撒坝猪、大河猪染色体比较研究

采用外周血淋巴细胞培养方法,以及C-带、G-带和银染技术,对云南地方猪种撒坝猪、大河猪进行显带染色体研究。测量了各号染色体的相对长度、着丝点指数及臂比,Ag-NORs定位于7号和10号染色体。撒坝猪、大河猪的G-带带型与其它家猪相似,C-带和Ag-NORs具有多态性。撒坝猪和大河猪的C-带和Ag-NORs存在一定差异。

中国鼠兔一新种--片马黑鼠兔

报道了采自云南片马的鼠兔属1新种,以其体色在鼠兔属中最为深黑为其主要特征而命名为片马黑鼠兔Ochotona nigritia sp. nov.。正模标本保存在中国科学院昆明动物研究所,副模标本保存在云南省流行病防治研究所。

TMVA, a novel GPIb-binding protein, significantly prevents platelet microthrombi formation and prolongs discordant cardiac xenograft survival

In xenotransplantation, donor endothelium is the first target of immunological attack. Activation of the endothelial cell by preformed natural antibodies leads to platelet binding via the interaction of the glycoprotein (GP) Ib and von Willebrand factor (vWF). TMVA is a novel GPIb-binding protein purified from the venom of Trimeresurus mucrosquamatus. In this study, the inhibitory effect of TMVA on platelet aggregation in rats and the effect on discordant guinea pig-to-rat cardiac xenograft survival were investigated. Three doses (8, 20 or 40 mug/kg) of TMVA were infused intravenously to 30 rats respectively. Platelet aggregation rate was assayed 0.5, 12, and 24 h after TMVA administration. Wister rats underwent guinea pig cardiac cervical heterotopic transplantation using single dosing of TMVA (20 mug/kg, i.v., 0.5 h before reperfusion). Additionally, levels of TXB2 and 6-keto-PGF(1alpha) within rejected graft tissues were determined by radioimmunoassay. Treatment with TMVA at a dose of 20 or 40 mug/kg resulted in complete inhibition of platelet aggregation 0.5 h after TMVA administration. Rats receiving guinea pig cardiac xenografts after TMVA therapy had significantly prolonged xenograft survival. Histologic and immunopathologic analysis of cardiac xenografts in TMVA treatment group showed no intragraft platelet microthrombi formation and fibrin deposition. Additionally, the ratio of 6-keto-PGF(1alpha) to TXB2 in TMVA treatment group was significantly higher than those in control group. We conclude that the use of this novel GPIb-binding protein was very effective in preventing platelet microthrombi formation and fibrin deposition in a guinea pig-to-rat model and resulted in prolongation of xenograft survival. The increased ratio of PGI(2)/TXA(2) in TMVA treatment group may protect xenografts from the endothelial cell activation and contribute to the prolongation of xenograft survival.

人工湿地中试系统基质中藻类组成的研究

从2003年3月到2004年2月,在水力负荷为600mm/d的条件下研究了人工湿地中试系统(mediumscaleplot,MSP)基质中藻类的组成.结果表明,栅藻、小球藻、盘星藻、空星藻、纤维藻、舟行藻、小环藻、平裂藻和颤藻属的一些种类在下行池基质各层不同季节均有出现,下行池表层藻类较丰;上行池基质中不同季节的藻类组成中念珠藻和鱼腥藻均为固氮种类,菱板藻、舟行藻和卵形藻属的一些种类在上行池表层出现较多,上行池藻类组成较下行池少.本实验探讨了在人工湿地基质中存在的藻类组成,为湿地除藻机理和评估系统的稳定性