Differential Recognition of Old World and New World Arenavirus Envelope Glycoproteins by Subtilisin Kexin Isozyme 1 (SKI-1)/Site 1 Protease (S1P).

The arenaviruses are an important family of emerging viruses that includes several causative agents of severe hemorrhagic fevers in humans that represent serious public health problems. A crucial step of the arenavirus life cycle is maturation of the envelope glycoprotein precursor (GPC) by the cellular subtilisin kexin isozyme 1 (SKI-1)/site 1 protease (S1P). Comparison of the currently known sequences of arenavirus GPCs revealed the presence of a highly conserved aromatic residue at position P7 relative to the SKI-1/S1P cleavage side in Old World and clade C New World arenaviruses but not in New World viruses of clades A and B or cellular substrates of SKI-1/S1P. Using a combination of molecular modeling and structure-function analysis, we found that residueY285 of SKI-1/S1P, distal from the catalytic triad, is implicated in the molecular recognition of the aromatic "signature residue" at P7 in the GPC of Old World Lassa virus. Using a quantitative biochemical approach, we show that Y285 of SKI-1/S1P is crucial for the efficient processing of peptides derived from Old World and clade C New World arenavirus GPCs but not of those from clade A and B New World arenavirus GPCs. The data suggest that during coevolution with their mammalian hosts, GPCs of Old World and clade C New World viruses expanded the molecular contacts with SKI-1/S1P beyond the classical four-amino-acid recognition sequences and currently occupy an extended binding pocket.

Department of Human Rights, Division of Community Action Agencies Performance Plan, FY 2009

Agency Performance Plan, Department of Human Rights, Division of Community Action Agencies

Lottery Action, Vol. 15, no. 1, January 7-27, 2008

Iowa Lottery Authority Newsletter for Lottery Retailers

Lottery Action, Vol. 15, no. 5, March 10-23, 2008

Iowa Lottery Authority Newsletter for Lottery Retailers

Lottery Action, Vol. 15, no. 6, March 21-April 8, 2008

Iowa Lottery Authority Newsletter For Lottery Retailers

Lottery Action, Vol. 15, no. 7, April 7, 2008

Iowa Lottery Authority Newsletter For Lottery Retailers

Lottery Action, Vol. 15, no. 11, June 2-15, 2008

Iowa Lottery Authority Newsletter For Lottery Retailers

Lottery Action, Vol. 15, no. 14 July 12-August 3, 2008

Iowa Lottery Authority Newsletter For Lottery Retailers

Lottery Action, Vol. 15, no. 15, August 4, 17, 2008

Iowa Lottery Authority Newsletter For Lottery Retailers

Lottery Action, Vol. 15, no. 18, August 18 - September 7, 2008

Iowa Lottery Authority Newsletter For Lottery Retailers

Lottery Action, Vol.15, no. 17, October 8-21, 2008

Iowa Lottery Authority Newsletter For Lottery Retailers

Lottery Action, Vol. 15, no. 19, October 6-19, 2008

Iowa Lottery Authority Newsletter for Lottery Retailers

Lottery Action, Vol. 15, no. 21, November 3-16, 2008

Iowa Lottery Newsletter for Lottery Retailers

2008 Affirmative Action in Iowa Annual Review

In with accordance 19B.5 of the Code of Iowa, this item has submitted the fiscal year 2008 Affirmative Action in Iowa report. The report details the progress we have made to balance our workforce in FY 2008. Just as importantly, the plan describes steps to put into practice our continued commitment to increasing diversity in the state’ workforce and details our response to the challenges we face as a result of increased talent competition. The State's renewed emphases on recruitments. along with greater oversight of state agency hiring practices, are key strategies that we must aggressively employ in competing for talent and balancing our workforce.

Iowa Division of Community Action Agencies, Strategic Plan, 2003

This report outlines the strategic plan for Iowa Division of Community Action Agenciesg including,goals and mission.