Operator and organizational maintenance manual : power unit, utility pack, gas turbine engine driven (Airesearch model PPU85-5), non-winterized, FSN 6115-937-0929 (Airesearch model PPU85-4), winterized, FSN 6115-134-0825.

"December 1968."

Operator, organizational, intermediate (field), (direct support and general support), and depot maintenance manual : generator sets, gasoline engine driven, 0.5 KW (less engine) : DOD model MEP-014A ....

"October 1977."

Operator, organizational, intermediate (field), (direct support and general support), and depot maintenance manual : generator sets, gasoline engine driven, 0.5 kw (less engine) : DOD model MEP-014A ....

"October 1977."

Direct and general support and depot maintenance repair parts and special tools lists : theodolite, directional, 1 sec graduation, 5.9 in. length telescope w/tripod, carrying case, and accessories (Wild Heerbrugg instruments), model T-2, serial numbers 5602 through 17897 and 27235 through 30594 (FSN 6675-232-8972), model T-2 56 ....

"September 1968."

Operator, organizational, direct support, general support, and depot maintenance manual : theodolite, directional, 0.2 mil graduation, 5.9 in. long telescope, w/accessories (Wild Heerbrugg models), (model T16-mil 66, type II), FSN 6675-926-4505 (model T16-mil 68, type II), FSN 6675-861-7939, theodolite, directional, 1 min graduation, 5.9 in. long telescope, w/accessories ....

"March 1970."

Direct and general support and depot maintenance repair parts and special tools lists : theodolite, directional; 0.002-mil graduation, 5.9 in. long telescope; detachable tribrach; w/accessories and tripod (Wild Heerbrugg model T-2-56-C-MIL), FSN 6675-682-4635 ... (Wild Heerbrugg model T-2-56-C-MIL, reference), FSN 6675-988-5225.

Includes index.

Operator, organizational, direct support and general support maintenance manual : theodolite, directional, 5.9 in. lg telescope, detachable tribrach, w/accessories and tripod (Wild Heerbrugg model T-16-0.2 mil) (FSN 6675-542-1683).

Includes index.

Direct and general support and depot maintenance, repair parts and special tools lists : theodolite, directional, 1 min. graduation, 5.9-in. long telescope w/accessories, (Wild Heerbrugg model T-16 type l) FSN 6675-828-6397.

Includes index.

Operator, organizational, field, and depot maintenance manual : theodolite, directional, 1 sec graduation, 5.9 in. length telescope, w/tripod, carrying case, and accessories (Wild Heerbrugg Instruments), model T-2, serial number ranges 5602 through 17897 and 27235 through 30594, FSN 6675-232-8972, model T-2 56 ....

"December 1961."

Operator, organizational, direct support, and general support maintenance manual : theodolite, directional, 0.002 mil graduation, 5.9 in. lg. telescope ... (Wild Heerbrugg models), (model T2-56-C-MIL), NSN 6675-00-682-4635 ... theodolite, directional (reference), (Wild Heerbrugg models T2-56-C-MIL ... and T2-68MIL), NSN 6675-00-988-5225.

Includes index.

Wood-preserving; a quarterly journal devoted to promoting the use of wood for all purposes to which it is adapted; the preservative treatment of wood wherever decay is a factor in its service; the development of materials and processes for effectively treating wood; the economical operation of wood-preserving plants ; and the general conservation of our forest resources. v.1-5; Jan. 1914.-Dec.1918.

Mode of access: Internet.

A macrophage colony-stimulating factor receptor-green fluorescent protein transgene is expressed throughout the mononuclear phagocyte system of the mouse

The c-fms gene encodes the receptor for macrophage colony-stimulating factor (CSF-1). The gene is expressed selectively in the macrophage and trophoblast cell lineages. Previous studies have indicated that sequences in intron 2 control transcript elongation in tissue-specific and regulated expression of c-fms. In humans, an alternative promoter was implicated in expression of the gene in trophoblasts. We show that in mice, c-fms transcripts in trophoblasts initiate from multiple points within the 2-kilobase (kb) region flanking the first coding exon. A reporter gene construct containing 3.5 kb of 5' flanking sequence and the down-stream intron 2 directed expression of enhanced green fluorescent protein (EGFP) to both trophoblasts and macrophages. EGFP was detected in trophoblasts from the earliest stage of implantation examined at embryonic day 7.5. During embryonic development, EGFP highlighted the large numbers of c-fms-positive macrophages, including those that originate from the yolk sac. In adult mice, EGFP location Was consistent with known F4/80-positive macrophage populations, including Langerhans cells of the skin, and permitted convenient sorting of isolated tissue macrophages from disaggregated tissue. Expression of EGFP in transgenic mice was dependent on intron 2 as no lines with detectable EGFP expression were obtained where either all of intron 2 or a conserved enhancer element FIRE (the Fms intronic regulatory element) was removed. We have therefore defined the elements required to generate myeloid- and trophoblast-specific transgenes as well as a model system for the study of mononuclear phagocyte development and function. (C) 2003 by The American Society of Hematology.

Increased potency of a novel complement factor 5a receptor antagonist in a rat model of inflammatory bowel disease

We have previously shown that complement factor 5a(C5a) plays a role in the pathogenesis of 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis in rats by using the selective, orally active C5a antagonist AcF-[OP(D-Cha) WR]. This study tested the efficacy and potency of a new C5a antagonist, hydrocinnamate (HC)-[OP(D-Cha) WR], which has limited intestinal lumenal metabolism, in this model of colitis. Analogs of AcF-[OP(D-Cha) WR] were examined for their susceptibility to alimentary metabolism in the rat using intestinal mucosal washings. One metabolically stable analog, HC-[OP(D-Cha)WR], was then evaluated pharmacokinetically and investigated at a range of doses (0.03 - 10 mg/kg/ day p.o.) in the 8-day rat TNBS- colitis model, against the comparator drug AcF-[OP(D-Cha) WR]. Using various amino acid substitutions, it was determined that the AcF moiety of AcF-[OP(D-Cha) WR] was responsible for the metabolic instability of the compound in intestinal mucosal washings. The analog HC-[OP( D-Cha) WR], equiactive in vitro to AcF-[OP(D-Cha) WR], was resistant to intestinal metabolism, but it displayed similar oral bioavailability to AcF-[OP(D-Cha) WR]. However, in the rat TNBS- colitis model, HC-[OP(D-Cha) WR] was effective at reducing mortality, colon edema, colon macroscopic scores, and increasing food consumption and body weights, at 10- to 30- fold lower oral doses than AcF-[OP( D-Cha) WR]. These studies suggest that resistance to intestinal metabolism by HC-[OP(D-Cha) WR] may result in increased local concentrations of the drug in the colon, thus affording efficacy with markedly lower oral doses than AcF-[OP(D-Cha) WR] against TNBS-colitis. This large increase in potency and high efficacy of this compound makes it a potential candidate for clinical development against intestinal diseases such as inflammatory bowel disease.

Regulation of albumin endocytosis by PSD95/Dlg/ZO-1 (PDZ) scaffolds - Interaction of Na+-H+ exchange regulatory factor-2 with ClC-5

The constitutive reuptake of albumin from the glomerular filtrate by receptor-mediated endocytosis is a key function of the renal proximal tubules. Both the Cl- channel ClC-5 and the Na+-H+ exchanger isoform 3 are critical components of the macromolecular endocytic complex that is required for albumin uptake, and therefore the cell-surface levels of these proteins may limit albumin endocytosis. This study was undertaken to investigate the potential roles of the epithelial PDZ scaffolds, Na+-H+ exchange regulatory factors, NHERF1 and NHERF2, in albumin uptake by opossum kidney ( OK) cells. We found that ClC-5 co-immunoprecipitates with NHERF2 but not NHERF1 from OK cell lysate. Experiments using fusion proteins demonstrated that this was a direct interaction between an internal binding site in the C terminus of ClC-5 and the PDZ2 module of NHERF2. In OK cells, NHERF2 is restricted to the intravillar region while NHERF1 is located in the microvilli. Silencing NHERF2 reduced both cell-surface levels of ClC-5 and albumin uptake. Conversely, silencing NHERF1 increased cell-surface levels of ClC-5 and albumin uptake, presumably by increasing the mobility of NHE3 in the membrane and its availability to the albumin uptake complex. Surface biotinylation experiments revealed that both NHERF1 and NHERF2 were associated with the plasma membrane and that NHERF2 was recruited to the membrane in the presence of albumin. The importance of the interaction between NHERF2 and the cytoskeleton was demonstrated by a significant reduction in albumin uptake in cells overexpressing an ezrin binding-deficient mutant of NHERF2. Thus NHERF1 and NHERF2 differentially regulate albumin uptake by mechanisms that ultimately alter the cell-surface levels of ClC-5.