The effects of ethanol on PPARS in MCF-7 human breast cancer cells

Peroxisome proliferator-activated receptors (PPARs) are nuclear hormone receptors involved in various metabolic diseases. In the liver, PPARα is involved in alcohol metabolism and may lead to the development of alcoholic fatty liver and other alcohol mediated liver injuries. PPARβ modulation by ethanol induces abnormal myelin production by oligodendrocytes. PPARα and PPARβ are PPAR isoforms expressed in the human breast cell lines. Epidemiological studies show a positive correlation between alcohol intake and breast cancer risk, however, the molecular mechanisms involved are unclear. We hypothesized that ethanol would affect the expression and transactivation of human PPAR isoforms in estrogen receptor (ER) positive and ER negative breast cancer cells. Using real time RT-PCR we looked at the transcription of PPAR isoforms in the presence of increasing concentrations of ethanol and saw isoform and time dependent specific effects. Gene reporter assays enabled us to ascertain the effects of ethanol on ligand-mediated activation of human PPARα and PPARβ at concentrations equivalent to both moderate and chronic alcohol consumption. Ethanol differentially blocked the ligand-mediated activation of both PPARα and PPARβ. Since PPARα and PPARβ are involved in the differentiation and proliferation of breast cancer cells, PPARs may be a possible mechanism involved in the effect of ethanol in breast cancer.

A new breed of doctor? Lessons learned and changes made to library services for the MBBS program at the University of Queensland

November 2000 saw the graduation of the first cohort from the new MBBS Program at The University of Queensland. The fully integrated problem-based curriculum has provided many challenges and opportunities for The University of Queensland Library such as servicing an extensive remote student population, information technology issues and customer demand. In October 2000 all four years of the MBBS Program completed a survey on related issues such as the library's collection, web-based resources, information skills and evidence-based medicine. This paper focuses on the results of the survey, and the future directions of the library to ensure that the new doctors graduating from this program gain the essential attributes of life-long learning, information management and evidence-based principles.

UQ RoboRoos: Achieving power and agility in a small size robot

The UQ RoboRoos have been developed to participate in the RoboCup robot soccer small size league over several years. RoboCup 2001 saw a focus on the mechanical design of the RoboRoos, with the introduction of an omni-directional drive system and a high power kicker. The change in mechanical design had implications for the rest of the system particularly navigation and multi-robot planning. In addition, the overhead vision system was upgraded to improve reliability and robustness.

O IMPACTO DO CAPITAL PSICOLÓGICO E DO SUPORTE ORGANIZACIONAL SOBRE A SATISFAÇÃO NO TRABALHO

A necessidade de obter e manter níveis de excelência e de satisfação no trabalho cada vez mais altos leva as organizações a um crescente investimento em modelos de gestão orientados ao suporte dos seus colaboradores. Desse cenário emergem alguns questionamentos: o suporte organizacional impacta na satisfação dos colaboradores? Indivíduos com alto capital psicológico possuem um maior nível de satisfação no trabalho? Buscando responder a essas perguntas, o estudo teve como objetivo comparar e analisar o impacto individual e combinado dos construtos Percepção de Suporte Organizacional e Capital Psicológico na Satisfação no Trabalho. A pesquisa foi realizada com 304 profissionais de empresas públicas e privadas, com escolaridade mínima equivalente ao nível médio, de ambos os gêneros e idades variadas. O instrumento para coleta dos dados foi um questionário de autopreenchimento composto de três escalas: Satisfação no Trabalho EST, Percepção de Suporte Organizacional EPSO e Escala de Capital Psicológico ECP. Para atingir os objetivos propostos no modelo conceitual, foi utilizada a abordagem quantitativa. Os dados foram analisados por modelagem de equação estrutural pelo algoritmo dos mínimos quadrados parciais (PLS). Os resultados demonstraram que as variáveis Percepção de Suporte Organizacional e Capital Psicológico impactam positivamente a variável Satisfação no Trabalho, sendo que a Percepção de Suporte Organizacional exerce maior impacto na Satisfação do Trabalho do que Capital Psicológico.  

A RENÚNCIA SEXUAL PELO REINO: A IMPORTÂNCIA DA RENÚNCIA SEXUAL PARA A LIDERANÇA DA MULHER EM ATOS DE PAULO E TECLA

O propósito desta pesquisa é analisar a obra os Atos de Paulo e Tecla, focando na importância da renúncia sexual para a liderança da mulher. Tema este que se tornou fundamental para que as mulheres conseguissem conquistar seu espaço e poder diante da sociedade e assim conseguir fazer com que sua voz fosse ouvida, mesmo que de forma não tão considerável. Ao examinar os Apócrifos Atos de Paulo e Tecla, pôde-se observar suas principais características e composição, apresentando sua origem e interpretação pelas comunidades e pelos líderes do início do cristianismo. Buscou-se nesta pesquisa retratar e fundamentar o romance grego, apresentando suas similaridades com os Atos de Paulo e Tecla, e desta maneira destacar a influência do escrito grego sobre o texto apócrifo em sua forma de estruturação. Apresentamos as definições sobre a renúncia sexual, liderança e martírio sempre destacando os temas com a figura da mulher, abordando a questão do corpo e a sociedade e demonstrando como estes temas inspiraram os discursos no início do cristianismo. Referente aos escritos dos Atos Apócrifos, apresentamos a origem das comunidades de mulheres celibatárias, que influenciaram a escrita dos textos, relatando o motivo pelo qual os Atos foram escritos e como foram disseminados nas outras comunidades. Sobre o martírio, descrevemos suas origens e como os cristãos viam nessa ação uma aproximação com Deus, e como o martírio preparou a vida de Tecla para que ela se tornasse uma anunciante da palavra de Deus.

O CAMINHO DO CORDEIRO: REPRESENTAÇÃO E CONSTRUÇÃO DE IDENTIDADE EM APOCALIPSE 14.1-5

Ao analisar Apocalipse 14.1-5, esta pesquisa encontrou afirmações que parecem representar a identidade de João e sua audiência, bem como a forma como eles concebiam o mundo. Nos seus elementos litúrgicos, estas pessoas se viam como sacerdotes de Deus, membros do seu reino, participantes do seu culto celestial e com dignidade exa ltada como a dos anjos do céu. Alguns elementos identitários, entretanto, não são compartilhados plenamente entre João e suas comunidades. O autor de Apocalipse possui altas demandas ascéticas e sectárias que o afastam não apenas da sociedade mais ampla, mas de qualquer irmão que tenha uma posição divergente. Ele enxerga o mundo mergulhado num conflito entre o Dragão e o Cordeiro, conflito esse que será vencido com a participação de 144.000 guerreiros através da prática do martírio. Esta tradição da guerra santa insere no Apocalipse o potencial de isolar sectariamente a audiência da sociedade e de outros grupos religiosos.(AU)

DEBATE ATUAL SOBRE O GNOSTICISMO: UM OLHAR SOBRE O JESUS GNÓSTICO DE NAG HAMMADI

O encontro entre o cristianismo e a cultura grega foi um dos grandes eventos ocorridos na história da humanidade. Esse acontecimento possibilitou a expansão do cristianismo para além de sua terra natal já a partir do primeiro século d.C. Em curto espaço de tempo o cristianismo foi capaz de se estabelecer em diversas localidades do mundo antigo. Esse deslocamento propiciou sua divisão em várias vertentes, cada uma interpretando Jesus a sua maneira. A que se projetou para o norte do continente africano fomentou um tipo de cristianismo, ao que tudo indica, com traços gnósticos. Essa possibilidade veio à tona a partir de alguns escritos encontrados nessa região em meados do século XX e atribuídos a essa vertente cristã. Esta pesquisa tem como proposta analisar esse viés cristão com base nessas descobertas. Esse grupo, considerado minoritário, possuía algumas reivindicações frente ao cristianismo oficializado que os considerava herege. O alvo pesquisado foi à concepção de Jesus que os gnósticos sustentavam uma vez que se consideravam cristãos.

O SILÊNCIO DAS GERAIS: O nascimento tardio e a lenta consolidação dos jornais mineiros

Este trabalho analisou os fatores responsáveis pelo atraso no surgimento e no desenvolvimento dos jornais mineiros em suas principais fases: imprensa publicista, informativa e grande imprensa. As Minas, apesar de possuírem importância política e econômica, nos séculos XVIII e XIX, viram sua imprensa sempre assumir um papel secundário no país. Ela foi a sexta província a ter jornais, ficando atrás do Rio de Janeiro, Bahia, Pernambuco, Pará e Maranhão. Para entender o que deixou as Gerais nessa situação, foi necessário conhecer profundamente suas particularidades. A pesquisa demonstrou que a repressão a Inconfidência Mineira, os fluxos migratórios e as mudanças econômicas e sociais, que a província viveu no século XIX, foram responsáveis pelo atraso dos jornais mineiros. O próprio modo de ser do mineiro, a chamada mineiridade , também contribuiu para que isso ocorresse.(AU)

Does translation hinder integration?

2006 and 2007 saw renewed debates about rising costs of public sector translation and interpreting. One argument put forward was that the provision of such services for the immigrant population is deterring community cohesion in the UK. For example, the former education secretary Ruth Kelly raised the question of whether we are providing a ‘crutch’ by making translations available to immigrants, thus not giving them the incentive to learn English. The paper will address this question in the context of language policies and language ideologies in the UK. It will be argued that the debate about translation services has been instrumentalised for the discursive construction of identities.

The other on display: translation in the Ethnographic Museum

The ethnographic museum in the West has a long and troubling history. The display of 'exotic peoples' in travelling exhibitions began as early as the sixteenth century, but it was the mid and late nineteenth century that saw the great expansion of museums as sites to show artefacts collected - under anything but reputable circumstances - from what were considered the 'primitive', 'natural', or 'tribal' peoples of the world. Today the ethnographic museum is still a feature of large European cities, though faced with newly formulated dilemmas in the postcolonial world. For how can the material culture of a non-western people be collected and displayed in the West without its makers being translated into wordless and powerless objects of visual consumption? In national museums the processes of choosing, contextualizing and commentating exhibits help form national identity; in the ethnographic museum, similarly, they shape perceptions of the apparently distant Other. Like written ethnography, the museum is a 'translation of culture', with many of the associated problems traced by Talal Asad (1986). Like the written form, it has to represent the dialogic realities of cultural encounters in a fixed and intelligible form, to propose categories that define and order the material it has gathered. As the public face of academic ethnography, the museum interprets other cultures for the benefit of the general reader, and in that task museum practice, like all ethnography, operates within very specific historical and political parameters. How are museums in western Europe responding to the issues raised by critical ethnographers like James Clifford (1988), with their focus on the politics of representation? Is globalisation increasing the degree of accountability imposed on the ethnographic museum, or merely reinforcing older patterns? What opportunities and problems are raised by the use of more words - more 'translation' in the narrower sense - in ethnographic museums, and how do museums gain from introducing a reflexive and contextualizing concept of "thick translation" (Appiah 1993) into their work of interpretation?

Modulation of neuronal network activity in the primary motor cortex

In the present study I investigated the mechanisms of modulation of neuronal network activity in rat primary motor cortex using pharmacological manipulations employing the in vitro brain slice technique. Preparation of the brain slice in sucrose-based aCSF produced slices with low viability. Introducing the neuroprotectants N-acetyl-cysteine, taurine and aminoguanidine to the preparatory method saw viability of slices increase significantly. Co-application of low dose kainic acid and carbachol consistently generated beta oscillatory activity in M1. Analyses indicated that network activity in M1 relied on the involvement of GABAA receptors. Dose-response experiments performed in M1 showed that beta activity can be modulated by benzodiazepine site ligands. Low doses of positive allosteric modulators consistently desynchronised beta oscillatory activity, a mechanism that may be driven by a1-subunit containing GABAA receptors. Higher doses increased the power of beta oscillatory activity. Whole-cell recordings in M1 uncovered three interneuronal subtypes regularly encountered in M1; Fast-spiking, regular-spiking non-Pyramidal and low threshold spiking. With the paradoxical effects of positive allosteric modulators in mind, subsequent voltage-clamp recordings in FS cells revealed a constitutively active tonic inhibitory current that could be modulated by zolpidem in two different ways. Low dose zolpidem increased the tonic inhibitory current in FS cells, consistent with the desynchronisation of network oscillatory activity seen at this concentration. High dose zolpidem decreased the inhibitory tonic current seen in FS cells, coinciding with an increase in oscillatory power. These studies indicate a fundamental role for a tonic inhibitory current in the modulation of network activity. Furthermore, desynchronisation of beta activity in M1 decreased as viability of the in vitro brain slice increased, suggesting that the extent of desynchronisation is dependent upon the pathophysiological state of the network. This indicates that low dose zolpidem could be used as a therapeutic agent specifically for the desynchronisation of pathological oscillations in oscillopathies such as Parkinson’s disease.

New networked technologies and carers of people with dementia: an interview study

Dementia is one of the greatest contemporary health and social care challenges, and novel approaches to the care of its sufferers are needed. New information and communication technologies (ICT) have the potential to assist those caring for people with dementia, through access to networked information and support, tracking and surveillance. This article reports the views about such new technologies of 34 carers of people with dementia. We also held a group discussion with nine carers for respondent validation. The carers' actual use of new ICT was limited, although they thought a gradual increase in the use of networked technology in dementia care was inevitable but would bypass some carers who saw themselves as too old. Carers expressed a general enthusiasm for the benefits of ICT, but usually not for themselves, and they identified several key challenges including: establishing an appropriate balance between, on the one hand, privacy and autonomy and, on the other: maximising safety; establishing responsibility for and ownership of the equipment and who bears the costs; the possibility that technological help would mean a loss of valued personal contact; and the possibility that technology would substitute for existing services rather than be complementary. For carers and dementia sufferers to be supported, the expanding use of these technologies should be accompanied by intensive debate of the associated issues.

A study of the attitudes towards unethical selling amongst Chinese salespeople

The latter part of the twentieth century saw the Chinese economy moving towards a socialist market economy rather than a planned system. Despite growing interest in Chinese business ethics, little work has examined ethical issues concerning the Chinese sales force. This study draws from existing work on Chinese and Western business and sales ethics to develop hypotheses regarding the perceptions of unethical selling behaviour of modern Chinese salespeople. A survey of Chinese sales executives is conducted and statistically analysed. Results are compared with those reported in previous US-based research with regard to differences in perceptions of unethical selling behaviour. The results indicate that contemporary Chinese salespeople were more favourably disposed than expected towards unethical selling behaviour, and also more favourably disposed than previously studied US salespeople. Younger Chinese salespeople evaluated unethical behaviours more favourably than older ones. The results are discussed, along with implications for theory, practice and future work.