Efeito da oxigenação hiperbárica no reparo ósseo de ratos diabéticos

The chronic state of hyperglycemia due to diabetes mellitus affects multiples organs impairing life quality. In bone, diabetes alters strength and mineral density and also suppresses the osteoblast activity, leading to an unbalanced bone healing process. Hyperbaric oxygen therapy (HBO) is suggested as an adjuvant treatment to accelerate bone repair. This study evaluated the effects of HBO in the number of mast cells and in new bone formation at the initial stage of bone repair in normoglycemic and diabetic rats. It was hypothesized that HBO treatment may improve bone repair in diabetic bone. The rats were equally divided in four groups: Control (C); Control + HBO (CH); Diabetes (D) and Diabetes + HBO (DH). Diabetes was induced by streptozotocin (65mg/kg) and femoral bone defects were created thirty days after diabetes induction in all groups. HBO initiated immediately after surgery procedure and was performed daily, for 7 days, in the CH e DH groups. Seven days after surgery, all animals were euthanized. The femur diaphyses were removed, fixated, decalcified and processed for paraffin embedding. The semi-serial histological sections obtained were stained with Hematoxylin-Eosin (HE), Mallory Trichrome and Toluidine Blue. The qualitative analysis was conducted in the histology slides stained with HE, where it was evaluated the morphological aspects of bone repair in the lesion area, observing the presence of clot, inflammatory cells, granulation tissue, type of bone tissue, morphology of bone cells, and thickness and organization of bone trabeculae. In the slides stained with Mallory Trichrome and Toluidine Blue were evaluated the percentage of new bone formation and number of mast cells, respectively. The qualitative analysis showed that the CH group presented a more advanced stage of bone repair compared to the C group, showing thicker trabeculae and greater bone filling of the lesion area. In D and DH group, the lesion area was partially filled with new bone formation tissue and presented thinner trabeculae and fewer areas associated to osteoclasts compared to control group. The histomorphometric analysis showed a significant improvement in new bone formation (p<0.001) comparing CH (38.08 ± 4.05) and C (32.05 ± 5.51); C and D (24.62 ± 2.28 and CH and DH (27.14 ± 4.21) groups. In the normoglycemic rats there was a significant increasing in the number of mast cells (p<0.05) comparing C (8.06 ± 5.15) and CH (21.06 ± 4.91) groups. In conclusion, this study showed that diabetes impaired bone repair and HBO was only able to increase new bone formation and the number of mast cells in the normoglycemic animals.

Atividade antiangiogênica da forma recombinante da proteína 21 de Trypanosoma cruzi

Chagas disease, caused by the parasite Trypanosoma cruzi, is the cause of Chronic chagasic cardiomyopathy (CCC). The prospection of innovative therapeutic agents against CCC is a major task. The recombinant form of 21 (rP21), a secreted T. cruzi protein involved in host cell invasion and on progression of chronic inflammatory processes have been studied as a potential novel therapeutic target. Our present work aimed to verify and investigate the impact of rP21 in the formation of blood vessels in vitro and in vivo. First, tEnd cells were treated with different concentrations of rP21 or bacterial extract and viability and cellular adhesion were evaluated by MTT and angiogenesis inhibition by Matrigel tube formation assay and murine model. To verify the proteolytic activity of rP21 on extracellular matrix (ECM) components, fibrinogen, matrigel and fibronectin was incubated with rP21 or not. In addition, we performed proliferation assays and cell cycle analysis. Furthermore, the accumulation and distribution of F-actin was determined by Phalloidin staining using ImageJ software. Finally, tEnd cells were incubated with rP21 and the mRNA levels were analyzed by real-time PCR. Our results showed that rP21 did not alter cell viability and adhesion, but strongly inhibited vessel formation in vitro and in vivo. Tube formation assay showed that angiogenesis inhibition was dependent of the CXCR4-rP21 binding. In addition to these results, we observed that the rP21 was able to inhibit cell proliferation and promoted a significant reduction in the number of 4n cells (G2/M phase). Moreover, we found that rP21 significantly increased F-actin levels and this protein was able to modulate expression of genes related to angiogenesis and actin cytoskeleton. However, rP21 showed no significant activity on the matrix components. In this sense, we conclude that the rP21-endothelial cells (ECs) interaction via CXCR4 promotes inhibition of vessel formation through a cascade of intracellular events, such as inhibition of ECs proliferation and modulation of the expression of molecules associated with angiogenic processes and actin cytoskeleton.

Rate of exposure of a sentinel species, invasive American mink (Neovison vison) in Scotland, to anticoagulant rodenticides

Open Access funded by Natural Environment Research Council Acknowledgements Field sample collection was funded by NERC grants NE/E006434/1 and NE/J01396X/1 to XL and a Marie Curie FP7-PEOPLE-2011-IEF 300288-grant to YM. We thank the Scottish Mink Initiative, staff, funders and multiple mink volunteers for the continued effort, samples and data. Also to Eduardo Salazar Villaverde for his assistance in the preparation of figures in early drafts of this manuscript, and Professor Colin Prescott (Reading University) for comments on the biochemistry of ARs. Finally, to Kenneth McNeill for providing data on farm sizes and distributions.

The Philippines in the Electronics & Electrical Global Value Chain

This report uses the Duke CGGC global value chain (GVC) framework to examine the role of the Philippines in the global electronics & electrical (E&E) industry and identify opportunities to upgrade. Electronics and electrical equipment have played an important role in the Philippine economy since the 1970s and form the foundation of the country’s export basket today. In 2014, these sectors accounted for 47% of total exports from the Philippines at US$28.8 billion, of which 41% was from electronics, and 6% from electrical products. From a global perspective, while the Philippines is not the leading exporter in any particular product category, it is known for its significant number of semiconductor assembly and test (A&T) facilities. The global economic crisis (2008-09), combined with the exit of Intel (2009), had a significant negative impact on electronics exports and, although steadily increasing, they have not yet rebounded to pre-crisis levels. Nonetheless, investment in the E&E industries has picked up since 2010; in the past five years, there have been 110 new investments in these sectors. Another positive sign is the low exit rate; with the exception of Intel, companies that have invested in the Philippines have stayed, with several operations dating back to the late 1970s and 1980s. These firms have not only stayed, but have continued to grow and expand in the country due to the quality of the workforce and satisfaction with the Philippine Economic Zone Authority (PEZA) environment. The growth of the industry has significantly benefited from foreign investment and close ties with Japanese firms.

Global Value Chain Analysis: A Primer, 2nd Edition

The first edition of Global Value Chain Analysis: A Primer was released five years ago (May 2011) in order to provide an overview of the key concepts and methodological tools used by Duke University’s Center on Globalization, Governance & Competitiveness (Duke CGGC) a university-based research center that focuses on innovative applications of the GVC framework, which was developed by Duke CGGC’s founding director, Gary Gereffi. The Second Edition of Global Value Chain Analysis: A Primer (July 2016) retains a simple, expository style and use of recent research examples in order to offer an entry point for those wishing to better understand and use the GVC framework as a tool to analyze how local actors (firms, communities, workers) are linked to and affected by major transformations in the global economy. The GVC framework focuses on structural shifts in global industries, anchored by the core concepts of governance and upgrading. This Second Edition highlights some of the refinements in these concepts, and introduces a number of new illustrations drawing from recent Duke CGGC research. The bibliography offers a sampling of the broad array of studies available on the Duke CGGC website and in related academic publications. We hope this work stimulates continued interest in and use of the GVC framework as a tool to promote more dynamic, inclusive and sustainable development outcomes for all economies and the local actors within them.

Mechanisms Governing the Eyewall Replacement Cycle in Numerical Simulations of Tropical Cyclones

Eyewall replacement cycle (ERC) is frequently observed during the evolution of intensifying Tropical Cyclones (TCs). Although intensely studied in recent years, the underlying mechanisms of ERC are still poorly understood, and the forecast of ERC remains a great challenge. To advance our understanding of ERC and provide insights in improvement of numerical forecast of ERC, a series of numerical simulations is performed to investigate ERCs in TC-like vortices on a f-plane. The simulated ERCs possess key features similar to those observed in real TCs including the formation of a secondary tangential wind maximum associated with the outer eyewall. The Sawyer-Eliassen equation and tangential momentum budget analyses are performed to diagnose the mechanisms underlying the secondary eyewall formation (SEF) and ERC. Our diagnoses reveal crucial roles of outer rainband heating in governing the formation and development of the secondary tangential wind maximum and demonstrate that the outer rainband convection must reach a critical strength relative to the eyewall before SEF and the subsequent ERC can occur. A positive feedback among low-level convection, acceleration of tangential winds in the boundary layer, and surface evaporation that leads to the development of ERC and a mechanism for the demise of inner eyewall that involves interaction between the transverse circulations induced by eyewall and outer rainband convection are proposed. The tangential momentum budget indicates that the net tendency of tangential wind is a small residual resultant from a large cancellation between tendencies induced by the resolved and sub-grid scale (SGS) processes. The large SGS contribution to the tangential wind budget explains different characteristics of ERC shown in previous numerical studies and poses a great challenge for a timely correct forecast of ERC. The sensitivity experiments show that ERCs are strongly subjected to model physics, vortex radial structure and background wind. The impact of model physics on ERC can be well understood with the interaction among eyewall/outer rainband heating, radilal inflow in the boundary layer, surface layer turbulent processes, and shallow convection in the moat. However, further investigations are needed to fully understand the exhibited sensitivities of ERC to vortex radial structure and background wind.

Geochemistry of sheeted dike complex minerals of ODP Hole 140-504B

Diabases were recovered during Legs 137 and 140 at Hole 504B from depths between 1621.5 and 2000.4 meters below seafloor in the lower sheeted dike complex. The samples contain multiple generations of millimetric to centimetric veins. The orientation of the measured veins suggests that two main vein sets exist: one characterized by shallow dipping and the other by random trend. Thermal contraction during rock cooling is considered the main mechanism responsible for fracture formation. Vein infill is related to the circulation of hydrothermal fluids near the spreading axis. Some veins are surrounded by millimeter-sized alteration halos due to fluid percolation from the fractures through the host rock. Vein-filling minerals are essentially amphibole, chlorite, and zeolites. Amphibole composition is controlled by the microstructural site of the rock. Actinolite is the main amphibole occurring in the veins and also in the groundmass away from the halos. In the alteration halos, amphibole shows composition of actinolitic hornblende and Mg-hornblende. Late-stage tension gashes and interstitial spaces in some amphibole-bearing veins are filled with zeolites, suggesting that the veins likely suffered multiple opening stages that record the cooling history of the circulating fluids. Evidence of deformation recorded by the recovered samples seems to be restricted to veins that clearly represent elements of weakness of the rock. On the basis of vein geometry and microstructure we infer structural interpretations for the formation mechanism and for deformation of veins.

Age derminations of rocks from the Read Mountains, Antarctica

The Shackleton Range can be divided into three major units: (1) The East Antarctic Craton and its sedimentary cover (Read Group and Watts Needle Formation), (2) the allochthonous Mount Wegener Nappe (Mount Wegener Formation, Stephenson Bastion Formation, and Wyeth Heights Formation), and (3) the northern belt (basement: Pioneer and Stratton Groups, sedimentary cover: Haskard Highlands Formation (allochthonous?), and Blaiklock Glacier Group). The northern units are thrust over the southern ones. The thrusting is related to the Ross Orogeny. The Mount Wegener Nappe, which appears to be a homogeneous tectonic unit, consists of a Precambrian basement (Stephenson Bastion Formation, Wyeth Heights Formation?) and a Cambrian cover (Mount Wegener Formation). Some questions are still open for discussion: the position of the Haskard Highlands Formation (trilobite shales) may be erratic or represent a tectonic sliver, the relation of the former Turnpike Bluff Group, the origin of the crystalline basement west of Stephenson Bastion and others.

A Developmental Approach to Sport Expertise

This chapter examines the personal and contextual factors of youth sport that affect sport expertise and developmental outcomes. The developmental model of sport participation (DMSP) is used as a comprehensive framework that outlines different pathways of involvement in sport. Activities and contexts that promote continued sport participation and expert performance are discussed as the building blocks of all effective youth sport programs. This chapter provides evidence that performance in sport, participation, and psychological development should be considered as a whole instead of as separate entities by youth sport programmers. Adults in youth sports (i.e. coaches, parents, sport psychologists, administrators) must consider the differing implications of concepts such as deliberate play, deliberate practice, sampling, specialization, and program structure at different stages of an athlete's talent development. Seven postulates are presented regarding important transitions in youth sport and the role that sampling and deliberate play, as opposed to specialization and deliberate practice, can have during the childhood in promoting continued participation and elite performance in sport

The Neolithic of El Niño cave (Ayna, Albacete) in the Sierra del Segura context

Since its excavation in the summer of 1973, El Niño cave has been considered a key site to understand the process of production economy and pottery technology introduction in South-eastern Iberian Peninsula, and especially to approach how such process could have affected people already settled in the Segura mountains. However, data from El Niño cave was very fragmentary, due to the lack of a broad study of Neolithic occupations of the site. In this paper, we present the analysis of pottery, lithic industry and faunal remains, as well as the existing dates from the site´s Holocene levels. The review of different evidence from the site allows suggesting that El Niño cave would have probably acted as a hunting and shepherding station, being a logistical site of larger places. However, limitations due to the fact that we are dealing with a 40- year-old excavation, prevent specifying how the process of Neolithic introduction in the Segura Mountains occurred.

A Institucionalização no Feminino: que repercussões na reintegração: trajectórias de vida e dimensão dos factores de (des) protecção

A presente dissertação – A Institucionalização no Feminino: que repercussões na Reintegração (Trajectórias de vida e dimensão dos factores de (des) protecção na transmissão intergeracional das jovens que saíram da CIJE de Castelo Branco no período de 1995-2000) – foi realizada no âmbito do VI Curso de Mestrado em Serviço Social. O objecto desta dissertação centrou-se na análise das trajectórias de vida de mulheres adultas que durante a sua infância estiveram internadas na Instituição Casa de Infância e Juventude de Castelo Branco e cuja desinstitucionalização ocorreu no período de 1995 a 2000, considerando como critério a residência das jovens no Distrito de Castelo Branco. Para a prossecução desta investigação, adoptou-se uma metodologia qualitativa. Recorreu-se à análise documental e a entrevistas semi-directivas efectuadas a nove jovens. Com esta investigação pretendeu-se estudar as trajectórias para a obtenção de dados sobre a experiência de vida, o seu processo de autonomização e de que forma a medida de institucionalização se reproduziu nos descendentes, e se estes constam igualmente das crianças e jovens com medida de protecção, nomeadamente de acolhimento institucional. Relativamente aos objectivos, pretendemos: analisar o percurso de vida das jovens com medida de acolhimento institucional; identificar a avaliação das jovens sobre o seu processo de internamento e a experiência de vida; identificar a forma como estas jovens se relacionam com a percepção do conceito formal da lei de “Perigo”; analisar a autonomia de vida face à família, à inserção profissional e eventual ligação face aos sistemas de protecção social; e analisar a situação e as expectativas face às jovens sem filhos. Dos resultados obtidos na presente investigação, podemos concluir que, após a medida de internamento, a maioria das jovens integrou a família de origem. Algumas valorizam este regresso, ressalvando as dificuldades de adaptação à sua família, e à própria comunidade. A maioria das jovens constituíram agregado próprio, revelando capacidades parentais na educação dos seus filhos, e proporcionando-lhes uma infância segura e feliz, assegurando de forma responsável as suas necessidades tendo em vista a sua segurança, saúde, formação, educação e desenvolvimento.

O Combate ao Analfabetismo em Angola desde o Acordo de Paz (2002): alfabetização e os seus constrangimentos no meio rural

Esta investigação debruça-se sobre a análise do Combate ao Analfabetismo em Angola e o processo de alfabetização levado a cabo pelo Estado e os Parceiros Sociais no meio rural, desde o Acordo de paz em 2002, e especificamente, sobre dos Programas, Estratégias e Planos de Alfabetização do Estado, no contexto do Sistema de Educação, preconizados nesse período. Em Angola a maior parte das ações de alfabetização são asseguradas pelos parceiros sociais do Estado, pelo que procedemos à identificação das Organizações, Associações Nacionais e Organizações Internacionais em processos de Alfabetização e aos Métodos de Alfabetização associados ao Plano Estratégico para a Revitalização da Alfabetização (2012). Os constrangimentos ao processo de alfabetização no meio rural foram analisados em três vertentes: do acesso à educação e alfabetização de jovens e adultos; dos professores e alfabetizadores, sua formação, recrutamento e fixação, e o da utilização da Língua Oficial e das Línguas Nacionais. A investigação teve por base uma metodologia do tipo qualitativo assente na recolha e análise bibliográfica e documental, constituindo um estudo interpretativo fruto de uma Revisão Bibliográfica Narrativa. A gratuitidade, a equidade e a qualidade do ensino em Angola, enquanto objetivos primordiais dos principais documentos reitores do Sistema de Educação, não estão a ser asseguradas ou cumpridas com a amplitude e a eficácia pretendidas. Não obstante as estratégias, os programas e os planos de alfabetização concebidos após 2002, a sua implementação não tem conseguido materializar-se enquanto instrumento facilitador de uma “segunda oportunidade” educativa para jovens e adultos analfabetos. Perpetuam-se problemas no acesso ao ensino no meio rural, particularmente no que respeita à gratuidade, aos fracos apoios e incentivos financeiros do Estado e à insuficiente aplicação de políticas de ação social escolar. Persistem também a escassez de professores e alfabetizadores, as suas dificuldades de caracter formativo e profissional, a limitação de recursos de que dispõem e de incentivos que os levem a fixar-se. A utilização das línguas maternas não se efetiva no processo ensino-aprendizagem, onde a sua pertinência é maior devido à identidade bantu das populações. Estes constrangimentos repercutem-se no insucesso escolar dos alunos e no aumento das taxas de abandono/desistência e comprometem o direito à educação. Apesar do esforço nacional, a afetação de recursos do orçamento destinado ao sector da educação, ao combate ao analfabetismo e à alfabetização de jovens e adultos é ainda inferior a metade do recomendado internacionalmente nesta área. / This study focuses on the analysis of the struggle against Illiteracy in Angola and on the literacy process undertaken by the State and the Social Partners in the rural areas, since the Peace Agreement in 2002, and specifically on the Programs, Strategies and Plans of Literacy of the State in the context of the Education System proclaimed on that period. In Angola most literacy actions are ensured by the social partners of the State, therefore we proceed to identify the Organizations, National Associations and International Organizations in processes of Literacy and the Methods of Literacy associated to the Strategic Plan for the Revitalization of Literacy(2012). The constraints on the process of literacy in rural areas were analyzed in three strands: the access to education and literacy for young people and adults; the teachers and literacy teachers, their school formation, recruitment and fixation, and the use of the Official Language and the National Languages. The study used a qualitative methodology based on bibliographic and documentary collection and analysis, constituting an interpretative study, which is the result of a bibliographic narrative review. Free, equal and quality education in Angola as primary objectives of the main guiding documents of the Education System, are not being provided or accomplished with the scope and effectiveness required. Despite the Strategies, the Programs and the Literacy Plans designed after 2002, its implementation has not been able to be materialized as a facilitator of a "second chance" education for young people and adult illiterates. Problems remain in the access to education in rural areas, particularly, as far as free education, weak support and financial incentives from the State and inadequate implementation of the policies of school social work are concerned. The shortage of teachers and literacy teachers, the difficulties related to their training and their profession, the limited resources available and incentives that lead them to settle down also persist. The use of the mother tongues is not effective in the teaching-learning process, where its pertinence is greater due to the bantu identity of the populations. These constraints have repercussions on the students' school failure and on the abandonment rates increase and compromise the right to education. Despite the national effort, the allocation of budget resources intended for the education sector, to fight illiteracy and to improve young people and adults’ literacy, is still less than half of the recommended internationally in this area.

Grounded Theory methods in child psychotherapy research

This article considers the place of qualitative research in psychoanalysis and child psychotherapy. It discusses why research methodology for many years occupied so small a place in these fields, and examines the cultural and social developments since the 1960s which have changed this situation, giving formal methods of research much greater significance. It reflects on the different pressures to develop formal research methods which arise both from outside the psychoanalytic field, as a condition of its continued professional survival, and from within it, where its main aim is the development of fundamental psychoanalytic knowledge, It suggests that the conduct of mainly quantitative research into treatment outcomes is largely a response to these external pressures, whilst the main benefits to be gained from the development of qualitative research methods, such as Grounded Theory, are in facilitating the knowledge-generating capacities and achievements of child psychotherapists themselves. The paper describes Grounded Theory methods, and explains how they can be valuable in the recognition of hitherto unrecognised meanings and patterns as these are made visible in clinical practice. Finally, it briefly describes five different examples of completed doctoral studies, all of which have added significantly to the knowledge-base of child psychotherapy, and which demonstrate how much can be accomplished using this method of research.