954 resultados para Biomarcadores de contaminação


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The Thermal Corridor of Uruguay River is located in the triple border of Argentine, Brazil and Uruguay, and shows an intense economic thermal tourism activity, mainly based on groundwaters from Guarani Aquifer System (GAS). Recent studies have pointed out the occurrence of high concentrations of arsenic (>10 μg/L) in GAS groundwater in this area. The complex geological and hydrogeological framework in the area is associated to the Paraná Basin geological evolution south of the Assuncion-Rio Grande Arch; it encompass paleozoic marine sequences and continental sequences permian/eotriassic to mesozoic in age, which are covered by basaltic lavas of Serra Geral Formation. Iron oxide coatings have been described in sandstones of Buena Vista and Sanga do Cabral formations, which underlie GAS units. Arsenic occurrence is associated to sodium bicarbonate groundwater with pH values over 8.0. Arsenic is released to groundwater by desorption from iron oxides/hydroxides, as result of the higher pH of these waters, indicating that arsenic is released from the units that underlie GAS units. Increase in chromium and uranium concentrations are also related to high pH groundwaters, thus indicating special care on using groundwater from this region.

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The Neoproterozoic granitogenesis related to the Central Mantiqueira Province comprise the calc alkaline to alkaline granitoid complexes of Sorocaba, San Francisco, São Roque, Ibiúna and Piedade. These complexes occur in a ruptil tectonic to tardi (Sn+3) event. The emplacement of the different facies in transtractives structures of the pull-apart type are characterized in the area by the main transcurrent shear zones of Taxaquara-Pirapora, Itu-Jundiuvira, Moreiras, Cangüera and Caucáia of ENE-WSW general direction. The massifs present complex internal architecture characterized by intrusions in restrict initial phase of intermediate equigranular nature. Also present a main phase of porfiroid monzo and sienogranite that fragments the previous phase, followed by lateral accretion of equi to inequigranular material, and in some cases by the accretion of late phases of circular bodies of porfiroid rapakivi granites, and a late to final phase of aplitic to pegmatitic composition. This magmatism grew with the intrusions of successive magmatic pulses, partially controlled by many reactivations of the shear zones. The REE also suggest that the magmatic phases are similar, synchronous and repetitive in four of the complexes in both domains, present in the São Francisco Complex. The crystallization starts from accretion processes, but compositionally quite different from the others. The variation in compositions and ages (TDM) for these granites reflect the derivation from different sources developed under different magmatic conditions, followed by processes of contamination that frequently occur in the crust.

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Water is the raw material used most in the production of diverse pharmaceutical forms and, being a constituent of the formulation itself, is subject to a number of physico-chemical and microbiological specifications. In addition, it is indispensable for laboratory tests and the cleaning of equipment and apparatus. The aim of this study was to ascertain the degree of physicochemical and microbiological contamination of purified water used in compounding pharmacies in the city of São José do Rio Preto, SP, Brazil. Samples were taken as recommended in the USP Pharmacopeia, with careful aseptic technique, and sent immediately the to quality control laboratory. Physicochemical properties were analyzed, including appearance, pH, conductivity, residue after evaporation, ammonia, calcium, chloride, heavy metals, sulfate and oxidizable substances, and microbiological tests were performed: total aerobic microbial count and detection of total and thermotolerant coliforms and Pseudomonas aeruginosa. Results showed that some parameters did not conform to the standards, especially pH, conductivity, inorganic impurities, oxidizable substances and microbiological test data, in 10%, 17%, 10%, 14% and 20% of the analyzed samples, respectively, This points to the need for greater care in the production and/or storage of purified water in these pharmaceutical establishments.

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By remote sensing, geodatabase digital processing, information and expeditions to Capivara's sub-basin, was possible to identify the changes in the landscape mainly the expansion of eucalyptus, sugar-cane, and orange trees, being the last two, mainly on the Periferic Depression of Basaltic Cuesta. A significant portion of the soil on this geological area is formed from sandstones, providing high permeability to them, making them important places to groundwater recharge areas as sensible to contamination by pesticides. Throughout last decade was observed that the native vegetation fragments stabilization, keeping a reason of 26.5% on the land used between 2000 and 2010. The pasture decrease being substituted by eucalyptus, sugar-cane and orange trees call attention for the changes in the agribusiness model demanded by the current economic and social necessity. Pasture decrease followed by erosions decrease on sub-basin is evidence that these two aspects are strongly related.

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Studying the physical environment of a watershed is the basic condition for a successful planning of the riparian forest preservation, and for water production and conservation. The aims of the present study were to analyze and quantify the spatial and temporal evolution (1984 and 2010) using Landsat-5 satellite images of Cintra Stream sub-watershed, Botucatu, São Paulo State, Brazil, processed by the software IDRISI Andes, as well as to analyze the water quality through the parameters pH, EC, DO and BOD5 at 4 different sites in the years 1999, 2008 and 2009. Considering the 1076.48ha area of the sub-watershed, the pasture class of 1984 was reduced by 25.55% in 2010, resulting in an increase in the remaining classes. The most important class was native forest and reforestation since it had an increase of 5.08%, which indicates recovery of the riparian forest. Degraded areas were identified close to the inferior limit of the sub-watershed (P3 and P4), as well as local contamination (P1 and P2) with worsening of the water quality in the remaining sites in the periods 2008 and 2009. Recovery and management of the ecological succession of degraded areas and water quality monitoring at 1 and 2 sites will be necessary to reestablish the natural condition of the area studied.

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Two biomarkers of oxidative stress were evaluated in healthy volunteers treated with daily doses of orange juice that is well known source of vitamin C and citric flavanones, which have been associated with antioxidant, anti-inflammatory and hypolipidemic effects. The antioxidant capacity in the blood serum was evaluated by studying the scavenging of 2,2'-diphenyl-1-picrylhydrazyl (DPPH) free radical, and also lipid peroxidation was evaluated by the thiobarbituric acid reacting substances (TBARS) assay. The results have shown that the regular consumption of orange juice increased 150% and 200% the serum antioxidant capacity for women and men respectively, but it was no significant change in the serum lipid peroxidation. In conclusion, the increase of flavonones and vitamin C in the body due to the regular intake of orange juice expressively improved the antioxidant capacity, but without significant effect on the lipid peroxidation.

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The Ji-Paraná city (RO) it doesn't possess public system of collection and treatment of sewers, being the waters residuárias produced by the local population thrown at sewages. Traditionally, many inhabitants use wells amazon extracted underground water or tubular shallow in the urban zone. The study accomplished in the Nova Brasília neighborhood for Silva (2009) revealed that the local aquifer is strongly contaminated for nitrate, originated of the decomposition of the organic matter deposited at the sewages local maidservants. With the objective of detecting areas with high concentrations originating from organic compositions of septic sewages, geophysical risings were accomplished, later related with analyses physical-chemistries in samples of groundwaters obtained in several wells installed in the Nova Brasília neighborhood, besides of soil samples descriptions in zone not saturated obtained in wells. The results obtained by the geophysical rehearsals they reveal that the polluting feather not migrates through the zone saturated, arriving with relative easiness to the aquifer, reaching in some points, superior depth to 34 m reached by the geoelectrical profiling.

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Bovine mastitis is considered important disease causing major economic losses in dairy herds. Antimicrobial drug can promote resistance, chemical residues in food and environmental contamination. The purpose of this study was to evaluate the antibacterial activity in vitro of pomegranate extract on Staphylococcus aureus isolated from bovine milk, evaluate its antioxidant activity, and quantify levels of total phenols and fravonoides of the different extracts used. Aqueous extracts were used in nature and dry, from the peel of the fruit (EAC) and leaves (EAF). Additionally, it was evaluated the antioxidant activity (AA%), total phenols and flavonoids. Milk samples were inoculated, incubated, and the colonies were characterized as Staphylococcus aureus were adjusted to 1.0x106 UFC/mL in the 6 standard of the MacFarland scale. The sensitivity of the microbial isolates were determined in quintuplicate, in disk diffusion test. The minimum inhibitory concentration was determined by visible inhibition zone greater than 15mm. The results were evaluated by ANOVA, Tukey 5%, using the program SISVAR 5.3 - DEX/UFLA. Results implied that the aqueous extract from the bark of dried fruit was capable of inhibiting bacterial growth at concentrations of 3%. The other treatments only showed this activity, from the concentrations of 15%, 20% and 30% for dry EAF, in nature EAC and in nature EAF, respectively. Regarding the action antioxidant of dry EAC, was not correlated with total phenols and flavonoids. Probably other alkaloids substances present in the extract studied, may have been responsible for this activity. It is concluded that extracts of Punica granatum L., especially those obtained by the shell of the fruit, showed inhibitory activity against S. aureus, indicating your use potential for the control of bovine mastitis.

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Pós-graduação em Ciências Farmacêuticas - FCFAR

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)