Molecular Medicine and Molecular Pathology for Hematologists:II. Lets go Techno; Principles of the Molecular Technologies and their Applications in Haematology

Molecular techniques have a key role to play in laboratory and clinical haematology. Restriction enzymes allow nucleic acids to be reduced in size for subsequent analysis. In addition they allow selection of specific DNA or RNA sequences for cloning into bacterial plasmids. These plasmids are naturally occuring DNA molecules which reside in bacterial cells. They can be manipulated to act as vehicles or carriers for biologically and medically important genes, allowing the production of large amounts of cloned material for research purposes or to aid in the production of medically important recombinant molecules such as insulin. As acquired or inherited genetic changes are implicated in a wide range of haematological diseases, it is necessary to have highly specific and sensitive assays to detect these mutations. Most of these techniques rely on nucleic acid hybridisation, benefitting from the ability of DNA or RNA to bind tighly to complimentary bases in the nucleic acid structure. Production of artificial DNA molecules called probes permits nucleic acid hybridiation assays to be performed, using the techniques of southern blotting or dot blot analysis. In addition the base composition of any gene or region of DNA can be determined using DNA sequencing technology. The advent of the polymerase chain reaction (PCR) has revolutionised all aspects of medicine, but has particular relevance in haematology where easy access to biopsy material provides a wealth of material for analysis. PCR permits quick and reliable manipulation of sample material and its ability to be automated makes it an ideal tool for use in the haematology laboratory.

Molecular Medicine and Molecular Pathology for Haematologists:I. Gene Speak made Easy: An Overview of Genes and Gene Expression

Molecular Medicine and Molecular Pathology are integral parts of Haematology as we enter the new millennium. Their origins can be linked to fundamental developments in the basic sciences, particularly genetics, chemistry and biochemistry. The structure of DNA and the genetic code that it encrypts are the critical starting points to our understanding of these new disciplines. The genetic alphabet is a simple one, consisting of just 4 letters, buts its influence is crucial to human development and differentiation. The concept of a gene is not a new one but the Human Genome Project (a joint world-wide effort to characterise our entire genetic make-up) is providing an invaluable understanding of how genes function in normal cellular processes and pinpointing how disruption of these processes can lead to disease. Transcription and translation are the key events by which our genotype is converted to our phenotype (via a messenger RNA intermediate), producing the myriad proteins and enzymes which populate the cellular factory of our body. Unlike the bacterial or prokaryotic genome, the human genome contains a large amount of non coding DNA (less than 1% of our genome codes for proteins), and our genes are interrupted, with the coding regions or exons separated by non coding introns. Precise removal of the intronic material after transcription (though a process called splicing) is critical for efficient translation to occur. Incorrect splicing can lead to the generation of mutant proteins, which can have a dilaterious effect on the phenotype of the individual. Thus the 100,000-200,000 genes which are present in each cell in our body have a defined control mechanism permitting efficient and appropriate expression of proteins and enzymes and yet a single base change in just one of those genes can lead to diseases such as haemophilia or fanconis anaemia.

Revisiting Iris Marion Young on Normalisation, Inclusion and Democracy

Vieten's edited collection brings together papers that were given at the I M Young Memorial Symposium 'Inclusion and Democracy Revisited', held in Amsterdam in 2012. The different chapters presented explore in-depth, Young's models of a 'politics of cultural difference', and a 'politics of positional difference' read in combination with her critique of normalisation. Young regards the latter as decisive to any change for the better when reaching out politically to a fairer and more just democratic society.
With the current political, economic and socio-cultural crisis in mind, the contemporary world of global speed and transformed societies in and beyond Europe needs a refinement of what we understand 'normalisation' and 'difference' to be. How can we connect to each other, and in what ways can Young's 'structural inequality model' be applied to develop alternative outlooks on how to enhance inclusion and democracy in different nation states?

An elegant way to increase acidity in SAPOs: use of methylamine as co-template during synthesis

SAPO-34 and SAPO-11 with an increased number of acid sites were synthesized by adding methylamine (MA) as co-template to tetraethylammonium hydroxide (TEAOH) or dipropylamine (DPA) respectively used as true templates.

Methylamine as true template and TEAOH as purifying agent: unexpected roles of current organic additives in the hydrothermal synthesis of microporous aluminophosphates

Methylamine (MA), TEA+ and water were shown to play a concerted role during the synthesis of two new aluminophosphates IST-1 and IST-2. Both structures start to nucleate after the dramatic change of the gel composition due to preliminary interactions between TEA+ cations.

Unusual framework stabilization of Cu(II) and Cu(I) ions in a novel copper-substituted aluminophosphate with AEN topology prepared by one pot synthesis

A novel copper-substituted aluminophosphate (CuIST-2) has been synthesized using methylamine as a templating agent. X-Ray diffraction studies provide evidence for an AEN topology similar to the parent Cu-free IST-2.

Generation of acid sites by incorporation of cobalt in the AFR structure

Pure CoAPO4-40 and CoAPSO-40 samples have been synthesized. The CoII <=> CoIII framework transformation, and hence the number of potential acid sites has been evaluated by spectroscopic techniques and using m-xylene isomerization as model reaction.

A fauna do Novo Mundo e o dealbar da alimentação científica na Europa

As preocupações médicas com o equilíbrio alimentar remontam à Antiguidade, mas apenas a partir do século XVII o assunto começou a ser questionado de modo mais científico e preciso. Dois médicos holandeses de renome, Luís Nunes (1553-1645) e Willem Piso (1611-1678), estudaram esta questão e legaram-nos tratados de inquestionável relevância historiográfica. Destacamos, em particular, Ichtyophagia sive de piscium esu commentarius (“Ictiofagia ou comentário sobre uma alimentação piscívora”, Antuérpia, 1616) e De Indiae utriusque re naturali et medica. Libri quatuordecim (“Sobre a Índia e sua história natural e médica”, Amesterdão, 1658). A defesa de uma dieta que inclua o consumo de peixe é transversal aos dois textos, pois ambos fundam um discurso inaugural em defesa de hábitos alimentares equilibrados numa época de profundas mudanças históricas e culturais impostas pelo contacto com as realidades do exótico Novo Mundo. Esta influência é sobretudo evidente na obra de Piso, especialmente nas suas descrições de espécies de peixes endémicas do Brasil.