Dichlorvos exposure impedes extraction and amplification of DNA from insects in museum collections

Background: The insecticides dichlorvos, paradichlorobenzene and naphthalene have been commonly used to eradicate pest insects from natural history collections. However, it is not known how these chemicals affect the DNA of the specimens in the collections. We thus tested the effect of dichlorvos, paradichlorobenzene and naphthalene on DNA of insects (Musca domestica) by extracting and amplifying DNA from specimens exposed to insecticides in two different concentrations over increasing time intervals. Results: The results clearly show that dichlorvos impedes both extraction and amplification of mitochondrial and nuclear DNA after relatively short time, whereas paradichlorobenzene and naphthalene do not. Conclusion: Collections treated with paradichlorobenzene and naphthalene, are better preserved concerning DNA, than those treated with dichlorvos. Non toxic pest control methods should, however, be preferred due to physical damage of specimens and putative health risks by chemicals.

Robert Cornell, Technical Editor, Electronic Technician Magazine

New York Trade School graduate Robert Cornell is pictured at his desk in his position of Technical Editor, Electronic Technician Magazine. Original caption reads "Robert Cornell - Advanced Television 1954, was appointed Technical Editor of Electronic Technician Magazine in 1956 and has served with distinction since. He is President of CETA (Certified Electronic Technicians Association), which consists of alumni of the New York Trade School who are graduates of the Advanced Television Course. He was recently invited to serve a a member of the Attorney General's Committee seeking to establish safeguards to protect the public in the field of television servicing." Black and white photograph with caption to glued to reverse.