Robust Bayesian analysis of heavy-tailed stochastic volatility models using scale mixtures of normal distributions

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Determination of the local site occupancy of Eu3+ ions in ZnAl2O4 nanocrystalline powders

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Influência dos períodos, seco e chuvoso, sobre os parâmetros clínicos em caprinos das raças Canindé e Moxotó

Os caprinos têm um sistema de termorregulação que visa manter a temperatura corporal constante dentro de certos limites, independente da temperatura ambiente. Quando esses limites são estabelecidos, eles são usados em diversos mecanismos fisiológicos para manter a temperatura corporal (TC) dentro dos limites normais. Assim, a produção animal pode diminuir devido ao estresse térmico causado por temperaturas elevadas, especialmente em áreas semi-áridas como o Nordeste do Brasil. Este trabalho teve como objetivo avaliar o comportamento fisiológico de diferentes grupos genéticos de caprinos nativos de acordo com a resposta fisiológica das varáveis temperatura retal (TR), frequência respiratória (FR), batimentos cardíacos (BC) e movimentos ruminais (MR), e estabelecer os parâmetros fisiológicos para estas raças sob as condições do semiárido do Nordeste do Brasil. Foram selecionados aleatoriamente 30 animais, sendo cinco machos e dez fêmeas da raça Canindé e cinco machos e dez fêmeas da raça Moxotó, dos quais foram aferidos as variáveis BC, FR e TR nos meses de abril e setembro/2007 e 2008. Observou-se influência significativa (P<0,05) do período do ano no BC, na FR e na TR na raça Canindé, sendo observados valores mais elevados de BC e TR no período chuvoso. Na raça Moxotó observou-se uma diferença significativa (P<0,05) quanto ao BC e TR entre os períodos estudados, sendo observadas na estação seca maiores valores para estas variáveis. Também se observou nesta raça, uma correlação negativa (47%) entre a temperatura ambiente (TA) e TR no período seco, enquanto que no período chuvoso, essa correlação foi positiva (28%). A correlação entre TA e BC foi negativa (21%) no período chuvoso e, positiva (25%) no período seco. Na raça Canindé observou-se correlação negativa (39%) entre TA e movimento ruminal (MR) na época chuvosa, enquanto que no período seco, essa correlação foi positiva (33%). Os parâmetros clínicos avaliados neste estudo encontram-se dentro da normalidade para a espécie caprina no semi-árido nordestino e a raça Moxotó apresentou comportamento condizente com uma maior tolerância ao clima da região e um maior grau de adaptabilidade.

The implications of microsurgical anatomy for surgical approaches to the sellar region

The knowledge of the normal anatomy and variations regarding the management of tumors of the sellar region is paramount to perform safe surgical procedures. The sellar region is located in the center of the middle cranial fossa; it contains complex anatomical structures, and is the site of various pathological processes: tumor, vascular, developmental, and neuroendocrine. We review the microsurgical anatomy (microscopic and endoscopic) of this region and discuss the surgical nuances regarding this topic, based on anatomical concepts.

Histopathological changes induced by extracts from the tissue covering the stingers of Potamotrygon falkneri freshwater stingrays

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Applicability of the equations of Freundlich and Langmuir to the adsorption of the azo dye procion scarlet on paramorphic colonies of Neurospora crassa.

Experiments on the adsorption of Procion Scarlet MX-G by normal hyphae and by paramorphic colonies of Neurospora crassa were performed at pH 2.5, 4.5 and 6.5 at 30 degrees C. The measured adsorption isotherms were evaluated by the Freundlich and Langmuir equations. The removal of dye was most effective at pH 2.5 and more dye was adsorbed per unit mass of cells in the paramorphic cultures than in the normal hyphae. The statistical tests showed Langmuir's equation to give a better fit to the adsorption data.

Rho-omega mixing and neutron-proton self-energies in the Walecka model

We use the Walecka model to investigate the influence of the charge symmetry breaking ρ0-ω mixing interaction on the neutron-proton self-energy difference in nuclear matter. Using 2mρ〈ρ0|H|ω〉 = -4500 MeV2, and employing the Dirac-Hartree-Fock approximation, we find that the neutron-proton self-energy difference is a decreasing function of the nuclear matter density, and that it has a value of the order of 700 keV at the normal density. The results indicate that the Nolen-Schiffer anomaly might be explained by means of relativistic nuclear models in a similar way as it is explained by means of non-relativistic models.

Partial purification and characterization of lysine-ketoglutarate reductase in normal and opaque-2 maize endosperms

Lysine-ketoglutaratc reductase catalyzes the first step of lysine catabolism in maize (Zea mays L.) endosperm. The enzyme condenses L-lysine and α-ketoglutarate into saccharopine using NADPH as cofactor. It is endosperm-specific and has a temporal pattern of activity, increasing with the onset of kernel development, reaching a peak 20 to 25 days after pollination, and thereafter decreasing as the kernel approaches maturity. The enzyme was extracted from the developing maize endosperm and partially purified by ammonium-sulfate precipitation, anion-exchange chromatography on DEAE-cellulose, and affinity chromatography on Blue-Sepharose CL-6B. The preparation obtained from affinity chromatography was enriched 275-fold and had a specific activity of 411 nanomoles per minute per milligram protein. The native and denaturated enzyme is a 140 kilodalton protein as determined by polyacrylamide gel electrophoresis. The enzyme showed specificity for its substrates and was not inhibited by either aminoethyl-cysteine or glutamate. Steady-state product-inhibition studies revealed that saccharopine was a noncompetitive inhibitor with respect to α-ketoglutarate and a competitive inhibitor with respect to lysine. This is suggestive of a rapid equilibriumordered binding mechanism with a binding order of lysine, α-ketoglutarate, NADPH. The enzyme activity was investigated in two maize inbred lines with homozygous normal and opaque-2 endosperms. The pattern of lysine-ketoglutarate reductase activity is coordinated with the rate of zein accumulation during endosperm development. A coordinated regulation of enzyme activity and zein accumulation was observed in the opaque-2 endosperm as the activity and zein levels were two to three times lower than in the normal endosperm. Enzyme extracted from L1038 normal and opaque-2 20 days after pollination was partially purified by DEAE-cellulose chromatography. Both genotypes showed a similar elution pattern with a single activity peak eluted at approximately 0.2 molar KCL. The molecular weight and physical properties of the normal and opaque-2 enzymes were essentially the same. We suggest that the Opaque-2 gene, which is a transactivator of the 22 kilodalton zein genes, may be involved in the regulation of the lysine-ketoglutarate reductase gene in maize endosperm. In addition, the decreased reductase activity caused by the opaque-2 mutation may explain, at least in part, the elevated concentration of lysine found in the opaque-2 endosperm.

Loss of space and dental arch length after the loss of the lower first primary molar: A longitudinal study

The premature loss of primary teeth may harm the normal occlusal development, although there are debates relating to the necessity of using space maintainer appliances. The aim of the study is to evaluate the changes in the dental arch perimeter and the space reduction after the premature loss of the lower first primary molar in the mixed dentition stage. The sample consists of 4 lower arch plaster models of 31 patients, within the period of pre-extraction, 6, 12 and 18 months after the lower first primary molar extraction. A reduction of space was noted with the cuspid dislocation and the permanent incisors moving toward the space of the extraction site. It was concluded that the lower first molar primary premature loss, during the mixed dentition, implicates an immediate placement of a space maintainer.

Tectonics and paleogeography along the Amazon river

The main structural and geomorphological features along the Amazon River are closely associated with Mesozoic and Cenozoic tectonic events. The Mesozoic tectonic setting is characterised by the Amazonas and Marajó Basins, two distinct extensional segments. The Amazonas Basin is formed by NNE-SSW normal faults, which control the emplacement of dolerite dykes and deposition of the sedimentary pile. In the more intense tectonic phase (mid-Late Cretaceous), the depocentres were filled with fluvial sequences associated with axial drainage systems, which diverge from the Lower Tapajós Arch. During the next subsidence phase, probably in the Early Tertiary, and under low rate extension, much of the drainage systems reversed, directing the paleo-Amazon River to flow eastwards. The Marajó Basin encompasses NW-SE normal faults and NE-SW strike-slip faults, with the latter running almost parallel to the extensional axes. The normal faults controlled the deposition of thick rift and post-rift sequences and the emplacement of dolerite dykes. During the evolution of the basin, the shoulder (Gurupá Arch) became distinct, having been modelled by drainage systems strongly controlled by the trend of the strike-slip faults. The Arari Lineament, which marks the northwest boundary of the Marajó Basin, has been working as a linkage corridor between the paleo and modern Amazon River with the Atlantic Ocean. The neotectonic evolution since the Miocene comprises two sets of structural and geomorphological features. The older set (Miocene-Pliocene) encompasses two NE-trending transpressive domains and one NW-trending transtensive domain, which are linked to E-W and NE-SW right-lateral strike-slip systems. The transpressive domains display aligned hills controlled by reverse faults and folds, and are separated by large plains associated with pull-apart basins along clockwise strike-slip systems (e.g. Tupinambarana Lineament). Many changes were introduced in the landscape by the transpressive and transtensive structures, such as the blockage of major rivers, which evolved to river-lakes, transgression of the sea over a large area in the Marajó region, and uplift of long and narrow blocks that are oblique to the trend of the main channel. The younger set (Pliocene-Holocene) refers to two triple-arm systems of rift/rift/strike-slip and strike-slip/strike-slip/rift types, and two large transtensive segments, which have controlled the orientation of the modern drainage patterns. © 2001 Elsevier Science Ltd. All rights reserved.

In vitro evaluation of the antimicrobial activity of a castor oil-based irrigant

The antimicrobial activity of irrigating solutions - Endoquil (castor oil detergent), 2% chlorhexidine gluconate solution, and 0.5% NaOCI solution - was evaluated against Gram-positive cocci (Micrococcus luteus, Staphylococcus aureus, Enterococcus faecalis, Staphylococcus epidermidis, Streptococcus mutans, and Streptococcus sobrinus), Gramnegative rods (Escherichia coli and Pseudomonas aeruginosa), and the yeast Candida albicans. Activity was evaluated using the two-layer agar diffusion technique. The base layer was obtained by pouring 10.0 ml of Muller Hinton Medium or 10.0 ml of Brain Heart Infusion agar in a Petri dish. After solidification a 5.0 ml seed layer of Muller Hinton Medium or Brain Heart Infusion agar with inoculum (106/ml) was added. Absorbent paper disks (6.0 mm in diameter) immersed in the solutions were placed at equidistant points. Plates were maintained at room temperature for 2 h for prediffusion of the solutions and incubated at 37°C for 24 h. The candle jar system was used for the Brain Heart Infusion agar plates. All tests were performed in duplicate. After incubation the medium was optimized with 0.05 g% triphenyltetrazolium chlorate gel and inhibition halos were measured. All bacterial strains were inhibited by 2.0% chlorhexidine gluconate. Endoquil was effective against Grampositive microorganisms, and 0.5% NaOCI was effective only against S. aureus. Copyright © 2001 by The American Association of Endodontists.

Assignment of the bisulfide bridges in bothropstoxin-I, a Myonecrotic Lys49 PLA2 homolog from bothrops jararacussu snake venom

Bothropstoxin-I (BthTX-I), a Lys49 phospholipase A2 homolog with no apparent catalytic activity, was first isolated from Bothrops jararacussu snake venom and completely sequenced in this laboratory. It is a 121-amino-acid single polypeptide chain, highly myonecrotic, despite its inability to catalyze hydrolysis of egg yolk phospholipids, and has 14 half-cystine residues identified at positions 27, 29, 44, 45, 50, 51, 61, 84, 91, 96, 98, 105, 123, and 131 (numbering according to the conventional alignment including gaps, so that the last residue is Cys 131). In order to access its seven disulfide bridges, two strategies were followed: (1) Sequencing of isolated peptides from (tryptic + SV8) and chymotryptic digests by Edman-dansyl degradation; (2) crystallization of the protein and determination of the crystal structure so that at least two additional disulfide bridges could be identified in the final electron density map. Identification of the disulfide-containing peptides from the enzymatic digests was achieved following the disappearance of the original peptides from the HPLC profile after reduction and carboxymethylation of the digest. Following this procedure, four bridges were initially identified from the tryptic and SV8 digests: Cys50-Cysl31, Cys51-Cys98, Cys61-Cys91, and Cys84-Cys96. From the chymotryptic digest other peptides were isolated either containing some of the above bridges, therefore confirming the results from the tryptic digest, or presenting a new bond between Cys27 and Cys 123. The two remaining bridges were identified as Cys29-Cys45 and Cys44-Cysl05 by determination of the crystal structure, showing that BthTX-I disulfide bonds follow the normal pattern of group II PLA2s. © 2001 Plenum Publishing Corporation.

The origin of lipid droplets in the post-pharyngeal gland of Dinoponera australis (Formicidae: Ponerinae)

The post-pharyngeal gland of normal and starvation ants was studied under TEM. This study showed an orgin of lipids droplets from mitochondria (named derivate mitochondria) in the normal ants and the lipids absence or reduction, in fasting ants.

Physiological and cellular responses in two populations of the mussel Perna perna collected at different sites from the Coast of São Paulo, Brazil

The physiological conditions of mussels from Ubatuba and Santos and also of organisms transplanted from Ubatuba to Santos were studied by using different techniques. Assays for lysosomal stability were conducted on the haemolymph. Heart rate activity was monitored for 6h. The embryonic development of larvae obtained from the collected mussels was analysed. For all the compared groups of mussels, no significant differences were observed for the cardiac activity monitoring and the embryonic bioassays. The mean Neutral Red (NR) retention time was similar for the animals from Santos and Ubatuba, whereas the organisms transplanted to Santos showed a reduction in the retention time of the dye, indicating damage in the lysosomal membranes. These differences were possibly due to environmental factors, but further investigations are required to confirm this hypothesis.

Corneal endothelium of the Magellanic penguin (Spheniscus magellanicus) by scanning electron microscopy

The corneal endothelium is essential for the maintenance of the corneal transparency. The aim of this study was to examine the morphology of the endothelial surface and perform morphometric analysis of the normal corneal endothelial cells of the Magellanic penguin (Spheniscus magellanicus) using scanning electron microscopy. The present work demonstrates that the corneal endothelium of the Magellanic penguin is similar to those described in other vertebrates. Copyright 2005 by American Association of Zoo Veterinarians.