Documents et pièces diverses, en majeure partie originaux, conservés jadis dans le TRESOR DES CHARTES DE LORRAINE. — Nombreux sceaux. — Voy. l'analyse détaillée de la plupart de ces pièces dans l'Inventaire de Dufourny (Bibl. nat., ms. fr. 4880-4886 ; Bibl. de Nancy, mss. 754-765). On ne relève ici que les pièces qui n'y figurent pas. XLV — Portefeuille-layette 23 b (1344-1622)

Metz évêché ; Bulles et autres pièces, n os 1-10, 12-14, 17-24, 26-36. Voy. Bibl. nat., ms. fr. 4883, pp. 7789-7800 ; Bibl. de Nancy, ms. 763, 1 re partie, pp. 167 et 168. — Voy. ms. fr. 4883, pp. 7761-7789.

Le Dieu des premiers chrétiens

Cet essai décrit les débuts du christianisme primitif à partir des divers types de langages utilisés pour exprimer et transmettre l'Evangile. Une première partie répertorie et analyse les divers moyens de communication du Nouveau Testament, tels la parabole, le récit de miracle ou les récits de résurrection. Dans la seconde partie, l'auteur aborde quelques questions plus spécifiques comme, par exemple, la position de Paul sur le rôle des femmes dans l'Eglise. Par rapport à la précédente édition, cette nouvelle version propose un chapitre supplémentaire sur l'Evangile de Jean.

Representação e propagação de incertezas em dados de solos: I - Atributos categóricos

Este trabalho teve por objetivos utilizar krigagem por indicação para espacializar propriedades de solos expressas por atributos categóricos, gerar uma representação acompanhada de medida espacial de incerteza e modelar a propagação de incerteza pela álgebra de mapas por meio de procedimentos booleanos. Foram estudados os atributos: teores de potássio (K) e de alumínio trocáveis, saturação por bases (V), soma de bases (S), capacidade de troca catiônica (CTC), textura (Tx) e classes de relevo (CR), de profundidade efetiva do solo, de drenagem interna e de pedregosidade e, ou, rochosidade, extraídos de 222 perfis pedológicos e de 219 amostras extras, referentes a solos do estado de Santa Catarina. A espacialização das incertezas evidenciou a variabilidade espacial dos dados a qual foi relacionada com a origem das amostras e com o comportamento do atributo. Os atributos S, V, K e CR apresentaram grau de incerteza maior do que o de Tx e CTC e houve aumento da incerteza quando representações categóricas foram integradas.

Representação e propagação de incertezas em dados de solo: II - Atributos numéricos

Este trabalho teve por objetivos utilizar krigagem por indicação para espacializar propriedades de solos expressas por atributos numéricos, gerar uma representação acompanhada de medida espacial de incerteza e modelar a propagação de incerteza por procedimentos fuzzy de álgebra de mapas. Foram estudados os atributos: teores de potássio (K) e de alumínio (Al) trocáveis, saturação por bases (V), soma de bases (S), capacidade de troca catiônica (CTC) e teor de areia total (AT), extraídos de 222 perfis pedológicos e de 219 amostras extras, localizados no estado de Santa Catarina. Quando os atributos foram expressos em classes de fertilidade, a incerteza de Al, S e V aumentou e a de K e CTC diminuiu, considerando intervalos de confiança de 95 % de probabilidade. Constatou-se que um maior número de dados numéricos de K, S e V levou a uma maior incerteza na inferência espacial, enquanto o maior número de dados numéricos de AT e CTC diminuiu o grau de incerteza. A incerteza diminuiu quando diferentes representações numéricas foram integradas.

Serial protein labeling with infrared maleimide dyes to identify cysteine modifications

Cysteine thiol modifications are increasingly recognized to occur under both physiological and pathophysiological conditions, making their accurate detection, identification and quantification of growing importance. However, saturation labeling of thiols with fluorescent dyes results in poor protein recuperation and therefore requires the use of large quantities of starting material. This is especially important in sequential dye-labeling steps when applied for an identification of cysteine modifications. First, we studied the effects of different detergents during labeling procedure, i.e. Tween 20, Triton X-100 and CHAPS, on protein yield and composition. Tween 20 and Triton X-100 resulted in yields of around 50% labeled proteins compared to only 10% with PBS alone and a most diversified 2-DE protein pattern. Secondly, Tween 20 was used for serial protein labeling with maleimid fluorophores, first to conjugate to accessible thiols and after a reduction to label with another fluorophore previously masked di-sulphide and/or oxidized proteins in frontal cortex autopsy tissue of a subject with mild Alzheimer's disease. Two-DE DIGE revealed a complex protein pattern of readily labeled thiols and di-sulphide and/or oxidized proteins. Seventeen proteins were identified by MALDI-TOF and by peptide fingerprints. Several proteins were oxidized and involved in Alzheimer's disease. However methionine oxidation was prevalent. Infrared DIGE may provide an additional tool for an identification of oxidation susceptible proteins.

Immunohistochemical and flow cytometric analysis of long-term label-retaining cells in the adult heart.

Cardiac-resident stem/progenitor cells have been identified based on expression of stem cell-associated antigens. However, no single surface marker allows to identify a definite cardiac stem/progenitor cell entity. Hence, functional stem cell markers have been extensively searched for. In homeostatic systems, stem cells divide infrequently and therefore retain DNA labels such as 5-bromo-2'-deoxyuridine, which are diluted with division. We used this method to analyze long-term label-retaining cells in the mouse heart after 14 days of 5-bromo-2'-deoxyuridine administration. Labeled cells were detected using immunohistochemical and flow-cytometric methods after varying chasing periods up to 12 months. Using mathematical models, the observed label dilution could consistently be described in the context of a 2-population model, whereby a population of rapidly dividing cells accounted for an accelerated early decline, and a population of slowly dividing cells accounted for decelerated dilution on longer time scales. Label-retaining cells were preferentially localized in the atria and apical region and stained negative for markers of the major cell lineages present in the heart. Most cells with long-term label-retention expressed stem cell antigen-1 (Sca-1). Sca-1(+)CD31(-) cells formed cell aggregates in culture, out of which lineage-negative (Lin(-))Sca-1(+)CD31(-) cells emerged, which could be cultured for many passages. These cells formed cardiospheres and showed differentiation potential into mesenchymal cell lineages. When cultured in cardiomyogenic differentiation medium, they expressed cardiac-specific genes. In conclusion, recognition of slow-cycling cells provides functional evidence of stem/progenitor cells in the heart. Lin(-)Sca-1(+)CD31(-) cardiac-derived progenitors have a potential for differentiation into cardiomyogenic and mesenchymal cell lineages.

Sistema radicular do capim-Marandu, considerando as combinações de doses de nitrogênio e de enxofre

Um sistema radicular bem desenvolvido na gramínea forrageira é necessário para a formação e sustentação da planta e, como conseqüência, para a boa produtividade da pastagem. Objetivou-se avaliar características radiculares da Brachiaria brizantha cv. Marandu, em resposta a combinações de doses de N e S na solução nutritiva, empregando sílica como substrato, num experimento realizado em casa de vegetação no período de setembro a dezembro de 2001. Utilizou-se o esquema fatorial 5² fracionado, com 13 combinações de N e S, em mg L-1, respectivamente: 14 e 3,2; 14 e 32; 14 e 80; 126 e 12,8; 126 e 64; 210 e 3,2; 210 e 32; 210 e 80; 336 e 12,8; 336 e 64; 462 e 3,2; 462 e 32 e 462 e 80, as quais foram distribuídas segundo o delineamento de blocos ao acaso, com quatro repetições. Foram realizados dois cortes nas plantas. Após o segundo corte, as raízes foram separadas do substrato e avaliadas. A concentração de N e os acúmulos de N e de S nas raízes do capim mostraram-se dependentes da interação entre as doses de N e S. A dose de N foi determinante para o comprimento, superfície, produção de massa seca, relação comprimento:massa seca e concentração de S no tecido das raízes da forrageira. A produção de massa seca, o comprimento, a superfície das raízes e o acúmulo total de N e de S nas plantas do capim tiveram significativas correlações entre si.

Exposition coloniale internationale de Paris. Commissariat général

Comprend : Afrique équatoriale française ; Afrique occidentale française / par Robert Delavignette ; bois gravés de Germaine Bernard ; Indochine / ouvrage publié sous la direction de M. Sylvain Lévi ; Ile de la Réunion, Côte française des Somalis, Établissements français de l'Inde ; Établissements français du Pacifique austral. Nouvelle Calédonie et dépendances. Nouvelles Hébrides, îles Wallis et Futuna. Établissements français de l'Océanie ; Guadeloupe, Guyane, Martinique, Saint-Pierre et Miquelon ; Madagascar et dépendances / par M. Delelée-Desloges ; illustrations de M. Le Scouezec ; Territoires africains sous mandat de la France. Cameroun et Togo / Victor Chazelas ; Suzanne Truitard

Exposition coloniale internationale de Paris. Commissariat général

Comprend : Afrique équatoriale française ; Afrique occidentale française / par Robert Delavignette ; bois gravés de Germaine Bernard ; Indochine / ouvrage publié sous la direction de M. Sylvain Lévi ; Ile de la Réunion, Côte française des Somalis, Établissements français de l'Inde ; Établissements français du Pacifique austral. Nouvelle Calédonie et dépendances. Nouvelles Hébrides, îles Wallis et Futuna. Établissements français de l'Océanie ; Guadeloupe, Guyane, Martinique, Saint-Pierre et Miquelon ; Madagascar et dépendances / par M. Delelée-Desloges ; illustrations de M. Le Scouezec ; Territoires africains sous mandat de la France. Cameroun et Togo / Victor Chazelas ; Suzanne Truitard

Nedd4-2 modulates renal Na+-Cl- cotransporter via the aldosterone-SGK1-Nedd4-2 pathway.

Regulation of renal Na(+) transport is essential for controlling blood pressure, as well as Na(+) and K(+) homeostasis. Aldosterone stimulates Na(+) reabsorption by the Na(+)-Cl(-) cotransporter (NCC) in the distal convoluted tubule (DCT) and by the epithelial Na(+) channel (ENaC) in the late DCT, connecting tubule, and collecting duct. Aldosterone increases ENaC expression by inhibiting the channel's ubiquitylation and degradation; aldosterone promotes serum-glucocorticoid-regulated kinase SGK1-mediated phosphorylation of the ubiquitin-protein ligase Nedd4-2 on serine 328, which prevents the Nedd4-2/ENaC interaction. It is important to note that aldosterone increases NCC protein expression by an unknown post-translational mechanism. Here, we present evidence that Nedd4-2 coimmunoprecipitated with NCC and stimulated NCC ubiquitylation at the surface of transfected HEK293 cells. In Xenopus laevis oocytes, coexpression of NCC with wild-type Nedd4-2, but not its catalytically inactive mutant, strongly decreased NCC activity and surface expression. SGK1 prevented this inhibition in a kinase-dependent manner. Furthermore, deficiency of Nedd4-2 in the renal tubules of mice and in cultured mDCT(15) cells upregulated NCC. In contrast to ENaC, Nedd4-2-mediated inhibition of NCC did not require the PY-like motif of NCC. Moreover, the mutation of Nedd4-2 at either serine 328 or 222 did not affect SGK1 action, and mutation at both sites enhanced Nedd4-2 activity and abolished SGK1-dependent inhibition. Taken together, these results suggest that aldosterone modulates NCC protein expression via a pathway involving SGK1 and Nedd4-2 and provides an explanation for the well-known aldosterone-induced increase in NCC protein expression.