Cloning and Characterization of Two Potent Kunitz Type Protease Inhibitors from Echinococcus granulosus

The tapeworm Echinococcus granulosus is responsible for cystic echinococcosis (CE), a cosmopolitan disease which imposes a significant burden on the health and economy of affected communities. Little is known about the molecular mechanisms whereby E. granulosus is able to survive in the hostile mammalian host environment, avoiding attack by host enzymes and evading immune responses, but protease inhibitors released by the parasite are likely implicated. We identified two nucleotide sequences corresponding to secreted single domain Kunitz type protease inhibitors (EgKIs) in the E. granulosus genome, and their cDNAs were cloned, bacterially expressed and purified. EgKI-1 is highly expressed in the oncosphere (egg) stage and is a potent chymotrypsin and neutrophil elastase inhibitor that binds calcium and reduced neutrophil infiltration in a local inflammation model. EgKI-2 is highly expressed in adult worms and is a potent inhibitor of trypsin. As powerful inhibitors of mammalian intestinal proteases, the EgKIs may play a pivotal protective role in preventing proteolytic enzyme attack thereby ensuring survival of E. granulosus within its mammalian hosts. EgKI-1 may also be involved in the oncosphere in host immune evasion by inhibiting neutrophil elastase and cathepsin G once this stage is exposed to the mammalian blood system. In light of their key roles in protecting E. granulosus from host enzymatic attack, the EgKI proteins represent potential intervention targets to control CE. This is important as new public health measures against CE are required, given the inefficiencies of available drugs and the current difficulties in its treatment and control. In addition, being a small sized highly potent serine protease inhibitor, and an inhibitor of neutrophil chemotaxis, EgKI-1 may have clinical potential as a novel anti-inflammatory therapeutic.

Da ideia de planejamento no Brasil ao plano diretor participativo de Rio Verde (GO)

v. 10, n. 1, p. 211-221, abr./2016.

Laadun ja oikean tiedon varmistaminen pk-yrityksen tilaus-toimitusketjussa

Oikean tiedon siirtyminen oikeaan aikaan, sekä laadukkaan työn tekeminen yrityksen tilaus-toimitusketjun jokaisessa vaiheessa, ovat avaintekijöitä arvolupauksen ja laadun täyttämiseen asiakkaalle. Diplomityön tavoite on kehittää pk-yritykselle työkalut parempaan tiedon hallintaan ja laadukkaan työn tekemiseen toiminnanohjausjärjestelmässä. Tutkimusmenetelmänä diplomityössä käytettiin toimintatutkimusta, jossa diplomityön tekijä osallistui kohdeyrityksen päivittäiseen työn tekemiseen neljän kuukauden ajan. Tutkimuksen tiedon keräämisessä käytettiin myös puolistrukturoitua haastattelua, sekä kyselytutkimuksella. Tutkimusote työssä on kvalitatiivinen eli laadullinen tutkimusote. Työ koostuu teoriaosasta sekä soveltavasta osasta, jonka jälkeen työn tulokset esitetään tiivistetysti johtopäätöksissä ja yhteenvedossa. Toiminnanohjausjärjestelmät keräävät ja tallentavat tietoa, jota työntekijät ja yrityksen rajapinnoilla työskentelevät ihmiset siihen syöttävät. Onkin äärimmäisen tärkeää, että yrityksellä on kuvatut yhtenäiset toimintamallit prosesseille, joita he käyttävät tiedon tallentamisessa järjestelmiin. Tässä diplomityössä tutkitaan pk-yrityksen nykyiset toimintamallit tiedon tallentamisesta toiminnanohjausjärjestelmään, jonka jälkeen kehitetään yhtenäiset ohjeet toiminnanohjausjärjestelmään syötetystä myyntitilaussopimuksesta. Teoriaosuudessa esitetään laatu eri näkökulmista ja mitä laadunhallintajärjestelmät ovat ja kuinka niitä kehitetään. Teoriaosassa myös avataan tilausohjautuvan tuotannon periaatteet, sekä toiminnanohjausjärjestelmän merkitys liiketoiminnalle. Teoriaosuudella pohjustetaan soveltavaa osuutta, jossa ongelma-analyysin jälkeen kehitetään yritykseen oma laadunhallintajärjestelmä, sekä uudet työmallit tiedonvaihtoon ja sen tallentamiseen. Tuloksena on myös toiminnanohjausjärjestelmän käytön tehostuminen ohjelmistotoimittajan tekemänä. Ohjelmasta karsittiin turhat nimikkeistöt ja sen konfigurointia tehostettiin. Työn tuloksena saatiin työohjeet ydinprosessien suorittamiseen, sekä oma laadunhallintajärjestelmä tukemaan yrityksen ydin- ja tukiprosesseja, sekä tiedonhallintaa.

O city marketing em Natal/RN e a construção da imagem da cidade

The growing importance of tourism in overall economic activity worldwide has favored the intensification of competition among cities that seek to create environments attractive to tourists and potential investors. It has been common practice to import characteristics of the business environment in the public management of cities. The city marketing is a key tool used by public leaders to promote a linkage between the tourism image and urban image and involves, in addition to promoting the image of the city, the planning of interventions in urban space, trying to formulate a positive image of the city able to facilitate the deployment of capital. This research seeks to understand the nature of city marketing as part of contemporary urban management and analyzes how is its application in decisions concerning the promotion of tourism in Natal/RN. The approach of this research is qualitative, exploratory and descriptive, in which respondents were the main leaders of two of the official tourism site, the Empresa Potiguar de Promoção Turística and the Secretaria Municipal de Turismo e Desenvolvimento Econômico. It was found that there is a strong articulation of public power with private enterprise in the design and conduct of the actions of urban marketing, that from the survey data show that the behavior of target markets provide guidelines for taking strategic decisions relating to tourism. Sun and sea are some key elements explored to form the image of Natal and to authorize the sale of the city as a tropical paradise. However, there is an increase in the diversification of tourism products, seeking to increase flow to the segments of ecotourism, adventure, business and culture. It s also growing the use of local culture as a tourism product, however, the cultural representation focuses on superficial values and does not bring to light the social and historical richness that the city has. Public authorities use the city marketing strategies as a means able to maximize the attractiveness of Natal urban space to investors, business groups and tourists. It can be observed that urban managers seek solutions that can continuously increase the tours, which often manifests in interventions that focus the tourist areas of the city, in oposition of those who do not contribute to a positive reading of the city, which ultimately generate the worsening of spacial and social inequalities

Comunicação pública e marketing político na gestão pública municipal

Based on the definitions of Public Communication, Political Marketing, Public Interest Information and Communication as a Tool of Governance, the study notes the use of the news section of the websites of the City Christmas and Mossoro, Rio Grande do Norte, in regard to strengthening citizenship and encouraging participatory management, from the characterization of materials like pieces of Political Marketing and Public Communication. Data collection in qualitative research was conducted from August to September 2011 and content analysis showed that the appropriation of public property for personal use is a reality, despite legal requirements to the detriment of strategic communication and governance for results from the dissemination of information of public interest and the establishment of a communication channel between State Government and Society. Elements of this study suggest the need for further research to deepen the discussion

Uma cidade para pessoas: funcionalidade, racionalidade e emotividade nas relações mobiliário urbano, espaço público e cidadãos

This research investigates how functionality, rationality, and emotionality applied to urban furniture design contribute to the organization, legibility, and qualification of public spaces, according to different layouts, physical and visual qualities of the urban furniture as well as the quality of urban infrastructure that may influence the uses and appropriation of urban spaces by pedestrians, turning those spaces into socially centripetal or centrifugal places. The work consists of two parts. First refers to a bibliographical review concerning two main issues of the investigation: product design and legibility of public space, defining the conceptual and theoretical bases, linked to methodological strategies aimed at systemic planning and integrated management of urban furniture design and legibility of public spaces (sidewalks), that are necessary to define the existing relationships among the user s interface (pedestrians); product (urban artifacts); built environment (sidewalks). The second part refers to the achievement of empirical approach throughout visual analysis of public spaces and the functional and qualitative evaluation of the existing urban furniture in the central neighborhood of Cidade Alta, Natal, RN, according to the theoretical researched concepts and methodological procedures. The results expose the weaknesses and levels of intensity arising from the relations among public space, urban furniture, and users, revealing the need for theoretical and practical approaches which would help the decision-making in planning, designing and integrating innovative, sustainable, and ethical solutions in order to design attractive public spaces that could provide sociability and citizenship throughout integrated public management, city systemic thinking and urban identity. The suggested guidelines at the end of this research will provide the necessary strategies to design suitable urban furniture systems according to aesthetic and practical urban infrastructure and city context, aiming to ease visual and physical conflicts that may negatively interfere with the appropriation of public spaces by the citizens

Avaliação diagnóstica e seu impacto na aprendizagem

Cada vez mais as escolas de governo assumem uma posição estratégica apoiando o Estado no aperfeiçoamento dos servidores públicos e dos serviços prestados por eles. A questão qualitativa que perpassa a atuação dessas escolas deve ser atentamente discutida por conta dessas implicações. Por isso, nesse cenário, buscou-se realizar um estudo de caso na Escola Nacional de Administração Pública - ENAP, escola de governo localizada em Brasília - Brasil. O objetivo geral da investigação foi verificar se a utilização da avaliação diagnóstica nos processos de avaliação dos cursos de formação e aperfeiçoamento da área de administração pública oferecidos pela Escola potencializa o grau de aprendizagem discente. Para levantar essa resposta e sua relação com o processo de ensino-aprendizagem, o público alvo escolhido foi a turma de dezembro/2015 do curso Fundamentos do Pregão Eletrônico. A partir da aplicação de uma avaliação diagnóstica junto aos alunos do curso, foi possível levantar dados importantes sobre as práticas pedagógicas e a estrutura do curso. Dos aspectos positivos, é possível ressaltar que a ênfase prática do curso confirmou-se de forma eficaz nas avaliações, até mesmo pelo perfil levantado dos alunos, contudo, um dos pontos frágeis que mais chamou a atenção durante a pesquisa foi o aspecto do cumprimento dos objetivos de aprendizagem. A avaliação diagnóstica revelou que metade da turma não se sentia apta as competências propostas pela ENAP. Ao final, foi possível confirmar a importância da avaliação diagnóstica como instrumento de avaliação e construção do conhecimento, propondo-se a sua implantação no curso Fundamentos do Pregão Eletrônico e, além de outras recomendações, a formação de uma cultura avaliativa na escola baseada na reflexão e na ação estratégica, que da mesma forma que um escultor, vai lapidando o processo educacional. O tema não finda com a obtenção e análise dos dados apresentados na pesquisa mas traz contribuições relevantes para a comunidade científica e poderá ainda, ser material importante para estudos futuros na área da excelência da Gestão Pública relacionada às escolas de governo.

Modelo para el control social a la gestión pública : el caso de las veedurías ciudadanas en el municipio de Envigado

Esta investigación parte de las diferentes iniciativas que se presentan en el escenario nacional e internacional en torno a la intención por consolidar y mejorar la función de control social a la gestión pública que ejercen por mandato constitucional las veedurías ciudadanas, a partir de referentes que orienten la labor de sus integrantes, en el caso concreto del municipio de Envigado dadas sus características y la cultura de participación ciudadana que ha implementado. En ese sentido, se indaga acerca de ¿Cómo fortalecer el control social, a través del mejoramiento en la función de seguimiento a la gestión pública que desarrollan las veedurías ciudadanas en el municipio de Envigado? En este trabajo se propone un modelo para el fortalecimiento del control social a la gestión pública que ejercen las veedurías ciudadanas, como herramienta de consulta para el desarrollo de actividades de seguimiento real y efectivo a las diferentes actuaciones que enmarcan la gestión pública; desde la formulación misma de la Política de Participación Ciudadana y su nuevo marco normativo en Colombia, el análisis e identificación de otras publicaciones que buscan facilitar la labor del veedor ciudadano con guías, instructivos, entre otros; así como un análisis de los principales obstáculos que se presentan en su función, conforme algunos referentes de investigaciones al respecto.

Transparência das informações públicas nos portais das instituições federais de educação profissional da região sul do Brasil

Considering the need of communication between public management and society and its citizens, in a way which is more effective and transparent, the aim of this research was to analyze the transparency of public information presented by the managers of the portals of southern Brazil’s Federal Institutes. It is a qualitative and quantitative research, applied as its purpose, and exploratory as its aim. In order to explain the issue from the theoretical frameworks published, it will be used as a procedure the bibliographic research and the further study field research in the six southern Brazil’s Federal Institutes. Therefore, the theoretical framework approaches aspects about public transparency, concepts of information and communication in the public sector, and it presents measurement indexes of public transparency. The data collection with the Institutes sought to measure – in the portals – the level of transparency of the information presented by the managers, applying a search tool which provided the transparency index of eleven categories of the researched items, besides verifying the presence of the required items by Access to Information Law. In the sequence, it sought to verify the Institutes managers’ perception of the aspects which composes the proposed theme, for the purpose of observing how the process of communication between public agencies and the citizen is happening. The main results obtained emphasize, in a general way, a low level of public information propagation, with a lower average of propagation to the information about social, environmental and economic responsibility, and a greater average to the information related to the institution’s general data – from its management structure to its ordinance. It was also observed that no institution which was researched fully comply with the demands of the Access of Information Law. The results about the manager perceptio demonstrate a tendency in publishing the produced information, however, elements such disorganization of information in the portals, lack of standardization of the virtual structure and type of language to use, and lack of procedures of popular participation and interaction in the portals are determiners to bring problems in the access and in the communication with the public. Besides, the short time of institutions’ creation and the short time of the managers assuming their functions must be considered as a reflex of the found results. Therewith, the present study highlighted the importance of Public Transparency theme and it reached a positive result to a scientific and social contribution.

PARTNERSHIP, SOCIOCULTURAL INFORMATION AND STIMULATION IN SMALL BRAZILIAN MUNICIPALITIES: CONCRETE EXAMPLES

The text came from the UNESP-Project in Partnership with the Public Administration: City of Echaporã ‖, a multidisciplinary project, interdepartmental and that results from a term partnership signed between the State University / Campus Marilia, the Regional Office of Articulation Planning and the Municipality of Echaporã.Given the serious social problems diagnosed in this county Administrative Region of Marilia, somaramse forces and, since April 2002, Echaporã account with the performance of a design matrix that involves the community in six (6) subprojects, among which a which emphasizes the dissemination of information (the public library as a center of information and knowledge irradiator for urban and rural areas, seeking to enter the Society daInformação). By their nature, the project-matrix is considered open and can accommodate new subprojects, where they concern the problems identified in the initial diagnosis. For its validity, each subproject has its own methodology, some innovative and will be subject to further systematization and dissemination, however, after a few months of deployment, the results show the correctness of community involvement (being representative) in all discussions and steps of research, and activities developed, widely disseminated to the target audience. The membership of the community, the leaders and the authorities can be considered a good barometer of the actions carried out in Echaporã and evidence of change in information culture that is already noticeable in the city, setting the socio-cultural dynamics of the same, in terms a new public policy to be strengthened with the participation of specialists in this specific area, in direct work with local managers, in this case, specific examples relating to the strength of information in the process of change in small municipalities.

O city marketing em Natal/RN e a construção da imagem da cidade

The growing importance of tourism in overall economic activity worldwide has favored the intensification of competition among cities that seek to create environments attractive to tourists and potential investors. It has been common practice to import characteristics of the business environment in the public management of cities. The city marketing is a key tool used by public leaders to promote a linkage between the tourism image and urban image and involves, in addition to promoting the image of the city, the planning of interventions in urban space, trying to formulate a positive image of the city able to facilitate the deployment of capital. This research seeks to understand the nature of city marketing as part of contemporary urban management and analyzes how is its application in decisions concerning the promotion of tourism in Natal/RN. The approach of this research is qualitative, exploratory and descriptive, in which respondents were the main leaders of two of the official tourism site, the Empresa Potiguar de Promoção Turística and the Secretaria Municipal de Turismo e Desenvolvimento Econômico. It was found that there is a strong articulation of public power with private enterprise in the design and conduct of the actions of urban marketing, that from the survey data show that the behavior of target markets provide guidelines for taking strategic decisions relating to tourism. Sun and sea are some key elements explored to form the image of Natal and to authorize the sale of the city as a tropical paradise. However, there is an increase in the diversification of tourism products, seeking to increase flow to the segments of ecotourism, adventure, business and culture. It s also growing the use of local culture as a tourism product, however, the cultural representation focuses on superficial values and does not bring to light the social and historical richness that the city has. Public authorities use the city marketing strategies as a means able to maximize the attractiveness of Natal urban space to investors, business groups and tourists. It can be observed that urban managers seek solutions that can continuously increase the tours, which often manifests in interventions that focus the tourist areas of the city, in oposition of those who do not contribute to a positive reading of the city, which ultimately generate the worsening of spacial and social inequalities

Comunicação pública e marketing político na gestão pública municipal

Based on the definitions of Public Communication, Political Marketing, Public Interest Information and Communication as a Tool of Governance, the study notes the use of the news section of the websites of the City Christmas and Mossoro, Rio Grande do Norte, in regard to strengthening citizenship and encouraging participatory management, from the characterization of materials like pieces of Political Marketing and Public Communication. Data collection in qualitative research was conducted from August to September 2011 and content analysis showed that the appropriation of public property for personal use is a reality, despite legal requirements to the detriment of strategic communication and governance for results from the dissemination of information of public interest and the establishment of a communication channel between State Government and Society. Elements of this study suggest the need for further research to deepen the discussion

Uma cidade para pessoas: funcionalidade, racionalidade e emotividade nas relações mobiliário urbano, espaço público e cidadãos

This research investigates how functionality, rationality, and emotionality applied to urban furniture design contribute to the organization, legibility, and qualification of public spaces, according to different layouts, physical and visual qualities of the urban furniture as well as the quality of urban infrastructure that may influence the uses and appropriation of urban spaces by pedestrians, turning those spaces into socially centripetal or centrifugal places. The work consists of two parts. First refers to a bibliographical review concerning two main issues of the investigation: product design and legibility of public space, defining the conceptual and theoretical bases, linked to methodological strategies aimed at systemic planning and integrated management of urban furniture design and legibility of public spaces (sidewalks), that are necessary to define the existing relationships among the user s interface (pedestrians); product (urban artifacts); built environment (sidewalks). The second part refers to the achievement of empirical approach throughout visual analysis of public spaces and the functional and qualitative evaluation of the existing urban furniture in the central neighborhood of Cidade Alta, Natal, RN, according to the theoretical researched concepts and methodological procedures. The results expose the weaknesses and levels of intensity arising from the relations among public space, urban furniture, and users, revealing the need for theoretical and practical approaches which would help the decision-making in planning, designing and integrating innovative, sustainable, and ethical solutions in order to design attractive public spaces that could provide sociability and citizenship throughout integrated public management, city systemic thinking and urban identity. The suggested guidelines at the end of this research will provide the necessary strategies to design suitable urban furniture systems according to aesthetic and practical urban infrastructure and city context, aiming to ease visual and physical conflicts that may negatively interfere with the appropriation of public spaces by the citizens

Um quotidiano rural no espaço urbano

Dissertação para obtenção do grau de Mestre em Arquitectura, apresentada na Universidade de Lisboa - Faculdade de Arquitetura.

Overlapping forms of knowledge in environmental governance: comparing environmental policy workers' perceptions

Public sector environmental governance involves complex interactions between different forms of knowledge. Public sector reforms have important implications for environmental governance by changing the relationships between knowledge systems. By comparing the views of environmental policy workers the implications of public sector management reform for environmental governance are explored. The analysis presented highlights that environmental policy work is contested in ways that mainstream public sector management and environmental governance literature often overlook. It is concluded that the adequacy of the conceptual frameworks informing public sector environmental reform are unclear, as are the implications of such reforms for effective environmental governance.