Análise químico-farmacêutica de cápsulas de glimepirida

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Caracterização morfológica e fenotípica de leveduras do gênero Cryptococcus com ênfase na pesquisa de Cryptococcus neoormans isoladas de amostras de água de mar e areia de três ambientes costeiros do estado de São Paulo: Canal de São Sebastião, Baixada Santista e Ubatuba

The yeast Cryptococcus neoformans is the etiologic agent of cryptococcosis, an infectious cosmopolitan disease that affects humans. Although rare, this disease is potentially fatal, especially for immunocompromised hosts. This pathogen is frequently isolated from excrements of pigeons and parrots, with many environmental sources such as birds, pigeon droppings, eucalyptus leaves, decaying trees, towers, churches and places of storage of grain (the port area). The isolation of this microorganism has been obtained also from the aquatic environment. The identification of environmental sources is needed to protect human health, especially susceptible populations such as immunocompromised. Therefore, this study investigated the presence of Cryptococcus neoformans in yeast isolates obtained from samples of sea water and sand from three regions of São Paulo: São Sebastião Channel, Santos and Ubatuba. Isolates were analyzed according to micro-and macroscopic characteristics and biochemical tests: microculture, urease, ink nankin, auxanograma, zymogram and phenol. We analyzed 199 isolates, 175 of which had features suggestive for Cryptococcus spp. in microculture. All these 175 isolates were sown in the Christensen urea middle to verify the production of urease and submitted to the technique nankin ink to visualize the capsule. Of these, only 24 were selected for the next test that was the auxanograma (assimilation of carbohydrate and nitrogen). Of the 24, 10 were tested in zymograms (fermented sugar), from which 5 were selected for the phenoloxidase test in medium containing dopamine. None of the 5 isolates tested had black or brown color characteristic of Cryptococcus neoformans. According to these tests, we arrived at 5 isolates identified to the genus Cryptococcus, but not the neoformans specie

Principais marcadores utilizados na avaliação da qualidade do sêmen de animais domésticos

Methods of semen cryopreservation allow changes in spermatic cells, such as damage in plasma and acrossomal membrane and modifications in mitochondrial function due to a disorder in the lipidic bilayer. For effective oocyte fertilization, spermatozoa require functional competent membranes, and intact organelles, acrosome and DNA. However, most laboratory methods used to evaluate semen quality are not highly correlated with fertilizing capacity. The discovery of a variety of fluorochromes and compounds conjugated to fluorescent probes has enabled an accurate assessment of the viability, integrity and function of spermatozoa. Among the most used probes that label the various compartments of the sperm cell there are the membrane impermeable fluorescent dyes to test the membrane integrity, as well as acylated dyes that pass the intact membrane. For the acrossomal integrity the most commonly used method is lectins labeled by a fluorescent probe. The acrosome reaction and spermatic capacitation is detected by the evaluation of membrane architecture and disorder of lipids in plasma membrane. Mitochondrial function can be determined using markers for their aerobic activity. The DNA status of spermatozoa has been determined using the metachromatic properties of Acridine Orange, and the DNA fragmentation can also be assessed by TUNEL assay. Finally, DNA condensation is analyzed using a single cell DNA gel electrophoresis assay that indicates DNA compactation. This monograph aims to compile the various tests used to detect damaged spermatozoa under cryopreservation methods, searching for improve the predictive value of semen analysis with the intention of a successful conception

Efeito do diabetes sobre o crescimento puberal da próstata de rato: avaliação hormonal, imunocitoquímica e bioquímica

Adequate testosterone levels are necessary for the development, growth and maintenance of the male reproductive system. Testosterone deficiency is common in men with diabetes in whom it may contribute to impaired performance, with consequent reduction of the activity of the androgen regulated organs, such as the prostate. However, little attention has been given to the plasma dihydrotestosterone (DHT) level, the most potent androgen, nor to the expression of the androgen receptor (AR), insulin-like growth factor type I (IGF-1) and receptor (IGF-1R) in target tissues. Here, we investigated the effect of type I diabetes mellitus on DHT plasma levels and on prostate AR, IGF-1 and IGF-1R expression during rat pubertal growth. Diabetes was induced in prepubertal male rats through administration of streptozotocin (STZ; 40 mg/kg). Diabetic, diabetic treated with insulin, and age-matched control animals were killed by overdoses of pentobarbital. The ventral prostatic lobe (VP) was dissected, weighed and processed for immunohistochemistry for AR, IGF-1 e IGF-1R; plasma T and DHT levels were also determined. Hyperglycemia at puberty reduced VP weight gain to about 50% and plasma T level to about 80% of the control levels. In contrast there were no changes in plasma DHT levels. Insulin replacement restored the VP weight gain, but not the plasma T levels, which remained 90% below the ones of controls. Immunohistochemistry showed that AR, IGF-1 and IGF-1R expression in the prostate epithelial cells did not change with hyperglycemia or insulin replacement. Thus, the AR expression in the prostate epithelial cells appears to be regulated by DHT, and to a minor extent it also controls glandular growth

Análise do desempenho físico-mecânico de painéis compensados de Pinus sp. produzidos com adesivos aquosos a base de poliacetato de vinila e de homopolímero vinílico

This study aimed to evaluate the physico-mechanical performance of three PVA adhesive formulations according to the Brazilian standards for plywood testing and to evaluate the results with those presented in the technical literature and specified in normative documents. The study presents the production process of the panels, the obtaining of samples and, finally, the implementation of physico-mechanical characterization tests based on plywood Brazilian standards. For the panels production it was used a gramature of 120 g / m² and applied a pressure of 15 bars and two temperature combinations and pressing time for each adhesive. The commercial adhesives used were PVA 1810, PVA 1060 and Vinyltec 239. The physical tests conducted were thickness swelling, density, moisture content and water absorption. The mechanical tests were static flexion, for the determination of elasticity modulus (MOE) and rupture modulus (MOR), and shear in the glue line. Among the three formulations studied none showed good performance in the presence of water. However, other trials in the adhesive showed better results and closer to the commercially equivalent used, in the case the urea-formaldehyde, was the PVA 1810, that can be applied in non-structural uses and in environments without the presence of moisture

Determinação da temperatura para inativação da urease em amostras de casca e farelo de soja

s not degraded in the rumen but destroy ureasis. Soybean meal is one of the protein ingredients commonly used in formulations of animal feeds. In the diets of monogastric bran has high protein content provided by a greater separation of soybean hulls. For the ruminant protein value of the meal is lower with the inclusion of soybean hulls to reduce the level of protein. With the increased consumption of meal high protein, due to increases in production of pigs and poultry in recent years, increasing the availability of soybean hulls on the market, this is because soybean hulls is little understood in the feeding of monogastric . In this context, the main objective of this study was to determine the times and temperatures needed to ensure the effective inactivation of ureasis present in the bran and soybean hulls. According to the assessments, to make the determination of temperatures and times required for the inactivation of ureasis present in the bran and soybean hulls are 170 º C and 25 minutes for soybean meal and 140 ° C 10 minutes for soybean hulls, respectively

Metaloproteinases da matriz 2 e 9 em coelhos com cardiomiopatia induzida pela doxorrubicina

Pós-graduação em Medicina Veterinária - FCAV

Estresse oxidativo em cães com doença renal crônica

Pós-graduação em Medicina Veterinária - FCAV

Avaliação da infusão contínua de lidocaína em equinos submetidos à distensão ileal

Pós-graduação em Cirurgia Veterinária - FCAV

Efeito da ingestão aguda de taurina sobre o desempenho físico e metabolismo lipídico de nadadores de elite de Ribeirão Preto

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Prostaglandina E2 em cães portadores de linfoma multicêntrico submetidos ao protocolo quimioterápico de Madison-Wisconsin associado ou não a um inibidor de COX-2

Pós-graduação em Medicina Veterinária - FCAV

Leucograma e perfil bioquímico sérico de cabras com mastite experimental causada por Staphylococcus aureus e Streptococcus agalactiae

Pós-graduação em Medicina Veterinária - FCAV

Efeito da hCG ou deslorelina sobre a hemodinâmica folicular e perfil endogeno de LH em éguas cíclicas

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Frequências de alimentação sobre o comportamento ingestivo, digestibilidade do amido e flutuação de consumo em bovinos Nelore confinados

Pós-graduação em Ciência e Tecnologia Animal - FEIS

Nucleotídeos e ácido glutâmico em dietas de leitões desmamados

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)