Viabilidade e longevidade de sementes de tabebuia aurea benth. & hook. submetidas a diferentes métodos de armazenamento

Seeds Tabebuia aurea (syn. Handroanthus aureus Mattos), The seeds of Tabebuia aurea (syn. Handroanthus Mattos aureus), have low metabolic rates, which favors their storage conditions and freezing temperatures when stored at room temperature, there is a progressive decline in viability. Therefore, this study aims to determine the viability and longevity of seeds of Tabebuia aurea subjected to different storage methods. There were used 6100 seeds. In 2400 seeds were stored refrigerator, which in 1200 placed in transparent plastic bag and 1200 in Kraft paper bags. 3600 Other seeds were placed in room temperature and 1200 seeds in clear plastic bag, 1200 in Kraft paper bags and tray open in 1200. For each treatment, four replicates of 25 seeds for 12 months, with design was completely randomized. The average germination, normal seedlings and speed germination index were transformed into (x+1)(-1/2). The data were submitted to ANOVA and means compared by Tukey test at 5%. Also regressions polynomials were modeled for the variables study in function of the periods of storage of the seeds. The seeds stored in the refrigerator at 13 C maintained a high germination rate and normal seedling germination rate during the 360 days of evaluation. The method suitable for the seed storage of Tabebuia aurea is in the refrigerator at 13 degrees C, both in plastic and paper.

A diet enriched in linoleic acid compromises the cryotolerance of embryos from superovulated beef heifers

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Neurotoxicity of neem commercial formulation (Azadirachta indica A. Juss) in adult zebrafish (Danio rerio)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Improved sperm cryosurvival in diluents containing amides versus glycerol in the Przewalski's horse (Equus ferus przewalskii)

Two studies were conducted to understand sperm cryosensitivity in an endangered equid, the Przewalski's horse (Equus ferns przewalski), while testing the cryoprotectant ability of formamides. The first assessed the toxicity of permeating cryoprotectants (glycerol, methylformamide IMF] and dimethylformamide [DMF]) to Przewalski's horse spermatozoa during liquid storage at 4 C. The second examined the comparative influence of three diluents (with or without formamides) on cryosurvival of sperm from the Przewalski's versus domestic horse. When Przewalski's horse spermatozoa were incubated at 4 C in INRA 96 with differing concentrations of glycerol, MF or DMF or a combination of these amides, cells tolerated all but the highest concentration (10% v/v) of MF alone or in combination with DMF, both of which decreased (P < 0.05) motility traits. There was no effect of cryoprotectants on sperm acrosomal integrity. In the cryosurvival study, average sperm motility and proportion of cells with intact acrosomes in fresh ejaculates were similar (P> 0.05) between the Przewalski's (67%, 84%, respectively) and domestic (66%, 76%) horse donors. Sperm from both species were diluted in lactose-EDTA-glycerol (EQ), Botu-Crio (BOTU; a proprietary product containing glycerol and MF) or SM (INRA 96 plus 2% [v/v] egg yolk and 2.5% [v/v] MF and DMF) and then frozen over liquid nitrogen vapor. After thawing, the highest values recovered for total and progressive sperm motility, acrosomal integrity and mitochondria] membrane potential were 42.4%, 21.8%, 88.7% and 25.4 CN (CN = mean JC-1 fluorescence intensity/cell on a channel number scale), respectively, in the Przewalski's and 49.3%, 24.6%, 88.9% and 25.8 CN, respectively, in the domestic horse. Although sperm progressive motility and acrosome integrity did not differ (P> 0.05) among treatments across species, mitochondrial membrane potential was higher (P< 0.05) in both species using EQ compared to BOTU or SM media. Additionally, Przewalski's stallion sperm expressed higher (P < 0.05) post-thaw total motility in BOTU and SM compared to EQ whereas there were no differences among freezing diluents in the domestic horse. In summary, Przewalski's stallion sperm benefit from exposure to either MF or DMF as an alternative cryoprotectant to glycerol. Overt sperm quality appears similar between the Przewalski's and domestic horse, although the total motility of cells from the former appears more sensitive to certain freezing diluents. Nonetheless, post-thaw motility and acrosomal integrity values for Przewalski's horse spermatozoa mimic findings in the domestic horse in the presence of INRA 96 supplemented with 2% (v/v) egg yolk and a combined 2.5% concentration of MF and DMF. Published by Elsevier Inc.

Death-associated odors induce stress in zebrafish

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Toward an Understanding of the Growth of Ag Filaments on alpha-Ag2WO4 and Their Photoluminescent Properties: A Combined Experimental and Theoretical Study

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Chemometric optimization of solid-liquid extraction with low-temperature partition (SLE-LTP) for determination of persistent organic pollutants in Caiman yacare eggs

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Caracterização, criopreservação e diferenciação in vitro de células tronco, derivadas de amniótica e gelatina de Wharton canina, visando a formação de um banco de células tronco

Pós-graduação em Biotecnologia Animal - FMVZ

Sources and levels of selenium on breast meat quality of broilers

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Uso de invertase imobilizada em pó de sabugo de milho para produção de açúcar invertido

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Ocorrência de poluentes orgânicos persistentes em ovos de Caiman yacare (jacaré-do-Pantanal)

Pós-graduação em Química - IQ

Transplante de medula óssea para suporte de células tronco hematopoiéticas em cães acometidos por linfoma

Lymphoma represents the most prevalent hematopoietic malignancy in small animal medicine. It is highly responsible to chemotherapy and therefore several protocols are used as therapeutic tools. For that reason, the bone marrow transplantation, enshrined in human medicine through initial trial in canine patients, has increasingly become the focus of studies in order to make it a reality also in veterinary medicine. First, the treatment with the chosen chemotherapy protocol is made. As complete remission of lymphoma is observed, it must be initiated the bone marrow harvesting. The obtained material is subjected to the processes of erythrocyte depletion, plasma depletion, cryoprotectants addition, total nuclear cells counting, hematopoietic progenitor quantification, analysis of cell viability and freezing. Following that, with radiotherapy or application of cyclophosphamide, the conditioning phase of the patient who is receiving the transplantation is carried out. The bags containing hematopoietic stem cells are then thawed and transplanted into the receptor organism. Support with hematopoietic stem cells allows the use of lethal doses of chemotherapy or radiotherapy and has been shown to considerably raise the disease remission time and survival rate of the canine patients

Avaliação do estado emocional associado à catalepsia causada pela administração intraperitoneal de haloperidol

Haloperidol is a dopamine receptor antagonist used to treat schizophrenia. When systemically administered in rodents, haloperidol induces catalepsy, a state of immobility very similar to that seen in Parkinson's disease. It is known that many of Parkinson's disease symptoms are dependent on the emotional state since patients are still able to respond to external triggers such as loud noise or visual signaling. Recent data highlighted the importance of glutamatergic neurotransmission in the inferior colliculus (IC) on the cataleptic state induced by haloperidol in rats. Given the importance of IC in the brain aversion system and its connections to motor pathways, and based on the clinical reports of the emotional influence on the motor aspect of Parkinson's disease, the objective of the present study was to evaluate the emotional aspect related to catalepsy induced by intraperitoneal administration of haloperidol. To this end, we analysed ultrasonic vocalizations (UVs) of 22 kHz (indicative of aversion) in rats during the tests of catalepsy, open field and contextual conditioned fear. Systemic administration of haloperidol affected the motor activity, inducing catalepsy and decreasing exploratory activity in the open field. There were no UVs of 22 kHz resulting from treatment with haloperidol in catalepsy or open field tests. In the contextual conditioned fear test, haloperidol increased freezing when administered before the test, but decreased freezing on test day when administered before training. In this same test, haloperidol decreased the UVs on the day it was administered (training or test). The catalepsy induced by systemic administration of haloperidol seems to have also affected the motor aspect of UVs. In this way, it was not possible to clarify the existence of an aversive emotional state associated haloperidol induced catalepsy

Qualidade microbiológica de peixes e frutos do mar em Botucatu, SP

whole world. The world production of fish is around 100 million tons/year and 70% of them is destined exclusively to the human feeding. The fish and derived have a great importance in the human diet, contributing with ¼ of offer of protein of animal origin. Sseafood and fish are health, because they are rich in proteins, vitamins, micronutrients and insatured fats. However, one of the most important themes, in public health, refers to the safe food. Only in the USA, 76 million case of foodborne diseases occurs every year, with 325 thousand hospitalized and 5 thousand deaths. According to the microbiological parameters, the Brazilian Food Sanitation Standard (Decree No. 12, 2001) defined that fish, roes of fish, crustaceans and mollusks in natura cooled or frozen, no consumed raw should present up to 103 MPN of coagulase positive Staphylococcus in the absence of Salmonella in 25g. Based on this, this work verified the microbiological quality of fish and seafood retailed in supermarkets and fish store in Botucatu city. A hundred samples were analyzed, being 65 frozen (65%) and 35 cooled (35%). The samples included various kinds of fish and seafood. Among the frozen samples, 31 were fish and 34 seafood. About the cooled ones, 28 were fish and 7 seafood. The methods used for analysis are in agreement with APHA (2001). The samples were acquired in 4 establishments (3 supermarkets and a fish store). Ina total of 100 samples, all were negative to coagulase positive Staphylococcus and 2 were positive for Salmonella (2%). According to the results, we conclude that the presence of Salmonella is a risk factor for the consumers' health and these foods should not be consumed raw. In spite of the freezing to be a good conservation way, this process doesn't totally eliminate the pathogens of the food

Criopreservação de embriões bovino produzidos in vitro: estado da arte

In the last years, the embryo in vitro production for every domestic species and mainly for bovine has attained a notorius status. This reproductive biotechnical procedure associate with ultrasound-guided ovum pick up (OPU) has been more and more incorporated and spread in our cattle herds, ranking up Brazil already at the top of the list in number of in vitro embryo produced. Some significant advantages provided, such as the possibility of using the premature or pregnant animals oocytes, without necessarily requiring the use of hormonal treatment, to make it possible to generate pregnancy at a shorter period of time, the rationalization in the use of semen and optimization in the use of sexed semen were determinant factors for OPU/IVP to reach this outstanding position. Nevertheless, right now the possibility of IVP embryo cryopreservation, just now is the biggest impediment for maximizing the use of this biotechnology, due to both lack of efficient methods and low laboratory produced embryo cryotolerance. Nowadays, the most used methods of IVP embryo cryopreservation are: slow freezing and vitrification. Traditionally, slow freezing is still the most used methods for in vivo and in vitro produced embryo cryopreservation. However, more recently vitrification - although still not commercially used in large scale - has been presenting satisfactory results in IVP embryo cryopreservation, according to searches