Evaluation of the anti-mycobacterium tuberculosis activity and in vivo acute toxicity of annona sylvatic

Background The recent emergence of extensively multidrug-resistant Mycobacterium tuberculosis strains has further complicated the control of tuberculosis. There is an urgent need for the development of new molecular candidates antitubercular drugs. Medicinal plants have been an excellent source of leads for the development of drugs. The aim of this study was to evaluate the in vitro activity of 28 alcoholic extracts and essential oils of native and exotic Brazilian plants against Mycobacterium tuberculosis and to further study these extracts through chemical fractionation, the isolation of their constituents, and an evaluation of the in vivo acute toxicity of the active extracts. To the best of our knowledge this is the first chemical characterization, antituberculosis activity and acute toxicity evaluation of Annona sylvatica. Methods The anti-mycobacterial activity of these extracts and their constituent compounds was evaluated using the resazurin reduction microtiter assay (REMA). To investigate the acute toxicity of these extracts in vivo, female Swiss mice were treated with the extracts at doses of 500, 1000 and 2000 mg · kg-1 of body weight. The extracts were characterized by LC-MS, and the constituents were isolated and identified by chromatographic analysis of spectroscopic data. Results Of the 28 extracts, the methanol extract obtained from the leaves of Annona sylvatica showed anti-mycobacterial activity with an minimal inhibitory concentration (MIC) of 184.33 μg/mL, and the ethyl acetate fraction (EAF) resulting from liquid-liquid partitioning of the A. sylvatica extract showed an MIC of 115.2 μg/mL. The characterization of this extract by LC-MS identified flavonoids and acetogenins as its main constituents. The phytochemical study of the A. sylvatica EAF resulted in the isolation of quercetin, luteolin, and almunequin. Conclusions Among the compounds isolated from the EAF, luteolin and almunequin were the most promising, with MICs of 236.8 μg/mL (827.28 μM) and 209.9 μg/mL (328.48 μM), respectively. The acute administration of the EAF fraction in doses of 500, 1000, and 2000 mg · kg-1 of body weight did not cause signs of toxicity in the treated animals.

O design e o produto: parâmetros projetuais para o planejamento de jogos geométricos pré-escolares

Educational games are responsible for part of the information assimilated by the child, because these games respect the individual learning time, enabling experimentation and, consequently, being an accessory to retain the acquired knowledge spontaneously. However, the analogy between stimulus and reception/result is not always the expected. On the one hand, there are the pedagogues, bearing the knowledge necessary to the children’s development. On the other hand, are the children, who cannot completely assimilate the concept, commonly because of lack of planning on the product. After all, the children become not interested in the game, without developing the expected cognitive abilities planned for the product. Whereas the importance of the games on children’s development, this work aimed to analyze and select educational games presented on the preschool, based on the language exploration from the Design’s knowledge and skills. Specifically, the research intends to investigate games targeted to stimulate geometric concepts, and so, to describe parameters that must be observed during the product planning.

Modelagem gravimétrica no Lineamento Guapiara, sul do Estado de São Paulo

The Guapiara Lineament is an important geological feature of the State of São Paulo, Brazil, associated with Mesozoic volcanism in the Paraná Basin. This paper aims at the interpretation and modeling of gravity data collected at a semi-detailed scale between Capão Bonito and Apiaí to identify the geometry of crustal partitioning along this lineament. Maps of Bouguer anomaly in the area show a remarkable positive gravimetric anomaly oriented NW-SE, which coincides with the orientation of the greater concentration of dikes associated with the Guapiara Lineament. The crustal model developed from anomalies shows a significant thinning of the crust in the area and corroborates previous studies. The crustal thinning was caused by mantle rise, reflecting the epirogenetic activity generated by the propagation of the divergent tectonic forces that occurred at the margin of the South American Plate during the Cretaceous.

Obtenção e caracterização molecular e fisiológica de plantas de soja contendo o gene AtGolS2 sob déficit hídrico

Pós-graduação em Agronomia (Genética e Melhoramento de Plantas) - FCAV

Differences in the ichthyofauna feeding habits among lateral lagoons and the river channel in a large reservoir

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Quais são os fatores que estruturam as comunidades de girinos no pantanal em diferentes escalas e sub-regiões?

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Biodiversidade dos parasitas de peixes provenientes do rio Sapucaí-Mirim, Estado de São Paulo, Brasil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Dialogismo e construção da voz autoral na escrita do texto científico de jovens pesquisadores

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Mapeamento geológico-geotécnico por meio de análise integrada no Parque Estadual do Juquery - Franco da Rocha-SP

Pós-graduação em Geociências e Meio Ambiente - IGCE

Cultivo de Eucalyptus urograndis em atmosfera enriquecida com CO2: mudanças no proteoma cloroplastidial

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Reflexões a respeito da educação a distância: a visão de professores em formação continuada

The study aimed to analyze the vision of teachers in conducting a continuing education course in level of expertise in the field of special education, focusing on Global Development Disorders, through the modality of distance education. The current study was provided by the Network Program São Paulo Teacher Training - REDEFOR in partnership with Universidade Estadual Paulista-UNESP, for teachers of the state of São Paulo. The survey went through some steps, namely: a) literature review a brief history of Distance Education in Brazil and in the world thus seeking to understand their development to the present day, including legislation that regulates; b) study and survey of potential and limiting factors for distance education in teacher training. Was observed of initial and continuing teacher education in the distance mode, focusing on education and training policies implemented by the state of São Paulo, in recent years; c) data collection with 33 teachers in the continuing education process, e-mail and performed with the use of Google Drive tool that enabled the creation of online search form; d) organization, analysis and interpretation of data and its consequences. Through the results it observed that distance education is considered a favorable mode of education for the continuing education of teachers. The course was offered via Moodle and tools were considered easy to assimilate and elaborate activities in general, favored the construction of knowledge, and some questioned for its effectiveness. It follows therefore, that distance education provides continuing education for teachers, but it is necessary in the preparation of these courses is taken into consideration the tools and resources available in their construction, contributing to a dynamic and interactive model between all participants in which the exchange of experiences, questions, doubts, can effectively contribute to the construction of knowledge and possible developments in..

Niche Specialization and Conservation Biology of Cicindela nevadica lincolniana

As with many organisms across the globe, Cicindela nevadica lincolniana is threatened with extinction. Understanding ecological factors that contribute to extinction vulnerability and what methods aid in the recovery of those species is essential in developing successful conservation programs. Here we examine behavioral mechanisms for niche partitioning along with improving techniques for captive rearing protocol and increasing public awareness about the conservation of this local insect. Ovipositional selectivity was examined for Cicindela nevadica lincolniana, Cicindela circumpicta, Cicindela togata, Cicindela punctulata, and Cicindela fulgida. Models reflect that these species of co-occurring tiger beetles select different ranges of salinity in which to oviposit thereby reducing the potential for interspecific competition. In a second study, thermoregulatory niche partitioning was examined for the same complex of tiger beetle species. Time spent in the sun, on different substrates, and engaging in various behaviors associated with thermoregulation were significantly different during different parts of the day and between species. I continued along a previous line of study to develop a viable captive rearing program. So far fourteen adult Cicindela nevadica lincolniana have been successfully reared in captivity. Overwintering mortality has been determined as a key factor in the mortality of this species in captivity. Finally, I examined the potential for using the visual arts to promote the conservation of Cicindela nevadica lincolniana and associated saline wetlands. The results from surveys conducted at the exhibit suggest that art exhibits can have a strong positive impact on members of the community.

IMMIGRATION, THE AMERICAN WEST, AND THE TWENTIETH CENTURY: GERMAN FROM RUSSIA, OMAHA INDIAN, AND VIETNAMESE-URBAN VILLAGERS IN LINCOLN, NEBRASKA

The North American West is a culturally and geographically diverse region that has long been a beacon for successive waves of human immigration and migration. A case in point, the population of Lincoln, Nebraska -- a capital city on the eastern cusp of the Great Plains -- was augmented during the twentieth century by significant influxes of Germans from Russia, Omaha Indians, and Vietnamese. Arriving in clusters beginning in 1876, 1941, and 1975 respectively, these newcomers were generally set in motion by dismal economic, social, or political situations in their sending nations. Seeking better lives, they entered a mainstream milieu dominated by native-born Americans -- most part of a lateral migration from Iowa, Illinois, and Pennsylvania -- who only established their local community in 1867. While this mainstream welcomed their labor, it often eschewed the behaviors and cultural practices ethnic peoples brought with them. Aware but not overly concerned about these prejudices, all three groups constructed or organized distinct urban villages. The physical forms of these enclaves ranged from homogeneous neighborhoods to tight assemblies of relatives, but each suited a shared preference for living among kinspeople. These urban villages also served as stable anchors for unique peoples who were intent on maintaining aspects of their imported cultural identities. Never willing to assimilate to mainstream norms, urban villagers began adapting to their new milieus. While ethnic identity constructions in Lincoln proved remarkably enduring, they were also amazingly flexible. In fact, each subject group constantly negotiated their identities in response to interactions among particular, cosmopolitan, and transnational forces. Particularism refers largely to the beliefs, behaviors, and organizational patterns urban villagers imported from their old milieus. Cosmopolitan influences emanated from outside the ethnic groups and were dictated largely but not exclusively by the mainstream. Transnationalism is best defined as persistent, intense contact across international boundaries. These influences were important as the particularism of dispersed peoples was often reinforced by contact with sending cultures. Adviser: John. R. Wunder

Reflexões a respeito da educação a distância: a visão de professores em formação continuada

The study aimed to analyze the vision of teachers in conducting a continuing education course in level of expertise in the field of special education, focusing on Global Development Disorders, through the modality of distance education. The current study was provided by the Network Program São Paulo Teacher Training - REDEFOR in partnership with Universidade Estadual Paulista-UNESP, for teachers of the state of São Paulo. The survey went through some steps, namely: a) literature review a brief history of Distance Education in Brazil and in the world thus seeking to understand their development to the present day, including legislation that regulates; b) study and survey of potential and limiting factors for distance education in teacher training. Was observed of initial and continuing teacher education in the distance mode, focusing on education and training policies implemented by the state of São Paulo, in recent years; c) data collection with 33 teachers in the continuing education process, e-mail and performed with the use of Google Drive tool that enabled the creation of online search form; d) organization, analysis and interpretation of data and its consequences. Through the results it observed that distance education is considered a favorable mode of education for the continuing education of teachers. The course was offered via Moodle and tools were considered easy to assimilate and elaborate activities in general, favored the construction of knowledge, and some questioned for its effectiveness. It follows therefore, that distance education provides continuing education for teachers, but it is necessary in the preparation of these courses is taken into consideration the tools and resources available in their construction, contributing to a dynamic and interactive model between all participants in which the exchange of experiences, questions, doubts, can effectively contribute to the construction of knowledge and possible developments in..

Estudo do meio físico e caracterização da capacidade de suporte natural da região de Pirassununga/SP

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)