Chromosome aberration and micronucleus frequencies in Allium cepa cells exposed to petroleum polluted water - A case study

In the present study, we applied Chromosome Aberration (CA) and Micronucleus (MN) tests to Allium cepa root cells, in order to evaluate the water quality of Guaeca river. This river, located in the city of Sao Sebastiao, SP, Brazil, had been affected by an oil pipeline leak. Chemical analyses of Total Petroleum Hydrocarbons (TPHs) and Polycyclic Aromatic Hydrocarbons (PAHs) were also carried out in water samples, collected in July 2005 (dry season) and February 2006 (rainy season) in 4 different river sites. The largest CA and MN incidence in the meristematic cells of A. cepa was observed after exposure to water sample collected during the dry season, at the spring of the river, where the oil leak has arisen. The F, cells from roots exposed to such sample (non-merismatic region) were also analyzed for the incidence of MN, showing a larger frequency of irregularities, indicating a possible development of CA into MN. Lastly, our study reveals a direct correlation between water chemical analyses (contamination by TPHs and PAHs) and both genotoxic and mutagenic effects observed in exposed A. cepa cells. (C) 2007 Elsevier B.V. All rights reserved.

Leak detection in petroleum pipelines using a fuzzy system

A methodology for pipeline leakage detection using a combination of clustering and classification tools for fault detection is presented here. A fuzzy system is used to classify the running mode and identify the operational and process transients. The relationship between these transients and the mass balance deviation are discussed. This strategy allows for better identification of the leakage because the thresholds are adjusted by the fuzzy system as a function of the running mode and the classified transient level. The fuzzy system is initially off-line trained with a modified data set including simulated leakages. The methodology is applied to a small-scale LPG pipeline monitoring case where portability, robustness and reliability are amongst the most important criteria for the detection system. The results are very encouraging with relatively low levels of false alarms, obtaining increased leakage detection with low computational costs. (c) 2005 Elsevier B.V. All rights reserved.

Comparative sediment quality assessment in different littoral ecosystems from Spain (Gulf of Cadiz) and Brazil (Santos and Sao Vicente estuarine system)

The goal of this work was to establish comparisons among environmental degradation in different areas from Southern Spain (Gulf of Cadiz) and Brazil (Santos and Sao Vicente estuary), by using principal component analyses (PCA) to integrate sediment toxicity (amphipods mortality) and chemical-physical data (Zn, Cd, Pb; Cu, Ni, Co, V, PCBs, PAHs concentrations, OC and fines contents). The results of PCA extraction of Spanish data showed that Bay of Cadiz, CA-1 did not present contamination or degradation; CA-2 exhibited contamination by PCBs, however it was not related to the amphipods mortality. Ria of Huelva was the most impacted site, showing contamination caused principally by hydrocarbons, in HV-1 and HV-2, but heavy metals were also important contaminants at HV-1, HV-2 and HV-3. Algeciras Bay was considered as not degraded in GR-3 and -4, but in GR-3' high contamination by PAHs was found. In the Brazilian area, the most degraded sediments were found in the stations situated at the inner parts of the estuary (SSV-2, SSV-3, and SSV-4), followed by SSV-6, which is close to the Submarine Sewage Outfall of Santos - SSOS. Sediments from SSV-1 and SSV-5 did not present chemical contamination, organic contamination or significant amphipod mortality. The results, of this investigation showed that both countries present environmental degradation related to PAHs: in Spain, at Ria of Huelva and Gudarranque river's estuary areas; and in Brasil, in the internal portion of the Santos and Sao Vicente estuary. The same situation is found for heavy metals, since all of the identified metals are related to toxicity in the studied areas, with few exceptions (V for both Brazil and Spain, and Cd and Co for Brazilian areas). The contamination by PCBs is more serious for Santos and Sao Vicente estuary than for the investigated areas in Gulf of Cadiz, where such compound did not relate to the toxicity. (c) 2006 Elsevier Ltd. All rights reserved.

Optimum design of microirrigation systems in sloping lands

When an area to be irrigated has a high slope gradient in the manifold line direction, an option is to use a tapered pipeline to economize on pipe costs and to keep pressure head variations within desired limits. The objective of this paper is to develop a linear optimization model to design a microirrigation system with tapered, downhill manifold lines, minimizing the equivalent annual cost of the hydraulic network and the annual pumping cost, and maximizing the emission uniformity previously established to the subunit. The input data are irrigation system layout, cost of all hydraulic network components, and electricity price. The output data are equivalent annual cost, pipeline diameter in each line of the system, pressure head in each node, and total operating pressure head. To illustrate its capability, the model is applied in a citrus orchard in Sao, Paulo State, Brazil, considering slopes of 3, 6, and 9%. The model proved to be efficient in the design of the irrigation system in terms of the emission uniformity desired.

Anomalous flow behaviour of bauxite tailings

This paper deals with the anomalous flow behaviour observed in two bauxite tailings pumping systems, with 450 mm and 680 mm outer diameter. In order to enlarge the pipeline lengths in the field, tests were carried out in a laboratory test-loop in order to try to understand the anomalous (intermittent) flow behaviour and to solve the problem. Based on data obtained from these laboratory tests and using a generalized REYNOLDS number it was possible to obtain results that fit the MOODY ROUSE diagram.

Design of microirrigation systems in sloping lands using linear programming

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Um estudo sobre o osu do modelo de cor RGB no proceso de correspondência por correlação

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

A multiagent architecture for supervisory and control system

Supervising and controlling the many processes involved in petroleum production is both dangerous and complex. Herein, we propose a multiagent supervisory and control system for handle continuous processes like those in chemical and petroleum industries In its architeture, there are agents responsible for managing data production and analysis, and also the production equipments. Fuzzy controllers were used as control agents. The application of a fuzzy control system to managing an off-shore installation for petroleum production onto a submarine separation process is described. © 2008 IEEE.

Fatigue apparatus for geometrically complex non-standardized specimens

Standard Test Methods (e.g. ASTM, DIN) for materials characterization in general, and for fatigue in particular, do not contemplate specimens with complex geometries, as well as the combination of axial and in-plane bending loads in their methodologies. The present study refers to some patents and the new configuration or configurations of specimens (non-standardized by the status quo of test methods) and a device developed to induce axial and bending combined forces resultants from axial loads applied by any one test equipment (dynamic or monotonic) which possesses such limitation, towards obtaining more realistic results on the fatigue behavior, or even basic mechanical properties, from geometrically complex structures. Motivated by a specific and geometrically complex aeronautic structure (motor-cradle), non-standardized welded tubular specimens made from AISI 4130 steel were fatigue-tested at room temperature, by using a constant amplitude sinusoidal load of 20 Hz frequency, load ratio R = 0.1 with and without the above referred auxiliary fatigue apparatus. The results showed the fatigue apparatus was efficient for introducing higher stress concentration factor at the welded specimen joints, consequently reducing the fatigue strength when compared to other conditions. From the obtained results it is possible to infer that with small modifications the proposed apparatus will be capable to test a great variety of specimen configurations such as: squared tubes and plates with welded or melted junctions, as well as other materials such as aluminum, titanium, composites, polymeric, plastics, etc. © 2009 Bentham Science Publishers Ltd.

Mapeamento geoambiental como subsídio na determinação de áreas de instabilidades da faixa de dutos Osvat/Osplan - São Sebastião, SP

Works of linear engineering such as roads, pipelines and transmission lines have specific mapping due to their large scale impact on the environment, thus requiring mapping methods that are both efficient and of low cost. This paper presents a proposal of Geoenvironmental mapping for works linear. The work chosen for the implementation of the method was the Osvat/Osplan pipeline located in the cities of São Sebastião and Caraguatatuba. The geoenvironment mapping was elaborate trough photo-interpretation of images of the ETM+/Landsat-7 sensor and analysis of the drainage network, thus resulting in the partitioning of the geoenvironmental units and the fracture area (structural lineaments and lines of strikes), these maps were subsequently integrated into a product called Map of environmental susceptibility to gravitational and erosive processes, which helped define the areas with potential geotechnical problems that could damage both the pipeline and the environment.

Análise de fraturamentos para determinação de áreas instáveis na faixa de dutos Osvat/Osplan - São Sebastião, em São Paulo

The present work aimed at characterizing geological features that identify areas with high ruptibility (fracturing) in the Osvat/Osplan pipeline in São Sebastião, São Paulo. The analysis of ruptile geological structures (lines of strikes and structural lineaments) through the use of orbital remote sensing was used as systematic mapping. The analysis of these features enables the inference of factors, such as permeability, infiltration and degree of shear in the region, factors which influence the processes of erosion and landslides in the area. On the map of structural lineaments, points of lineaments intersection from different directions were analyzed, followed by the counting of the frequency of these items per unit area, allowing the statistical modeling of spatial distribution, generating the map of density of structural lineament intersections, which allows determining areas with the highest percolation of fluid in the rock structure. However, on the map of lines of strikes, a space analysis was conducted to identify the two directions with higher frequency of lines of strikes in order to establish the maximums 1 and 2 and to identify the areas of abrupt changes of direction of these strike lines. In such areas where abrupt changes of directions of maximum lines of strikes occur, consequently there will be intense percolation of fluids, responsible for higher alterability of the rock/soil complex, facilitating the installation of erosion processes and landslides, increasing the area instability and consequently the vulnerability of the pipeline.

Efeitos ambientais da disposição oceânica de esgotos por meio de emissários submarinos: Uma revisão

Oceanic disposal systems of domestic sewage, or submarine sewage outfalls, have been historically used around the world as a solution for urban effluents, in special due to economic aspects. However, release into the ocean, in shallow or deep waters, may induce a set of negative environmental impacts, as eutrophication, toxic algal blooms, pathogenic microorganisms introduction and contamination by chemical substances which are capable of causing toxic effects to the biota and bioaccumulation. Thus, the release of untreated sewage into the sea does not constitute an adequate environmental practice; then, urban effluents should be treated in order to remove nutrients, contaminants and pathogenic organisms and avoid environmental degradation.

Automatic landslide recognition through Optimum-Path Forest

In this paper we shed light over the problem of landslide automatic recognition using supervised classification, and we also introduced the OPF classifier in this context. We employed two images acquired from Geoeye-MS satellite at March-2010 in the northwest (high steep areas) and north sides (pipeline area) covering the area of Duque de Caxias city, Rio de Janeiro State, Brazil. The landslide recognition rate has been assessed through a cross-validation with 10 runnings. In regard to the classifiers, we have used OPF against SVM with Radial Basis Function for kernel mapping and a Bayesian classifier. We can conclude that OPF, Bayes and SVM achieved high recognition rates, being OPF the fastest approach. © 2012 IEEE.

Avaliação do potencial genotóxico e mutagênico de águas e sedimentos do rio Guaecá, São Sebastião - SP, após impacto de oleoduto da região

Pós-graduação em Ciências Biológicas (Biologia Celular e Molecular) - IBRC