Langmuir isotherm... (Complete abstract click electronic access below)

Biodegradação da vinhaça resíduo da produção de etanol

The State of São Paulo is responsible for the largest sugar cane production in Brazil, as well as the largest production of ethanol made of this raw material – which is widely used as fuel for automobiles. This utilization began in the 1970’s, with the institution by the Brazilian government of the National Alcohol Program (PRO-ÁLCOOL), as a consequence of the petroleum crisis, rising again five years ago, with the development of flex fuel cars. The obtaining process of ethanol originates residues; amongst them, vinasse is the one that’s generated in the largest amount (an average of 10 to 13 litres/litre of ethanol produced). The disposal of this residue in waters was only forbidden in 1978, but before that, researchers had already been investigating its utilization as raw material. This paper had the objective of accompany the biodegradation of vinasse by evaluating the oxygen comsumption during it until the ultimate Biochemical Oxygen Demand (uBOD), performed in twenty days; another objective was to analyse the biomass production of Saccharomyces cerevisae in this residue. Physical and chemical analyses of the residue were also performed, as well as acute toxicity essays using Daphnia similis and Dugesia tigrina, before and after its biodegradation. The physical and chemical analyses pointed elevated acidness (pH = 3,98), conductivity (8,30 mS/cm) and COD (25.693,43 mg O2/L) and mean quantity of suspended solids (5.246 mg/L). The toxicity essays indicated absence of toxic potential in vinasse after biodegradation for both species. The uBOD degradated until 88,22% of the COD, demonstrating the possibility of biodegradation of most of the residue’s organic load in a relatively short period of time. S. cerevisae caused a 37,03% COD diminution in vinasse, diminished its conductivity and promoted a slight elevation of the pH; it obtained low biomass...(Complete abstract click electronic access below)

Redução de empeno em barra trefiladas do aço SAE 9254

In this paper we study the process of manufacture of wire drawn steel bars 9254, from rolled wire rod. These bars are used in the automotive industry for the manufacture of coil springs, which make up the system damping of several vehicles. The wire drawing process consists of the steps of pre-straightening, shot peening, drawing, cutting and polishing. The study aims to search for the configuration of process variables, which present the best result with respect to bending. To this were maintained settings prestraightening and mechanical stripping and varied angles and stringer polish being studied to replace the spinneret with a working angle, for a string with two working angles. To assist in the analysis of the results was the tool used DOE Software Minitab, which assesses the variation in results according to each parameter and the interaction parameters. It was thus possible to determine the best condition for wire drawing

Otimização da estrutura metalica para um carro alegórico

Carnival is a unique opportunity for the associations demonstrate the community’s annual work and the float is a major component of this celebration, responsible for pitch the samba using allegories and carry important figures. The excess weight of the floats which are mostly manufactured from truck and bus structures requires larger amount of people to push the vehicle in addition to increased efforts on the steering systems, suspension and wheels, raising the probability of breakage and loss of one year work. The objective of this study is to use the concepts of strength of materials combined with computer simulation to obtain a structure that has the lowest possible weight without compromising the safety of transported components and that is also easy to manufacture, drive and store

Freqüência do parvovírus suíno em fetos mumificados, abortados e natimortos detectado através da reação em cadeia pela polimerase (PCR)

Porcine parvovirus (PPV) is associated with reproductive failure and it has been found worldwide, including Brazil. Many diagnostic procedures are used for its detection, for example, immunofluorescence, HA, HI and PCR and this is an important technique because it is very specific and sensitive. In this work, the presence of PPV in fetuses from swine farms with reproductive problems was detected by PCR. All of 170 samples from aborted fetuses, mummies or stillborns were sampled by PCR with primers designed to VP2 region of PPV and c-myc (endogenous control). Only 142 samples (83,53%) were positive for c-myc and among them six samples (4,22%) were positive for PPV which were tested in HA. In this test, erythrocytes suspension 1% was used and three samples (50%) agglutinated but they presented low titer (4). For this work, porcine parvovirus was detected in the samples analyzed by PCR and HA. It is important to emphasize the use of endogenous control when material with elevated degree of autolysis is examined

Infiltração coronária microbiana em canais radiculares obturados pela técnica do cone único com diferentes cimentos endodônticos

With the emergence of new filling materials with different properties and behaviors, the approach of endodontic treatment must be readjusted so that the appropriate result can be achieved. New endodontic sealers include methacrylate resin-based, plant resin-based and the evolution of epoxy-based sealers. This study verified the behavior of new materials that presents controversial results in the literature, about coronal bacterial leakage. That for, 56 single-rooted human teeth were prepared in the direction crown-apex and filled with gutta-percha points with taper of 4% using the single cone technique. Roots were divided randomly into 4 groups according to the sealer (Apexit Plus, AH Plus, EndoREZ and Polifil). After filling, the roots were incorporated in a leakage model, which upper chamber contained a suspension of Streptococcus mutans, and lower chamber a broth, leaving 3 mm of root apical portion immersed. Leakage was assessed for turbidity in lower chamber every day for 60 days. Survival analysis was performed using the nonparametric Kaplan- Meier method (p<0,05). All experimental groups presented leakage during the study’s period, however the maximum time achieve was 22 days. The medium time of leakage was: Apexit Plus 6,3 days, AH Plus 6,3 days and Polifil 5,1 days, but in EndoREZ all specimens infiltrated in the first day, presenting shorter capacity of impermeabilization compared to the other groups. Concluding that none of the sealers tested was able to prevent coronal bacterial leakage