Surface plasmon resonance as a tool in the functional analisys of an immunodominant site in foot-and-mouth disease virus

A fast and direct surface plasmon resonance (SPR) method for the kinetic analysis of the interactions between peptide antigens and immobilised monoclonal antibodies (mAb) has been established. Protocols have been developed to overcome the problems posed by the small size of the analytes (< 1600 Da). The interactions were well described by a simple 1:1 bimolecular interaction and the rate constants were self-consistent and reproducible. The key features for the accuracy of the kinetic constants measured were high buffer flow rates, medium antibody surface densities and high peptide concentrations. The method was applied to an extensive analysis of over 40 peptide analogues towards two distinct anti-FMDV antibodies, providing data in total agreement with previous competition ELISA experiments. Eleven linear 15-residue synthetic peptides, reproducing all possible combinations of the four replacements found in foot-and-mouth disease virus (FMDV) field isolate C-S30, were evaluated. The direct kinetic SPR analysis of the interactions between these peptides and three anti-site A mAbs suggested additivity in all combinations of the four relevant mutations, which was confirmed by parallel ELISA analysis. The four-point mutant peptide (A15S30) reproducing site A from the C-S30 strain was the least antigenic of the set, in disagreement with previously reported studies with the virus isolate. Increasing peptide size from 15 to 21 residues did not significantly improve antigenicity. Overnight incubation of A15S30 with mAb 4C4 in solution showed a marked increase in peptide antigenicity not observed for other peptide analogues, suggesting that conformational rearrangement could lead to a stable peptide-antibody complex. In fact, peptide cyclization clearly improved antigenicity, confirming an antigenic reversion in a multiply substituted peptide. Solution NMR studies of both linear and cyclic versions of the antigenic loop of FMDV C-S30 showed that structural features previously correlated with antigenicity were more pronounced in the cyclic peptide. Twenty-six synthetic peptides, corresponding to all possible combinations of five single-point antigenicity-enhancing replacements in the GH loop of FMDV C-S8c1, were also studied. SPR kinetic screening of these peptides was not possible due to problems mainly related to the high mAb affinities displayed by these synthetic antigens. Solution affinity SPR analysis was employed and affinities displayed were generally comparable to or even higher than those corresponding to the C-S8c1 reference peptide A15. The NMR characterisation of one of these multiple mutants in solution showed that it had a conformational behaviour quite similar to that of the native sequence A15 and the X-ray diffraction crystallographic analysis of the peptide ? mAb 4C4 complex showed paratope ? epitope interactions identical to all FMDV peptide ? mAb complexes studied so far. Key residues for these interactions are those directly involved in epitope ? paratope contacts (141Arg, 143Asp, 146His) as well as residues able to stabilise a particular peptide global folding. A quasi-cyclic conformation is held up by a hydrophobic cavity defined by residues 138, 144 and 147 and by other key intrapeptide hydrogen bonds, delineating an open turn at positions 141, 142 and 143 (corresponding to the Arg-Gly-Asp motif).

International Summer School of Museology

Cadernos de Sociomuseologia Centro de Estudos de Sociomuseologia

As formas de participação na construção do projecto político pedagógico : diferentes realidades escolares

RESUMO: Na sociedade da comunicação e da informação, as escolas ainda não conseguiram compreender o seu papel de facilitadoras do conhecimento que o mundo está promovendo entre a realidade da escola e o interesse do educando. Um dos fatores para isso é o crescimento das escolas ao longo dos séculos e a necessidade de superação decorrente do alargamento da educação, que têm gerado um conhecimento complexo que descreve as reflexões e conscientizações sobre o nosso próprio desenvolvimento humano e sobre a nossa participação no mundo em que estamos vivendo. Essas complexidades possibilitam mudanças, principalmente nas questões epistemológicas e paradigmáticas, que proporcionaram outra forma de conceber o conhecimento e os saberes, chamado de “ecologia dos saberes”. Nesse contexto, existe a necessidade de se fazer uma leitura crítica na e da escola e de sua participação junto à comunidade escolar, objetivando minimizar as relações de dominação, de normatizações, de disciplinamento, de concentração de poder, para direcionar a educação num processo emancipatório, democrático, consciente das mudanças, de forma a incluir todos os saberes possíveis para acompanhar e valorizar a realidade escolar, através da participação de alta intensidade na construção e reconstrução do seu projeto político pedagógico. Para tanto, iremos discorrer sobre três escolas, a partir da análise de cada uma dentro da realidade construída por elas, buscando demonstrar que as escolas, dentro dos seus contextos educacionais, possuem diferentes realidades que não podem ser generalizadas, mas que devem ser compreendidas no sentido de que sejam promovidas a autonomia escolar e o fortalecimento da democracia educacional, objetivando a superação das condições atuais da educação para se adequar à sociedade e ao mundo atual. ABSTRACT: In the present society of communication and information, schools have not yet been able to understand their roles as facilitators of the knowledge that the world is promoting between the reality of school and the interest of the learner. One of the reasons for that is the growth of schools along the centuries as well as their constant need for getting over old problems, due to the expansion of education. These questions lead to a complex knowledge which describes reflections on our own human development and on our participation in the world we live in. These complexities lead to changes, especially in epistemological and paradigmatic questions, making it possible for new ways of conceiving knowledge, known as “ecology of knowledge”. In this context, there is a need for making a critical reading about school and its participation in the school community, with the objective to minimize the relations of domination, power concentration and discipline and to direct education towards a process of emancipation and democracy, in which there is conscience of the necessary changes to include all possible knowledge as a way to value the reality of school through a high intensity participation in the construction and reconstruction of its political and pedagogical project. Thus, this study will discuss three schools, basing this discussion on the analysis of each school within the reality built by them, trying to demonstrate that, within their educational contexts, each one possesses different realities that cannot be generalized. These differences must, however, be understood, in order to allow the promotion of school autonomy and the strengthening of educational democracy with the objective to get over education`s present conditions and adjust them to society and to the present world.

Metamorfoses em espelho de água : identidade, perfil, contextos e mudança nas (auto)representações dos professores

RESUMO: Nos últimos trinta anos, em Portugal, ocorreram processos de democratização política e de modernização da sociedade e das instituições, tendo como impulso as vontades nacionais e as mudanças ocorridas no Mundo em globalização, lideradas, no campo da educação, por agentes como a OCDE ou o Banco Mundial, e pela integração de Portugal na União Europeia. À implementação da(s) reforma(s), correspondeu uma mudança de paradigma educativo e organizacional, a criação de uma escola para todos, a emergência de novos alunos e de novos mandatos à Escola, a contingência de novas respostas educativas. Tais reformas constituiram instrumentos de mudança das organizações escolares e do sistema educativo, mas também do que significa ser professor, reformulando o desempenho e a “performatividade” docente (Ball, 2002), induzindo uma nova “identidade social” (Bernstein, 1996 e Dubar, 2006), produzindo novos modos de “fabricação da alma dos professores” (Foucault, 1996). Neste sentido, a autora procurou analisar, numa perspectiva crítica, as representações de professores do Ensino Básico, sobre os mecanismos de (re)configuração das suas identidades/perfis profissionais, recorrendo a uma investigação qualitativa descritiva, que privilegia a análise de conteúdo dos seus discursos sobre o tema, recolhidos segundo a técnica focus group. O estudo indiciou que os alunos são factor de realização, de risco e de mudança do perfil docente, actuando como uma quinta dimensão da (re)construção identitária dos Professores, a par da formação, do associativismo, do Estado e do Mercado, constituindo factor importante a ter em conta nos estudos sobre identidade docente. ABSTRACT: In the past thirty years, in Portugal, radical changes on politics and policies have been occurring, to achive the society and its institutions democratization and modernization, led by national wills and the changes occured in the World, stimulated, in the Education area, by global agencies like OECD, or the World Bank, and the integration of Portugal in the European Union. These reforms are connected to a new educational and organizational paradigm, the creation of a school for all, the emergence of new pupils, new demands to School and teachers, the imperative of new pedagogical solutions for educational problems, and are not only changing instruments in schools and in the educational system, but are also a powerful way to change “what to be a teacher” means, to re-formulate the teaching performance and “performativity” (Ball, 2002), to recompose his/her “social identity” (Bernstein, 1996; Dubar, 2006), or, in Michel Foucault (1996) words, to produce “new ways to manufacture teachers soul”. In this sense, the author intended to analyze, on a critical perspective, the representations of portuguese teachers of basic education (K12), on the mechanisms of (re)configuration of their professional identities/profiles, appealing to a qualitative descriptive research, which privileges the analysis of content of their speeches on the subject, collected according to the focus group technique, what, in its development, was brought near a circle of culture (in the sense of Paulo Freire‟s pedagogy). At least, pupils are the most important references and motivation to teachers changes, reflecting professional satisfaction and well done, but also risk, acting like a fifth dimension of teachers identity (re)construction, together with training, associative involvement, State and Market, and they must be considered on teatching identity studies.

Systems thinking: learning or driving?

The ability to foresee how behaviour of a system arises from the interaction of its components over time - i.e. its dynamic complexity – is seen an important ability to take effective decisions in our turbulent world. Dynamic complexity emerges frequently from interrelated simple structures, such as stocks and flows, feedbacks and delays (Forrester, 1961). Common sense assumes an intuitive understanding of their dynamic behaviour. However, recent researches have pointed to a persistent and systematic error in people understanding of those building blocks of complex systems. This paper describes an empirical study concerning the native ability to understand systems thinking concepts. Two different groups - one, academic, the other, professional – submitted to four tasks, proposed by Sweeney and Sterman (2000) and Sterman (2002). The results confirm a poor intuitive understanding of the basic systems concepts, even when subjects have background in mathematics and sciences.

Systems thinking: learning or driving?

The ability to foresee how behaviour of a system arises from the interaction of its components over time - i.e. its dynamic complexity – is seen an important ability to take effective decisions in our turbulent world. Dynamic complexity emerges frequently from interrelated simple structures, such as stocks and flows, feedbacks and delays (Forrester, 1961). Common sense assumes an intuitive understanding of their dynamic behaviour. However, recent researches have pointed to a persistent and systematic error in people understanding of those building blocks of complex systems. This paper describes an empirical study concerning the native ability to understand systems thinking concepts. Two different groups - one, academic, the other, professional – submitted to four tasks, proposed by Sweeney and Sterman (2000) and Sterman (2002). The results confirm a poor intuitive understanding of the basic systems concepts, even when subjects have background in mathematics and sciences.

Colmatando as dificuldades especificas do grupo e com o grupo no jardim de infância

RESUMO: Este trabalho é um relatório de um projecto de investigação-acção, no âmbito do Mestrado em Ciências da Educação - Educação Especial: domínio cognitivo e motor. É um trabalho de investigação que pretendeu implicar os vários intervenientes educativos e os próprios pares no desenvolvimento de uma criança, em idade pré-escolar, com atraso global do desenvolvimento e com problemas acentuados ao nível da linguagem, em contexto de sala de aula. Este trabalho foi realizado pela educadora da sala da aula. É um projecto que pretendeu promover o desenvolvimento da linguagem, através de um sistema de comunicação aumentativa, vocabulário Makaton. Este vocabulário foi utilizado por nós e pelas crianças do grupo em sala de aula, pela professora de educação especial, pelos técnicos que dão apoio à criança e pela família da referida criança. Pretendeu-se assim, promover o diálogo, a partilha de conhecimentos e os espaços escolares, entre os vários intervenientes educativos; professores, técnicos e família, com o intuito de desenvolver um trabalho assente em respostas inclusivas dirigidas à criança, aos seus pares e à sua família. Da partilha de conhecimentos e opiniões, resultou um trabalho para o grupo e com o grupo do jardim de infância. ABSTRACT:This work is one report of an action research project within the Master’s Degree in Educational Sciences - Special Education: motor and cognitive domain. It is a research project that seeks to highlight the role of the educational actors in the development of a child in preschool, with global developmental delay, with problems more pronounced at the level of language, in the context of the classroom. This work was carried out by the educator's classroom is a project that aims to promote language development through an augmentative communication system, Makaton vocabulary. This vocabulary was used by us and by the group of children in the classroom, the teacher of special education, by giving technical support to the child and the family of that child. It was intended thus to promote dialogue, knowledge sharing and school spaces between the various educational actors, teachers, coaches and family in order to develop a work based on inclusive responses directed to the child, their peers and his family . Sharing knowledge and opinions resulted in a job for the group and the group of kindergarten.

O trabalho em equipas docentes e o desenvolvimento da Educação Física

RESUMO: O sucesso das respostas aos novos desafios, exigências e expectativas que, nos dias de hoje, recaem sobre a escola, parece depender da concepção das estratégias organizacionais que globalmente a escola define, tendo em conta as características do contexto e das populações que serve. Depende por isso do modo como os actores chave se organizam e trabalham. Os modos de entender o trabalho docente em equipa prenunciam formas de entender a acção educativa e de conduzir a aprendizagem dos alunos. Tomando como ponto de partida as nossas experiências profissionais, este trabalho constitui uma reflexão sobre as possibilidades de desenvolvimento da Educação Física, baseada na escola e sustentada na organização do trabalho dos seus professores. Elaborando uma síntese dos principais problemas do seu desenvolvimento discutem-se as possibilidades e as vantagens da organização do trabalho dos professores em equipas, num contexto organizacional marcado por avanços e retrocessos nos processos de descentralização e atribuição de autonomia às escolas. Enquadra-se a organização do trabalho docente no âmbito das culturas de escola. Revisita-se o conceito de trabalho em equipa, distinguindo-o da simples participação nas estruturas da escola, realçando a sua importância na construção e gestão de conhecimento profissional através do estabelecimento de parcerias sinérgicas, onde o desempenho de papéis de liderança tem uma enorme importância. ABSTRACT: Successful responses to the new challenges, demands and expectations that currently are ascribed to schools seem to depend on the design of organizational strategies that broadly define the school, taking into account the characteristics of its context and the population it serves. Therefore, their success depends on how the key players are organized and work. The ways of perceiving the teaching team work forecast the ways of understanding the educational activity and how to lead learning. Starting from our own professional experience, this study is a reflection on the school based development of physical education, sustained by the organization of their teachers’ work. On organizing a summary of the major problems found in the development of this subject, we discuss the feasibilities and the gains got from the organization of teachers' teamwork, in an organizational context marked by promises and setbacks in the processes of decentralization and schools autonomy. The organization of teaching is to be seen as part of the school culture. We review the concept of teamwork as distinct from mere participation in the structures of the school, and its importance in the construction and management of professional knowledge will be highlighted by the establishment of synergistic partnerships, in which the performance of leadership roles is of the greatest importance. RÉSUMÉ: Le succès des réponses aux nouveaux défis, les exigences et les attentes que dans ces jours, s’accomplissent sur l'école, semble dépendre de la conception des stratégies d'organisation qui définit de façon générale l'école, en tenant compte des caractéristiques du contexte et des populations qu'elle dessert. Tout cela dépend donc de la façon dont les acteurs principaux sont organisés et travaillent. Les façons de comprendre l'équipe pédagogique démontrent des formes de comprendre l'activité éducative et de conduire à l'apprentissage des élèves. En prenant comme point de départ nos expériences professionnelles, ce travail est une réflexion sur le potentiel de développement de l'Éducation Physique, dépendante de l'école et soutenue dans l'organisation du travail de leurs enseignants. En préparant une synthèse des principaux problèmes de leur développement, on discute les possibilités et les avantages de l'organisation du travail des enseignants dans les équipes, dans un contexte organisationnel marqué par des avancées et des reculs dans le processus de décentralisation et l'accomplissement de l'autonomie aux écoles. On encadre l'organisation du travail des enseignants dans le champ des cultures de l’école. On revoit le concept du travail en équipe, en le distinguant de la simple participation dans les structures de l'école, en soulignant son importance dans la construction et gestion des connaissances professionnelles à travers l'établissement de partenariats synergiques, où les rôles de leadership joue un rôle d'une grande importance.

Inovação escolar e organização curricular

RESUMO: O presente estudo teve como objectivo investigar os principais impedimentos que ocorrem nos estabelecimentos de ensino relativamente à aplicabilidade dos Programas Nacionais de Educação Física (PNEF). Considerando as particularidades do grupo disciplinar, as instalações da escola ou o meio cultural envolvente, pretende-se perceber as razões para que no panorama nacional, a grande maioria das escolas resista a esse processo de mudança, não criando condições para o desenvolvimento de iguais oportunidades na disciplina de educação física no currículo de todos os alunos. Este estudo ambiciona ser um contributo para que a educação física (EF) dê um passo no desenvolvimento qualitativo da realidade educativa, ao destacar os pontos comuns e mais importantes reunidos nas escolas de referência que aplicam os PNEF. A metodologia desenvolvida apresenta duas fases na análise de dados, sendo que a primeira extrai os pontos em comum e personalizados do grupo de professores entrevistados pertencentes ao grupo A, constituído pelos autores dos PNEF e por uma especialista na área de desenvolvimento curricular. A segunda fase, segue a lógica anterior para o grupo B, grupo que reúne professores em contacto directo no dia-a-dia das escolas. A consciência e o conhecimento dos problemas apresentados nas escolas que não seguem os programas, torna-se importante pois possibilita a procura de soluções para esses mesmos problemas. Desta forma será possível desenvolver as inovações escolares, proporcionando aos alunos a oportunidade de crescer no seio das inúmeras qualidades que a disciplina de educação física pode oferecer e desenvolver. Um crescimento baseado na autonomia, na cooperação e no desenvolvimento de hábitos de vida saudável enquanto futuros cidadãos. ABSTRACT: This study aimed to investigate the main issues that prevent the physical education programs to be applied on the Portuguese schools. Considering the disciplinary group particularities, the school facilities or the environment, we intend to understand the reasons that may explain why the majority of the Portuguese schools resist to this change process, not creating conditions for the development of equal opportunities on the physical education of every student. This study intend to be a contribute for the physical education development on the educational reality, highlighting the common factors of the reference schools that apply the physical education programs. The method integrates two phases of development: the first one extracts the common and custom factors between the Group A professors, consisting of physical education programs authors and one expert in the field of curriculum development. The second phase follows the previous logic for the Group B, a group that meets professors with direct contact of schools day-to-day. The consciousness and knowledge about the existing problems in schools that don‟t apply physical education programs it‟s important, because it enables the search for solutions. This will allow innovations development, giving students the opportunity to grow within the many qualities that the discipline of physical education can provide and develop. Growth based on autonomy, cooperation and development of healthy lifestyle habits as future citizens.

Mortality problems in Brazil and in Germany: past-present-future. Learning from each other?

This article was written by a Swiss-German historical demographer after having visited different Brazilian Universities in 1984 as a guest-professor. It aims at promoting a real dialog between developed and developing countries, commencing the discussion with the question: Can we learn from each other? An affirmative answer is given, but not in the superficial manner in which the discussion partners simply want to give each other some "good advice" or in which the one declares his country's own development to be the solely valid standard. Three points are emphasized: 1. Using infant mortality in S. Paulo from 1908 to 1983 as an example, it is shown that Brazil has at its disposal excellent, highly varied research literature that is unjustifiably unknown to us (in Europe) for the most part. Brazil by no means needs our tutoring lessons as regards the causal relationships; rather, we could learn two things from Brazil about this. For one, it becomes clear that our almost exclusively medical-biological view is inappropriate for passing a judgment on the present-day problems in Brazil and that any conclusions so derived are thus only transferable to a limited extent. For another, we need to reinterpret the history of infant mortality in our own countries up to the past few decades in a much more encompassing "Brazilian" sense. 2. A fruitful dialog can only take place if both partners frankly present their problems. For this reason, the article refers with much emprasis to our present problems in dealing with death and dying - problems arising near the end of the demographic and epidemiologic transitions: the superanuation of the population, chronic-incurable illnesses as the main causes of death, the manifold dependencies of more and more elderly and really old people at the end of a long life. Brazil seems to be catching up to us in this and will be confronted with these problems sooner or later. A far-sighted discussion already at this time seems thus to be useful. 3. The article, however, does not want to conclude with the rather depressing state of affairs of problems alternatingly superseding each other. Despite the caution which definitely has a place when prognoses are being made on the basis of extrapolations from historical findings, the foreseeable development especially of the epidemiologic transition in the direction of a rectangular survival curve does nevertheless provide good reason for being rather optimistic towards the future: first in regards to the development in our own countries, but then - assuming that the present similar tendencies of development are stuck to - also in regard to Brazil.

The relationships between selected anthropometric and socio-economic data in schoolchildren from different social strata in Rio de Janeiro, Brazil

The nutritional status according to anthropometric data was assessed in 756 schoolchildren from 5 low-income state schools and in one private school in the same part of Rio de Janeiro, Brazil. The prevalence of stunting and wasting (cut-off point: <90% ht/age and <80% wt/ht) ranged in the public schools from 6.2 to 15.2% and 3.3 to 24.0%, respectively, whereas the figures for the private school were 2.3 and 3.5%, respectively. Much more obesity was found in the private school (18.0%) than in the state schools (0.8 - 6.2%). Nutritional problems seem to develop more severely in accordance with the increasing age of the children. Therefore it appears advisable to assess schoolchildren within the context of a nutritional surveillance system.

Foreign languages in the primary school

Of vital importance to the successful implementation of the teaching of FLs in the 1st Cycle...

Studies on mosquitoes (Diptera: Culicidae) and anthropic environment: 4 - Survey of resting adults and synanthropic behaviour in South-Eastern, Brazil

Resting adults Culicidae were collected from January 1992 through January 1993 in several habitats of the Ribeira Valley region. The diversity of species found among them suggested that the vegetation remaining within human settlements favored the survival and the population increase of some mosquitoes. Among there are: Ae. scapularis, Ae. serratus, Cx. (Culex), Cx. nigripalpus and Cx. (Melanoconion) such as Cx. ribeirensis. That preservation role may be attributed to the rearing of livestock and the consequent increase in the number of blood sources. These species may be classified as hemisynanthropes and Cx. oedipus apparently evolving to the eusynanthropic status. On the other hand, An. cruzii showed an asynanthropic behaviour, with a low degree of survival in the modified human environment. Epidemiological implications of the data are mentioned.