Staphylococcus aureus genotype B and other genotypes isolated from cow milk in European countries

Staphylococcus aureus is globally one of the most important pathogens causing contagious mastitis in cattle. Previous studies, however, have demonstrated in Swiss cows that Staph. aureus isolated from bovine intramammary infection is genetically heterogeneous, with Staph. aureus genotype B (GTB) and GTC being the most prominent genotypes. In addition, Staph. aureus GTB was found to be contagious, whereas Staph. aureus GTC and all the remaining genotypes were involved in individual cow disease. The aim of this study was to subtype strains of Staph. aureus isolated from bovine mastitic milk and bulk tank milk to obtain a unified view of the presence of bovine staphylococcal subtypes in 12 European countries. A total of 456 strains of Staph. aureus were subjected to different typing methods: ribosomal spacer PCR, detection of enterotoxin genes, and detection of gene polymorphisms (lukE, coa). Major genotypes with their variants were combined into genotypic clusters (CL). This study revealed 5 major CL representing 76% of all strains and comprised CLB, CLC, CLF, CLI, and CLR. The clusters were characterized by the same genetic properties as the Swiss isolates, demonstrating high clonality of bovine Staph. aureus. Interestingly, CLB was situated in central Europe whereas the other CL were widely disseminated. The remaining 24% of the strains comprised 41 genotypes and variants, some of which (GTAM, GTBG) were restricted to certain countries; many others, however, were observed only once.

Is "milk crust" a transient form of golden retriever ichthyosis?

BACKGROUND A recessive inherited form of lamellar ichthyosis is well recognized in golden retrievers. In this breed, young puppies demonstrate a self-limiting scaling disorder which is commonly recognized by breeders, who use the term "milk crust" to describe this syndrome. HYPOTHESIS/OBJECTIVES To determine whether "milk crust" is a new keratinization disorder or a self-limiting form of golden retriever ichthyosis. ANIMALS A total of 179 golden retriever dogs (21 dams and 158 puppies) were examined. METHODS Dermatological examination and assessment of the patatin-like phospholipase-1 (PNPLA1) genotype by PCR testing of buccal mucosal swabs. Skin biopsies from one affected puppy were evaluated for histopathological abnormalities. RESULTS Forty-five of 158 (28%) puppies exhibited scaling at 8 weeks of age; 113 of 158 (72%) were dermatologically normal. Of 144 analysed samples, 40 of 144 (28%) puppies demonstrated a homozygous mutation of the PNPLA1 genotype [of which, 36 of 40 (90%) had signs of scaling], 77 of 144 (53%) demonstrated a heterozygous mutation and 27 of 144 (19%) were a normal wild-type. In six of 17 (35%) dams, a homozygous mutation of the PNPLA1 genotype was found, eight of 17 (47%) demonstrated a heterozygous mutation and three of 17 (18%) were normal wild-type. Dams with a homozygous mutation were clinically unaffected. A 1 year follow-up revealed that 23 of 28 (82%) puppies affected with this syndrome failed to develop typical signs of ichthyosis. In five of 28 (18%) dogs there was persistence of mild scaling. CONCLUSIONS AND CLINICAL IMPORTANCE We hypothesize that the clinical syndrome termed "milk crust" could represent a transient form of golden retriever ichthyosis. Remission is not fully linked to PNPLA1 genotype, suggesting that unknown factors may contribute to the clinical disease.

A case-control study to estimate the effects of acute clinical infection with the Schmallenberg virus on milk yield, fertility and veterinary costs in Swiss dairy herds.

Schmallenberg virus (SBV) was first detected in Switzerland in July 2012 and many Swiss dairy farmers reported acute clinical signs in dairy cattle during the spread of the virus until December 2012. The objectives of the present study were to investigate the effects of an acute infection with SBV on milk yield, fertility and veterinary costs in dairy farms with clinical signs of SBV infection (case farms), and to compare those farms to a matched control group of dairy farms in which cattle did not show clinical signs of SBV infection. Herd size was significantly (p<0.001) larger in case farms (33 cows, n=77) than in control farms (25 cows, n=84). Within case herds, 14.8% (median) of the cows showed acute clinical signs. Managers from case farms indicated to have observed a higher abortion rate during the year with SBV (6.5%) than in the previous year (3.7%). Analysis of fertility parameters based on veterinary bills and data from the breeding associations showed no significant differences between case and control farms. The general veterinary costs per cow from July to December 2012 were significantly higher (p=0.02) in case (CHF 19.80; EUR 16.50) than in control farms (CHF 15.90; EUR 13.25). No differences in milk yield were found between groups, but there was a significant decrease in milk production in case farms in the second half year in 2012 compared to the same period in 2011 (p<0.001) and 2013 (p=0.009). The average daily milk yield per cow (both groups together) was +0.73kg higher (p=0.03) in the second half year 2011 and +0.52kg (p=0.12) in the second half year 2013 compared to the same half year 2012. Fifty-seven percent of the cows with acute clinical signs (n=461) were treated by a veterinarian. The average calculated loss after SBV infection for a standardized farm was CHF 1606 (EUR 1338), which can be considered as low at the national level, but the losses were subject to great fluctuations between farms, so that individual farms could have very high losses (>CHF 10,000, EUR 8333).

Pricing and Policy Problems in the Northeast Fluid Milk Industry

This article documents the need for reform of milk pricing in the Northeast. The New York price gouging law can be recast as a fair share law. This new milk policy “kills two birds with one stone.” It corrects regional inequities in raw milk pricing by reforming the pricing of milk at retail by limiting and redistributing excessive retail margins to farmers and consumers. The fair share policy relieves allocative price inefficiency, improves the performance of the federal milk market order pool, and the general performance of the Northeast dairy farming and fluid milk industries.

Psychosocial influences on fruit, vegetable and milk consumption among a cohort of Houston-area 5th graders

Epidemiological evidence suggests that fruit and vegetable intake is negatively associated with the development of several chronic diseases, including heart disease, some cancers and diabetes mellitus. Inadequate consumption of milk during developmental years is associated with osteoporosis. Consumption of fruit, vegetable and milk (FVM) declines from childhood to adolescence. Adolescent eating habits persist into adulthood; thus, understanding psychosocial factors such as self-efficacy, norms and preferences, is important for developing effective interventions. Preferences, one of the most consistent correlates of fruit and vegetable consumption in children and adolescents, may mediate the relationships between self-efficacy and norms and fruit and vegetable consumption. ^ Fifth grade students from one middle school in South Texas were followed for two years. Students completed lunch food records and questionnaires assessing fruit, vegetable and milk self-efficacy and norms and fruit and vegetable preferences. Principal component analyses identified four scales: Fruit Self-Efficacy, Vegetable Self-Efficacy, Fruit and Vegetable Norms, and Milk Influences. Reliability and validity of the four scales and emerging subscales were assessed using Cronbach's alpha and consumption data, respectively. Associations between longitudinal FVM consumption and self-efficacy and norms were tested. Additionally, the influence of preferences on the relationship of self-efficacy, norms and fruit and vegetable consumption was examined. ^ Confirmatory factor analyses confirmed four scales and subscales. Internal consistency and test-retest reliabilities were acceptable. Self-efficacy and norms were related to FVM consumption and changes in fruit and high fat vegetable consumption over the two-year period. While intake over the two-year period differed statistically, eating patterns were stable. Preferences mediated the relation between fruit self-efficacy and FV norms and fruit consumption. ^ In conclusion, self-efficacy and norms about consuming FVM at school appear to influence FVM consumption. Because eating patterns were similar over the two-year period, establishing healthy eating habits in elementary school is important. While FVM self-efficacy and norms influenced FVM consumption in children, only fruit preferences mediated the relationship of fruit consumption, self-efficacy and norms. Since the influences of FVM consumption appear to differ, interventions designed to increase consumption should target these differences and consider the specificity of self-efficacy and norms. ^

C. H. Chalmers.- Bacteriología de la leche. (Bacteria in relation to the milk supply). 271 pp., 3ª ed., 1945, reimpresa en 1949. Editado por Edward Arnol & Co., Londres

Fil: H. G. C..

Algunas reflexiones acerca de la construcción del espacio en el cuento "A Drink in the Passage" de Alan Paton : Desafíos para la traducción

El presente trabajo constituye un primer acercamiento al estudio de los cuentos comprendidos en Tales of a Troubled Land del escritor sudafricano Alan Paton (1903-1988). A modo de introducción, haremos una breve reseña histórico-social de la Sudáfrica del apartheid (Cottrell, 2005; Gibson, 2009) que vincularemos con información biográfica del escritor (Paton, 1966; Lirola, 2008). Esta información resultará, a nuestro entender, relevante al momento de hacer una lectura profunda del cuento. Luego, estudiaremos cómo se conforman los espacios interno / externo (Foucault, 1986), especialmente aquellos que posibilitan el encuentro de blancos y negros (Barnard, 2007), con el fin de explorar cómo aparecen las barreras raciales en el texto. Por último, y a la luz de lo anterior, plantearemos algunas cuestiones centrales que se constituyen en desafíos al momento de abocarnos a la tarea de traducir este cuento, especialmente las relacionadas a la metáfora cristalizada en el 'passage' que, nombrado en el título, sirve de eje estructurador de la acción. Según nuestra hipótesis, la traducción de la construcción del espacio se vuelve un desafío ya que la distribución de los espacios y la circulación de la población en y entre ellos son inherentes al apartheid, no sólo en cuanto al sistema de segregación que el término designa, o al momento histórico en el que fue implementado, sino a la (im)posibilidad de que los individuos de las diferentes razas coexistan y se interrelacionen en una tierra que (no) les es común, en un espacio construido culturalmente desde la separación en lugar de desde la unidad

Algunas reflexiones acerca de la construcción del espacio en el cuento "A Drink in the Passage" de Alan Paton : Desafíos para la traducción

El presente trabajo constituye un primer acercamiento al estudio de los cuentos comprendidos en Tales of a Troubled Land del escritor sudafricano Alan Paton (1903-1988). A modo de introducción, haremos una breve reseña histórico-social de la Sudáfrica del apartheid (Cottrell, 2005; Gibson, 2009) que vincularemos con información biográfica del escritor (Paton, 1966; Lirola, 2008). Esta información resultará, a nuestro entender, relevante al momento de hacer una lectura profunda del cuento. Luego, estudiaremos cómo se conforman los espacios interno / externo (Foucault, 1986), especialmente aquellos que posibilitan el encuentro de blancos y negros (Barnard, 2007), con el fin de explorar cómo aparecen las barreras raciales en el texto. Por último, y a la luz de lo anterior, plantearemos algunas cuestiones centrales que se constituyen en desafíos al momento de abocarnos a la tarea de traducir este cuento, especialmente las relacionadas a la metáfora cristalizada en el 'passage' que, nombrado en el título, sirve de eje estructurador de la acción. Según nuestra hipótesis, la traducción de la construcción del espacio se vuelve un desafío ya que la distribución de los espacios y la circulación de la población en y entre ellos son inherentes al apartheid, no sólo en cuanto al sistema de segregación que el término designa, o al momento histórico en el que fue implementado, sino a la (im)posibilidad de que los individuos de las diferentes razas coexistan y se interrelacionen en una tierra que (no) les es común, en un espacio construido culturalmente desde la separación en lugar de desde la unidad