917 resultados para Thermal and mechanic cycling
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Phytoplankton productivity is the common and important factor being considered in determining the overall status of a given body of water. This is because they are found at the base of an energy or food chain, being the basic source of primary food in a given aquatic system. Hence, information on their contribution is essential in indicating how much biomass energy will be available to all other living resources in the system. Though the primary productivity of shallow lakes is characterized by mixed populations of phytoplankton and submersed aquatic vegetation in the open water. Lake Choghakhor, is a shallow lake, located in Chaharmahal-Bakhtiyari Province. This lake is the most important ecosystem in the region especially for waterfowl populations, has a recreational value and supports tourism and fisheries. During last decade Choghakhor has been influenced by some man-made impacts such as water level fluctuation, agricultural discharge and fish (Cyprinids) introduction causing a serious problem in its trophic states. So water quality for physical, chemical and biological was monitored in five sampling stations, from April 2003 to March 2004. As biological parameters we studied phytoplankton, epiphytic algae, and zooplankton and macrobenthose community structure. Chlorophyll a content for phytoplankton and epiphytes was measured to estimate production of these groups (biomass over time). Also we determined biomasses of submersed macrophytes and macrobenthose and primary production of phytoplankton (dark and light bottles technique) to estimate fish production. The results of this study showed Lake Choghakhor did not undergo stable thermal and oxygen stratification, and the lake water was mixed throughout the study (the lake mixing regime is polymictic). Now submerged plants especially Myriophyllum spicatum has covered almost the entire lake and dense macrophyte beds (Polygonom amphibium), located on the east southern end of the lake appear to act as a sink for these nutrients. Lake Choghakhor appeared to be in a macrophyte dominated clear water state with low TP (annual mean: 24± 15μg.l-1) and chlorophyll a (annual mean: 3±1.28μg.l-1) concentrations and very high Secchi depth. The grazing pressure of dominant pelagic filtering zooplankton Daphnia longespina did not seem to be significant in determining the low phytoplankton crop expressed as chlorophyll a. We expect that sequestering of nutrients by submerged plants and associated epiphytes are the dominant stabilizing mechanisms suppressing the phytoplankton crop of Lake Choghakhor.
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During its lifetime in the core, the cladding of an Accelerator Driven Subcritical Reactor (ADSR) fuel pin is expected to experience variable stresses due to frequent interruptions in the accelerator proton beam. This paper investigates the thermal fatigue damage in the cladding due to repetitive and unplanned beam interruptions under certain operational conditions. Beam trip data was obtained for four operating high power proton accelerators, among which the Spallation Neutron Source (SNS) superconducting accelerator was selected for further analysis. 9Cr-1Mo-Nb-V (T91) steel was selected as the cladding material because of its proven compatibility with proposed ADSR design concepts. The neutronic, thermal and stress analyses were performed using the PTS-ADS, a code that has been specifically developed for studying the dynamic response to beam-induced transients in accelerator driven subcritical systems. The lifetime of the fuel cladding in the core was estimated for three levels of allowed pin power and specific operating conditions. © 2012 Elsevier Ltd. All rights reserved.
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This article describes the application of the light-attenuation technique as a tool for measuring dilution occurring in buoyancy-driven flows. Whilst this technique offers the experimental fluid dynamicist the ability to make rapid synoptic buoyancy measurements non-intrusively, its successful application requires careful selection of chemical dye, dye concentration, illumination and optics. After establishing the advantages offered by methylene blue as a dyeing agent, we assess the accuracy of buoyancy measurements made using this technique compared with direct measurements made with density meters. Density measurements obtained using light-attenuation differ from those obtained using the density meter by typically less than 3%. It is hoped that this article will provide useful advice with regards to its implementation in the field of buoyancy-driven flows. © 2011 Elsevier Inc.
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We present a novel method for controlling the growth orientation of individual carbon nanotube (CNT) microstructures on a silicon wafer substrate. Our method controls the CNT forest orientation by patterning the catalyst layer used in the CNTs growth on slanted KOH edges. The overlap of catalyst area on the horizontal bottom and sloped sidewall surfaces of the KOH-etched substrate enables precise variation of the growth direction. These inclined structures can profit from the outstanding mechanical, electrical, thermal, and optical properties of CNTs and can therefore improve the performance of several MEMS devices. Inclined CNT microstructures could for instance be used as cantilever springs in probe card arrays, as tips in dip-pen lithography, and as sensing element in advanced transducers. ©2009 IEEE.
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Increasing demand for energy and continuing increase in environmental as well as financial cost of use of fossil fuels drive the need for utilization of fuels from sustainable sources for power generation. Development of fuel-flexible combustion systems is vital in enabling the use of sustainable fuels. It is also important that these sustainable combustion systems meet the strict governmental emission legislations. Biogas is considered as one of the viable sustainable fuels that can be used to power modern gas turbines: However, the change in chemical, thermal and transport properties as well as change in Wobbe index due to the variation of the fuel constituents can have a significant effect on the performance of the combustor. It is known that the fuel properties have strong influence on the dynamic flame response; however there is a lack of detailed information regarding the effect of fuel compositions on the sensitivity of the flames subjected to flow perturbations. In this study, we describe an experimental effort investigating the response of premixed biogas-air turbulent flames with varying proportions of CH4 and CO2 to velocity perturbations. The flame was stabilized using a centrally placed conical bluff body. Acoustic perturbations were imposed to the flow using loud speakers. The flame dynamics and the local heat release rate of these acoustically excited biogas flames were studied using simultaneous measurements of OH and H2CO planar laser induced fluorescence. OH* chemiluminescence along with acoustic pressure measurements were also recorded to estimate the total flame heat release modulation and the velocity fluctuations. The measurements were carried out by keeping the theoretical laminar flame speed constant while varying the bulk velocity and the fuel composition. The results indicate that the flame sensitivity to perturbations increased with increased dilution of CH4 by CO2 at low amplitude forcing, while at high amplitude forcing conditions the magnitude of the flame response was independent of dilution.
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BGCore is a software package for comprehensive computer simulation of nuclear reactor systems and their fuel cycles. The BGCore interfaces Monte Carlo particles transport code MCNP4C with a SARAF module - an independently developed code for calculating in-core fuel composition and spent fuel emissions following discharge. In BGCore system, depletion coupling methodology is based on the multi-group approach that significantly reduces computation time and allows tracking of large number of nuclides during calculations. In this study, burnup calculation capabilities of BGCore system were validated against well established and verified, computer codes for thermal and fast spectrum lattices. Very good agreement in k eigenvalue and nuclide densities prediction was observed for all cases under consideration. In addition, decay heat prediction capabilities of the BGCore system were benchmarked against the most recent edition of ANS Standard methodology for UO2 fuel decay power prediction in LWRs. It was found that the difference between ANS standard data and that predicted by the BGCore does not exceed 5%.
Resumo:
Coupled Monte Carlo depletion systems provide a versatile and an accurate tool for analyzing advanced thermal and fast reactor designs for a variety of fuel compositions and geometries. The main drawback of Monte Carlo-based systems is a long calculation time imposing significant restrictions on the complexity and amount of design-oriented calculations. This paper presents an alternative approach to interfacing the Monte Carlo and depletion modules aimed at addressing this problem. The main idea is to calculate the one-group cross sections for all relevant isotopes required by the depletion module in a separate module external to Monte Carlo calculations. Thus, the Monte Carlo module will produce the criticality and neutron spectrum only, without tallying of the individual isotope reaction rates. The onegroup cross section for all isotopes will be generated in a separate module by collapsing a universal multigroup (MG) cross-section library using the Monte Carlo calculated flux. Here, the term "universal" means that a single MG cross-section set will be applicable for all reactor systems and is independent of reactor characteristics such as a neutron spectrum; fuel composition; and fuel cell, assembly, and core geometries. This approach was originally proposed by Haeck et al. and implemented in the ALEPH code. Implementation of the proposed approach to Monte Carlo burnup interfacing was carried out through the BGCORE system. One-group cross sections generated by the BGCORE system were compared with those tallied directly by the MCNP code. Analysis of this comparison was carried out and led to the conclusion that in order to achieve the accuracy required for a reliable core and fuel cycle analysis, accounting for the background cross section (σ0) in the unresolved resonance energy region is essential. An extension of the one-group cross-section generation model was implemented and tested by tabulating and interpolating by a simplified σ0 model. A significant improvement of the one-group cross-section accuracy was demonstrated.
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This work presents simplified 242mAm-fueled nuclear battery concept design featuring direct fission products energy conversion and passive heat rejection. Optimization of the battery operating characteristics and dimensions was performed. The calculations of power conversion efficiency under thermal and nuclear design constraints showed that 5.6 W e/kg power density can be achieved, which corresponds to conversion efficiency of about 4%. A system with about 190 cm outer radius translates into 17.8 MT mass per 100 kW e. Total power scales linearly with the outer surface area of the battery through which the residual heat is rejected. Tradeoffs between the battery lifetime, mass, dimensions, power rating, and conversion efficiency are presented and discussed. The battery can be used in a wide variety of interplanetary missions with power requirements in the kW to MW range. Copyright © 2007 by the American Institute of Aeronautics and Astronautics, Inc. All rights reserved.
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The work presents simplified242mAm fueled nuclear battery concept design featuring direct fission products energy conversion and passive heat rejection. The performed calculations of power conversion efficiency under thermal and nuclear design constraints showed that 14 W/kg power density can be achieved, which corresponds to conversion efficiency of about 6%. Total power of the battery scales linearly with its surface area. 144 kW of electric power can be produced by a nuclear battery with an external radius of about 174 cm and total mass of less than 10300 kg. The mass of242m Am fuel for such a system is 3200 gram.
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In order to guarantee a sustainable supply of future energy demand without compromising the environment, some actions for a substantial reduction of CO 2 emissions are nowadays deeply analysed. One of them is the improvement of the nuclear energy use. In this framework, innovative gas-cooled reactors (both thermal and fast) seem to be very attractive from the electricity production point of view and for the potential industrial use along the high temperature processes (e.g., H 2 production by steam reforming or I-S process). This work focuses on a preliminary (and conservative) evaluation of possible advantages that a symbiotic cycle (EPR-PBMR-GCFR) could entail, with special regard to the reduction of the HLW inventory and the optimization of the exploitation of the fuel resources. The comparison between the symbiotic cycle chosen and the reference one (once-through scenario, i.e., EPR-SNF directly disposed) shows a reduction of the time needed to reach a fixed reference level from ∼170000 years to ∼1550 years (comparable with typical human times and for this reason more acceptable by the public opinion). In addition, this cycle enables to have a more efficient use of resources involved: the total electric energy produced becomes equal to ∼630 TWh/year (instead of only ∼530 TWh/year using only EPR) without consuming additional raw materials. © 2009 Barbara Vezzoni et al.
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工业革命以来,由于人口的快速增加和人类活动的强烈干扰(主要包括煤炭、石油等石化燃料的燃烧、化肥生产和使用)导致土地利用/覆被变化、大气CO2浓度升高、N沉降等一系列全球环境变化问题。有关陆地生态系统生物地球化学循环,尤其是陆地生态系统C、N循环及其耦合过程方面的研究成为全球变化科学研究领域的重要内容。 干旱/半干旱地区占地球陆地总面积的1/3。与湿润地区相比较,干旱/半干旱地区生态系统稳定性比较差,往往属于生态脆弱区。因此,全球变化对干旱/半干旱地区生态系统影响更加敏感。科尔沁沙地位于我国北方干旱/半干旱地区,是我国典型的农牧交错区和生态脆弱区。科尔沁沙地是世界上人口密度最高的干旱/半干旱地区之一,人类活动对其影响剧烈。然而,有关科尔沁沙地生态系统C、N元素生物地球化学循环过程对土地利用/覆被变化、N沉降等全球变化响应及其反馈机制的研究非常缺乏。因此,本文以科尔沁沙地退化沙质草地、农田、不同年龄樟子松和杨树人工林等生态系统为对象,开展了造林、模拟N沉降和凋落物管理对生态系统C、N元素循环过程影响的研究。 在科尔沁沙地东南缘,以退化沙质草地、樟子松(Pinus sylvestris var. mongolica)人工林(15、24和30年生)、杨树(Poplus xiaozhuanica)人工林(7、11和15年生)为对象,研究草地转变为林地对生态系统C、N储量影响;以退化草地、榆树疏林草地和32年生樟子松人工林为对象,比较草地造林对土壤C、N循环过程及其土壤微生物性状的影响;以农田和5、10、15年生杨树人工林为对象,研究退耕还林对生态系统C、N储量和循环过程影响;以35年生樟子松人工林为对象,模拟研究N沉降和凋落物管理对生态系统C、N循环过程影响。通过上述研究,得到以下主要结果: (1)草地生态系统总C储量为34.38 Mg ha-1,15、24和30年生樟子松人工林生态系统总C储量分别为43.56、60.45和66.59 Mg ha-1,7、11和15年生杨树人工林生态系统总C储量分别为34.54、48.26和78.77 Mg ha-1;与农田相比,退耕5年的杨树人工林生态系统总C库储量下降13%,而10年和15年杨树人工林分别增加了176%和5倍;随着人工林年龄的增加,地上植被生物量C库储量占生态系统总C库储量的比例逐渐增加,并主要分配在树干。草地生态系统总N库储量为2.54 Mg ha-1,15、24和30年生樟子松人工林生态系统总N库储量分别为1.96、2.10和2.19 Mg ha-1,7、11和15年生杨树人工林生态系统总N库储量分别为2.27、1.84和2.60 Mg ha-1;与农田相比,退耕5年的杨树人工林生态系统总N库储量下降32%,而10年和15年杨树人工林分别增加了47%和76%;农田和草地造林后生态系统N储量依然主要分配在土壤中。 (2)草地和农田造林后土壤C、N库储量的变化受多因子的影响,例如林龄、树种种类以及立地条件等。农田和草地造林初期,土壤C、N库储量表现出下降趋势,随着林龄的增加,土壤C、N储量逐渐恢复。草地营造樟子松人工林30年后,0–60 cm深度土壤C、N储量依然显著低于草地;与草地相比,15年生杨树人工林土壤C、N储量差异不显著。在立地条件较好的情况下,10年杨树人工林土壤C、N储量已显著高于农田;然而,在立地条件相对较差的情况下,15年杨树人工林土壤C、N储量仍然与农田相比差异不显著。 (3)土地利用变化能够强烈地改变土壤C、N循环过程。与草地或疏林草地相比,32年生樟子松人工林土壤C、N、P含量显著降低;土壤C、N矿化过程发生显著变化,并且受季节变化的影响;在不同季节,土壤微生物量碳含量、代谢熵(qCO2)、微生物熵(MBC/TOC)以及土壤酶活性等在不同土地利用条件下表现出规律不一致。同样,农田退耕杨树人工林能够显著影响土壤C、N矿化过程,土壤无机氮(铵态氮+硝态氮)含量,土壤微生物量碳含量以及土壤微生物活性。草地造林在一定程度上导致土壤质量下降。而农田造林有利于土壤质量改善,尤其在在立地条件较好情况下。 (4)N添加增加对沙地樟子松人工林地上和地下C、N元素含量影响不大;N添加1年后,仅林下植被C、N含量显著增加,高氮处理(N15)凋落物N含量显著增加。N添加抑制了沙地樟子松人工林凋落物的早期分解和N、P元素释放。5、6、8和9月份土壤无机N含量均随着N输入增加表现出一定程度的增加,然而,7月份N添加导致土壤无机N含量降低。N添加对土壤潜在N矿化速率影响不显著。7和8月份N添加影响土壤C矿化速率,而其它月份影响不显著。低氮处理(N5)有利于增加土壤微生物量碳含量,而高氮处理(N15)在一定程度上降低土壤微生物量碳含量。 (5)凋落物输入变化(凋落物添加和凋落物移出)在一定程度上改变了35年生沙地樟子松人工林生态系统C、N循环过程。凋落物移出(C0)增加了林下植被C含量,降低了树木叶片N含量。凋落物移出抑制了凋落物分解和P元素的释放,而增加了C元素的早期释放速率,对N元素释放过程影响不显著。凋落物输入变化对不同月份土壤无机N含量和土壤N矿化过程影响均不显著。仅在6月份凋落物移出显著抑制了土壤C矿化速率,其它月份差异均不显著。凋落物管理对土壤微生物量碳含量影响不显著。 以上研究结果表明,土地利用变化、N沉降和凋落物输入改变等能够影响半干旱地区沙地生态系统C、N储量和循环过程。尤其是土地利用变化强烈改变沙地生态系统C、N储量、分配格局和循环过程,并且受到多因子的影响。科尔沁沙地樟子松人工林生态系统C、N元素生物地球化学循环存在密切的耦合关系。今后有必要进一步结合3S技术、同位素技术、模型模拟以及分子生物学技术等,从微观-宏观不同尺度上,研究半干旱地区沙地生态系统C、N循环过程对全球变化的响应及其反馈机制。
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Structural relaxation by isothermal annealing below the glass transition temperature is conducted on a Zr64.13Cu15.75Ni10.12Al10 bulk metallic glass. The effect of structural relaxation on thermal and mechanical properties was investigated by differential scanning calorimetry and instrumented nanoindentation. The recovery of the enthalpy in the DSC curves indicates that thermally unstable defects were annihilated through structural relaxation. During nanoindentation, the structural relaxation did not have a significant influence on the serrated plastic flow behavior. However, Structural relaxation shows an obvious effect in increasing both the hardness and elastic modulus, which is attributed to the annihilation of thermally unstable defects that resulted from the relaxation.
Resumo:
Structural relaxation by isothermal annealing below the glass transition temperature is conducted on a Zr64.13Cu15.75Ni10.12Al10 bulk metallic glass. The effect of structural relaxation on thermal and mechanical properties was investigated by differential scanning calorimetry and instrumented nanoindentation. The recovery of the enthalpy in the DSC curves indicates that thermally unstable defects were annihilated through structural relaxation. During nanoindentation, the structural relaxation did not have a significant influence on the serrated plastic flow behavior. However, Structural relaxation shows an obvious effect in increasing both the hardness and elastic modulus, which is attributed to the annihilation of thermally unstable defects that resulted from the relaxation.
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土壤微生物量、可溶性有机碳与氮虽然只占土壤有机碳、氮总量的较小部分,但可以在土壤全碳、氮变化之前反映土壤微小的变化,又直接参与土壤生物化学转化过程,因而在植被恢复过程中,较其它土壤理化性质等能够更好地指示土壤恢复情况。在青藏高原东缘存在大面积的次生人工林替代灌丛或采伐迹地,而关于这些人工林替代后的生态效果和生态过程的评估却十分缺乏,本研究通过评估岷江上游植被恢复重建过程中典型人工替代次生植被凋落物层与土壤碳、氮等养分大小,动态监测土壤微生物生物量、水溶性碳、氮等指标,结合温度与凋落物输入等影响土壤活性有机碳、氮因子的控制试验,系统分析不同人工替代次生植被土壤碳、氮等养分的差异原因,试图寻找低效人工林优化调控与持续管理技术,为区域生态公益林持续管理提供理论和技术依据。主要结论如下: 1. 通过对不同人工替代次生植被凋落物层和土壤碳、氮分析发现,油松和华山松人工林替代次生灌丛后土壤碳、氮含量较灌丛和阔叶人工林低,主要原因可能为凋落物质量(C/N)较差,而引起碳、氮等元素难以归还土壤。进而通过对不同人工替代次生植被凋落物层和土壤微生物生物量、水溶性有机碳、氮等指标的季节性动态模式的分析,发现各次生植被土壤微生物生物量C、N,P以及土壤水溶性碳、氮含量均呈明显季节性动态,呈现秋季明显大于其它季节,冬季最低,在表层土壤最为明显。 2. 油松、华山松人工林凋落物层和土壤水溶性有机碳(WDOC)、土壤水溶性有机氮(WDON)明显低于灌丛和连香树,土壤微生物生物量C、N也以油松和华山松人工林最低,而落叶类植被,如灌丛、连香树和落叶松之间没有明显差异,说明可利用底物的数量和质量差异是影响各次生植被凋落物分解和土壤微生物活性的主要原因。MBC/OC和MBN/ON能较好地指示土壤微生物活性的变化,MBC/OC凋落层总体以灌丛和连香树人工林最高,油松和华山松人工林最低;而土壤中MBC/OC连香树人工最高,华山松人工林最低。说明以油松和华山松为主的人工造林替代乡土阔叶灌丛造成土壤C、N等养分严重匮乏,微生物活性低下是影响其养分周转的主要原因。 3. 从各次生植被凋落物产生看,凋落物年归还量最大的为华山松人工林(5.1×103 kg ha-1),其次为落叶松人工林(4.8×103 kg ha-1),阔叶灌丛林地凋落物产生总量(4.4×103 kg ha-1)略大于油松人工林(4.2×103 kg ha-1),最小的为连香树人工林(3.6×103 kg ha-1);叶是凋落物的主体,落叶类树种月动态表现为单峰型,高峰主要在10-11月,如落叶松、连香树和灌丛林;常绿的松类月动态不明显,各月基本相同,最为明显地为油松林,华山松人工林略有二个小峰,分别出现在11月和5月。落叶阔叶灌丛的凋落物分解速率大于常绿针叶林,如油松和华山松。结合凋落物的产生量和分解速率,不同树种人工林替代次生阔叶灌丛后,人工油松和华山松林枯落物总贮量和厚度明显大于落叶松人工林、灌丛林和连香树人工林,说明以油松和华山松为主的人工造林替代乡土阔叶灌丛延缓了有机物向土壤的顺利归还,不利于土壤C、N等养分循环。 4. 通过控制地面凋落物和地下根系输入有机物对土壤碳、氮的影响研究发现,(1) 单独去除根系以及根系与地面凋落物同时去除处理1年后对表层(0-10cm)土壤WDOC均没有显著影响,而土壤WDON显著增加,油松人工林土壤微生物生物量C、N显著降低,人工落叶松林没有显著差异,说明油松人工林土壤微生物活性对地下碳输入的依赖大于其它次生植被,而落叶松土壤微生物活性对地下碳输入依赖性较小;去除地面凋落物,明显降低了落叶松人工林土壤WDOC,华山松和连香树土壤WDON均较对照显著减少,油松人工林土壤微生物量C较对照显著减少;双倍增加地面凋落物处理对土壤微生物生物量、WDOC和WDON没有明显地增加,相反,连香树、华山松和油松人工林土壤WDON较对照减少。说明油松人工林微生物活性不仅依赖于地下碳输入,而且对地上有机物输入的依赖性也较大;连香树、落叶松和华山松人工林土壤微生物生物量并没有因地面凋落物的去除减少可能与土壤总有机碳含量及活性均较高有关,而双倍增加地面凋落物反而降低了土壤微生物生物量,说明凋落物覆盖后改变了土壤微气候。 5. 碳矿化累积量与有机碳含量和活性有机碳含量之间存在显著地正相关关系。凋落物碳累积矿化量、矿化速率以连香树最高,油松和华山松人工林次之,落叶阔叶灌丛低于常绿针叶纯林,导致其差异的主要原因可能为凋落物产生的时间动态模式不一样,致使凋落物起始分解时间不一致。而土壤层有机碳矿化速率和矿化量以阔叶落叶灌丛和连香树最高,油松和华山松人工土壤最低,再次证实利用针叶纯林恢复植被阻碍了有机质周转与循环。 6. 凋落物累积矿化量与C/N值呈显著地相关关系,并随着温度的升高而明显增加,而土壤累积矿化量与C/N值没有显著相关关系,说明土壤有机碳质量(C/N)对温度的响应不十分明显。通过双指数模型对不同温度下碳矿化过程进行模拟和计算出活性有机碳与惰性有机碳比例,发现温度升高促进了惰性有机碳向活性有机碳的转化,增加了活性有机碳含量,说明温度升高可促进次生植被凋落物与土壤有机质的分解,进而可影响到林地碳源/汇关系的变化。 综上,通过对不同人工替代次生植被凋落物与土壤C、N大小、以及土壤微生物生物量、水溶性C、N等指标动态变化模式研究,结合温度与凋落物数量输入等影响土壤活性C、N因子的综合分析,以油松和华山松人工纯林对山地植被恢复,延缓或阻碍了有机质周转与循环,造成了土壤肥力退化。对现有低效人工纯林改造,应为地面大量有机物分解创造条件。 Although soil microbial biomass, dissolved organic carbon (DOC) and dissolved organic nitrogen (DON) are a small part of total soil organic carbon and nitrogen, they can directly participate in the process of soil biochemical translation and indicate the fine changes before changes of soil total organic carbon and nitrogen occur. So, they are good indexes to indicate soil restoration condition during the process of vegetation rehabilitation. There are large areas of secondary vegetations which substitute for indigenous shrubs in the eastern fringe of Qinghai-Tibet Plateau. However, it is not well known that the ecological effect and process after substitution by different secondary plantations. Based on comparison of soil organic and nitrogen contents in litter layer and soil under different secondary vegetations in upper reaches of Minjiang River, soil microbial biomass, DOC and DON in litter layer and soil were investigated in order to analyze the seasonal dynamic. Combining the effects of temperature, litter input and root exclusion on soil microbial biomass, DOC and DON, we also aim to understand the reason and mechanism of difference in soil carbon and nitrogen contents among different secondary vegetations. The study would contribute to comprehensively understanding C and N cycling processes and provide optimal control and sustainable technology of low-effect plantations in these regions. The results are as follows: (1) Organic carbon and nitrogen in litter layers and soil under different substitution plantations were investigated. The results showed that contents of soil organic carbon and nitrogen were lower in P. tabulaeformis (PT) and P. armandi Franch(PA) than those in native broad-leaf shrub and broad-leaf plantation. The low quality (C/N) of litter in PT and PA plantations caused carbon and nitrogen returning to soil difficultly. Seasonal dynamic of soil microbial carbon (MBC),-nitrogen (MBN),-phosphor (MBP), and WDOC and WDON showed similar pattern, which had the highest values in autumn and the lowest values in winter. (2) WDOC and WDON in litter layers and soil under PT and PA plantations were significantly lower than those in native broad-leaf shrub and Cercidiphyllum japonicum Sieb. et Zucc.(CJ). Soil MBC and MBN were also the lowest, while there were no significant differences among deciduous vegetations, i.e. native broad-leaf shrub, CJ and Larix kaempferi Lamb.(LK) plantation. The results suggested that difference in quantity and quality of available substance was main reason that affected the activity of microbe in soil and litter layer. MBC/OC and MBN/ON were good indexes to indicate the change of soil microbial activity. MBC/OC of litter had the highest value under native broad-leaf shrub and CJ plantation, and had the lowest value in PT and PA plantations, while MBC/OC of soil was the highest under CJ plantation, and was the lowest in PT and PA plantations. These results indicated that PT and PA plantations substituting for native broad-leaf shrub caused deficit of carbon and nitrogen in soil, low microbial activity was a main reason influencing the cycling and turnover of carbon and nitrogen in soil. (3) The annual litter fall production, composition, seasonal dynamic and decomposition of five typical secondary stands in upper reaches of Minjiang River were studied in this paper. The annual litter productions were: PA (5.1×103 kg ha-1), LK(4.8×103 kg ha-1), native broad-leaf shrub (4.4×103 kg ha-1), PT(4.2×103 kg ha-1),CJ(3.6×103 kg ha-1). The litter production of leaves in five secondary vegetations occupied a higher percentage in the annual total litter production than those of other components. The litterfall was mostly producted in the cool and dry period (October-November) for deciduous vegetations and relatively equably producted in every season for evergreen coniferous vegetations. The decomposition rate of leaf litter in the broad-leaf stand was higher than those in evergreen coniferous stand. Combined with annual litter fall production and decomposition rate of leaf litter, we found that stock and depth of litter layer were significantly larger in PT and PA plantations than those in native broad-leaf shrub, LK and CJ plantations. The results confirmed that PT and PA plantations substituting for native broad-leaf shrub delayed organic matter returning to soil and hindered cycling of carbon and nitrogen again. (4) We explored plant litter removal, double litter addition, root trenching, and combining root trenching and litter removal treatments to examine the effects of above- and belowground carbon inputs on soil microbial biomass, WDOC and WDON in four secondary plantations. During the experimental period from June 2007 to July 2008, 1 year after initiation of the treatments, WDOC in soil did not vary in root trenching, and combining root trenching and litter removal treatments, while WDON in soil significantly increased compared with CK treatment. Root trenching reduced soil MBC and MBN in PT plantation, while MBC and MBN in soil did not vary in LK plantation. The rasults implied that soil microbial activity was more dependent on belowground carbon input in PT plantation than those in other secondary plantations, on the contrary, soil microbial activity in LK plantation was not dependent on belowground carbon input. Plant litter removal significantly decreased soil WDOC in LK plantation, decreased WDON in PA and CJ plantations, and also significantly reduced soil MBC in PT plantation. However, double litter addition did not increase soil microbial biomass, WDOC and WDON, on the contrary, soil WDON in CJ, PA and PT plantations were decreased. These suggested that soil microbial activity was not only dependent on belowground carbon input, but also on aboveground organic material input. Double litter addition could change the microclimate and result in the decrease of soil microbial activity in CJ, PA and PT plantations. (5) We measured carbon mineralization in a 107 days incubation experiment in 5℃,15℃ and 25℃. Carbon cumulative mineralization was positively correlated with organic matter and labile organic carbon in litter layer and soil. Cumulative carbon mineralization and mineralization rate of litter layers in PT and PA plantations were higher than that in native broad-leaf shrub. This difference between native broad-leaf shrub and coniferous plantations in cumulative carbon mineralization and mineralization rate of litter layers could be attributed to the initiating time of decomposition due to the difference in seasonal dynamic of litter fall production between two types of secondary plantations. However, cumulative carbon mineralization and mineralization rate in soil were the highest in native broad-leaf shrub and CJ plantation, and were the lowest in PT and PA plantations. This also confirmed that PT and PA plantations substituting for native broad-leaf shrub hindered the cycling and turnover of organic matter again. (6) Carbon cumulative mineralization was positively correlated with C/N in litter layer and increased with temperature increasing, while carbon cumulative mineralization was not correlated with C/N in soil. This indicated that soil organic matter quality (C/N) was insensitive to temperature. Applying bi-exponential model, we computed the percent of labile and stable carbon in different temperature incubation and found that temperature increasing would accelerate the transform from stable carbon to labile carbon and increase the percentage of labile organic carbon. This illuminated that temperature incraesing could facilitate the decomposition of litter and soil organic matter in secondary vegetations and hence affect the relationship between carbon source and sink.
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土壤线虫是土壤动物的主要功能类群之一,在土壤养分分解和循环中起到重要的作用。本研究通过施用两种形态氮肥,硝态氮(NO-3–N)和铵态氮(NH+4–N),对黄瓜整个生长期内根际土壤线虫的群落组成、结构及其多样性等的影响进行了比较研究。为增进土壤健康,提高土壤质量以及合理施用氮肥提供科学的理论依据。研究结果如下: 1. 氮肥处理后,不同浓度NO-3–N处理提高了根际土壤线虫数量,而NH+4–N处理(202.5 kg N/ hm2)抑制了线虫数量的增加。线虫群落结构相对较稳定,营养类群变化不大。且少量优势科/属会对土壤线虫群落特征起着至关重要的作用。 2. 在整个生长季节内,非寄生线虫的群体动态变化与寄生线虫的群体的动态变化具有相反的变化趋势。其中,NO-3–N处理和NH+4–N处理后植物寄生线虫出现频率的变化趋势相近,都是由高到低;非植物寄生线虫出现频率的变化趋势则都是由低到高。这说明非植物寄生线虫数量增长和空间占位对植物寄生线虫群体有一定的抑制作用。另外,也反映了适量氮肥在一定程度上能够减轻植物寄生线虫对黄瓜的危害。 3. 由多样性指数变化可知,NO-3–N和NH+4–N在低肥(67.5 kg N/hm2)和高肥(202.5 kg N/hm2)处理较中肥(135.0 kg N/hm2)处理,更不利于提高土壤线虫多样性地提高和线虫群落的稳定性。中肥不同形态氮肥处理与对照相比,H´指数在初花期和结果期显著增加了土壤线虫的H´指数,说明施入两种形态的氮肥能够提高线虫生物多样性程度。NH+4–N处理在初花期和结果期显著降低了土壤线虫种类的丰富性。土壤线虫生物多样性变化中,H´和SR指数在一定程度上能够反映施用无机氮肥对土壤线虫的多样性的影响,而J指数和λ指数效果不明显。NO-3–N和NH+4–N处理相比较,NO-3–N处理对黄瓜土壤线虫的多样性指数影响更大,促进了土壤线虫群体的多样化和种类的丰富度,更有利于提高土壤线虫的多样性,增加其稳定性。这些结果表明适量无机氮肥特别是NO-3–N的施用对黄瓜土壤线虫的生物多样性有一定的维护和提高作用。 4. 线虫数量与土壤质量指标的相关分析表明:线虫数量与有关土壤理化生指标,如土壤NO-3–N、NH+4–N、有机质含量等的正相关程度高,与总酚含量等显著负相关;与根际土壤微生物,细菌、真菌、放线菌数量等呈显著正相关。另外,线虫数量与土壤含水量未表现出显著相关关系。 Nematodes play a major role in decomposition and nutrient cycling in soil. Nematode community analyses are useful in assessing soil ecosystem status and function. The effects of two forms of mineral nitrogenous fertilizers (NO-3–N and NH+4–N) on nematode community composition, structure and diversity in rhizosphere of cucumber were investigated during different growing seasons of cucumber. Systematically research of effects of nitrogenous fertilizers could help to obtain better undstanding of a healthy soil and using nitrogenous fertilizers in reason. The main results are as follows: 1. The total numbers of nematode were more abundant in NH+4–N treatments than other teatments. However, NH+4–N teatment(202.5 kg N/hm2)dramatically inhibited it. All the tropic groups in the soil nematode communities were stable, and the dominant family or genus had an important function in the nematode community structure. 2. There was similar trend of the frequency of plant parasitic nematodes between NO-3–N and NH+4–N treatment, the similar trend of the frequency of non-plant parasitic nematodes was also found. But the frequency of plant parasitic nematodes exhibited a contrary trend to that of plant parasitic nematodes after different nitrogenous fertilizer treatments. The results showed that the increasing trend of the frequency and the niche of non-plant parasitic nematodes inhibited the plant parasitic nematodes, and indicated that right chemical fertilizers dosage could abate plant parasitic nematodes harm to cucumber. 3. The changes of the biodiversity index showed that the nitrogen treatment(135.0 kg N/hm2)promoted the stabilization of soil nematode diversity than other nitrogen treatments(67.5 kg N/hm2 and 202.5 kg N/hm2). In the treatment(135.0 kg N/hm2),The changes in nematode diversity between the control plots and treated plots were compared by the biodiversity index (H´, J, SR, λ). Among these tested index, H´ and SR were effective in reflecting the effects of different nitrogenous fertilizers on the diversity of soil nematodes. In comparison with the NH+4–N treatment, the NO-3–N treatment promoted the stabilization of soil nematode diversity. 4. Correlation coefficients between nematode abundance and soil quality indices indicated that the total numbers of nematode were affected positively by NO-3–N, NH+4–N and the organic matter, and negatively by total phenolic acids; the total num- bers of nematode had positive correlation with bacteria, fungi and actinomycetes nu- mbers. Soil water contents had only a weak negative influence on it.