Tasavallan Presidentti Urho Kekkosen virallinen vierailu Yhdysvaltoihin 23.-27.7.1970 (Tasavallan Presidentin saapumispuhe Washingtonissa 23.7.1970 = speech by the President of Finland, Urho Kekkonen upon his arrival in Washington, D.C. on July 23, 1970)

Puhe

Transcriptional activation of the GLUT2 gene by the IPF-1/STF-1/IDX-1 homeobox factor.

The homeodomain protein PDX-1, referred as IPF-1/STF-1/IDX-1, is a transcriptional factor that plays a critical role in the control of several genes expressed in the pancreatic islet. PDX-1 gene expression has been previously shown to be reduced in cultured beta-cell lines chronically exposed to high glucose concentrations. As the glucose transporter type 2 (GLUT2) gene expression is selectively decreased in the beta-pancreatic cells of experimental models of diabetes, we postulated that the loss of GLUT2 gene expression in the pancreatic islets of diabetic animals may be due to the loss of PDX-1 transacting function on the GLUT2 gene. We, therefore, investigated the potential role of PDX-1 in the transcriptional control of GLUT2. We have identified a repeat of a TAAT motif (5'-TAATA-ATAACA-3') conserved in the sequence of the human and murine GLUT2 promoters. Recombinant PDX-1 binds to this GLUT2TAAT motif in electrophoretic mobility shift experiments. PDX-1 antiserum detects the formation of the complex of PDX-1 with the GLUT2TAAT motif in nuclear extracts from the pancreatic insulin-secreting cell line, beta TC3. The GLUT2TAAT motif was mutated in the murine GLUT2 promoter (-1308/+49 bp) linked to a luciferase reporter gene and transfected into beta TC3 cells. Compared with the transcriptional activity of the wild type promoter, that of the mutated promoter decreases by 41%. Multiple copies of the GLUT2TAAT motif were ligated 5' to a heterologous promoter and transfected into a PDX-1-expressing cell line (beta TC3) and into cell lines lacking the homeobox factor (InR1-G9 and JEG-3). The GLUT2TAAT motif mediates the activation of the heterologous promoter in the PDX-1-expressing cell line but not in InR1-G9 or JEG-3 cell lines. Furthermore, cotransfection in a PDX-1-deficient cell line with the expression vector encoding PDX-1 transactivates specifically the heterologous promoter containing the multimerized GLUT2TAAT motif. These data demonstrate that the murine GLUT2 promoter is controlled by the PDX-1 homeobox factor through the identified GLUT2TAAT motif.

Resumen de los trabajos científicos pesqueros de la segunda expedición del BEC Profesor Mesiatzev en las aguas del Océano Pacífico adyacentes a la costa del Perú, 9 marzo al 27 junio 1973

Presenta las características de los trabajos experimentales desarollados con el fin de determinar la magnitud posible de captura de peces con el equipo hidroacústico, arrastres de comprobación y la pesca a nivel industrial.

Séptimo crucero del BEC Professor Mesiatsev. 6 mayo al 27 Junio 1973 (Crucero 7305). Imarpe-Vniro. Informe sobre los trabajos científicos pesquero-oceanográficos realizados en el Otoño de 1973, en las aguas del Oceano Pacífico adyacente a la costa del Perú

Brinda información sobre el levantamiento oceanológico completo de toda la zona peruana del Oceano Pacífico. El levantamiento se realizó en 17 secciones y en aquellos lugares donde las profundidades y el relieve del fondo lo permitieron, los trabajos en estación se acompañaron de arrastres de fondo con media hora de duración.

Quinto crucero del barco de exploración científica Profesor Mesiatsev (Vniro) (9-27 Marzo 1973). Informe sobre los trabajos científico-pesqueros en las aguas adyacentes a la costa del Perú

Brinda información sobre los resultados del crucero realizado el 6 al 29 de marzo en el Callao. Los objetivos fueron además de la pesca y su congelación, la detección de las concentraciones de peces con la ayuda de ecosondas y arrastres de prueba, se tomo muestras del fondo y del agua, colección de peces y elaboración de muestras en conserva para consumo humano.

Statsminister Kalevi Sorsa 27.1.1973

Kirje

Iowa Motorcycle Operator's Manual, April 27, 2009

The rules and regulations for owning and operating a motorcycle in Iowa

Distribución de los recursos pelágicos del 25 al 27 octubre 1999. Crucero BIC Jose Olaya Balandra 9910

Mediante el método hidroacústico se estudiaron la vinciguerria, achoveta, jurel, caballa y múnida.

Genetic and environmental effects on the covariation betweencolour polymorphism and a life history trait

Variation in coloration with a strong underlying genetic basis is frequently found within animal populations but little is known about its function. Covariation between colour polymorphism and life-history traits can arise because morphs perform differently among environments or because they possess alternative alleles coding for key life-history traits. To test these two hypotheses, we studied a population of tawny owls Strix aluco, a bird displaying red, brown and grey morphs. We assessed the colour morph of breeding females, swapped eggs or hatchlings between pairs of nests, and examined how body condition in 3-week-old nestlings covaries with coloration of foster and genetic mothers. Redder foster and genetic mothers produced young in better condition. Because in two other years we observed that greyish females produced offspring in better condition than those of red females, the present study suggests that colour polymorphism signals genetic and phenotypic adaptations to cope with a fluctuating environment.

Kajaanissa 21/6 -27

Kirje 21.6.1927

Footnotes, January-February 2003, Vol.27, no.1/2

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