Seleção de micro-organismos com potencial de biodegradação de hidrocarbonetos e biodiesel

The oil and biodiesel are oily substances which, if spilled or thrown out in the environment, can cause serious damages. In this context, bioremediation techniques can be used to the recovering of sites degradated by mineral or vegetable oil, besides them, the biodegradation. However, it is necessary that there are microorganism strains used to degradate the contaminant, previously selected for the fast, efficient and minimum impact remediation. The main objective of this study was to isolate microorganism strains coming from different ecological recesses and verify their potentiality on biodegradating oil and biodiesel. Strains of bacteria, fungi and yeasts were isolated from soils contaminated by hydrocarbons (diesel, gasoline, grease) before, by doing striations on culture plates. On the biodegradation tests, it was used the redox indicator, 2,6-dichlorophenol indophenol (DCPIP), then, after the indicador’s discoloration, the absorbance of the suspension obtained was measured. 46 different microorganism strains were isolated, and the oil, when it was used as a carbon resource, it was degradate easier than the biodiesel. The experiments showed that, in a period, the site impacted by oil spills recovers itself by the biodegradation, in aerobic conditions, by the redox activity.

Caracterização estrutural da Zona de Falha da Cachoeira

Este trabalho apresenta a Caracterização Estrutural da Zona de Falha da Cachoeira na região compreendida entre as cidades de Jundiaí e Monte Mor no estado de São Paulo. A Zona da Falha da Cachoeira constitui um importante alinhamento estrutural de direção NW no Embasamento Cristalino e adentra-se no mesmo sentido na Bacia do Paraná afetando os sedimentos paleozóicos. Para a investigação da Zona de Falha foi necessária a definição das características geológico-estruturais e tectônicas da área. As análises macro e mesoscópicas indicam forte estruturação de relevo e dos alinhamentos de drenagem para direção NW-SE e subordinadamente para NE-SW. A zona de falha é marcada pela presença de rochas com texturas miloniticas, cataclásticas e trama de cristais isorientados não deformados. Falhas normais, transcorrentes dextrais, sinistrais foram identificadas e suas análises permitiram inferir um quadro evolutivo para a zona da Falha da Cachoeira, onde as falhas de direções NW-SE antigas relacionadas à paleotectônica e a formação das rochas miloniticas e cataclásticas , as NE-SW aos falhamentos normais da tectônica distensiva do sistema rifts do Sudeste Brasileiro que geraram os depósitos terciários e as falhas normais NW-SE a aos movimentos transcorrentes neotectônicos.

Análise das respostas de atributos sísmicos dos carbonatos barremiano, albiano e neógeno no campo de Bonito, bacia de Campos - RJ

The Bonito oil field, located on southwest of Campos Basin-RJ, has been explored since 1982. The main reservoir is composed by calcarenites of Quissamã Formation (Macaé Group) from Albian, but two other carbonate levels are present on the field, firsts is Coqueiros Formation (Aptian) and the second Siri Member (Oligo-Miocene). In this context and considering that carbonates reservoirs are a challenge for exploratory geoscientists, since the difficulty on recognize the effective reservoir distribution. This work aim to characterize the geophysical/geological facies based on seismic attributes responses, related to reservoir geometrical distribution, for the tree carbonates intervals on Bonito oil Field. A tree dimensional interpretation of the levels has been developed, based on well cross correlation and a 3D seismic interpretation, resulting on the stratigraphic and structural framework of the field, which showed a NE-SW fault trend controlling the Aptian carbonates reservoirs, and halocnetics structures showing a structural trap on Albian carbonates reservoirs. The definition of the structural/ stratigraphic framework possibly the seismic attributes calculations over the reservoir intervals. To select the best response in comparison with the reservoir distribution, obtained by seismic interpretation, the attributes response were compared with isopachs maps of each carbonate stratigraphic level. The attributes Maximum Amplitude, Maximum Magnitude and Rms Amplitude showed a good answer to reservoir distribution. The Rms Amplitude also showed a good correlation with physical rock properties, like RHOB bulk density, for the Albian and Aptian carbonates, as consequence it is possible make a characterization of reservoir distribution based on seismic attribute answer

Avaliação do efeito ambiental nas propriedades mecânicas e viscoelásticas de compósitos híbridos de titânio/fibra de carbono/resina epóxi

Hybrid composites combining metal plates and laminates with continuous fiber reinforced polymer, called fiber-metal (CHMF), have been particularly attractive for aerospace applications, due mainly to their high mechanical strength and stiffness associated with low density. These laminates (CHMF) consist of a sandwich structure consisting of layers of polymer composites and metal plates, stacked alternately. This setting allows you to combine the best mechanical performance of polymer composites reinforced with long fibers, to the high toughness of metals. Environmental effects should always be considered in the design of structural components, because these materials in applications are submitted to the effects of moisture in the atmosphere, the large cyclical variations of temperature around 82 ° C to -56 ° C, and high effort mechanical. The specimens of fibermetal composite were prepared at EMBRAER with titanium plates and laminates of carbon fiber/epoxy resin. This study aims to evaluate the effect of different environmental conditions (water immersion, hygrothermal chamber and thermal shock) of laminate hybrid titanium/carbon fiber/epoxy resin. The effects of conditioning were evaluated by interlaminar shear tests - ILSS, tensile, and vibration free

Caracterização estrutural da mineralização da mina de Fazenda Nova - GO

The study area is includes in the geological context of Arenópolis Magmatic Arc, a region where there are neoproterozoic associations of granodioritic and tonalitic composiotion. (Ortogneisses of the western Goiás) and sequences metavolcanic-sedimentary (Jaupaci Metavolcanic-sedimentary Sequence ). In the mapped area, both units are covered by a cover-laterite. The Ortogneisses from Goiás West consist of a source granodioritic gneisses, corresponding to the Biotite granodiorite gneisse, and also by tonalitic gneiss composition corresponding to Metatonalit. The Jaupaci Metavolcanic-sedimentary Sequence is formed by Chlorite Schist (Metabasalt), Biotite Schist (Metadacite) and Sericite Schist (Metarhyolite), and even intrusions Sin/Tardi e Post Tectonic, granite to diorite composition (Diorites), and alson tonalitic (Bacilandia Tonalite). Post tectonic intrusions are observed, wich were Hornblend Diorite Porphyry and Lamprophyres, Structural analysis allowed the identification of three deformational events, Dn-1, Dn and Post-Dn. The first event is associated with a bygone foliation, lineation which generates an intersectional event, generating the foliation Sn, this being the most important structure in the study area, generating even the type mineral lineation and stretch. The last deformational event is characterized by folds on different scales, affecting the Sn foliation. The rocks of the region have features s active hydrothermal and regional metamorphism, and are composed os assembly indicative of mineralogical facies metamorphism Green Schist, in chlotite zone, with evidence of retro metamorphism. Locally there are sulfides as pyrite, arsenopyrit and pyrhotite, and te mineralization is associated with the arsenopyrite

Evolução tectonossedimentar dos principais reservatórios de hidrocarbonetos do Campo de Golfinho (Bacia do Espírito Santo)

The Golfinho Field is located in the offshore region of the Espírito Santo Basin. Its importance is linked to the current average production about 19,000 barrels of oil per day, in turbidite reservoirs , giving it the nineteenth placement among the largest oil fields producing of Brazil. By interpretation and correlation’s methods based on 2D seismic sections and geophysical well logs, the study of tectonic-sedimentary evolution of major Golfinho Field’s reservoirs, which are located in Maastrichtian , aims understand and characterize the geological model of the area for the purpose of identify the main structures and types of reservoirs, improving the geological understanding of the area and using this knowledge at similar sets, that may present exploratory success in similar cases. By structural contour maps and geological cross-sections generated since time-depth conversion , the results defined for the geological model of the area , two distinct tectonic styles: a distensinal tectonics style , characterized by grabens and horsts , which belongs to rift phase, and a salt tectonics style, characterized by salt domes , listric faults and folds rolllover folds type, which belongs to marine phase . The interpretation of seismic sections and subsequent analysis of the main deformations present in the Maastrichtian reservoirs rocks ( turbidites ) showed that the northern region of the field is the most affected by salt tectonics . As for reservoirs, it was concluded to be associated to tectonics formed by rollover folds type, being older than listric faults

Influência da luz LED em culturas de células-tronco mesenquimais in-vitro

Stem cells are defined as cells capable of self-renewal and differentiation into specialized cells when submited to external signalings in the enviroment. Among adult stem cells, mesenchymal cells occupy an important position because they can differentiate into mesodermal cells such as osteoblasts, adipocytes and chondrocytes. Cell therapy consists in the use of mesenchymal stem cells (MSC) in the treatment of degenerative diseases and harmed tissue reconstruction. Due to the longstanding and costly procedure for cultivation of MSC, it was proposed the use of low power light sources, such as light emitting diodes (LED), to optimize these factors. Recent works have shown a series of results from the influence of LED light on biological tissues such as increased rate of cell proliferation, increased RNA, DNA and ATP synthesis rate. The purpose of this study is to compare the biomodulator effect of LED light set at wavelengths 630nm ± 10nm and 805nm ± 10nm on the mesenchymal stem cells proliferation. For this, the mesenchymal stem cells culture adopted the procedure used in the Departament of Animal Reproduction and Veterinary Radiology of the Faculty of Veterinary Medicine and Animal Sciences of Botucatu. MSC were obtained from an adult horse bone marrow, and isolated by density gradient separation, with the FICOLL reagent and by centrifugation. The pellet containing the stem cells was removed and these were placed in low glucose DMEM culture medium, containing 10% fetal calf serum and antibiotics. The material was observed daily by inverted microscopy for monitoring the progression of the cells and subsequently the amount of cells were counted in a Neubauer counting chamber. The amount of MSC was obtained by cell culture seeded in 24 wells culture plate and segregated into three distinct groups: Group 1 was irradiated with wavelength set at 630nm ± 10 nm, Group... (Complete abstract click electronic access below)

Flexão e análise dinâmico-mecânica de compósitos 5HS e NCF com resina epóxi monocomponente produzidos por RTM

In order to study resin distribution and homogeneity of composite laminates manufactured by RTM, it was used CYCOM 890 monolithic toughened epoxy as a matrix with two different configurations of intermediated modulus (IM) carbon fibers: Satin Weave (5HS) and non crimp fabric (NCF). The injection parameters were defined based on Thermo Gravimetric Analysis (TG), Differential Scanning Calorimetry (DSC) and rheological analysis. After processing the material, the resin/fiber impregnation was studied using ultrasonic test, Thermo Gravimetric Analysis, Differential Scanning Calorimetry, Dynamic Mechanical Analysis (DMA) and flexural tests. Therefore, it was able to observe an internal residual stress during the cooling process in both laminates, higher in the composite using NCF fabric due to the lack of symmetry, although a good proportion of fiber/matrix has been verified by the lower values of flexural modulus deviation. The DMA enabled the visualization of glass transition and its association with the inter and intra molecular interaction and movement, in which the NCF composite presented better permeability due to the lowest temperature of glass transition, when compared to the Satin Weave composite

Efeito de atmosfera controlada no desenvolvimento de Monilinia fructicola e na qualidade pós-colheita de pêssegos 'Aurora-1'

Pós-graduação em Agronomia (Produção Vegetal) - FCAV

Chauliocheilos saxatilis, a new genus and species of Hypoptopomatinae from rio Jequitinhonha basin, with a unique labial appendix (Teleostei: Loricariidae)

Chauliocheilos saxatilis, new genus and species, is described from headwater streams of the rio Itamarandiba, upper rio Jequitinhonha basin, southeastern Brazil. The new genus is diagnosed from all other Loricariidae members by having a unique labial appendix at laterodorsal portion of lower lip, associated to the proximal region of insertion of the maxillary barbel. From all Hypoptopomatinae genera, Chauliocheilos can be distinguished by having two additional series of lateral plates, one between the dorsal and mid-dorsal series, and the other between the mid-ventral and ventral series. In addition, the great number of lateral plates, the absence of the iris operculum, and the posterior dorsal-fin insertion contacting the neural spine of eighth vertebra, are other useful features to recognize the genus. The phylogenetic position of Chauliocheilos among the Hypoptopomatinae, as well as the morphology and possible functions of the labial appendix, are discussed.

Distribuição espacial dos ninhos de Athene cunicularia (coruja-buraqueira) e dinâmica de sua utilização

A coruja-buraqueira (Athene cunicularia) nidififica no chão e, com frequência, ocorre em ambientes bastante urbanizados como no campus universitário da UNESP em Botucatu, SP região centro-oeste paulista. Os objetivos do estudo foram descrever as características físicas das escavações, distribuição espacial, formas de utilização e avaliar que elementos presentes nas áreas urbanas são importantes para a seleção dos sítios de nidificação. Foram monitorados 6 grupos familiares desde 2011, cuja população e o número de ninhos foram contabilizados e a localização, georeferenciados. Os dados comportamentais foram registrados em caderno de campo, fotografados e filmados. A densidade populacional no câmpus foi de 0,03 indivíduos por Km² com os sítios de nidificação vizinhos tendo a distância mínima de 140,0 metros e máxima de 378 metros. As tocas apresentaram 50,6± 5,6cm de diâmetro, a maioria construída em terreno com declive. As corujas selecionaram sítios de nidificação em áreas abertas e gramadas contendo no entorno poleiros naturais (árvores, arvoretas, arbustos) e artificiais (placas, postes, peitoral de janelas, etc) e próximos a bueiros e postes com iluminação artificial. Estes dois últimos constituíram atrativos paras as suas presas (artrópodes e roedores) e a análise de ergagrópilas revelou a ocorrência de 5 grupos taxonômicos de artrópodes: 65,4% de Coleóptera, 27,2% de Orthoptera, 4,6% de Blatodea, 1,4% de Aranae, 0,9% de Hymenoptra e 0,4% de Mantodea. Os resultados obtidos mostraram que as corujas-buraqueiras levaram em consideração características como oferta abundante de alimento, principalmente, de artrópodes que se concentravam nas área iluminadas e nos bueiros. A estabilidade temporal dos sítios de nidificação nestes três anos de monitoramento acompanhada de sucesso reprodutivo de 50% revela que a despeito do intenso tráfego de veículos e transeuntes no campus, o ambiente ...

Crescimento de apatita na superfície da liga Ti-25Ta empregando dois tipos de tratamento de superfície: biomimético e deposição de polímero por eletrofiação

Titanium and its alloys has been widely used as materials for metallic biomaterials implants are usually employed to restore the hard tissue function, being used for artificial joints and bones, synthetic plates, crowns, dental implants and screws . Objective of this work was the surface modification of Ti-alloy 25Ta from biomimetic surface treatment of employment and deposition of polymer by electrospinning. The league was obtained from the fusion of the pure elements in the arc furnace with controlled atmosphere. The ingots were subjected to heat treatment, cold forged and sectioned discs with 13 mm diameter and 3 mm thick. Two surface treatments was evaluated, biomimetic and electrospinning with PCL fiber. The biomimetic treatment was performed involving alkaline treatment for three molarities 1.5M, 3M and 5M with immersion in SBF. The electrospinning was performed using PCL polymer alloy surface after the alkali treatment Ti25Ta 1M. For this group the polymer coated surfaces were immersed in calcium phosphate containing solution for immobilization of apatite. The results were compared with previous studies using surface treatment group to verify hydroxyapatite formation on the sample surface and it is concluded that the best condition is biomimetic treatment with 5M alkali treatment and heat treatment at 80 ° C for 72 hours

Biocompatibilidade da terapia fotodinâmica: estudo in vitro e in vivo

Pós-graduação em Ciência Odontólogica - FOA

Análise morfotectônica do Planalto Ocidental Paulista, ao sul do Rio Tietê: indicadores de deformações neotectônicas na fisiografia da paisagem

Pós-graduação em Geologia Regional - IGCE

Análise comparativa da ação antimicrobiana dos cimentos endodônticos Acroseal, Sealapex e AH plus pós presa em biofilme de enterococcus faecalis

Pós-graduação em Ciência Odontólogica - FOA