The relationship between otolith increments and time for yellowfin and skipjack tuna marked with tetracycline

ENGLISH: The rate at which increments are deposited on the sagittal otoliths of yellowfin (Thunnus albacares) and skipjack (Katsuwonus p elamis) tunas is determined by a markrecapture experiment using tetracycline. During growth in fork length from 40 to 110 em, and for a period of up to 389 days, yellowfin of the Revillagigedo Islands- Baja California region deposit one increment per day in either the postrostrum or rostrum position of the otolith. For skipjack of the same region, rostrum increments underestimate time by approximately 24 percent during growth from 42 to 64 cm and over the maximum interval of 249 days. The growth rate of each species is estimated from the recapture fork length and the linear change in an otolith dimension following tetracycline injection. Over specific ranges in fork length the rates are 3.06 and 1.15 em per month for yellowfin and skipjack, respectively. SPANISH: La rapidez (tasa) en la que se depositan los incrementos en los otolitos sagitales del aleta amarilla (Thunnus albacares) y el barrilete (Katsuwonus pelamis) se determina mediante un experimento al recapturar los peces que han sido marcados con tetraciclina. Durante el crecimiento de la longitud de horquilla de 40 a 110 cm y por un período hasta de 389 días, se forma en el aleta amarilla de la región de las Islas Revillagigedo-Baja California, un incremento diario ya sea en el parte del postrostrum o rostrum de los otolitos. Con respecto al barrilete de la misma region los incrementos en el rostrum subestiman aproximadamente el tiempo en un 24 por ciento durante el crecimiento de 42 a 64 cm y sobre un intervalo máximo de 249 días. El índice de crecimiento de cada especie se estima en la recaptura según la longitud de horquilla y el cambio lineal en la dimensión de un otolito después de la inyección de tetraciclina. La variación específica sobre la longitud de horquilla de los índices son 3.06 y 1.15 cm por mes para el aleta amarilla y el barrilete, respectivamente. (PDF contains 54 pages.)

A study of the length at maturity and hooks/gill net mesh selection in Clarias lazera from Lake Chad

The minimum length at first maturity of Clarias lazera was found to be 24 cm (4.8%) for females and 20 cm (1.8%) for males. Fifty percent maturity was attained at length of 28 cm to 30 cm for both sexes; there being little difference among the sexes at this level of maturity. The modal retention lengths for gill nets were: 13 cm for 25.5 mm mesh; 18 cm for 32 mm mesh; 28 cm for 57 mm mesh; and 38 cm for 76 mm mesh. Modal lengths of Clarias lazera caught by various hooks sizes were No. 10 (28 cm); No. 11 (33 cm); Nos. 15 and 16 (28 cm). It is recommended that to protect the clarias fishery in Lake Chad, the use of gill nets of less than 57 mm mesh size and fishing hook No. 16 (and smaller sizes) which caught 43.94% of immature fishes should be discouraged

Panama City Fisheries Resources Office: FY 2005 Annual Report

HIGHLIGHTS FOR FY 2005 1. Assisted with a study to assess hurricane impacts to Gulf sturgeon critical foraging habitat. 2. Documented Gulf sturgeon marine movement and habitat use in the Gulf of Mexico. 3. Documented Gulf sturgeon spawning with the collection of fertilized eggs in the Apalachicola River, Florida. 4. Documented Gulf sturgeon spawning with the collection of fertilized eggs in the Yellow River, Florida. 5. Assisted with benthic invertebrate survey at Gulf sturgeon marine foraging grounds. 6. Implemented Gulf Striped Bass Restoration Plan by coordinating the 22nd Annual Morone Workshop, leading the technical committee, transporting broodfish, and coordinating the stocking on the Apalachicola-Chattahoochee-Flint (ACF) river system. 7. Over 87,000 Phase II Gulf striped bass were marked with sequential coded wire tags and stocked in the Apalachicola River. Post-stocking evaluations were conducted at 45 sites in the fall and spring and 8 thermal refuges in the summer. 8. Completed fishery surveys on 4 ponds on Eglin AFB totaling 53 acres, and completed a report with recommendations for future recreational fishery needs. 9. Completed final report for aquatic monitoring at Eglin AFB from 1999 to 2004. 10. Completed a field collection of the endangered Okaloosa darter to be incorporated into a status review to be completed in FY06. 11. Provided technical assistance to the Region 4 National Wildlife Refuge (NWR) program on changes to the fishery conservation targets for the region. Also provided technical assistance to four NWRs (i.e., Okefenokee NWR, Banks Lake NWR, St. Vincent NWR, and St. Marks NWR) relative to hurricanes and recreational fishing. 12. A draft mussel sampling protocol was tested in wadeable streams in Northwest Florida and southwest Georgia, and an associated field guide, poster, and Freshwater Mussel Survey Protocol and Identification workshop were completed in FY05. 13. Implemented recovery plan and candidate conservation actions for 14 listed and candidate freshwater mussels in the Northeast Gulf Watersheds. 14. Initiated or completed multiple stream restoration and watershed management projects. A total of 7.5 stream miles were restored for stream fishes, and 11 miles of coastline were enhanced for sea turtle lighting. A total of 630 acres of wetlands and 2,401 acres of understory habitat were restored. 15. Conducted a watershed assessment to develop a threats analysis for prioritizing restoration, protection, and enhancement to natural resources of Spring Creek, Georgia and Canoe Creek, Florida. 16. Continued the formation of an Unpaved Road Interagency Team of Federal, State, and local agencies in Northwest Florida to promote stream protection and restoration from unpaved road sediment runoff. Began the development of a technical committee agreement. 17. Conducted Alabama Unpaved Road Inventory within the Northeast Gulf Ecosystem. Data collection will be completed during FY06. 18. Finalized the development of two North Florida hydrophysiographic regional curves for use by the Florida Department of Transportation (DOT) and others involved with stream restoration and protection. Initiated the development of the Alabama Coastal Plain Riparian Reference Reach and Regional Curves for use by the Alabama Department of Environmental Management (ADEM). 19. Provided technical assistance in collecting data, analysis, and thesis formulation with Troy University, Alabama, to identify the influence of large woody debris in southeastern coastal plain streams. 20. Completed pre- and post-restoration fish community monitoring at several restoration projects including Big Escambia Creek, Magnolia Creek, and Oyster Lake, Florida. 21. Established a watershed partnership for the Chipola River in Alabama and Florida and expanded development and participation in the Spring Creek Watershed Partnership, Georgia. 22. Continued to identify barriers which inhibit the movement of aquatic species within the Northeast Gulf Ecoregion. 23. Completed a report on road crossing structures in Okaloosa darter streams to guide the closure/repair/maintenance of roads to contribute to recovery of the endangered species. In cooperation with Three Rivers RC&D Council, fish passage sites identified in the report were prioritized for restoration. 24. Monitored Aquatic Nuisance Species in the Apalachicola River and tested the sterility of exotic grass carp. 25. Multiple outreach projects were completed to detail aquatic resources conservation needs and opportunities. Participated in National Fishing Week event, several festivals, and school outreach.

Design and selection tools for sports equipment

This report presents work from the first nine month of a project investigating design methodologies and selection tools to promote innovations in sports equipment. Particular consideration is given to product design improvements and new market adoption of advanced materials and processes. Our aim is to couple appropriately similar technologies so as to provide a method of transfer between sports equipment designs. We would like to make barriers between isolated sports equipment markets more transparent without releasing proprietary information. A brief history of sports equipment design is included; issues particularly relevant to material and process technologies are outlined for sports equipment. A start has been made on a software program to express most of this information in a concise and accessible format. The methodology is reviewed with some industrial case studies. There is a need for further research to extend and address the design issues raised in this document; a suggested research programme is attached.

Molecular genetics of the Drosophila eyes absent gene

The Drosophila compound eye has provided a genetic approach to understanding the specification of cell fates during differentiation. The eye is made up of some 750 repeated units or ommatidia, arranged in a lattice. The cellular composition of each ommatidium is identical. The arrangement of the lattice and the specification of cell fates in each ommatidium are thought to occur in development through cellular interactions with the local environment. Many mutations have been studied that disrupt the proper patterning and cell fating in the eye. The eyes absent (eya) mutation, the subject of this thesis, was chosen because of its eyeless phenotype. In eya mutants, eye progenitor cells undergo programmed cell death before the onset of patterning has occurred. The molecular genetic analysis of the gene is presented.

The eye arises from the larval eye-antennal imaginal disc. During the third larval instar, a wave of differentiation progresses across the disc, marked by a furrow. Anterior to the furrow, proliferating cells are found in apparent disarray. Posterior to the furrow, clusters of differentiating cells can be discerned, that correspond to the ommatidia of the adult eye. Analysis of an allelic series of eya mutants in comparison to wild type revealed the presence of a selection point: a wave of programmed cell death that normally precedes the furrow. In eya mutants, an excessive number of eye progenitor cells die at this selection point, suggesting the eya gene influences the distribution of cells between fates of death and differentiation.

In addition to its role in the eye, the eya gene has an embryonic function. The eye function is autonomous to the eye progenitor cells. Molecular maps of the eye and embryonic phenotypes are different. Therefore, the function of eya in the eye can be treated independently of the embryonic function. Cloning of the gene reveals two cDNA's that are identical except for the use of an alternatively-spliced 5' exon. The predicted protein products differ only at the N-termini. Sequence analysis shows these two proteins to be the first of their kind to be isolated. Trangenic studies using the two cDNA's show that either gene product is able to rescue the eye phenotype of eya mutants.

The eya gene exhibits interallelic complementation. This interaction is an example of an "allelic position effect": an interaction that depends on the relative position in the genome of the two alleles, which is thought to be mediated by chromosomal pairing. The interaction at eya is essentially identical to a phenomenon known as transvection, which is an allelic position effect that is sensitive to certain kinds of chromosomal rearrangements. A current model for the mechanism of transvection is the trans action of gene regulatory regions. The eya locus is particularly well suited for the study of transvection because the mutant phenotypes can be quantified by scoring the size of the eye.

The molecular genetic analysis of eya provides a system for uncovering mechanisms underlying differentiation, developmentally regulated programmed cell death, and gene regulation.

Selection of efficient hanging ratios of gillnet on fish catch in Lake Kainji, as means of alleviating poverty among artisanal fishermen in Nigeria

Five experimental gillnet each measuring 50mx 3m nylon multi filament netting of 3" by210/2 mesh size were constructed using 40%, 45%, 50%, 55% and 60% hanging percentages, the report was carried out at Yunawa fishing village on the eastern bank of Lake Kainji. The nets were set over night (6 hours approximately). Between April-July 2004, the fish caught by the five nets were recorded taking into consideration the three mode of capture i.e. enmeshing entanglement and wedging Weight number and percentage mean weight and number based on species at five different hanging ratios were analyzed in general 50% hanging ratio was found to be the best followed by 40% among others. There was significant difference (P<0.05) in the mode of capture for both hanging ratios. Most of the fish were caught by entanglement i.e. about 83% of the catch was by entanglement while 505 hanging ratio was the best considered after the report. The occurrence of species of the five hanging ratios has significant difference (P<0.05) in terms of catch by weight and number

Comparative study on the catch efficiency and size selection of entangling nets in Lake Kainji, Nigeria

The relative catch performance and selectively of gillnets and trammel nets were investigated in 12 sampling stations in Lake Kainji, Nigeria. 3 types of nets with dimensions 50mx3m were constructed using 76mm and 178mm meshsizes for two gillnets, 76mm and 178mm meshsizes for the lint and ar mour nets of the trammelnets respectively. All the nets were randomly ganged together to form a fleet of nine nets each, and were set twice in each of the 12 stations which gave a total of 24 fishing operations. A total of 365 fish weighing 88.9kg and belonging to 16 different species were caught in all the nets. The trammelnet had the highest catch by number and weight constituting 60% and 69.22% of the total catch and weight respectively with a relative species Diversity Index of 0.82. This was followed by 76mm gillnet which constituted 38.63% by number, 28.09% by weight, 0.69 relative Species Diversity Index. The 178mm gillnet had the least catch of 1.37% and 2.9% by number and weight respectively with 0.25 relative Species Diversity Index. There was significant difference (P<0.05) in the number and weight of fish caught in the different nets. The minimum selection length for these species caught were the same for each net. The trammel net had a wider selection range that skewed to the right, a higher modal and median length indicating larger individual species being entangled in the net