Exposição de culturas de fibroblastos de pterígios primários e recidivados à mitomicina c e ao 5-fluorouracil

Purpose: To evaluate the proliferation of the fibroblasts from primary and recurrent pterygium in culture of cells, after the exposure to mitomycin C and to 5-fluorouracil. Methods: Cultures of fibroblasts from primary and recurrent pterygiuns had been carried through. The cells of the third passage were exposed to mitomycin C 0.4% during 3 minutes and to 5-fluorouracil 25mg/ml that was kept in the nutritional medium. After 3, 6, 12 and 18 days of the exposure, cell countings were made in hemocytometer, in triplicate, with a control not treated with the drugs for each day. The data were submitted to statistical analysis.Results: Mitomicyn C had homogeneous behavior in the primary and recurrent groups, which inhibited the cellular proliferation since the first counting day. However, with 5-fluorouracil, the proliferation inhibition was verified only after the third day of evaluation (in the second counting day) in the recurrent group, and since the first counting day in the primary group. At the end of the experimental period, 5-fluorouracil and mitomycin C were equally efficient in the inhibition of the fibroblasts from primary and recurrent pterygium proliferation. In the controls not exposed, the cell's numbers were increasing during the experimental time.Conclusion: Mitomycin and 5-fluorouracil are equally able to inhibit fibroblasts proliferation from primary and recurrent pterygium Tenon's capsule in cell culture.

Avaliação histológica da pele após exposição a gel acrescido de hialuronidase associado ou não a ultra-som

The objective of this work was to verify the effect in the skin of male swines gel (G) containing hyaluronidase (H) associated or not to ultrasound (US). In different areas was applied G; G+US; G+H; G+H+US and mesotherapy (M). Skin fragment was processed in paraffin. To evidence hyaluronic acid (HA) coloration with Alcian Blue (AB) was used and coloration with Hematoxilin/Eosin for morphometry. It was observed that G+H and G+H+US did not reduce coloration for the AB nor presented significant differences for the morphometry. When H was applied mesoteraphycally coloration for the AB diminished. Then, the use of H associated or with US did not seem efficient in the HA reduction.

Interação entre grau de perda auditiva e o incômodo com zumbidos em trabalhadores com história de exposição ao ruído

Hearing loss and tinnitus impact the lives of workers in every instance of their lives. Aim: this paper aims to investigate the existence of a dose-response relationship between hearing loss and tinnitus by determining whether higher levels of hearing loss can be associated with increased tinnitus-related discomfort. Materials and method: this cross-sectional case study assessed 284 workers exposed to occupational noise through pure tone audiometry. Test results were categorized as defined by Merluzzi. Individuals complaining of tinnitus answered the adapted and validated Brazilian Portuguese version of the Tinnitus Handicap Inventory. A generalized linear model was adjusted for binomial data to test the interaction between these factors. Results: over 60% of the ears analyzed had hearing loss, while more than 46% of them had tinnitus. Tinnitus prevalence and risk rates increased as pure tone audiometry results got worse. The association between both, considering all hearing loss degrees, was statistically significant. Conclusion: the results point to a statistical association between hearing loss and tinnitus; the greater the hearing loss, the greater the discomfort introduced by tinnitus. 2009 © Revista Brasileira de Otorrinolaringologia.

Treino prévio reduz o tempo de execução de tarefas visuo-espaciais em ambiente virtual

Objective. To evaluate the influence of previous adaptation to different computational environments in visuo-spacial tasks performance of healthy individuals. Method. Healthy volunteers (n = 30), 15 male, mean age 25.3 ± 3.3 years, were divided in three groups: the first group, considered control, was not adapted to the proposed environments; the second group was adapted to a closed environment (stable and expected), and the third group was adapted to an open environment A (unexpected). The proposed task was to go through two open environments B and C (maze). The dependent variables Time and Error were considered for the analysis. Results. It was observed that during the adaptation phase, in the Time variable, the groups presented a progressive improvement in the performance to each task (p = 0.0036). The group adapted in the A open environment, showed a tendency to be faster in the execution of B and C open environments tasks, than the group adapted in the closed environment (p = 0.068). Conclusion. The study suggests that subjects adapted to visuo-spacial tasks execution involving unknown and no guided situations, present a tendency to a better time performance in these tasks when compared to subjects adapted in fixed and guided situations.

Estimativa do tempo de vida útil de represa de pequeno porte

The purpose of this work is to compare two methods for time estimation of useful life for a small and shallow reservoir, located in Pirassununga, São Paulo State, Brazil: 1) sedimentometry; and 2) bathymetry. The model indicated a useful lifetime around 50 years for the studied dam. The values are between 51 years for the initial years of 1998/1999, and 46 years for the final years of 2004/2005, with an oscillation between 1999/2000 of 27 years, and between 2000/2001 of 76 years. The results show that it is possible to estimate the useful lifetime for small dams through the sedimentometric method, by knowing the correction coefficient value (K'). The coefficient is calculated using time values of useful lifetime calculated by the sedimentometric and bathymetric methods simultaneously, during the span of one year.

Dose de exposição radiométrica de granitos do estado de Rondônia, Brasil

This work evaluated the activity concentration of the radioelements K, eU and eTh in samples of granites from Rondônia State, Brazil. The statistical analysis of the data indicated that they fit lognormal distributions. The modal values correspond to about 11% for K, 29 ppm for eU and 85 ppm for eTh. Directsignificant correlations were found among the concentrations of the three radioelements, i.e. r = 0.71 (between K and eU), r = 0.72 (between K and eTh), andr = 0.72 (between eU and eTh), suggesting congruency of their accumulation in minerals occurring in the rocks analyzed. The activity concentration data allowed estimate the absorbed dose rate in air at 1 m above the ground, which also fits a lognormal distribution characterized by a mode of 2.7 mSv/y that is slightly higher than the average worldwide exposure of 2.4 mSv/y. The results obtained also allowed evaluate if the granites analyzed are radiometrically suitable as building and ornamental materials. © 2009 Sociedade Brasileira de Geofísica.

Efeito da temperatura e tempo de austenitização nas transformações de fase da liga 13Cr2Ni0,1C

New classes of martensitic stainless steels, with low carbon levels, have been developed aiming to meet the needs of the petroleum industry segment. However, their use has been restricted due to the fact it is a recent development and many of its properties are still under investigation. This work determines the values of initial and final temperatures for the austenitic transformation and the initial and final temperatures of martensitic formation for alloy 13Cr2Ni0,1C, by means of dilatometric tests under continuous cooling. Based on these results the optimized conditions for quench and temper heat treatments were obtained. The microstructural characterization of the alloys under coarse fusion condition was carried out by optical microscopy and the presence of delta-ferrite in the martensitic matrix was observed.

Avaliação do efeito do tempo de desossa sobre a qualidade da carne de peito de matrizes pesadas de descarte

The aim of this study was to evaluate the effect of deboning time on broiler breeder hen meat for in natura consumption. Eighty samples of breast fillets with bone (Pectoralis major muscle) were collected and divided into four groups of 20 samples each; each group represented a different post-mortem deboning time: 0 (immediately after cooling), 4, 8 and 12h post-mortem. At 24h post-mortem, all samples were evaluated for the following parameters: pH, color (L*, a* and b*), cooking loss, water retention capacity, shear force, myofibrillar fragmentation index and fiber diameter. Color (L*, a* and b*), cooking loss, water retention capacity and shear force were significantly different (p ≥ 0.05) among the evaluated deboning times. The shear force value of breast fillets deboned at 0h post-mortem (8.478) was significantly different (p ≥ 0.05) from those deboned at 4, 8 and 12h post-mortem (5.154; 5.375 and 4.819, respectively). Therefore, an increase in the tenderness of breast fillets was observed, when deboning was performed at least 4h post-mortem.

Digestibilidade e tempo de trânsito gastrintestinal de dietas contendo níveis crescentes de fibra bruta para pacu

This work aimed to evaluate the effect of increasing dietary fiber levels on the digestibility and gastrointestinal transit time (GTT) for pacu. The digestibility trial used 288 pacu in a completely randomized design, fed in aquaria and transferred to a modified Guelf-type feces collector, using the partial sampling method. The inert marker on the diets was chromic oxide (1%) to establish the diet digestibility. In the GTT assay, 288 pacu were distributed in 24 aquaria in a completely randomized design. Fish were fed with two colored diets - green and white - and feces color was determined after abdominal pressure. The white and green diets used 1% of titanium and chromic oxides, respectively. Total GTT was determined when all fish showed green feces. The apparent digestibility coefficients and GTT of diets decreased as dietary fiber levels increased. These results indicated that 9% dietary fiber can be used in pacu diets without negative effects on energy, protein, crude fiber and ether extract digestibility and GTT.

Mapeamento institucional e de medidas de política com impacto sobre a inovação produtiva e a diversificação das exportações

Includes bibliography

Mudanças estruturais na agricultura brasileira, 1980-1998

Incluye Bibliografía

Efeito da exposição à fumaça de cigarro durante a prenhez e a lactação de ratas e sua prole sobre parâmetros séricos e morfométricos

PURPOSE: to investigate the effect of cigarette smoke exposure on body and tissue weight gain, serum parameters and milk yield during pregnancy and lactation in rats, and the impact on offspring from birth toil young adulthood. METHODS: 40 Wistar pregnant rats were randomly divided into: CG - not exposed to cigarette smoke and sacrificed at the end of pregnancy; CL - not exposed to cigarette smoke and sacrificed at the end of lactation; FG - exposed to cigarette smoke and sacrificed at the end of pregnancy; FL - exposed to cigarette smoke and sacrificed at the end of lactation. The offspring were separated by gender and divided according to their mothers' groups. Tissue weight, body weight and serum parameters were evaluated in rats and offspring. Milk yield per pup was calculated. RESULTS: body weight was decreased in FL during lactation (CL=267.0±7.2; FL=235.5±7.2 g*, *p<0.05). Adipose tissue was not detected in the CL and FL groups, and was reduced in FG compared to CG (CG=3.3±0.3; FG=2.4±0.3 g*, *p<0.05). Rats exposed to cigarette smoke had higher blood glucose levels (CG=113±17, CL=86±16, FG=177±21*, FL=178±23 mg/dL*, *p<0.05 CG versus FG e CL versus FL), CL and FL groups presented lower HDL-cholesterol with no change in total cholesterol. Finally, rats exposed to cigarette smoke had lower milk yield compared to unexposed rats (CL=6.7±0.4, FL=5.4±0.3 g*, *p<0.05). In offspring from the FG and FL groups, there was a decrease of body weight from birth to young adulthood, with no changes in gastrocnemius, liver or heart weights in any group, and adipose tissue was no detected in female offspring. There was an increase in blood glucose in offspring of both sexes from rats exposed to cigarette smoke (males: Pcg=107±10.5, Pcl=115±8.6, Pfg=148±16.8*, Pfl=172±11.2**; females: Pcg=109±27.2, Pcl=104±9.7, Pfg=134±20.0*, Pfl=126±13.3**; p<0.05 *Pcg versus Pfg and **Pcl versus Pfl). CONCLUSIONS: exposure to cigarette smoke provokes impairment of morphometric and serum parameters during pregnancy and lactation both in mothers and offspring, which is maintained during young adulthood.

Alocação de fotoassimilados marcados e relação fonte-dreno em figueiras cv. Roxo de Valinhos. 2. Tempo de alocação

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Estimativa do tempo de produção de chorume em aterro controlado por meio de medidas de resistividade elétrica

Landfill is an engineering work that aims at to accommodate residues in the smallest possible space in the soil, with minimum damages to the environment and the public health. One in the constructive forms of a controlled landfill is the excavation of ditches with appropriate dimensions, for disposition of solid residues without compactation or impermeabilization. Liquid effluents generated for similar volumes of residues, disposed in ditches with ages different from closing, it should result in physical and chemical alterations you correlated at the time of residence materials. With base in this hypothesis, measures of electric resistivity were accomplished through the of Eletrical Resistivity geophysical method, on residues ditches closed annually between 12/2001 and 12/2007, localized in control landfill of the Cordeirópolis city (SP). The leachate is a liquid effluent generated by the decomposition of organic matter and characterized by high content in total dissolved solids, whose interaction with geological environment results in alterations in terms of electrical resistivity susceptible to detection for the geophysical instrumental. The results suggest a resistivity reduction for leachate percolation produced for ditches recently and increase progressive of the resistivity for older ditches. The statistical comparative analysis with reference values for the area suggests ages of the finish production and percolation leachate for soil and rock below the ditches.

Tempo de passagem de sementes pelo trato digestório de aves frugívoras, uma primeira avaliação no Brasil

The transit time of seeds ingested by frugivorous animals has important implications for the spatial distribution of seeds and their dispersal distance. Nevertheless, this parameter is rarely included in seed dispersal studies. In this paper, we provide information about the transit time of seeds of nine species of plants ingested by individuals of six species of captive birds (Turdus albicollis, T. amaurochalinus, T. leucomelas, T. rufiventris, Stephanophorus diadematus and Saltator similis). We found that (1) seeds are regurgitated quickly through the digestive tract of birds than defecated seeds, and (2) large seeds (e.g., > 5 mm in diameter for Turdus spp.) are regurgitated rather than defecated. These results corroborate other studies, but the relationship between seed size and transit time seems to be quite complex and variable, requiring more detailed studies on this important aspect of the ecology of seed dispersal and digestive physiology of frugivorous birds.