Evaluation of anti-inflammatory activity of derivatives from aerial parts of Baccharis uncinella

Context: Species of Baccharis exhibit antibiotic, antiseptic, and wound-healing properties, and have been used in the traditional medicine of South America for the treatment of inflammation, headaches, diabetes, and hepatobiliary disorders.Objective: To investigate the anti-inflammatory activity of organic phases from EtOH extract of the aerial parts of Baccharis uncinella DC (Asteraceae).Materials and methods: The crude EtOH extract from the aerial parts of B. uncinella was subjected to partition procedures and the corresponding CH(2)Cl(2) and EtOAc phases were subjected to several chromatographic separation procedures. Thus, these phases and their purified compounds were assayed for evaluation of anti-inflammatory activity.Results: The CH(2)Cl(2) phase from EtOH extract from B. uncinella contained two triterpenoids (oleanolic and ursolic acids) and one flavonoid (pectolinaringenin), whereas the respective EtOAc phase showed to be composed mainly by two phenylpropanoid derivatives (caffeic and ferulic acids). The CH(2)Cl(2) and EtOAc phases as well as their isolated compounds exhibited anti-inflammatory effects against inflammatory reactions induced by phospholipase A2 (from Crotalus durissus terrificus venom) and by carrageenan.Discussion and conclusion: The results suggested that the components obtained from partition phases of EtOH extract of B. uncinella could represent lead molecules for the development of anti-inflammatory agents. Additionally, the results confirmed the use of Baccharis genus in the traditional medicine of South America for the treatment of inflammation and other heath disorders. To date, the present work describes for the first time the anti-inflammatory effects of compounds isolated from B. uncinella.

Atividade antimalárica de plantas medicinais da biorregião do Araripe-CE em modelo murino - Plasmodium berghei

Malaria, also popularly known as maleita , intermittent fever, paludism, impaludism, third fever or fourth fever, is an acute infectious febrile disease, which, in human beings, is caused by four species: Plasmodium falciparum, P. vivax, P. malariae and P. ovale. Malaria, one of the main infectious diseases in the world, is the most important parasitoses, with 250 million annual cases and more than 1 million deaths per year, mainly in children younger than live years of age. The prophylactic and therapeutic arsenal against malaria is quite restricted, since all the antimalarials currently in use have some limitation. Many plant species belonging to several families have been tested in vivo, using the murine experimental model Plasmodium berghei or in vitro against P. falciparum, and this search has been directed toward plants with antithermal, antimalarial or antiinflammatory properties used in popular Brazilian bolk medicine. Studies assessing the biological activity of medicinal plant essential oils have revealed activities of interest, such as insecticidal, spasmolytic and antiplasmodic action. It has also been scientifically established that around 60% of essential oils have antifungal properties and that 35% exhibit antibacterial properties. In our investigation, essential oils were obtained from the species Vanillosmopsis arborea, Lippia sidoides and Croton zethneri which are found in the bioregion of Araripe-Ceará. The chemical composition of these essential oils was partially characterized and the presence of monoterpenes and sesquiterpenes. The acute toxicity of these oils was assessed in healthy mice at different doses applied on a single day and on four consecutive days, and in vitro cytotoxicity in HeLa and Raw cell lines was determined at different concentrations. The in vivo tests obtained lethal dose values of 7,1 mg/Kg (doses administered on a single day) and 1,8 mg/Kg (doses administered over four days) for 50% of the animals. In the in vitro tests, the inhibitory concentration for 50% of cell growth in Hela cell lines was 588 μg/mL (essential oil from C. zethneri after 48 h), from 340-555 μg/mL (essential oil from L. sidoides, after 24 and 48 h). The essential oil from V. arborea showed no cytotoxicity and none of the essential oils were cytotoxic in Raw cell lines. These data suggest a moderate toxicity in the essential XVIII oils under study, a finding that does not impede their testing in in vivo antimalarial assays. Was shown the antimalarial activity of the essential oils in mice infected with P. berghei was assessed. The three species showed antimalarial activity from 36%-57% for the essential oil from the stem of V. arborea; from 32%-82% for the essential oil from the leaves of L. sidoides and from 40%-70% of reduction for the essential oil from the leaves of C. zethneri. This is the first study showing evidence of antimalarial activity with these species from northeast Brazil. Further studies to isolate the active ingredients of these oils are needed to determine if a single active ingredient accounts for the antimalarial activity or if a complex integration of all the compounds present occurs, a situation reflected in their biological activity

Leguminosae na Unidade de Conservação Refúgio de Vida Silvestre Pedra da Andorinha, Sobral, Ceará, Brasil

Leguminosae com cerca de 650 gêneros e 19.000 espécies compreende a terceira maior família de angiospermas, superada apenas por Orchidaceae (20.000 spp.) e Asteraceae (24.000 spp.). Para o Brasil, são citados 222 gêneros e 2.803 espécies distribuídas nos diversos domínios fitogeográficos, e para região Nordeste estão registradas 1.085 espécies em 168 gêneros. No Ceará, são reportadas 340 espécies em 105 gêneros, dos quais Mimosa L. (26 spp.) é o mais diversificado. No bioma Caatinga, é a principal família de fanerógamas com 83 gêneros e 354 espécies, muitas das quais com diversas potencialidades de uso. Em virtude da importância deste táxon no contexto do semiárido, objetivou-se com este trabalho catalogar as Leguminosae ocorrentes em uma área de Caatinga no distrito de Taperuaba, Sobral, Ceará. O estudo foi realizado na Unidade de Conservação Refúgio de Vida Silvestre Pedra da Andorinha, localizada a 70 km da sede municipal e possuindo aproximadamente 600 ha. A área é composta por Caatinga em duas fitofisionomias: uma terrícola e outra rupícola. As expedições de coletas foram realizadas entre março/2015 e março/2016. Os espécimes foram identificados com o auxílio de literatura e sites especializados. O material encontra-se depositado no acervo do Herbário Prof. Francisco José de Abreu Matos (HUVA). Foram catalogadas 15 espécies em oito gêneros: Mimosa (4 spp.), Chamaecrista Moench (3 spp.), Poincianella Britton & Rose (2 spp.). Bauhinia L., Centrosema (DC.) Benth., Desmodium Desv., Senna Mill., Stylosanthes Sw. e Zornia J.F. Gmel. apresentaram uma espécie cada. Dentre as espécies coletadas, duas apresentam propriedades medicinais: B. forficata (Vogel) Fortunato & Wunderlin e M. tenuiflora (Willd.) Poir. Centrosema pascuorum Mart. ex Benth, Mimosa pudica (Humb. & Bonpl. ex Willd.) DC., Stylosathes humilis Kunth e Zornia cearensis Huber são tratadas como invasoras de cultivos. Entre as espécies com alto potencial forrageiro, estão Chamaecrista linearis (H.S. Irwin & Barneby) Afr. Fern. & E.P. Nunes e Desmodium tortuosum (Sw.) DC. Por outro lado, Senna obtusifolia (L.) H.S. Irwin & Barneby, é tóxica aos bovinos. Alguns estudos ainda sugerem que os extratos das raízes de Poincianella bracteosa (Tul.) L.P. Queiroz possuem atividade biológica sobre larvas de Aedes aegypti. Diante dos resultados, verificou-se que Leguminosae é a família mais representativa da área de estudo e que 11 espécies apresentaram algum uso ou potencialidade econômica.

A New Species of Paracoccus Ezzat and McConnell (Hemiptera: Pseudococcidae) from Southern Brazil.

Resumo: O gênero mealybug Paracoccus Ezzat & McConnell (Hemiptera: Coccomorpha : ococcidae Pseud ) em todo o mundo tem oito espécies neotropicais descritas, incluindo duas espécies conhecidas do Brasil. Neste artigo, descrevemos uma terceira espécie do Brasil: Paracoccus galzerae Pacheco da Silva & Kaydan sp. n . , com base na morfologia de fêmeas adultas coletadas sobre as raízes da Conyza bonariensis (Asteraceae ) em vinhedos na cidade de Bento Gonçalves, Rio Grande do Sul. Uma chave de identificação revista incluindo a nova espécie é fornecida para a região Neotropical. Abstract: The worldwide mealybug genus Paracoccus Ezzat & McConnell (Hemiptera: Coccomorpha: Pseud ococcidae) has eight described Neotropical species, including two species known from Brazil. In this article, we describe a third species from Brazil: Paracoccus galzerae Pacheco da Silva & Kaydan sp. n., based on the morphology of adult females collected on the roots of Conyza bonariensis (Asteraceae) in vineyards in Bento Gonçalves City, Rio Grande do Sul. A revised identification key including the new species is provided for the Neotropical region.