Soil abiotic factors influence interactions between belowground herbivores and plant roots

Root herbivores are important ecosystem drivers and agricultural pests, and, possibly as a consequence, plants protect their roots using a variety of defensive strategies. One aspect that distinguishes belowground from aboveground plant–insect interactions is that roots are constantly exposed to a set of soil-specific abiotic factors. These factors can profoundly influence root resistance, and, consequently, the outcome of the interaction with belowground feeders. In this review, we synthesize the current literature on the impact of soil moisture, nutrients, and texture on root–herbivore interactions. We show that soil abiotic factors influence the interaction by modulating herbivore abundance and behaviour, root growth and resistance, beneficial microorganisms, as well as natural enemies of the herbivores. We suggest that abiotic heterogeneity may explain the high variability that is often encountered in root–herbivore systems. We also propose that under abiotic stress, the relative fitness value of the roots and the potential negative impact of herbivory increases, which may lead to a higher defensive investment and an increased recruitment of beneficial microorganisms by the plant. At the same time, both root-feeding herbivores and natural enemies are likely to decrease in abundance under extreme environmental conditions, leading to a context- and species-specific impact on plant fitness. Only by using tightly controlled experiments that include soil abiotic heterogeneity will it be possible to understand the impact of root feeders on an ecosystem scale and to develop predictive models for pest occurrence and impact.

Influence of mycobacterial trehalose 6,6'-dimycolate (TDM) on macrophage responses

Mycobacterium tuberculosis, the causative agent of tuberculosis, survives within macrophages by altering host cell activation and by manipulating phagosomal trafficking and acidification. Part of the success of M. tuberculosis as a major human pathogen has been attributed to its cell wall, a unique structure largely comprised of mycolic acids. Trehalose 6,6′-dimycolate (TDM) is the major glycolipid component on the surface of the mycobacterial cell wall. This study examines the contribution of TDM during mycobacterial infection of murine macrophages. Virulent M. tuberculosis was chemically depleted of surface-exposed TDM using petroleum ether extraction. Compared to their native counterparts, delipidated M. tuberculosis showed similar growth in broth culture. Bone marrow-derived macrophages (BMM) or the murine macrophage-like cell line J774A.1 were infected with delipidated M. tuberculosis, and responses were compared to cells infected with native M. tuberculosis. Delipidated M. tuberculosis demonstrated significantly decreased viability in macrophages by seven days after infection. Reconstitution of delipidated organisms with pure TDM restored viability. Infection with native M. tuberculosis led to high cellular production of cytokines (IL-1β, IL-6, IL-12, and TNF-α) and chemokines (MCP-1 and MIP-1α); infection with delipidated M. tuberculosis significantly abrogated responses. Cytokine and chemokine production were restored when delipidated organisms were reconstituted with TDM. Responses were specifically induced by TDM; all measured cytokines were elicited from macrophages incubated with TDM-coated beads, while control beads coated with bovine serum albumin (BSA) did not induce cytokine production. Visualization of mycobacterial localization in J774A.1 cells using fluorescence microscopy revealed that delipidated M. tuberculosis were significantly more likely to traffic to acidic vesicles (lysosomes) than native organisms. Reconstitution with TDM restored trafficking to non-acidic vesicles. Similarly, TDM-coated beads demonstrated significantly delayed localization to acidic vesicles compared to BSA-coated beads. In summary, the interaction of TDM with macrophages may regulate the outcome of M. tuberculosis infection by influencing cellular cytokine production and intracellular localization of organisms. This research has elucidated a novel and necessary role for TDM in survival of virulent M. tuberculosis in host macrophages during in vitro infection. ^

Parental influence on adolescent smoking initiation among Mexican origin youth

Smoking is often initiated in adolescence through trying or experimenting with cigarettes. Smoking initiation is the beginning critical stage in the smoking trajectory often resulting in addiction. This dissertation examined the effect of parenting variables on smoking initiation behavior among 11–14 year old Mexican origin adolescents, a largely understudied group. The participants in this study were part of a population-based cohort of Mexican origin adolescents residing in Houston, Texas. ^ Aim 1 of this study assessed the appropriateness of the Family Life Questionnaire (FLQ) among Mexican origin adolescents. Second order confirmatory factor analysis (CFA) was performed to examine the factor structure of the FLQ and measurement invariance testing was conducted to evaluate the cross-cultural validity of this scale. Aim 2 analyzed cross-sectional associations between parenting variables and adolescent ever tried smoking behavior while aim 3 focused on prospective examination of changes in parenting variables from baseline to final follow-up on ever tried smoking behavior among never smokers. ^ Overall, the results of the CFA indicated that the original factor structure of the FLQ, with alterations, was a good fit for the Mexican origin adolescents. The measurement invariance analysis of the modified FLQ scale indicated adequate measurement invariance. The aim 2 cross-sectional analyses indicated that family cohesion was significantly associated with lower odds of ever tried smoking. Authoritarian parenting was significantly associated with smoking initiation only at the baseline while family conflict was significantly associated with smoking initiation only at the two-year final home visit. The findings from the aim 3 prospective analysis indicated that changes in levels of family cohesion and conflict are important predictors of smoking initiation among those who have never tried smoking. Specifically, perceiving low levels of family cohesion and a decrease in the family cohesion over two years, as well as perceiving high levels of family conflict and an increase in conflict over two years was associated with smoking initiation among never smokers. ^ In general, the findings of this study provide important insights on the links between parenting and adolescent smoking and assist in designing prevention and intervention programs that emphasize the role of family bonding to prevent adolescent smoking behavior. Family education programs for Mexican culture could also highlight the positive effects of authoritarian practices and good family communication to prevent family conflict and subsequent smoking behavior.^

The influence of antibacterial susceptibility and pharmacodynamics on the therapeutic response of infection

This prospective cohort study estimated how antibacterial resistance affected the time until clinical response. Relative rates of improvement and cure were estimated by proportional-hazards regression for 391 patients with culture-confirmed bacterial keratitis who had the ciprofloxacin minimal inhibitory concentration (MIC) measured of the principal corneal isolate and who were treated with ciprofloxacin 0.3% solution or ointment. After adjusting for age and hypopyon status and stratifying by ulcer size, clinic, and ciprofloxacin formulation, the summary rate of clinical improvement with ciprofloxacin therapy was reduced by 42% (95% confidence limits [CL], 3%, 65%) among patients whose corneal isolate's ciprofloxacin MIC exceeded 1.0 μg/mL compared to those with more sensitive isolates. The summary rate of resolution to improvement and cure was reduced by 36% (95% CL, 11%, 53%) among corneal infections having a higher ciprofloxacin MIC. Rate ratios were modified by the size of the presenting corneal ulceration; for ulcer diameters of 4 mm or less and of more than 4 mm, improvement rate ratios were 0.56 (95% CL, 0.31, 1.02) and 0.65 (95% CL, 0.23, 1.80), respectively; resolution rate ratios were 0.63 (95% CL, 0.44, 0.91) and 0.67 (95% CL, 0.32, 1.39), respectively. Sensitivity analysis showed that the summary improvement rate ratio could be maximally overestimated by 24% (95% CL, −29%, 114%) because of informative censoring or by 33% (95% CL, −21%, 126%) from loss to follow up. Based on reported corneal pharmacokinetics of topical ciprofloxacin, the probability of clinical improvement was 90% or more if the ratio of the achievable corneal ciprofloxacin concentration to the corneal isolate's ciprofloxacin MIC was above 8 or the ratio of the area under the 24-hour corneal concentration curve to the ciprofloxacin MIC was greater than 151. This study suggests that corneal infections by bacteria having a higher ciprofloxacin MIC respond more slowly to ciprofloxacin treatment than those with a lower MIC. While the rate of clinical resolution is affected by patient age and clinical severity, antimicrobial susceptibility testing of corneal cultures can indicate the relative effectiveness of antibacterial therapy. A pharmacodynamic approach to treating bacterial keratitis offers the prospect of optimal antimicrobial selection and modification. ^

Predicting bed form roughness: the influence of lee side angle

Flow transverse bedforms (ripples and dunes) are ubiquitous in rivers and coastal seas. Local hydrodynamics and transport conditions depend on the size and geometry of these bedforms, as they constitute roughness elements at the bed. Bedform influence on flow energy must be considered for the understanding of flow dynamics, and in the development and application of numerical models. Common estimations or predictors of form roughness (friction factors) are based mostly on data of steep bedforms (with angle-of-repose lee slopes), and described by highly simplified bedform dimensions (heights and lengths). However, natural bedforms often are not steep, and differ in form and hydraulic effect relative to idealised bedforms. Based on systematic numerical model experiments, this study shows how the hydraulic effect of bedforms depends on the flow structure behind bedforms, which is determined by the bedform lee side angle, aspect ratio and relative height. Simulations reveal that flow separation behind bedform crests and, thus, a hydraulic effect is induced at lee side angles steeper than 11 to 18° depending on relative height, and that a fully developed flow separation zone exists only over bedforms with a lee side angle steeper than 24°. Furthermore, the hydraulic effect of bedforms with varying lee side angle is evaluated and a reduction function to common friction factors is proposed. A function is also developed for the Nikuradse roughness (k s), and a new equation is proposed which directly relates k s to bedform relative height, aspect ratio and lee side angle.

Influence of road noise disturbances on black vulture nesting area: Case of "Cabeza de Hierro" state in Spanish Mediterranean Central Mountain

The real and potential road influence on soundscape is considered a relevant management aspect that can assess the negative effects of massive visitants on such sensitive species -that have been living for centuries in the area-. As a first approach to the study of human disturbances sound impact, acoustic engineering tools allow us to model noise pollution caused by the main road that crosses the state ?Cabeza de Hierro? (M-604). For these preliminary results we use the French method XPS 31-133, recommend at EU level. Noise emission levels in black vulture nesting area are analyzed to understand the influence of human activities on rural areas and road management on biodiversity conservation. This approach develops a useful tool to make compatible the public enjoyment of forest services such as recreation or landscape scenary, the conservation of biodiversity as well as a suitable social and economic activity level ?timber and firewood harvesting, industry?- at the region.

Optimization of a label-free biosensor vertically characterized based on a periodic lattice of high aspect ratio SU-8 nano-pillars with a simplified 2D theoretical model

We have recently demonstrated a biosensor based on a lattice of SU8 pillars on a 1 μm SiO2/Si wafer by measuring vertically reflectivity as a function of wavelength. The biodetection has been proven with the combination of Bovine Serum Albumin (BSA) protein and its antibody (antiBSA). A BSA layer is attached to the pillars; the biorecognition of antiBSA involves a shift in the reflectivity curve, related with the concentration of antiBSA. A detection limit in the order of 2 ng/ml is achieved for a rhombic lattice of pillars with a lattice parameter (a) of 800 nm, a height (h) of 420 nm and a diameter(d) of 200 nm. These results correlate with calculations using 3D-finite difference time domain method. A 2D simplified model is proposed, consisting of a multilayer model where the pillars are turned into a 420 nm layer with an effective refractive index obtained by using Beam Propagation Method (BPM) algorithm. Results provided by this model are in good correlation with experimental data, reaching a reduction in time from one day to 15 minutes, giving a fast but accurate tool to optimize the design and maximizing sensitivity, and allows analyzing the influence of different variables (diameter, height and lattice parameter). Sensitivity is obtained for a variety of configurations, reaching a limit of detection under 1 ng/ml. Optimum design is not only chosen because of its sensitivity but also its feasibility, both from fabrication (limited by aspect ratio and proximity of the pillars) and fluidic point of view. (© 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

Influence of the semantic content of urban sounds in the identity of outer spaces

This paper shows the influence of the semantic content of urban sounds in the subjective evaluation of outer spaces. The study is based on the analysis conducted in three neighboring and integrated urban spaces with a different form of social ownership in the city of Cordoba, Argentina. It shows that the type of sound source present at each site influence, by its semantic content, in the user´s identification and permanence in the place. The noise present in a soundscape is able to have a high semantic content, and therefore the sound has a particular meaning for the perceiver. Every particular social group influences the production of their own sounds and how they perceive them. This allows to consider the sound as one of the factors that define the sense of "place" or "no place" of a certain urban space. Evidently the sounds, and their ability to evoke and characterize the environment, cannot be ignored in the construction and recovery of anthropological sites. This urban culture is unique and specific to every society. Thepublic spaces, with their soundscape, are part of the construction of the urban identity of a city. It is shown that for identical general sound levels present in each of the spaces, the level of annoyance or discomfort, in relation to the subjective acoustic quality, is different. This is the result of the influence of semantic content of the sounds present in each urban space. Coinciding with other similar research, the level of discomfort or annoyance decreases as the presence of natural sounds such as water, the wind in the trees or the birds singing increases, even when the objective values of noise level of natural sounds are higher.

The influence of transparency on self-evaluation as part of the university accreditation process in Peru.

In order to show the choice of transparency as the guiding principle of the accreditation process, the article evaluates its influence on the fundamental subprocess of self-evaluation, thereby confirming that transparency is an essential tool for continuous improvement of academic processes and those of educational quality management. It fosters educational innovation and permits the sustainability of the continuous accreditation process over time, resulting in greater probabilities of university self-regulation through systemization of the process, with the objective of continuous improvement of university degree programs. The article analyzes the influence of transparency on each activity of the self-evaluation process according to the Peruvian accreditation model prepared under the total quality approach, as a reference for other accreditation models, proposing concrete transparency actions and evaluating its influence on the stakeholder groups in the self-evaluation process, as well as on the efficiency and effectiveness of the process. It is concluded that transparency has a positive influence on the training of human capital and the formation of the university?s organizational culture, facilitating dissemination, understanding and involvement of the stakeholder groups in the continuous improvement of accreditation activities and increasing their acceptance of change and commitment to the process. It is confirmed that transparency contributes toward increasing the efficiency index of the self-evaluation process by reducing operating costs through adequate, accessible, timely contribution of information by the stakeholders and through the optimization of the time spent gathering relevant information. In addition, it is concluded that transparency contributes toward increasing the effectiveness index of self-evaluation by facilitating the achievement of its objectives through synthetic, useful, reliable interpretation of the education situation and the formulation of feasible improvement plans based on the adequacy, relevance, visibility, pertinence and truthfulness of the information analyzed.

Asplund versus Lewerentz

La tesis Asplund versus Lewerentz analiza, por el método comparado, los invariantes, las convergencias y las divergencias entre las obras de los arquitectos suecos Erik Gunnar Asplund y Sigurd Lewerentz. Autores del Cementerio del Bosque de Estocolmo, que desarrollaron en colaboración durante más de veinte años, supone una de las obras maestras de la arquitectura escandinava, y uno de los ejemplos de referencia para entender la cultura arquitectónica nórdica moderna en relación a su tradición. Del estudio contrastado de los distintos proyectos y versiones del cementerio, ejecutados conjuntamente o por separado, se obtienen los valores compartidos entre ambos arquitectos, así como sus afinidades o diferencias respecto a un mismo tema. Estos aspectos, extrapolados al resto de sus trabajos, permiten entender sus actuaciones según las referencias e influencias mutuas, y la definición de las variables que configuran sus respectivos espacios arquitectónicos. La investigación se ha estructurado en ocho capítulos: La Escuela Klara, que trata la relación de Asplund y Lewerentz en su época de estudiantes, así como la influencia de sus maestros respectivos; El Grand Tour, que analiza sus viajes y las miradas de cada uno de ellos en sus visitas a los mismos lugares; Los Estudios, que profundiza en sus caracteres, reflejados a través de la comparación de los distintos lugares de trabajo; Las Tumbas, donde se concretan a pequeña escala los temas desarrollados ampliamente en el Cementerio del Bosque, constituyendo así un laboratorio de ideas; La Exposición de Estocolmo de 1930, que vuelve a aclarar las posturas divergentes de ambos, en este caso, en relación a un tema lúdico, y que supuso un giro de su arquitectura y, con ello, un importante apoyo a los cambios sociales del momento; Los Concursos, realizados por separado, donde se observan con precisión las afinidades y las diferencias respecto a un mismo planteamiento inicial; Los Accesos del Cementerio del Bosque, que supone un trabajo conjunto con alternancia de decisiones, en el que se perfila con claridad los intereses específicos de ambos; y por último, las Capillas del Cementerio del Bosque, especialmente la Capilla del Bosque y de la Resurrección, que concretan, en los dos lugares más íntimos del cementerio, las ideas y personalidades opuestas de Asplund y Lewerentz en relación al tema común de la muerte. Las conclusiones de este estudio comparado y centrado en varios temas, determinan una confluencia de intereses en la conexión de sus obras con la topografía como medio de contextualización, en las acciones sobre la vegetación como un aspecto fundamental y no sólo como un acompañamiento a la arquitectura, y en la manipulación de la luz como hecho diferencial del carácter de los distintos espacios. Se reúnen así en esta tesis unas constantes del trabajo de Asplund y Lewerentz, que planteadas muchas veces de forma distinta, apuntan directamente a los sentimientos del Hombre en el momento de la muerte y ante una despedida en relación a la Arquitectura. ABSTRACT The thesis Asplund versus Lewerentz analyses, by means of comparative methodology, the constants, the convergences and the divergences between the works of the Swedish architects Erik Gunnar Asplund and Sigurd Lewerentz. They are the authors of Stockholm’s Woodland Cemetery, which they developed in collaboration over a period of more than twenty years, one of the masterpieces of Swedish architecture and one of the key examples in terms of understanding modern Nordic architectural culture in relation to its tradition. From the contrasted study of the various projects and versions of the cemetery, executed jointly or separately, one obtains the values shared by both architects as well as their affinities or differences with respect to one and the same theme. These aspects, extrapolated from the rest of their works, permit an understanding of their actions according to mutual references and influences, and the definition of the variables that shape their respective architectural spaces. The research is divided into eight chapters: The Klara School, dealing with Asplund and Lewerentz’s relationship during their student days, as well as the influence of their respective teachers; The Grand Tour, which analyses their travels and the viewpoints of each of them on their visits to the same places; The Studies, looks closely at their personalities, reflected by a comparison of their different workplaces; The Tombs, where the themes extensively developed in the Woodland Cemetery are set out on a small scale, thus constituting one of their laboratories of ideas; The Stockholm exhibition of 1930, which again clarifies the diverging postures of the two, in this case, in relation to a recreational topic which represented a turnabout in their architecture and, moreover an important support for the social changes of the age; The Competitions, which they carried out separately, where one closely observes affinities and differences with respect to an initial approach; The Accesses to the Woodland Cemetery, which represents a joint work with alternating decision-making, in which the interests of both are clearly outlined; and finally, the Chapels of the Woodland Cemetery, especially the Woodland Chapel and the Chapel of the Resurrection, which, in the two most intimate spots in the cemetery, embody the opposing ideas and personalities of Asplund and Lewerentz in relation to the common theme of death. The conclusions of this comparative study centred on various topics, determine a confluence of interests in the connection of their works with the topography as a means of contextualization, in actions relating to vegetation as a fundamental aspect and not just an accompaniment to architecture, and in the manipulation of light as a differential factor in the character of the different spaces. Thus this thesis brings together some of the constants of the work of Asplund and Lewerentz which, frequently dealt with in a different manner, point directly to Man’s feelings at the point of death and in the face of a farewell in relation to Architecture.

Fishing effort control regulations influence on stability, safety and operability of small fishing vessels: study of a series of stability related accidents occurred in Spain between 2004 and 2007

Entre los años 2004 y 2007 se hundieron por problemas de estabilidad cinco pesqueros españoles de pequeña eslora, de características parecidas, de relativamente poca edad, que habían sido construidos en un intervalo de pocos años. La mayoría de los tripulantes de esos pesqueros fallecieron o desaparecieron en esos accidentes. Este conjunto de accidentes tuvo bastante repercusión social y mediática. Entre ingenieros navales y marinos del sector de la pesca se relacionó estos accidentes con los condicionantes a los diseños de los pesqueros impuestos por la normativa de control de esfuerzo pesquero. Los accidentes fueron investigados y publicados sus correspondientes informes; en ellos no se exploró esta supuesta relación. Esta tesis pretende investigar la relación entre esos accidentes y los cambios de la normativa de esfuerzo pesquero. En la introducción se expone la normativa de control de esfuerzo pesquero analizada, se presentan datos sobre la estructura de la flota pesquera en España y su accidentalidad, y se detallan los criterios de estabilidad manejados durante el trabajo, explicando su relación con la seguridad de los pesqueros. Seguidamente se realiza un análisis estadístico de la siniestralidad en el sector de la pesca para establecer si el conjunto de accidentes estudiados supone una anomalía, o si por el contrario el conjunto de estos accidentes no es relevante desde el punto de vista estadístico. Se analiza la siniestralidad a partir de diversas bases de datos de buques pesqueros en España y se concluye que el conjunto de accidentes estudiados supone una anomalía estadística, ya que la probabilidad de ocurrencia de los cinco sucesos es muy baja considerando la frecuencia estimada de pérdidas de buques por estabilidad en el subsector de la flota pesquera en el que se encuadran los cinco buques perdidos. A continuación el trabajo se centra en la comparación de los buques accidentados con los buques pesqueros dados de baja para construir aquellos, según exige la normativa de control de esfuerzo pesquero; a estos últimos buques nos referiremos como “predecesores” de los buques accidentados. Se comparan las dimensiones principales de cada buque y de su predecesor, resultando que los buques accidentados comparten características de diseño comunes que son sensiblemente diferentes en los buques predecesores, y enlazando dichas características de diseño con los requisitos de la nueva normativa de control del esfuerzo pesquero bajo la que se construyeron estos barcos. Ello permite establecer una relación entre los accidentes y el mencionado cambio normativo. A continuación se compara el margen con que se cumplían los criterios reglamentarios de estabilidad entre los buques accidentados y los predecesores, encontrándose que en cuatro de los cinco casos los predecesores cumplían los criterios de estabilidad con mayor holgura que los buques accidentados. Los resultados obtenidos en este punto permiten establecer una relación entre el cambio de normativa de esfuerzo pesquero y la estabilidad de los buques. Los cinco buques accidentados cumplían con los criterios reglamentarios de estabilidad en vigor, lo que cuestiona la relación entre esos criterios y la seguridad. Por ello se extiende la comparativa entre pesqueros a dos nuevos campos relacionados con la estabilidad y la seguridad delos buques: • Movimientos a bordo (operatividad del buque), y • Criterios de estabilidad en condiciones meteorológicas adversas El estudio de la operatividad muestra que los buques accidentados tenían, en general, una mayor operatividad que sus predecesores, contrariamente a lo que sucedía con el cumplimiento de los criterios reglamentarios de estabilidad. Por último, se comprueba el desempeño de los diez buques en dos criterios específicos de estabilidad en caso de mal tiempo: el criterio IMO de viento y balance intenso, y un criterio de estabilidad de nueva generación, incluyendo la contribución original del autor de considerar agua en cubierta. Las tendencias observadas en estas dos comparativas son opuestas, lo que permite cuestionar la validez del último criterio sin un control exhaustivo de los parámetros de su formulación, poniendo de manifiesto la necesidad de más investigaciones sobre ese criterio antes de su adopción para uso regulatorio. El conjunto de estos resultados permite obtener una serie de conclusiones en la comparativa entre ambos conjuntos de buques pesqueros. Si bien los resultados de este trabajo no muestran que la aprobación de la nueva normativa de esfuerzo pesquero haya significado una merma general de seguridad en sectores enteros de la flota pesquera, sí se concluye que permitió que algunos diseños de buques pesqueros, posiblemente en busca de la mayor eficiencia compatible con dicha normativa, quedaran con una estabilidad precaria, poniendo de manifiesto que la relación entre seguridad y criterios de estabilidad no es unívoca, y la necesidad de que éstos evolucionen y se adapten a los nuevos diseños de buques pesqueros para continuar garantizando su seguridad. También se concluye que la estabilidad es un aspecto transversal del diseño de los buques, por lo que cualquier reforma normativa que afecte al diseño de los pesqueros o su forma de operar debería estar sujeta a evaluación por parte de las autoridades responsables de la seguridad marítima con carácter previo a su aprobación. ABSTRACT Between 2004 and 2007 five small Spanish fishing vessels sank in stability related accidents. These vessels had similar characteristics, had relatively short age, and had been built in a period of a few years. Most crewmembers of these five vessels died or disappeared in those accidents. This set of accidents had significant social and media impact. Among naval architects and seamen of the fishing sector these accidents were related to the design constraints imposed by the fishing control effort regulations. The accidents were investigated and the official reports issued; this alleged relationship was not explored. This thesis aims to investigate the relationship between those accidents and changes in fishing effort control regulations. In the introduction, the fishing effort control regulation is exposed, data of the Spanish fishing fleet structure and its accident rates are presented, and stability criteria dealt with in this work are explained, detailing its relationship with fishing vessel safety. A statistical analysis of the accident rates in the fishing sector in Spain is performed afterwards. The objective is determining whether the set of accidents studied constitute an anomaly or, on the contrary, they are not statistically relevant. Fishing vessels accident rates is analyzed from several fishing vessel databases in Spain. It is concluded that the set of studied accidents is statistically relevant, as the probability of occurrence of the five happenings is extremely low, considering the loss rates in the subsector of the Spanish fishing fleet where the studied vessels are fitted within. From this point the thesis focuses in comparing the vessels lost and the vessels that were decommissioned to build them as required by the fishing effort control regulation; these vessels will be referred to as “predecessors” of the sunk vessels. The main dimensions between each lost vessel and her predecessor are compared, leading to the conclusion that the lost vessels share design characteristics which are sensibly different from the predecessors, and linking these design characteristics with the requirements imposed by the new fishing control effort regulations. This allows establishing a relationship between the accidents and this regulation change. Then the margin in fulfilling the regulatory stability criteria among the vessels is compared, resulting, in four of the five cases, that predecessors meet the stability criteria with greater clearance than the sunk vessels. The results obtained at this point would establish a relationship between the change of fishing effort control regulation and the stability of vessels. The five lost vessels complied with the stability criteria in force, so the relation between these criteria and safety is put in question. Consequently, the comparison among vessels is extended to other fields related to safety and stability: • Motions onboard (operability), and • Specific stability criteria in rough weather The operability study shows that the lost vessels had in general greater operability than their predecessors, just the opposite as when comparing stability criteria. Finally, performance under specific rough weather stability criteria is checked. The criteria studied are the IMO Weather Criterion, and one of the 2nd generation stability criteria under development by IMO considering in this last case the presence of water on deck, which is an original contribution by the author. The observed trends in these two cases are opposite, allowing to put into question the last criterion validity without an exhaustive control of its formulation parameters; indicating that further research might be necessary before using it for regulatory purposes. The analysis of this set of results leads to some conclusions when comparing both groups of fishing vessels. While the results obtained are not conclusive in the sense that the entry into force of a new fishing effort control in 1998 caused a generalized safety reduction in whole sectors of the Spanish fishing fleet, it can be concluded that it opened the door for some vessel designs resulting with precarious stability. This evidences that the relation between safety and stability criteria is not univocal, so stability criteria needs to evolve for adapting to new fishing vessels designs so their safety is still guaranteed. It is also concluded that stability is a transversal aspect to ship design and operability, implying that any legislative reform affecting ship design or operating modes should be subjected to assessing by the authorities responsible for marine safety before being adopted.

Asplund versus Lewerentz

La tesis Asplund versus Lewerentz analiza, por el método comparado, los invariantes, las convergencias y las divergencias entre las obras de los arquitectos suecos Erik Gunnar Asplund y Sigurd Lewerentz. Autores del Cementerio del Bosque de Estocolmo, que desarrollaron en colaboración durante más de veinte años, supone una de las obras maestras de la arquitectura escandinava, y uno de los ejemplos de referencia para entender la cultura arquitectónica nórdica moderna en relación a su tradición. Del estudio contrastado de los distintos proyectos y versiones del cementerio, ejecutados conjuntamente o por separado, se obtienen los valores compartidos entre ambos arquitectos, así como sus afinidades o diferencias respecto a un mismo tema. Estos aspectos, extrapolados al resto de sus trabajos, permiten entender sus actuaciones según las referencias e influencias mutuas, y la definición de las variables que configuran sus respectivos espacios arquitectónicos. La investigación se ha estructurado en ocho capítulos: La Escuela Klara, que trata la relación de Asplund y Lewerentz en su época de estudiantes, así como la influencia de sus maestros respectivos; El Grand Tour, que analiza sus viajes y las miradas de cada uno de ellos en sus visitas a los mismos lugares; Los Estudios, que profundiza en sus caracteres, reflejados a través de la comparación de los distintos lugares de trabajo; Las Tumbas, donde se concretan a pequeña escala los temas desarrollados ampliamente en el Cementerio del Bosque, constituyendo así un laboratorio de ideas; La Exposición de Estocolmo de 1930, que vuelve a aclarar las posturas divergentes de ambos, en este caso, en relación a un tema lúdico, y que supuso un giro de su arquitectura y, con ello, un importante apoyo a los cambios sociales del momento; Los Concursos, realizados por separado, donde se observan con precisión las afinidades y las diferencias respecto a un mismo planteamiento inicial; Los Accesos del Cementerio del Bosque, que supone un trabajo conjunto con alternancia de decisiones, en el que se perfila con claridad los intereses específicos de ambos; y por último, las Capillas del Cementerio del Bosque, especialmente la Capilla del Bosque y de la Resurrección, que concretan, en los dos lugares más íntimos del cementerio, las ideas y personalidades opuestas de Asplund y Lewerentz en relación al tema común de la muerte. Las conclusiones de este estudio comparado y centrado en varios temas, determinan una confluencia de intereses en la conexión de sus obras con la topografía como medio de contextualización, en las acciones sobre la vegetación como un aspecto fundamental y no sólo como un acompañamiento a la arquitectura, y en la manipulación de la luz como hecho diferencial del carácter de los distintos espacios. Se reúnen así en esta tesis unas constantes del trabajo de Asplund y Lewerentz, que planteadas muchas veces de forma distinta, apuntan directamente a los sentimientos del Hombre en el momento de la muerte y ante una despedida en relación a la Arquitectura. ABSTRACT The thesis Asplund versus Lewerentz analyses, by means of comparative methodology, the constants, the convergences and the divergences between the works of the Swedish architects Erik Gunnar Asplund and Sigurd Lewerentz. They are the authors of Stockholm’s Woodland Cemetery, which they developed in collaboration over a period of more than twenty years, one of the masterpieces of Swedish architecture and one of the key examples in terms of understanding modern Nordic architectural culture in relation to its tradition. From the contrasted study of the various projects and versions of the cemetery, executed jointly or separately, one obtains the values shared by both architects as well as their affinities or differences with respect to one and the same theme. These aspects, extrapolated from the rest of their works, permit an understanding of their actions according to mutual references and influences, and the definition of the variables that shape their respective architectural spaces. The research is divided into eight chapters: The Klara School, dealing with Asplund and Lewerentz’s relationship during their student days, as well as the influence of their respective teachers; The Grand Tour, which analyses their travels and the viewpoints of each of them on their visits to the same places; The Studies, looks closely at their personalities, reflected by a comparison of their different workplaces; The Tombs, where the themes extensively developed in the Woodland Cemetery are set out on a small scale, thus constituting one of their laboratories of ideas; The Stockholm exhibition of 1930, which again clarifies the diverging postures of the two, in this case, in relation to a recreational topic which represented a turnabout in their architecture and, moreover an important support for the social changes of the age; The Competitions, which they carried out separately, where one closely observes affinities and differences with respect to an initial approach; The Accesses to the Woodland Cemetery, which represents a joint work with alternating decision-making, in which the interests of both are clearly outlined; and finally, the Chapels of the Woodland Cemetery, especially the Woodland Chapel and the Chapel of the Resurrection, which, in the two most intimate spots in the cemetery, embody the opposing ideas and personalities of Asplund and Lewerentz in relation to the common theme of death. The conclusions of this comparative study centred on various topics, determine a confluence of interests in the connection of their works with the topography as a means of contextualization, in actions relating to vegetation as a fundamental aspect and not just an accompaniment to architecture, and in the manipulation of light as a differential factor in the character of the different spaces. Thus this thesis brings together some of the constants of the work of Asplund and Lewerentz which, frequently dealt with in a different manner, point directly to Man’s feelings at the point of death and in the face of a farewell in relation to Architecture.

Influence of sequential fermentations with Torulaspora delbrueckii and Saccharomyces cerevisiae on wine quality

Torulaspora delbrueckii is a non-Saccharomyces yeast with interesting metabolic and physiological properties of potential use in oenology. This work examines the fermentative behaviour of five strains of T. delbrueckii in sequential fermentations with Saccharomyces cerevisiae, analysing the formation of aromatic compounds, polyalcohols and pigments. The fermentative power of these five strains ranged between 7.6 and 9.0% v/v ethanol; the associated volatile acidity was 0.2e0.7 g/l acetic acid. The production of glycerol was inferior to that of S. cerevisiae alone. The mean 2,3-butanediol concentration reached in single-culture S. cerevisiae fermentations was 73% higher than in the five sequential T. delbrueckii/S. cerevisiae fermentations. However, these fermentations produced larger quantities of diacetyl, ethyl lactate and 2-phenylethyl acetate than single-culture S. cerevisiae fermentation. 3-ethoxy propanol was produced only in the sequential fermentations. The five sequential fermentations produced smaller quantities of vitisin A and B than single-culture S. cerevisiae fermentation. In tests performed prior to the addition of the S. cerevisiae in the sequential fermentations, none of the T. delbrueckii strains showed any extracellular hydroxycinnamate decarboxylase activity. They therefore produced no vinyl phenolic pyranoanthocyanins.