Reproductive Strategies of Brazilian Lizards of the Genus Tropidurus Rodrigues, 1987 (Squamata, Tropiduridae) in the Temporal and Spatial

Reproductive strategy is the set of adaptations that promote the most efficient way that the species will survive under the particular conditions of a determined environment. Understanding these adaptations is important and can help pinpoint populations indicator of environmental changes. Spermatogenesis is a measurable biological process of these adaptations in spatial and temporal scales. We analyzed the morphology of the testes and oviducts of the lizard species that comprise the genus Tropidurus, taking into account the geographical distribution and sympatric relations. For the analysis and the testes were removed from the middle part of the oviducts from Tropidurus etheridge, T oreadicus, T itambere, T spinulosus and T Guarani species, collected in different places in the Mato Grosso state, Brazil. The reproductive period is synchronous for males and females and occurs in September, October and November. Reproductive males were characterized. In the testes are seminiferous tubules with germ cells at different stages of spermatogenesis, with a high epithelium, at present light, free spermatozoa in the lumen and reduction of interstitial tissue. For females, the reproduction peak occurs when the oviduct epithelium is high with secretions and basal nucleus. These months are characterized in the sampled areas over a period of heavy rain and high temperatures. The decline of reproductive period was observed in both sexes, between April and August. Low reproduction in males is characterized by ample light, absence of sperm, only germ cells in the early stages of spermatogenesis are observed (a few spermatogonia and spermatocytes) and interstitial tissue wide. In females, the period of reproductive decline is marked by the absence of unicellular glands in the oviduct epithelium, with higher affinity with the dye. This period corresponds to low rainfall periods and lower temperatures. We propose an analysis of zoological samples; this is a proposal to facilitate the work of many researchers through access to the species, especially rare species.

Seasonal testicular changes in Dendropsophus minutus Peters, 1872 (Anura, Hylidae)

The reproductive cycle in anurans may be either continuous or discontinuous. These differences may be connected to seasonal climate changes and/or to anthropic activity. Forty adult male individuals of the Dendropsophus minutus species were collected during one year, in the municipality of Chapada dos Guimaraes (Mato Grosso, Brazil). The testicles were studied under light and transmission electron microscopy. No variations were observed when the diameter of the seminiferous tubules and the thickness of the interstitial tissue were studied. However, changes in spermatogenesis were conspicuous and indicated that the reproductive cycle of D. minutus in Chapada dos Guimaraes is discontinuous and seems related to variations in air temperature and rainfall.

Understorey fire propagation and tree mortality on adjacent areas to an Amazonian deforestation fire

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

The Rondonian-San Ignacio Province in the SW Amazonian Craton: An overview

The Rondonian-San Ignacio Province (1.56-1.30 Ga) is a composite orogen created through successive accretion of arcs, ocean basin closure and final oblique microcontinent-continent collision. The effects of the collision are well preserved mostly in the Paragua Terrane (Bolivia and Mato Grosso regions) and in the Alto Guapore Belt and the Rio Negro-Juruena Province (Rondonia region), considering that the province was affected by later collision-related deformation and metamorphism during the Sunsas Orogeny (1.25-1.00 Ga). The Rondonian-San Ignacio Province comprises: (1) the Jauru Terrane (1.78-1.42 Ga) that hosts Paleoproterozoic basement (1.78-1.72 Ga), and the Cachoeirinha (1.56-1.52 Ga) and the Santa Helena (1.48-1.42 Ga) accretionary orogens, both developed in an Andean-type magmatic arc; (2) the Paragua Terrane (1.74-1.32 Ga) that hosts pre-San Ignacio units (>1640 Ma: Chiquitania Gneiss Complex, San Ignacio Schist Group and Lomas Manechis Granulitic Complex) and the Pensamiento Granitoid Complex (1.37-1.34 Ga) developed in an Andean-type magmatic arc; (3) the Rio Alegre Terrane (1.51-1.38 Ga) that includes units generated in a mid-ocean ridge and an intra-oceanic magmatic arc environments; and (4) the Alto Guapore Belt (<1.42-1.34 Ga) that hosts units developed in passive marginal basin and intra-oceanic arc settings. The collisional stage (1.34-1.32 Ga) is characterized by deformation, high-grade metamorphism, and partial melting during the metamorphic peak, which affected primarily the Chiquitania Gneiss Complex and Lomas Manechis Granulitic Complex in the Paragua Terrane, and the Colorado Complex and the Nova Mamore Metamorphic Suite in the Alto Guapore Belt. The Paragua Block is here considered as a crustal fragment probably displaced from its Rio Negro-Juruena crustal counterpart between 1.50 and 1.40 Ga. This period is characterized by extensive A-type and intra-plate granite magmatism represented by the Rio Crespo Intrusive Suite (ca. 1.50 Ga), Santo Antonio Intrusive Suite (1.40-1.36 Ga), and the Teotonio Intrusive Suite (1.38 Ga). Magmatism of these types also occur at the end of the Rondonian-San Ignacio Orogeny, and are represented by the Alto Candeias Intrusive Suite (1.34-1.36 Ga), and the Sao Lourenco-Caripunas Intrusive Suite (1.31-1.30 Ga). The cratonization of the province occurred between 1.30 and 1.25 Ga. (C) 2009 Elsevier Ltd. All rights reserved.

Assessment of tropical forest degradation by selective logging and fire using Landsat imagery

Many studies have assessed the process of forest degradation in the Brazilian Amazon using remote sensing approaches to estimate the extent and impact by selective logging and forest fires on tropical rain forest. However, only a few have estimated the combined impacts of those anthropogenic activities. We conducted a detailed analysis of selective logging and forest fire impacts on natural forests in the southern Brazilian Amazon state of Mato Grosso, one of the key logging centers in the country. To achieve this goal a 13-year series of annual Landsat images (1992-2004) was used to test different remote sensing techniques for measuring the extent of selective logging and forest fires, and to estimate their impact and interaction with other land use types occurring in the study region. Forest canopy regeneration following these disturbances was also assessed. Field measurements and visual observations were conducted to validate remote sensing techniques. Our results indicated that the Modified Soil Adjusted Vegetation Index aerosol free (MSAVI(af)) is a reliable estimator of fractional coverage under both clear sky and under smoky conditions in this study region. During the period of analysis, selective logging was responsible for disturbing the largest proportion (31%) of natural forest in the study area, immediately followed by deforestation (29%). Altogether, forest disturbances by selective logging and forest fires affected approximately 40% of the study site area. Once disturbed by selective logging activities, forests became more susceptible to fire in the study site. However, our results showed that fires may also occur in undisturbed forests. This indicates that there are further factors that may increase forest fire susceptibility in the study area. Those factors need to be better understood. Although selective logging affected the largest amount of natural forest in the study period, 35% and 28% of the observed losses of forest canopy cover were due to forest fire and selective logging combined and to forest fire only, respectively. Moreover, forest areas degraded by selective logging and forest fire is an addition to outright deforestation estimates and has yet to be accounted for by land use and land cover change assessments in tropical regions. Assuming that this observed trend of land use and land cover conversion continues, we predict that there will be no undisturbed forests remaining by 2011 in this study site. Finally, we estimated that 70% of the total forest area disturbed by logging and fire had sufficiently recovered to become undetectable using satellite data in 2004. (C) 2010 Elsevier B.V. All rights reserved.

Groundwater geochemical evolution in the northern portion of the Guarani Aquifer System (Brazil) and its relationship to diagenetic features

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Othonella araguaiana (Bivalvia, Megadesmidae) from the Corumbatai Formation (Midlle [Middle] Permian), Eastern Margin of the Parana Basin: systematic, evolutionary and biostratigraphic significances.

In this study, the occurrence of Othonella araguaiana Mendes, a rare bivalve species is reported for the first time in the Pinzonella illusa biozone, Middle Permian Corumbatai Formation, in the State of São Paulo. This species was originally described in coeval rocks of the Estrada Nova Formation (= Corumbatai) from the Alto Araguaia and Alto Garcas regions, State of Mato Grosso. The specimens of O. araguaiana were found in the base of a bioclastic sandstone bed, a proximal tempestite, in the middle of the Corumbatai Formation, in the city of Rio Claro, São Paulo State. The silicified shells and internal molds are well preserved, showing impressions of muscle scars and other internal anatomic characters (e.g., hinge), never illustrated by previous authors. In his original description, Mendes (1963) called attention to the similarity between O. araguaiana and Terraia aequilateralis, a common veneroid of the Corumbatai Formation. Conversely, Runnegar and Newell (1971) suggested that O. araguaiana belongs to Megadesmidae, being a junior synonym of Plesiocyprinella carinata (the commonest megadesmid of the Passa Dois Group). Our study indicates that O. araguaiana is indeed a megadesmid, but is distinct from the P. carinata. The new occurrence of O. araguaiana demonstrates that a) the paleobiogeographic distribution of this species is wider than previously thought (that it was restricted to the northern part of Parana Basin, Mato Grosso State); b) the molluscan fauna of the Corumbatai Formation (P. illusa biozone) in the State of São Paulo is more diverse and dominated by megadesmids; and c) the composition of the molluscan fauna of the Corumbatai Formation in Alto GarYas, State of Mato Grosso, is essentially the same as that of the P. illusa biozone of the eastern margin of the Parana Basin.

Malignant catarrhal fever-like lesions associated with ovine herpesvirus-2 infection in young calves (Bos indicus): a case report

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Manejo de irrigação e nitrogênio no feijoeiro comum cultivado em sistema plantio direto

With technological innovations, such as irrigation, the bean has been grown by producers who have the most varied levels of technology that, in suitable times, allows the planting great success in grain yield. The aim of this study was to evaluate the response of the dry bean to different managements of irrigation and nitrogen fertilization with no-tillage system, in Aquidauana - MS, Brazil. The experiment was conducted at the Universidade Estadual de Mato Grosso do Sul (UEMS), with the soil of the area classified as Alfisol, using the bean crop "Perola" sown on June 30, 2007. The experimental design was a randomized block split-plot consisting of three blocks and two replications within each block. The plots were composed of three management of irrigation, by the Class A pan method, using Hargreaves-Samani equation, and management by tensiometry (-40 kPa), with water replacement of 16.5 mm for all irrigation plots. The subplots consisted on four rates of nitrogen fertilization (0; 50; 100 and 150 kg ha(-1)), in which the nitrogen source was urea. It was concluded that the irrigation management through the Class A pan and Hargreaves-Samani equation conduced to higher grain yields of bean, 3031.11 and 3005.02 kg ha(-1) respectively.

Doses de potássio em cobertura na primeira soca da cultura da cana-de-açúcar cultivada no norte matogrossense

The sugar cane crop has been cultivated in all regions of the Brazil, occupying different soil types. The north region of Mato Grosso has favorable climate conditions for the culture, however, has been achieved low productivity due to the not application of fertilizer and inefficient doses. Being the potassium the mineral element absorbed in large amount by culture. Aimed to with this work to evaluate the effect of growing doses of the nutrient applied in coverage in the second cycle of the variety of sugar cane IAC86-2480. The experimental design was randomized blocks, with four replications. The Potassium doses were: 0; 60; 120; 180 e 240 kg ha(-1) of K(2)O. Was evaluated the degrees Brix, diameter, weigth, height and productivity of stalk. No was significant effects for the variable degrees Brix in response to potassium doses, since to others had response quadratic. The dose of maximum agronomic efficiency was of 150 kg ha(-1) de K(2)O obtaining productivity of 85 t ha(-1) of stalk, representing 25% increase in productivity.

Prevalence and molecular characterization of Anaplasmataceae agents in free-ranging Brazilian marsh deer (Blastocerus dichotomus)

Anaplasmataceae organisms comprise a group of obligate intracellular gram-negative, tick-borne bacteria that can infect both animals and humans. In the present work we investigate the presence of Ehrlichia, Anaplasrna, and Neorickettsia species in blood samples from Brazilian marsh deer (Blastocerus dichotomus), using both molecular and serologic techniques. Blood was collected from 143 deer captured along floodplains of the Parana River, near the Porto Primavera hydroelectric power plant. Before and after flooding, marsh deer were captured for a wide range research program under the financial support of São Paulo State Energy Company (CESP), between 1998 and 2001. Samples were divided into four groups according to time and location of capture and named MS01 (n = 99), MS02 (n = 18) (Mato Grosso do Sul, before and after flooding, respectively), PX (n = 9; Peixe River, after flooding), and AGUA (n = 17; Aguapei River, after flooding). The seroprevalences for Ehrlichia chaffeensis and Anaplasma phagocytophilum were 76.76% and 20.2% in MS01, 88.88% and 5.55% in MS02, 88.88% and 22.22% in PX, and 94.12% and 5.88% in AGUA, respectively. Sixty-one animals (42.65% of the total population) were PCR-positive for E. chaffeensis PCR (100.0% identity based on 16S rRNA, dsb, and groESL genes). Seventy deer (48.95% of the total population) were PCR-positive for Anaplasma spp. (99.0% of identity with A. platys, and in the same clade as A. phagocytophilum, A. bovis, and A. platys based on 16S rRNA phylogenetic analysis). Our results demonstrate that Brazilian marsh deer are exposed to E. chaffeensis and Anaplasma spp. and may act as reservoirs for these rickettsial agents, playing a role in disease transmission to humans and other animals. (c) 2012 Elsevier Ltd. All rights reserved.

Doses e fontes alternativas de nitrogênio no milho sob plantio direto em solo arenoso

The objective of this work was to evaluate the effect of nitrogen sources and rates on maize nutrition and grain yield under no-tillage system in sandy soil. The experiment was conducted in a Typic Quartzipsamment, in the city of Cassilandia, State of Mato Grosso do Sul, Brazil. A completely randomized block design was used in a factorial array 3x4 with four replicates. Treatments consisted of three sources [urea, extruded urea with starch (Starea), and ammonium sulfonitrate with nitrification inhibitor of 3,4-dimethylpyrazole-phosphate (Entec (R) 26)] and four rates (0, 45, 90 and 180 kg ha(-1)) of nitrogen, applied in side-dressing when the plants presented four and six expanded leaves. The nitrogen source affected N, K, and S concentration in the leaf, but did not interfere in the components of production and maize grain yield. Nitrogen application like ammonium sulfonitrate promoted higher concentrations of N, K and S in the maize leaf in relation to the urea, especially in the larger rates studied. Side-dressing nitrogen fertilization increased the number of ears per plant, number of grains per ear and grain yield, grown under no-tillage system in sandy soil, independent of the used source.

Prevalence and risk factors for human toxoplasmosis in a rural community

Toxoplasma gondii infection may lead to important pathological questions, especially in rural areas, where several sources of infection exist. Therefore, it is important to determine risk factors in order to establish adequate prophylactic measures. The present study aimed to assess the prevalence and risk factors involved in human toxoplasmosis infection in a rural community, in Eldorado, Mato Grosso do Sul State, Brazil. This community was composed of 185 farms - with 671 inhabitants - from which 20 were randomly chosen. In these farms, blood samples were collected from rural workers, who also answered a risk factor questionnaire. Serum samples were analyzed by means of direct agglutination test for the detection of anti-Toxoplasma gondii antibodies. From 73 samples collected, 79.45% were positive. None of the studied variables was significantly associated with the prevalence of the infection. However, among the individuals who reported eyesight impairments, 94.4% had anti-T. gondii antibodies, compared with 74.0% who did not report eyesight changes (p = 0.0594). Moreover, most individuals in the study (68.20%) were older than 18 years and presented 84.44% positivity, compared with 66.67% of positive individuals younger than 18 years old. We were able to conclude that a high prevalence of antibodies did not imply significant associations with the risk factors studied.

TESA-blot for the diagnosis of Chagas disease in dogs from co-endemic regions for Trypanosoma cruzi, Trypanosoma evansi and Leishmania chagasi

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

A GEP-ISFG collaborative study on the optimization of an X-STR decaplex: data on 15 Iberian and Latin American populations

In a collaborative work carried out by the Spanish and Portuguese ISFG Working Group (GEP-ISFG), a polymerase chain reaction multiplex was optimized in order to type ten X-chromosome short tandem repeats (STRs) in a single reaction, including: DXS8378, DXS9902, DXS7132, DXS9898, DXS6809, DXS6789, DXS7133, GATA172D05, GATA31E08, and DXS7423. Using this X-decaplex, each 17 of the participating laboratories typed a population sample of approximately 200 unrelated individuals (100 males and 100 females). In this work, we report the allele frequencies for the ten X-STRs in 15 samples from Argentina (Buenos Aires, CA(3)rdoba, Rio Negro, Entre Rios, and Misiones), Brazil (SA o pound Paulo, Rio de Janeiro, Parana, and Mato Grosso do Sul), Colombia (Antioquia), Costa Rica, Portugal (Northern and Central regions), and Spain (Galicia and Cantabria). Gene diversities were calculated for the ten markers in each population and all values were above 56%. The average diversity per locus varied between 66%, for DXS7133, and 82%, for DXS6809. For this set of STRs, a high discrimination power was obtained in all populations, both in males (a parts per thousand yen1 in 5 A- 10(5)) and females (a parts per thousand yen1 in 3 A- 10(9)), as well as high mean exclusion chance in father/daughter duos (a parts per thousand yen99.953%) and in father/mother/daughter trios (a parts per thousand yen99.999%). Genetic distance analysis showed no significant differences between northern and central Portugal or between the two Spanish samples from Galicia and Cantabria. Inside Brazil, significant differences were found between Rio de Janeiro and the other three populations, as well as between SA o pound Paulo and Parana. For the five Argentinean samples, significant distances were only observed when comparing Misiones with Entre Rios and with Rio Negro, the only two samples that do not differ significantly from Costa Rica. Antioquia differed from all other samples, except the one from Rio Negro.