Diagnostic potential of neutrophil elastase inhibitor complex in the routine care of critically ill newborn infants.

It has been suggested that determination of the neutrophil elastase alpha1-proteinase inhibitor complex (E-alpha1PI) improves the diagnosis of bacterial infection in newborns. We evaluated the use of E-alpha1PI measurements in 143 newborns, consecutively admitted to a tertiary intensive care unit, employing a new random access assay and a sampling procedure that minimises post-collection artefacts. The 95% range for noninfected newborns was 20-110 microg/l up to the 5th day of life and 20-85 microg/l thereafter. The sensitivity as to the diagnosis of culture-proven bloodstream infection was 80% for E-alpha1PI, 86% for the immature to total neutrophil ratio, 64% for C-reactive protein and 37% for the total white blood cell count. The corresponding specificity amounted to 97%, 85%, 85% and 86%, respectively. E-alpha1PI increases preceded elevations of C-reactive protein by 18 h. Like C-reactive protein, E-alpha1PI levels did not distinguish between bloodstream infection and non-bacterial inflammatory responses. Results of E-alpha1PI became available within 1 h of collection and usually 2-3 h before manual leucocyte counts. CONCLUSION: Determination of neutrophil elastase alpha1-proteinase inhibitor levels yields diagnostic advantages comparable to those of manual differential counts but provide faster turnaround times.

Location-independent and location-linked representations of sound objects.

For the recognition of sounds to benefit perception and action, their neural representations should also encode their current spatial position and their changes in position over time. The dual-stream model of auditory processing postulates separate (albeit interacting) processing streams for sound meaning and for sound location. Using a repetition priming paradigm in conjunction with distributed source modeling of auditory evoked potentials, we determined how individual sound objects are represented within these streams. Changes in perceived location were induced by interaural intensity differences, and sound location was either held constant or shifted across initial and repeated presentations (from one hemispace to the other in the main experiment or between locations within the right hemispace in a follow-up experiment). Location-linked representations were characterized by differences in priming effects between pairs presented to the same vs. different simulated lateralizations. These effects were significant at 20-39 ms post-stimulus onset within a cluster on the posterior part of the left superior and middle temporal gyri; and at 143-162 ms within a cluster on the left inferior and middle frontal gyri. Location-independent representations were characterized by a difference between initial and repeated presentations, independently of whether or not their simulated lateralization was held constant across repetitions. This effect was significant at 42-63 ms within three clusters on the right temporo-frontal region; and at 165-215 ms in a large cluster on the left temporo-parietal convexity. Our results reveal two varieties of representations of sound objects within the ventral/What stream: one location-independent, as initially postulated in the dual-stream model, and the other location-linked.

Règle de s. Augustin, suivie de constitutions et de pièces diverses se rapportant à la congrégation de Windesheim.

Abbé de Tersan (?); — abbaye de Saint-Jean de Rebdorf en Bavière d'après Ruf, Rebdorf, 261; Krämer, Rebdorf, 672

Histoire du monastère de Selby, dépendance de Saint-Germain d'Auxerre.

Donné par Châtre de Cangé en 1733, après la vente de ses manuscrits à la Bibliothèque du roi; cf. Catalogue des livres du Cabinet de M..., Paris, 1733, description à la main ajoutée sur les derniers feuillets de garde de plusieurs exemplaires de ce catalogue "Notice de quelques manuscrits d'elite qui n'étoient pas compris dans le catalogue et que M. du Cangé a donnés au roi... Histoire du monastère de Selby, ordre de St Benoît, dans la province d'Yorc. Ce vol. étoit sans inscription et sans n° et vient pourtant du fond de du Cangé"; Delisle, Cab. des mss., I, 411-412; n° 143 de la liste des manuscrits de Cangé établie par Méon; — ex-libris (XVIIe s.)"Monasterii S. Germain Autisiodorensis" de l'abbaye de Saint-Germain d'Auxerre (1), cf. Delisle, Cab. des mss., II, 405.

Actes relatifs au collège de Navarre.

Acquis le 3 août 1824 de M. de Bure, libraire, suite à la vente Chardin, n° 511, pour un prix non précisé; cf. B.n.F., département des Manuscrits, Archives Modernes 492ter, registre des acquisitions du département des Manuscrits 1821-1830, f. 142-143 "Statuts donnés par la reine Jeanne de Navarre femme du roi Philippe-le-Bel, au collège royal de Champagne, dit de Navarre, dont elle étoit fondatrice; ms. sur velin in 4°, relié proprement".

Marbles and stones of Hispania : exhibition catalogue = Marbres i pedres d'Hispania : catàleg de l'exposició = Mármoles y piedras de Hispania : catálogo de la exposición

Primera guia de materials lapidis hispànics explotats en època romana a la península Ibèrica. El treball recull 16 varietats, i té especial interès en la descripció de la geologia dels entorns de les explotacions. Entre els materials lapidis figuren, per exemple, marbres de la zona d’Estremoz (Portugal), broccatello (també anomanat jaspi de la Cinta, procedent de Tortosa), marbres de la zona de Macael (Andalusia) i la pedra de Buixcarró (València). També s'hi estudien amb detall els punts d’explotació (pedreres) i les diverses aplicacions donades a cada tipus de pedra. El llibre és trilingüe (anglès, català i castellà), està molt ben il·lustrat (en color) i s'ha publicat com a catàleg ampliat d'una exposició amb el mateix títol muntada en el marc de la IX Asmosia Internacional Conference (Tarragona, juny del 2009).

Produtividade e rendimento do cafeeiro submetido a lâminas de irrigação

O objetivo deste trabalho foi avaliar a produtividade e o rendimento das quatro primeiras safras de cafeeiro (Coffea arabica L.), cultivado sob lâminas de irrigação. O experimento foi conduzido na Fazenda Experimental do Glória, Uberlândia, MG. O delineamento experimental foi o de blocos ao acaso, com quatro repetições e oito tratamentos constituídos das lâminas de irrigação equivalentes a 0 (sem irrigação), 30, 60, 90, 120, 150, 180 e 210% da evaporação em tanque Classe A (ECA). O plantio foi realizado em janeiro de 2001, no espaçamento de 3,5x0,7 m. As parcelas foram constituídas por três linhas com oito plantas cada. Foi utilizado o sistema de irrigação por gotejamento, com emissores autocompensantes de vazão 3,5 L h-1. A produtividade máxima foi de 115 sacas ha-1, obtida com a lâmina de 164,1% da ECA. A reposição de 143% da ECA propiciou rendimento ótimo de 291,8 L de "café da roça" por saca beneficiada. O efeito das lâminas de irrigação sobre a produtividade e o rendimento depende do ano. A irrigação não ameniza a bienalidade da produção do cafeeiro, em relação às plantas cultivadas em sequeiro.

New microsatellite markers developed from an enriched microsatellite common bean library

The objective of this work was to develop new microsatellite markers in common bean. Ninety nine new microsatelitte loci were developed from a microsatellite enriched library for (CT)8 and (GT)8 motifs, from CAL-143 line. The majority of microsatellite sequences (51%) was related to cellular metabolism. The remaining sequences were associated to transcription functions. Only 17.2% of the sequences presented some level of similarity with other plant species genes.

V3 net charge: additional tool in HIV-1 tropism prediction.

Genotype-based algorithms are valuable tools for the identification of patients eligible for CCR5 inhibitors administration in clinical practice. Among the available methods, geno2pheno[coreceptor] (G2P) is the most used online tool for tropism prediction. This study was conceived to assess if the combination of G2P prediction with V3 peptide net charge (NC) value could improve the accuracy of tropism prediction. A total of 172 V3 bulk sequences from 143 patients were analyzed by G2P and NC values. A phenotypic assay was performed by cloning the complete env gene and tropism determination was assessed on U87_CCR5(+)/CXCR4(+) cells. Sequences were stratified according to the agreement between NC values and G2P results. Of sequences predicted as X4 by G2P, 61% showed NC values higher than 5; similarly, 76% of sequences predicted as R5 by G2P had NC values below 4. Sequences with NC values between 4 and 5 were associated with different G2P predictions: 65% of samples were predicted as R5-tropic and 35% of sequences as X4-tropic. Sequences identified as X4 by NC value had at least one positive residue at positions known to be involved in tropism prediction and positive residues in position 32. These data supported the hypothesis that NC values between 4 and 5 could be associated with the presence of dual/mixed-tropic (DM) variants. The phenotypic assay performed on a subset of sequences confirmed the tropism prediction for concordant sequences and showed that NC values between 4 and 5 are associated with DM tropism. These results suggest that the combination of G2P and NC could increase the accuracy of tropism prediction. A more reliable identification of X4 variants would be useful for better selecting candidates for Maraviroc (MVC) administration, but also as a predictive marker in coreceptor switching, strongly associated with the phase of infection.