Valor preditivo do potencial de implantação embrionário pela análise do perfil químico de meios de cultivo por espectrometria de massas (ESI-Q-ToF)

Objective: This case-control study analyzed mass spectrometry fingerprinting patterns of culture media samples used for embryo culture to predict embryo implantation. Methods: The culture medium harvested after embryo transfer of 22 embryos from 13 patients was used for the experiments. After embryo transfer, the remaining culture media were collected and samples were split in positive (n=8) and negative (n=14) implantation groups according to implantation outcomes (100% or 0% of implantation). Samples were individually diluted and injected directly to the Electrospray ionization (ESI) MS coupled to a Quadrupole Time-of-flight MS (Q-ToF-MS).Ions relative intensities of each spectrum were considered. Data analysis was conducted in MatLab 7.0 version using Partial Least Squares - Discriminant Analysis toolbox. Results: There were 3027 observed ions at 100% and 0% implantation groups by ESI-Q-ToF-MS. The statistical model could categorize the samples in two clusters, based on their positive and negative implantation outcomes. Less intense ions present in the mass spectra with statistical significance have contributed to the major differences to group distinction. Conclusions: Positive and negative implantation embryos showed a specific biochemical pattern present in culture media, which could be detected as a fast, simple and non-invasive way. This biochemical profile could help the selection of the most viable embryo, improving single embryo transfer and thus eliminating the risk and undesirable outcomes of multiple pregnancies. © Todos os direitos reservados a SBRA - Sociedade Brasileira de Reprodução Assistida.

Utilização de meios alternativos e diferentes tipos de recipientes no cultivo de Ankistrodesmus gracilis (Reinsch) Korshikov (Chlorophyceae) em laboratório

A laboratory culture of Ankistrodesmus gracilis algae was evaluated by studying the biology of the species and its chemical composition in a traditional medium (CHU 12) and in two alternative culture media, NPK (20-5-20) and macrophyte (Eichhornia crassipes) + NPK, in three different types of recipients (fiberglass, carboy and plastic bag). First peak in the growth curve of Ankistrodesmus gracilis occurred on the ninth day in macrophyte + NPK medium (74.16 x 10 5 cells mL -1) in a fiberglass recipient. However, highest density (p < 0.01) was reported in medium CHU 12 (122.87 x 10 5 cells mL -1) in a plastic bag on the twelfth day. Cell density was over 70 x 10 5 cells mL -1 starting on the twelfth day. Growth rate of A. gracilis was similar (p > 0.05) in culture media in the three recipients. Protein and fiber were similar (p > 0.05) in the treatments, but lipids were higher (p < 0.05) in NPK. Nitrate, ammonia, total phosphorus and orthophosphate contents were over 1 mg L -1 in NPK (p < 0.01). Results show that alternative media, such as NPK and macrophyte + NPK, are possible for large-scale culture of A. gracilis cultured in three types of recipients. Costs are low, occupying less space when cultured in plastic bags and in the laboratory.

Crescimento inicial do eucalipto em função da aclimatação em viveiro

This study aimed to evaluate the acclimatization effects in the Eucalyptus grandis vs. Eucalyptus urophylla seedlings nursery in their initial growth in two soils types, clay and sandy. The seedlings were planted in Plantmax substrate and in rice hulls plus vermiculite, and managed, after 60 days of the mass propagation (DAE), during the rustication. There were five different frequencies of subsurface drip irrigation, restoring the soil field capacity condition: F1, F2, F3 and F4, which were irrigated once, twice, three and four times a day, respectively, and FD, kept in continue irrigation until planting at 90 DAE. In a randomized block design with four replications, plant height (HPA) were evaluated at 6 and 13 months after planting and the diameter at breast height (DAP) at 13 months after planting. Findings show that water management at hardening phase seedlings had no influence on growth in both soils.

Valores bioquímicos séricos de tilapia del Nilo (Oreochromis niloticus) en cultivo intensivo

The aim of the study was to evaluate the serum biochemical parameters of the Nile tilapia (Oreochromis niloticus), Thai Chitralada lineage, raised under an intensive pisciculture system and captured by the cast net technique. Blood samples from 40 individuals were obtained by caudal venopuncture. Mean body weight was 453 ± 52 g. The serum biochemical parameters evaluated were total protein, albumin, globulins, acid uric, creatinine, urea, calcium, inorganic phosphorus, Ca/P ratio, alkaline phosphatase, and magnesium. In general, the data showed similar parameters in comparison to those established for scale fish, exception of the acid uric.

Consumo hídrico da rúcula em cultivo hidropônico NFT utilizando Rejeitos de dessalinizador em Ibimirim-PE

O consumo de águas salinas pelas plantas na maioria dos casos afetam o rendimento das culturas em suas diversas fases prejudicando desenvolvimento e produção. O trabalho avaliou o consumo hídrico da rúcula (Eruca sativa) em sistema hidropônico NFT com águas salinas. Foram analisados seis níveis crescentes de salinidade da água (CEa), quais sejam: 0,2; 1,2; 2,2; 3,2; 4,2 e 5,2 (dS m-1) utilizaram-se duas fontes de sais: águas salobras (AS) e NaCl. Foi determinado o volume evapotranspirado por planta (VETc) no sistema durante o cultivo. O consumo hídrico nos primeiros dias após transplantio foi de 250 mL dia-1 aproximadamente para ambas as testemunhas e decresceram com o aumento da salinidade, a eficiência de utilização da água foi reduzida revelando uma resposta linear decrescente em função da salinidade ocorrendo uma resposta negativa das plantas que apresentaram uma redução na quantidade de folhas de 3,34%.

Influência de diferentes sistemas de cultivo nos atributos físicos e no carbono orgânico do solo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Diferenças regionais do cultivo de café no Brasil

Brazil is one of the major coffee producers in the world, because of this, the goal of this study was to assess the regional differences of coffee cultivation for the reference crops 2001/2002 and 2002/03 by means of a life cycle assessment (LCA) in order to generate detailed production inventory data as well as quantify the potential environmental impacts of this crop. All information considered in this study (use of water, fossil based energy, fertilizers, pesticides and correctives) was taken from data collected from the producing farms. Four Brazilian coffee producer regions located at the Southeastern region were evaluated: Sul de Minas Gerais and Cerrado Mineiro in Minas Gerais State, and Mogiana and Alta Paulista regions in São Paulo State. The data refer to a production of 25.2 million kg of green coffee. Depending on the considered region, the production of 1,000 kg of green coffee requires, on average, approx. 9,300 to 13,000 kg of total energy, 70 to 130 kg of diesel, 6,500 to 12,700 kg of process water, 270 to 340 kg of fertilizers (NPK), 2.0 to 13.0 kg of pesticides, 230 to 600 kg of correctives, and yield around 1,600 to 1,900 kg/ha. Despite 20% of the coffee growers showing a good environmental performance, i.e. consumption of pesticides, fertilizers and correctives lower than the regional averages, this study has also identified some farms that can probably reduce the amount of some inputs and enhance their environmental performance.

Cultivo del algodón y la soya en el Paraguay y sus derivaciones sociales

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Expansión del cultivo de la caña de azúcar y de la ganadería en el Nordeste del Brasil: políticas públicas y sus derivaciones económicas y sociales

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Efeito competicional em testes de progênies de eucalipto

Many studies have recommended the use of small plots for forest experiments, although they do not consider the inter-genotype competition increase. If this competition is not isolated from the mathematics model, it can lead to incorrect selection of genetic materials. The aim of this work was to evaluate the effect of seven competition covariates in two Eucalyptus spp. progeny tests. Data from the two half-sib eucalyptus progenies were analyzed, using the randomized blocks design. The seven analyzed covariates were HegyI's competition index (IC), self-competition (AT), alo competition (AL), self-competition mean (MAT), alo competition mean (MAL), and arithmetic means of four (M4) and eight (M8) nearest neighbors. Individual and combined analyses of covariates were used for the wood volume trait. All the variance components and the changes caused by covariates use were evaluated. The competition affects the results of eucalypt progeny analysis in different ways, according to its type, self or alo competition. Most influential covariates were MAT, MAL and IC. Most promising results of competition effects reduction were observed for the IC/MAT covariates inclusion in eucalypt progeny tests.

Relação do meio físico com o manejo sustentável de plantio de eucalipto em áreas de terrenos acidentados - A Fazenda Santa Edwirges, Vale do Paraíba, Sudeste do Brasil

This study intends to evaluate the effects of landscape physical elements (rocks and relief) associated with silviculture activities. The study was carried out on a small farm (Fazenda Santa Edwirges) covered by Eucalyptus forested situated in the Paraiba do Sul Basin, Southeast Brazil. The methodology consisted of detailed geological and geomorphological studies at 1:10,000 scale, and laboratory analysis of soil physical properties. The results showed three geologic-geomorphologic associations (ridge escarpment with granitic rocks, steep and gentle hills cut by shear zones and gentle hill with alluvial sediments) present high vulnerability for the development of the physical processes such as accelerated erosion landslides and flooding. In contrast, mountains associated with gneissic rocks present smaller vulnerability and high resilience for the development of the physical processes. The results have showed the importance of considering the interactions among landscape physical for the eucalyptus forest management contributing to a better selection area for eucalyptus cultivation and minimize adverse environment impact in road design.

Necessidade Hídrica E Coeficiente de Cultivo do Pimentão Fertirrigado

This study aimed to determine the crop evapotranspiration (ETc) and crop coefficient (Kc) of fertigated sweet pepper, measured over time (15, 30, 45, 60, 75, 90 and 105 days after the transplant). The experiment was conducted on a battery of 40 drainage lysimeters, arranged in a 8 x 5 matrix, in the period October/2009 to January/2010, located in the Federal Rural University of Pernambuco (UFRPE) Campus in Recife, in a completely randomized factorial scheme 5 x 2, with four replications, being five irrigation depths (80, 90, 100, 110 and 120% of ETc) and two levels of potassium (80 and 120 kg K2O ha-1) where the experimental plot was composed of three plants in each lysimeter. The Mauchly Sphericity Test indicated multivariate variance analysis for the studied variables ETc (mm d-1) and Kc, the variables presented adjustment under quadratic effect of variation on observation times, when the irrigation depths were fixed, and descending linear adjustments under the variation of irrigation depths effect, when the times of observation were fixed in each potassium dose.

Condutividade elétrica da solução nutritiva e acúmulo de macro e micronutrientes no cultivo de crisântemo

The study of growth and uptake of nutrients is important to determine the times of increased demand in order to schedule the fertigation. The present research was developed with the objective of evaluating the effects of different levels of electrical conductivity on growth and accumulation of nutrients in chrysanthemum plants under greenhouse conditions. The electrical conductivity levels on the applied solution were 1.42, 1.65, 1.89, 2.13 and 2.36 dS m-1 (bud vegetative stage); 1.71, 1.97, 2.28, 2.57 and 2.85 dS m-1 (bud stage). The dry mass of the aerial portion of the plant and the contents of macronutrients and micronutrients were determined every 14 days. The nutrient accumulation in chrysanthemum plant showed similar trend as compared to dry matter accumulation. The nutrients presented the following order of absorption: K>N>Ca>Mg>P>S (1425, 892, 184, 150, 110 and 59 mg plant-1) and Fe>Zn>B>Mn>Cu (2254, 2219, 1725, 1287, 210 μg plant-1). The content of most nutrients increased with increase in electrical conductivity of the solution, without salt effect up to EC of 2.85 dS m-1.

Absorção de nitrogênio pelo algodoeiro herbáceo em dois sistemas de cultivo

The study of nutrient uptake is of fundamental importance to plant nutrition, as well as tell the time (growth stage) being absorbed by the culture, also signals to the levels of fertilizer to be used. Aiming to establish the uptake of nitrogen for cultivation of cotton (Gossypium hirsutum L. r. latifolium Hutch.) led to an experiment in a Alfissol under randomized block design with two treatments, sowing conventional tillage and after tillage on the straw of previous crop (millet), with five replicates. Samples were taken according to the phenology and plants development. The dry matter accumulation in cotton is linear and increasing until the period of large boll, being more intense with the appearance of flowers and boll, or 50 to 100 days after emergence. The nitrogen content is higher in newly emerged plants, having peaks at the beginning of the appearance of squares and flowers, and from the appearance of small boll, the nitrogen content in the plant decreases linearly. The extraction of nitrogen is bigger from the appearance of flowers (55-60 days after emergence).

Qualidade de mudas seminais de eucalipto em função dos substratos e fertilização de liberação controlada

For the production of quality seedlings, depending on the physical characteristics of each substrate, more rational nutritional managements, from the of economic-environmental point of view, should be sought. This study aimed to evaluate the development and quality of Eucalyptus grandis × E. urophylla seedlings in terms of substrates and doses of controlledrelease Osmocote® fertilizer. The experiment was conducted with a completely randomized, factorial design, using as substrates: vermiculite+carbonized rice chaff+coconut fiber (1:1:1); vermiculite+coconut fiber (1:1) and vermiculite+coconut fiber (2:1) and doses of 2, 4, 6 and 8kg·m -3 of substrate. The substrates with higher water holding capacity allow seedlings to reach their maximum development in most parameters using lower doses of controlled-release fertilizer. We recommend the application of the controlled-release fertilizer at a dose of 6.0kg·m-3 in vermiculite+carbonized rice chaff+coconut fiber (1:1:1) and vermiculite+coconut fiber (2:1) substrates, and in substrate vermiculite+coconut fiber (1:1) at a dose of 7.0kg·m-3. In each substrate there is a dose range that promotes a greater formation of suitable root systems for planting in the field, which consequently results in seedlings with greater morphological development at nursery.