Caracterização de células de lugar no hipocampo e de suas relações com oscilações do potencial de campo local

The main inputs to the hippocampus arise from the entorhinal cortex (EC) and form a loop involving the dentate gyrus, CA3 and CA1 hippocampal subfields and then back to EC. Since the discovery that the hippocampus is involved in memory formation in the 50's, this region and its circuitry have been extensively studied. Beyond memory, the hippocampus has also been found to play an important role in spatial navigation. In rats and mice, place cells show a close relation between firing rate and the animal position in a restricted area of the environment, the so-called place field. The firing of place cells peaks at the center of the place field and decreases when the animal moves away from it, suggesting the existence of a rate code for space. Nevertheless, many have described the emergence of hippocampal network oscillations of multiple frequencies depending on behavioral state, which are believed to be important for temporal coding. In particular, theta oscillations (5-12 Hz) exhibit a spatio-temporal relation with place cells known as phase precession, in which place cells consistently change the theta phase of spiking as the animal traverses the place field. Moreover, current theories state that CA1, the main output stream of the hippocampus, would interplay inputs from EC and CA3 through network oscillations of different frequencies, namely high gamma (60-100 Hz; HG) and low gamma (30-50 Hz; LG), respectively, which tend to be nested in different phases of the theta cycle. In the present dissertation we use a freely available online dataset to make extensive computational analyses aimed at reproducing classical and recent results about the activity of place cells in the hippocampus of freely moving rats. In particular, we revisit the debate of whether phase precession is due to changes in firing frequency or space alone, and conclude that the phenomenon cannot be explained by either factor independently but by their joint influence. We also perform novel analyses investigating further characteristics of place cells in relation to network oscillations. We show that the strength of theta modulation of spikes only marginally affects the spatial information content of place cells, while the mean spiking theta phase has no influence on spatial information. Further analyses reveal that place cells are also modulated by theta when they fire outside the place field. Moreover, we find that the firing of place cells within the theta cycle is modulated by HG and LG amplitude in both CA1 and EC, matching cross-frequency coupling results found at the local field potential level. Additionally, the phase-amplitude coupling in CA1 associated with spikes inside the place field is characterized by amplitude modulation in the 40-80 Hz range. We conclude that place cell firing is embedded in large network states reflected in local field potential oscillations and suggest that their activity might be seen as a dynamic state rather than a fixed property of the cell.

Efeito agudo do exercício físico associado a praxias ideomotoras sobre as concentrações séricas de cortisol e memória declarativa em indivíduos com idade entre 51 e 74 anos

Introduction: Several modifications are identified as aging, causing more or less limitation imposed by over the years. Among these, one can highlight the different degrees of cognitive decline, particularly memory that can involve the daily activities and the subject functionality. Studies have shown an association between levels of serum cortisol and stress imposed by the exercise on this. However, there are few studies that references the performance on cognitive aspects of declarative memory and cortisol on the exercise in the water with automatic and práxicos movements and moderate. Objective: Check the effect based on the acute physical exercise and práxicos automatic movements on the performance of visual declarative memory and in serum cortisol in subjects aged between 51 and 74 years. Materials and Methods: It builds a survey characterized as cross with a first sample of 32 physically active subjects aged between 51 and 74 years, divided into two exercise groups (March of Automatic Group - MAG and the March of Praxis Group - MPG). We used a probabilistic and random sampling for sample selection. Used the MMSE (Mini Mental State Examination) to check the general cognitive status, visual acuity test - optotypes chart "E" Rasquin and was even used the declarative visual memory test proposed by Nitrini and collaborators (1994), applying before motor stimulation and immediately after, and the day of blood collection with 2 ml for analysis of cortisol hormone. The normality and homogeneity were verified from the Shapiro-Wilk and Levene tests. Thus we adopted a descriptive statistics to characterize the sample. The Split-Plot ANOVA was used along with the paired t-test to verify the identified differences. We adopted a significance level of p <0.05. Results: It was observed that the groups (MAG and MPG) and the anthropometric variables, perceived exertion, education, cognitive assessment and visual acuity showed no significant differences (p > 0.05), showing that the groups are homogeneous, with variables and similar means. After the stimulation session, lasting 30 min, it was observed that the amount of hits for Δ of declarative memory questionnaire visual images increased, presenting significant for both groups (MAG, p < 0.001; MPG, p = 0.042). The same was observed for cortisol concentration with a reduction in the levels immediately after the stimulus (MAG and MPG, p < 0.001). Conclusion: The results showed that the exercises proposed in its acute effect provide significantly memories of gains and also showed a reduction in cortisol levels.

Terra de charneca erma e de saudade: a construção simbólica do Alentejo português na obra de Florbela Espanca (1916-1930)

In this work, we investigate the symbolic construction of a particular spatiality, starting from the theoretical assumption that spaces are subjective constructions guided by different cultures, feelings and rationales, but mostly spaces are human constructs driven by social relations, as a result of the material investment and symbol that reflects the needs of a particular society at a given time of historical development. Accordingly, we analyze the construction and symbolic imagery of the central region of Portugal, the Alentejo, from the literary production (1916 – 1930) the English poet Florbela Espanca D'Alma Conception Espanca. Thus, we propose to analyze the florbelian work not only from its internal relations, but also external, emphasizing the link between history, space and literature. Thus, we propose to inquire about the symbolic dimension – the meanings of images and representations – which prompted one of the most controversial Portuguese poets of the early twentieth to look into the poetic construction of space Alentejo century, questioning not only the senses brokered by speech literary Florbela Espanca to invent your Alentejo, adorned with memories, pain and longing, but investigate how the socio-cultural environment influenced your work, in your life and ways to feel and live the Alentejo. To better understand how the poet means the Alentejo spatiality, throughout this work we question three categories of space in the work of Florbela Espanca: the region, the countryside and the landscape of the Alentejo. Thus, this research falls within the field of cultural history in the medical we will work with the entire literary output Florbela Espanca, letters, diaries, photos and biographical and literary criticism, by establishing the time frame of 1916 – beginning of intellectual activity Florbela Spank – the 1930 – publication of Blossom Heath (posthumous) and the suicide of the poet. Therefore, a constant symbolic exercise of words crossed by more subjective feelings of the subject, all the time our work will be guided by the question of what would be the Alentejo for the poet, who senses and meanings across this spatiality that marked so sovereignly happiest memories and sad life Florbela Espanca.

Crises do cotidiano em Machado de Assis e Luigi Pirandello: um estudo comparado

This thesis proposes to identify possible similarities and differences between the novels Memórias Póstumas de Brás Cubas (1981), by the Brazilian writer Machado de Assis, and Uno, Nessuno and Centomila (1926), by the Italian writer Luigi Pirandello. The two authors have prominent respective places in Brazilian and Italian literature, and both observed and experienced remarkable changes in their societies Brazil's imperial period, and Italy’s post-risorgimentale period. We will verify how each author composes a piece o literary art in which it is possible to achieve the authors’social and moral conscience. We will also attempt to achieve thefeelings of restlessness, anxiety, fear, doubt, interest, vanity, ambition: in summary, the desire of the characters to exist, which represents man at the end of the nineteenth century and commencement of thetwentieth century. The characters’ features point to fragmented identity, in search of a place in the world, even if he needs to renounce his essence and to adopt a corresponding appearance to all images that society assigns to him in order to achieve such a place. The protagonists, Brás Cubas and Vitangelo Moscarda, will lead us along the paths of consciousness of each, which demarcate the border between essence and appearance. Critics such as Roberto Schwarz, Alfredo Bosi, Leone Castris, among others, will give us the theoretical support necessary for a comparative study between the two authors, whom, like few others, knew how to express, through their characters, the difficult relationship of man with himself and with the universe at large.

Olimpíada de Língua Portuguesa: ressignificação de práticas de leitura e escrita

The search for new meanings in the basic education teaching-learning process has caused the development of public policies for mother language teaching, such as the Portuguese Language Olympics (OLP). To contribute to this search, this intervention project has as object of study reading and writing practices developed in the OLP through the educational model arising from literacy projects (TINOCO, 2008). In working towards, the general aim of reframing reading and writing practices through the PLO, developed from the teaching model that comes of literacy projects, we established three specific objectives: a) reflect on a national writing contest; b) to realign conceptual and methodological the Portuguese classes of the 7th grade school due to the developed project; c) to improve the reading and writing practices of the students in 7th grade of school where we operate. Therefore, we base ourselves in the history of Portuguese teaching in Brazil (SOARES, 2002; GERALDI, 2008), the dialogical conception of language (BAKHTIN, VOLOCHÍNOV [1929] 2009; SOARES, 1998; FARACO, 2009) in Literacy Studies (KLEIMAN, 2001, 2005, 2006; TINOCO, 2008; OLIVEIRA; TINOCO; SANTOS, 2011; STREET, 2014), the learning community concept (AFONSO, 2001), in studies of retextualization (OLIVEIRA, 2005; MARCUSCHI, 2010), gender discursive literary memories (CLARA; ALTENFELDER; ALMEIDA, 20--), in written evidence (POSSENTI, 2002) and Textual Linguistics (MARCUSCHI, 2008; ANTUNES, 2009; KOCH, 2011; SILVA [et. al.], 2013). Methodologically, this qualitative research (LÜDKE; ANDRÉ, 1986; ANDRÉ, 2005) is anchored in Applied Linguistics (MOITA LOPES, 1996). This research was supporting by students in the 7th grade, teachers, management team and parents, as well as people outside of school community. The instruments used for the generation of data were semi-structured interview, students‟ texts, audio recordings and video, photos, OLP material (teacher's book, a collection of texts and CD-ROM). The data generated allowed us to establish the following categories of analysis in relation to the texts produced: authorship, in formativeness, discursive progression, compositional structure, content, style, and language aspects. In addition, throughout the project, the collaborators have produced texts of various genres: oral interview and written request letter, legal, literary memories, oral and experience report. Also experienced a local award and participated in a national competition. They produced a video and a book with stories and student authorship of illustrations. The results achieved show that the literacy project developed also allowed macro changes: reading and writing practices, once considered strictly school studied, they were transformed into broader social practices, through which various literacy agents were able to collaboratively act. In short, they experienced writing practices that go beyond the classroom and the teacher-student relationship.

Irish Coffee: efeitos de álcool e cafeína no comportamento e aprendizagem do peixe paulistinha

Substâncias psicoestimulantes vêm sendo utilizadas de forma indiscriminada há muitos anos, e pouco se sabe os efeitos que elas causam a curto e longo prazo no comportamento geral, na aprendizagem e na memória. Essas substâncias são bastante usadas por jovens e adultos e elas possuem efeitos diferentes. Essas substâncias são dose dependente, caso consumidas em baixa quantidade agem como estimulante, aumentando a atividade locomotora, caso consumidas em alta quantidade, causam efeito depressor, diminuindo a atividade locomotora e/ou causando ansiedade. Poucos estudos vêm investigando os efeitos dessas substâncias na atividade locomotora, aprendizagem e memória e grande parte desses estudos são realizados em roedores. Peixe paulistinha é um modelo animal promissor para estudos comportamentais, cognitivos, ontogenéticos, dentre outros. Nossos objetivos foram determinar os efeitos do álcool, cafeína e de seu uso combinado com álcool, na atividade locomotora desses animais, usando para isso doses crônicas durante 27 dias e doses agudas durante um dia. Visto que pouco se sabe sobre os efeitos dessa exposição prolongada. Também investigamos os efeitos das substâncias em teste de reconhecimento de objetos, que se baseia na memória de único evento. Essas memórias são mais vulneráveis que memórias baseadas em várias repetições de eventos. Sendo assim, um teste adequado para utilizar com uso de substâncias psicoativas. Observamos que o uso crônico de cafeína provoca alteração na atividade locomotora dos animais, do mesmo modo, abstinência de álcool combinada com cafeína em dose aguda (dose média) provoca aumento de atividade locomotora. Quando submetidos a testes de memória, os animais exposto a doses altas agudas de álcool e em abstinência dessa droga têm prejuízo na formação e/ou resgate da memória. No entanto, tratamento com cafeína não prejudica a formação de memória. Animais expostos a tratamento com dose crônica moderada de álcool e dose aguda moderada de cafeína tem melhor desempenho na tarefa, indicando que dose aguda moderada de cafeína pode evitar os efeitos deletérios ocasionados pela abstinência do álcool. Em termos do comportamento geral, doses agudas de cafeína aumentam a locomoção, enquanto doses elevadas e a abstinência de cafeína induzem a comportamentos tipo-ansioso. A combinação álcool crônico e cafeína aguda induzem a alto comportamento tipo-ansiedade, enquanto a combinação cafeína crônica e álcool agudo diminuem tanto a locomoção quanto a ansiedade.

Irish Coffee: efeitos de álcool e cafeína no comportamento e aprendizagem do peixe paulistinha

Substâncias psicoestimulantes vêm sendo utilizadas de forma indiscriminada há muitos anos, e pouco se sabe os efeitos que elas causam a curto e longo prazo no comportamento geral, na aprendizagem e na memória. Essas substâncias são bastante usadas por jovens e adultos e elas possuem efeitos diferentes. Essas substâncias são dose dependente, caso consumidas em baixa quantidade agem como estimulante, aumentando a atividade locomotora, caso consumidas em alta quantidade, causam efeito depressor, diminuindo a atividade locomotora e/ou causando ansiedade. Poucos estudos vêm investigando os efeitos dessas substâncias na atividade locomotora, aprendizagem e memória e grande parte desses estudos são realizados em roedores. Peixe paulistinha é um modelo animal promissor para estudos comportamentais, cognitivos, ontogenéticos, dentre outros. Nossos objetivos foram determinar os efeitos do álcool, cafeína e de seu uso combinado com álcool, na atividade locomotora desses animais, usando para isso doses crônicas durante 27 dias e doses agudas durante um dia. Visto que pouco se sabe sobre os efeitos dessa exposição prolongada. Também investigamos os efeitos das substâncias em teste de reconhecimento de objetos, que se baseia na memória de único evento. Essas memórias são mais vulneráveis que memórias baseadas em várias repetições de eventos. Sendo assim, um teste adequado para utilizar com uso de substâncias psicoativas. Observamos que o uso crônico de cafeína provoca alteração na atividade locomotora dos animais, do mesmo modo, abstinência de álcool combinada com cafeína em dose aguda (dose média) provoca aumento de atividade locomotora. Quando submetidos a testes de memória, os animais exposto a doses altas agudas de álcool e em abstinência dessa droga têm prejuízo na formação e/ou resgate da memória. No entanto, tratamento com cafeína não prejudica a formação de memória. Animais expostos a tratamento com dose crônica moderada de álcool e dose aguda moderada de cafeína tem melhor desempenho na tarefa, indicando que dose aguda moderada de cafeína pode evitar os efeitos deletérios ocasionados pela abstinência do álcool. Em termos do comportamento geral, doses agudas de cafeína aumentam a locomoção, enquanto doses elevadas e a abstinência de cafeína induzem a comportamentos tipo-ansioso. A combinação álcool crônico e cafeína aguda induzem a alto comportamento tipo-ansiedade, enquanto a combinação cafeína crônica e álcool agudo diminuem tanto a locomoção quanto a ansiedade.

Um brinde à cachaça: o patrimônio históricocultural e seus usos turísticos nos alambiques do Rio Grande do Norte

The study proposes to present a discussion about the value of cachaça as a cultural heritage and its relation with the touristic activities in the State of Rio Grande do Norte. Based on a discussion originated from historic studies related to the touristic sector, especially when it comes to gastronomy, cachaça is defined not only as an important instrument to the construction of identities, but, also, as an element able to weave social, political and economic relations. Therefore, a touristic product that can provide new destinations. The research was made through five alembics located on the East and Seridó regions of Rio Grande do Norte. To define the focus, it is necessary to refine this selection, especially when dealing with historical facts and memories from these establishment owners. Thus, it becomes necessary to consider the alembic trajectory, periods of larger productions and insertion in the touristic market. Through documental data collection and observations made in field tours, the research has a qualitative approach with descriptive and exploratory goal, methodology that allows us to approach issues around articulations among heritage, identity and tourism. "It was concluded that the state of Rio Grande do Norte has many tourist routes where handmade cachaça produced in the State can be inserted in any of these routes due to its potential searched in the research, as well as, it is possible to work in other perspective when it comes to ways of protecting this patrimony and strategies of incentive of the activity for the development and appreciation of local economy.

"Ombro a ombro com os mais fracos": a inserção de João Café Filho nos espaços do trabalhador na cidade do Natal 1922 - 1937

The purpose of this thesis is to analyze how João Café Filho constituted a discourse of advocate of the labor movement and workers in different sociability spaces. It is intended to understand, on one hand, how political relations were established between different categories of workers and the ‘middle classes’ and, on the other hand, how places were instituted to house the meeting of these relations. It a ims to understand the insertion of Café Filho in union activities in the urban world. It demonstrates specificities of the political culture in Natal emphasizing the dispute between a city politically ruled by a still reigning rural paternalistic mentality and the rise of a new way to experience the urban conflicts which appeared. Temporally, the work is delimited between 1922 (proclaimed by Café Filho himself as the initial period of his political action) and 1937 (when he broke up with Vargas and went into exile in Argentina). The research was constituted by three main document types: several published newspapers between the decades of 1920 and 1930 in the cities of Natal, Recife, São Paulo, Porto Alegre and Rio de Janeiro; the autobiographical memoirs written by Café Filho himself and memoirs of other people who lived in his time. The main pillars that have supported the work were: the concepts of society and individuals (ELIAS, 1994; 1995), political cultures (BERSTEIN, 1998) and theater of the memory (GOMES, 2004); the sociability spaces category (CERTEAU, 1994; MALATIAN, 2001; RIOX, 1996); the biography notion (DOSSE, 2009; LORIGA, 2011). We demonstrated that Café Filho acted in some sociability spaces as: the Jornal do Norte, the Federação Regional do Trabalho and the Partido Democrático Nacional. In such spaces, Café Filho, gradually, become an important leader of workers and, at the same time, linked to national entities led to the opposition that fight against the power established in the Brazilian First Republic. In Café Filho’s interpretation, workers were individuals who needed to fight against the political structures prevailing at that time because the poor living conditions and the low representativeness of this group were caused by the way the political system in the First Republic was structured. After the 1930 Movement, the 3 de Outubro Club, the Jornal and the Labor Federation of Natal were constituted in spaces where the cafeista critical discourse about the government was changed: workers should follow the official syndicalism and defend the 1930 Movement which put Vargas in the presidency of the Republic.

Um olhar sobre “Água Suja” e o “Moleque”: tradição, fé, cultura e teatralidade

This thesis addresses two spectacles: “Moleque tão Grande Otelo” and “Água Suja”, in which both have had my participation as producer and actress. The spectacle “Moleque tão Grande Otelo” reveals the racial-ethnic questions that surrounds the play and permeates the artist’s life whose name is the same. The play deals how the actor Grande Otelo life events’ had implied in his art, the facts that marked his career and they are shown at the spectacle, the reason why to articulate: art, life, fantasy and reality. Aiming to unveil the actor, I refer it to my history as an actress and the roots’ similarity to Sebastião Bernardes de Souza Prata, who became Grande Otelo to the world. In the staging of “Água Suja”, we also expose familial and religious memories and experiences of a people that overflow faith in a real spectacle, which takes place every year, in August, in our region, more specifically in Romaria – MG. While creating “Água Suja”, we experienced the act of keeping a promise and believing in the saint’s miracle, thus this experience is told in the spectacle and investigated in this study.

Que coisa é essa, Yôyô?: cor e raça na imprensa ilustrada da Bahia (1897-1904)

From techniques such as lithography and woodcut, it was possible to create and reproduce daily images in the newspapers of the Empire and the Republic of Brazil. The purpose of this study is to make a historiographic report, derived from a multidisciplinary theoretical analysis to which several printed visual documents were selected from the newspaper A Coisa from Salvador, in Bahia. The weekly news, edited in the capital and distributed also in the countryside of Bahia by the end of 1897 and the beginning of 1904 is rich for its illustrations and the satirical, humorous and critical content, signed by its editors. The images in A Coisa are appealing for their content filled with tensions inherent to the time of the First Republic in Brazil, such as issues regarding ones skin color, phenotypes, race, gender, the value and the social ranking of the black population. The paper, in its gathering of texts and images, is the main basis of this research corpus, in which a dialogue with other papers from other places and times is proposed so that it becomes evident the historical process that marks the ideal of nation and the construction of a body and an identity for the people of African Descent in Brazil. The observation and analysis of the selected images from the newspaper allow the identification of its way of production, the orientation of a reality in function of its target consumers, their authorship and the objectives to which it was created. Therefore, this work aims to critically analyze the representations given to the black body and skin, in order to problematize the memories of these bodies and their sociocultural meanings and, thus, question, through a methodology aimed to the description and analysis of images united to texts, these bodies visual representations possible contribution to the formation of an idea of black people unified identity, and their social alterity in deference to the memories given to the white society in the historical and social context of that time.

GESTÃO DA CULTURA NA UNIVERSIDADE: O NÚCLEO DE ARTE E CULTURA DA UNIVERSIDADE METODISTA DE SÃO PAULO

Essa dissertação resulta de um processo de reflexão autobiográfica, com base no resgate de memórias e vivências do percurso de vida-trabalho da pesquisadora, a partir da experiência na gestão do Núcleo de Arte e Cultura (NAC) da Universidade Metodista de São Paulo (UMESP), desde a sua criação, em 2005. Compreender o processo de criação, constituição e inserção do NAC na UMESP, como também o exercício da gestão cultural nesse espaço universitário, foram objetivos dessa pesquisa, que destaca o diálogo entre Educação e Cultura, mediado pela Arte, a partir da inserção de ações artístico-culturais focadas no respeito às diferenças e na valorização da diversidade humana e cultural. Qual o papel da ação cultural dentro de uma universidade? Como essas ações podem somar com o processo de formação educacional? Que desafios envolveram a construção e constituição do Núcleo de Arte e Cultura da UMESP? Essas são questões geradoras do desenvolvimento desse trabalho, no qual é utilizada a abordagem (auto)biográfica como metodologia de pesquisa. Na narrativa propiciada por essa abordagem, a pesquisadora procurou revisitar e ressignificar, numa perspectiva de produção de conhecimento, memórias e histórias que contribuíram para a construção da sua própria identidade e que alavancaram sua inserção na gestão da Cultura na UMESP, legitimando e firmando a atuação do Núcleo de Arte e Cultura, ao longo dos seus 10 anos de existência. Ao refletir sobre essa práxis, evidenciou-se que trabalhar com as subjetividades é o grande desafio da gestão da Cultura, como também a construção de processos colaborativos, na perspectiva do “fazer com” e não do “fazer para” é o caminho para o fortalecimento e enraizamento das ações.

Os Parques Infantis de Mario de Andrade

Os Parques Infantis de Mario de Andrade pretende mostrar o cenário físico, cultural e social da cidade de Sao Paulo entre 1930 - 1940 e a realizaçao desta inovadora instituiçao de atendimento à infância e a juventude das classes operárias. Trata-se de uma pesquisa qualitativa, histórica com revisao de literatura em Língua Portuguesa. Dentre muitos serviços à populaçao, Mário de Andrade, Secretário da Cultura do Município de Sao Paulo, e escritor de Pauliceia Desvairada, idealizou e implementou os Parques Infantis para crianças e jovens paulistanos. Os Parques Infantis educavam seusfrequentadores de forma plena; os serviços ali prestados incluindo - o médico odontológicos - representavam mais que um atendimento aos abandonados aprópria sorte. A elaboraçao de jornal, atividades de leitura, as cívicas, motoras e lúdicas eram e supervisionadas especialistas. Dos Parques Infantis idealizados ficou memórias esparsas, alguns registros iconográficos e uma única (hoje) escola que pude conhecer