Crystallization and preliminary X-ray analysis of monalysin, a novel β-pore-forming toxin from the entomopathogen Pseudomonas entomophila.

Monalysin was recently described as a novel pore-forming toxin (PFT) secreted by the Drosophila pathogen Pseudomonas entomophila. Recombinant monalysin is multimeric in solution, whereas PFTs are supposed to be monomeric until target membrane association. Monalysin crystals were obtained by the hanging-drop vapour-diffusion method using PEG 8000 as precipitant. Preliminary X-ray diffraction analysis revealed that monalysin crystals belonged to the monoclinic space group C2, with unit-cell parameters a = 162.4, b = 146.2, c = 144.4 Å, β = 122.8°, and diffracted to 2.85 Å resolution using synchrotron radiation. Patterson self-rotation analysis and Matthews coefficient calculation indicate that the asymmetric unit contains nine copies of monalysin. Heavy-atom derivative data were collected and a Ta6Br14 cluster derivative data set confirmed the presence of ninefold noncrystallographic symmetry.

Iowa Department of Transportation Major Items - Preliminary Quantity Report, August 19, 2014; With Addendums

Weekly letting report.

Iowa Department of Transportation Major Items - Preliminary Quantity Report, August 19, 2014; With Addendums

Weekly letting report.

Iowa Department of Transportation Major Items - Preliminary Quantity Report, October 21, 2014; With Addendums

Weekly letting report.

Iowa Department of Transportation Major Items - Preliminary Quantity Report, September 16, 2014; With Addendums

Weekly letting report.

Iowa Department of Transportation Major Items - Preliminary Quantity Report, November 18, 2014; With Addendums

Weekly letting report.

Iowa Department of Transportation Major Items - Preliminary Quantity Report, December 16, 2014; With Addendums

Weekly letting report.

Iowa Department of Transportation Major Items - Preliminary Quantity Report, January 21, 2015; With Addendums

Weekly letting report.

Iowa Department of Transportation Major Items - Preliminary Quantity Report, December 16, 2014; With Addendums

Weekly letting report.

Good practice for dialogue and communication as strategic principle for policing political manifestations in Europe (GODIAC):field study handbook

This handbook describes the peer review methodology that was applied at the GODIAC project fi eld studies1. The peer review evaluation method as initiated by Otto Adang in the Netherlands and further developed in a European football context (Adang & Brown, 2008) involves experienced police offi cers cooperating with researchers to perform observational fi eld studies to identify good practices and learning points for public order management. The handbook builds on the GODIAC seminars and workshops, for the fi eld study members, which took place in September 2010, January 2012 and January 2013. The handbook has been discussed in the project group and in the steering committee. It is primarily written for the GODIAC fi eld study members as background material for understanding the fi eld study process and for clarifying the different responsibilities that enable active participation in the fi eld study. The handbook has been developed during the project period and incorporates learning points and developments of the peer review method. The handbook aims at promoting the use of fi eld studies for evaluation of policing major events.