915 resultados para RNA INTERFERENCE
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Historically, the ichthyofauna of large Brazilian rivers has been subject to anthropogenic interference, such as impoundments. Currently, cage fish farming systems are a new source of impact on aquatic ecosystems. The objective of this study was to characterise the impact of freshwater fish farms on the feeding of five species of Neotropical freshwater fish. Specimens of Astyanax altiparanae, Galeocharax knerii, Iheringicthys labrosus, Pimelodus maculatus and Plagioscion squamosissimus were sampled in areas around two systems of cage fish farming (CF), and two control areas (CT) that were not influenced by this activity. Results show that there were significant changes in the diet of trophic generalist species (A. altiparanae, P. maculatus and I. labrosus) accompanied by a related increase in the condition factor values of these species in cage areas. Trophic specialist species, such as the carnivorous fish species G. knerii and P. squamosissimus, presented small differences between the CF and CT areas with regard to diet and showed no differences in other analyses performed. In conclusion, cage fish farms can affect the natural diet of trophic generalist fish species, directly affecting the nutritional status (condition factor), where food wastes was found to be one of the principal items consumed by this trophic guild. Results indicate that these species are responsible for recycling a great quantity of organic matter transferred by this type of activity, which, along with local fishery activities, contribute to mitigation of associated processes of eutrophication. © 2013 Elsevier B.V.
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Pós-graduação em Biotecnologia - IQ
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Pós-graduação em Biociências e Biotecnologia Aplicadas à Farmácia - FCFAR
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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The increased demand for using the Industrial, Scientific and Medical (ISM) unlicensed frequency spectrum has caused interference problems and lack of resource availability for wireless networks. Cognitive radio (CR) have emerged as an alternative to reduce interference and intelligently use the spectrum. Several protocols were proposed aiming to mitigate these problems, but most have not been implemented in real devices. This work presents an architecture for Intelligent Sensing for Cognitive Radios (ISCRa), and a spectrum decision model (SDM) based on Artificial Neural Networks (ANN), which uses as input a database with local spectrum behavior and a database with primary users information. For comparison, a spectrum decision model based on AHP, which employs advanced techniques in its spectrum decision method was implemented. Another spectrum decision model that considers only a physical parameter for channel classification was also implemented. Spectrum decision models evaluated, as well as ISCRa's architecture were developed in GNU-Radio framework and implemented on real nodes. Evaluation of SDMs considered metrics of: delivery rate, latency (Round Trip Time - RTT) and handoff. Experiments on real nodes showed that ISCRa architecture with ANN based SDM increased packet delivery rate and presented fewer frequency variation (handoff) while maintaining latency. Considering higher bandwidth as application's Quality of Service requirement, ANN-SDM obtained the best results when compared to other SDM for cognitive radio networks (CRN).
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Pós-graduação em Ciências Biológicas (Microbiologia Aplicada) - IBRC
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Pós-graduação em Patologia - FMB
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Pós-graduação em Biotecnologia - IQ
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Pós-graduação em Microbiologia - IBILCE
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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The vaccinal antibodies interference represents one of the problems in the leptospirosis diagnostic on serum. The present study aimed to determine the pattern of serum agglutinins anti-Leptospirae spp in vaccinated female buffaloes against leptospirosis using two types of commercial vaccines: bacterin and extern membrane. The temporal interference of vaccinal titers on serum diagnostic was evaluated. Three groups of 11 adult female buffaloes were established as follows: G1 control, non-vaccinated; G2: vaccinated with bacterin containing six serovars and G3 with extern membrane vaccine containing five serovars. A booster was administered at 30 days from the first vaccination (dfv) and two re- vaccinations were performed in each semester (days 210 and 390). Serum samples were collected on days 0, 15, 30, 45 and 60 and every 30 days until 540 dfv, being submitted to Serum Agglutination Microscopy (SAM) against the serovars present in the vaccine. G1 remained always negative. Both vaccines induced serologic responses when assessed by SAM at 150 days post first vaccination against all serovars and they revealed maximum titers around days 45 and 60 after first vaccination. At the re-vaccination there was an increase on agglutinin levels, but of less intensity than the levels previously observed. After six months from the second revaccination (540 dfv), they were almost zero, which demonstrates the short duration of diagnostic interference. The serologic monitoring of the vaccinated herds can be an efficient method to evaluate the status of protection provided by the vaccine.