Metal ions bound to the human milk immunoglobulin A: Metalloproteomic approach

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Transport of amino acids from milk whey by Caco-2 cell monolayer after hydrolytic action of gastrointestinal enzymes

The bioavailability of amino adds from milk whey protein hydrolysates was evaluated using diffusion of the substances through semi-permeable membranes (dialyzability) and transport by Caco-2 cell cultures. The hydrolysates with low degree of hydrolysis (LDH) and high degree of hydrolysis (HDH) were obtained after 120 min of reaction time at 50 degrees C after the initial addition of pepsin, followed by the addition of trypsin, chymotrypsin and carboxypeptidase-A. The proteins and hydrolysates were further subjected to in vitro digestion with pepsin plus pancreatin. HPLC was used to determine the concentrations of dialyzable amino adds (48.4% of the non-hydrolyzed proteins, 63.2% of the LDH sample and 58.3% of the HDH sample), demonstrating the greater dialyzability of the hydrolysates. The LDH and HDH whey protein hydrolysates prepared with pepsin, trypsin, chymotrypsin and carboxypeptidase-A showed only 14.7% and 20.8% of dialyzable small peptides and amino acids, respectively. The efficiency of absorption was demonstrated by the preferential transport of Ile, Lou and Arg through a layer of cells. In the LDH hydrolysate, Tyr was also transported. Prior high- and low-degree hydrolysis of the whey provided transport by 5.7% and 6.6%, respectively, in comparison with 23% for non-hydrolyzed proteins, considering the total amount of these amino adds that was applied to the cells. (C) 2014 Elsevier Ltd. All rights reserved.

Predicting breeding values for milk yield of Guzera (Bos indicus) cows using random regression models

Given the importance of Guzera breeding programs for milk production in the tropics, the objective of this study was to compare alternative random regression models for estimation of genetic parameters and prediction of breeding values. Test-day milk yields records (TDR) were collected monthly, in a maximum of 10 measurements. The database included 20,524 records of first lactation from 2816 Guzera cows. TDR data were analyzed by random regression models (RRM) considering additive genetic, permanent environmental and residual effects as random and the effects of contemporary group (CG), calving age as a covariate (linear and quadratic effects) and mean lactation curve as fixed. The genetic additive and permanent environmental effects were modeled by RRM using Wilmink, All and Schaeffer and cubic B-spline functions as well as Legendre polynomials. Residual variances were considered as heterogeneous classes, grouped differently according to the model used. Multi-trait analysis using finite-dimensional models (FDM) for testday milk records (TDR) and a single-trait model for 305-days milk yields (default) using the restricted maximum likelihood method were also carried out as further comparisons. Through the statistical criteria adopted, the best RRM was the one that used the cubic B-spline function with five random regression coefficients for the genetic additive and permanent environmental effects. However, the models using the Ali and Schaeffer function or Legendre polynomials with second and fifth order for, respectively, the additive genetic and permanent environmental effects can be adopted, as little variation was observed in the genetic parameter estimates compared to those estimated by models using the B-spline function. Therefore, due to the lower complexity in the (co)variance estimations, the model using Legendre polynomials represented the best option for the genetic evaluation of the Guzera lactation records. An increase of 3.6% in the accuracy of the estimated breeding values was verified when using RRM. The ranks of animals were very close whatever the RRM for the data set used to predict breeding values. Considering P305, results indicated only small to medium difference in the animals' ranking based on breeding values predicted by the conventional model or by RRM. Therefore, the sum of all the RRM-predicted breeding values along the lactation period (RRM305) can be used as a selection criterion for 305-day milk production. (c) 2014 Elsevier B.V. All rights reserved.

Infection of water buffalo in Rio de Janeiro Brazil with Anaplasma marginale strains also reported in cattle

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Evaluation of the effect of supplementing fermented milk with quinoa flour on probiotic activity

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Construction and preliminary characterization of a river buffalo bacterial artificial chromosome library

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Water buffalo genome characterization by the Illumina BovineHD BeadChip

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Random regression models using different functions to model milk flow in dairy cows

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Produção de leite, duração da lactação e intervalo de partos em búfalas mestiças Murrah

This study aimed to evaluate the potential for milk production (MP), lactation length (LL) and calving interval (CI), analyze the environmental component affecting these traits, and to estimate the heritability and repeatability for milk production in crossbreds of Murrah buffalo cows in the state of Alagoas, Brazil. Data was composed of 487 observations of MP from 136 lactations recorded between the years of 2000 and 2010. In the analysis of variance for PL, the fixed effects were season (1- October to March, 2 -April to September) and year of the beginning of lactation, calving order and the LL (covariate). For the analysis of LL only the fixed effect of year of the beginning of lactation was included, and finally for the CI analysis, year of the beginning of lactation and calving order. The estimates of covariance were obtained using unicharacteristic analysis by Bayesian inference method, applyingan animal model, through Gibbs sampling. The additive genetic, permanent environment and residual effects were included as random effects in the model. The averages (sd) of MP, LL and CI were 2,218.03 kg (408.18), 282.59 days (39.48) e 422.49 days (91.05), respectively. All the effects included in the models were important (P<0.01). The estimates of heritability and repeatability for PL were 0.29 and 0.69, respectively. The results suggest that there is a moderate genetic variability among individuals for PL, indicating the possibility to obtain gain using selection.

Occurrence of heat waves in Triangulo Mineiro and Alto Paranaiba and their effect on milk yield and feed intake in cattle

The aim of this study was to estimate milk production and food consumption during the occurrence of heat waves in the Triangulo Mineiro and Alto Paranaiba, MG by means of bioclimatic zoning based on the Temperature and Humidity Index (THI). Therefore a history of heat wave occurrence between the years 2000-2010 was compiled. The decline in milk production (DMP) and reduced food consumption (RFC) were simulated in cities where periods of heat waves were identified. Frutal and Ituiutaba had the highest rate of heat wave occurrence per year. The DMP and RFC showed bioclimatic differences between the cities of Uberaba, Ituiutaba and Frutal. The cities with the best bioclimatic conditions were Sacramento and Patrocinio, as they presented a THI classified outside of the emergency range, with a night THI of below 76 and without heat waves. Therefore, the occurrence of heat waves can impair food intake and decrease milk production, thereby most effectively demonstrating the effects of thermal stress on dairy cows in the Triangulo Mineiro and Alto Paranaiba, MG region.

Estudo fenotípico e genotípico da produção de biofilme por Staphylococcus aureus isolados de leite bubalino e ambiente de ordenha

Pós-graduação em Microbiologia Agropecuária - FCAV

Análise comparativa de genes das caseínas de búfalo

Pós-graduação em Genética e Melhoramento Animal - FCAV