Iowa Department of Elder Affairs Legislative Update with Attachments, February 17, 2006, Vol. 2, no. 6

Iowa Department of Elder Affairs provides a brief summation of a Departmental program or other important Departmental information in each Legislative Update. The Legislative Update is produced for informational and educational purposes only.

Iowa Lottery Action, April 17-30, 2006, vol. 13, no. 8

Iowa Lottery newsletter for Lottery retailers.

Executive Order Number Twenty Five, November 17, 1986

Amend Executive Order #22 to Provide a procedure for allocation the total principal amount of bonds which can be issued by political subdivisions of the State during any calender year.

Executive Order Number Thirty Eight, March 17, 1989

In complaiance with the Drug Free Workplace Act of 1988, State of iowa is obliged to provide a drug and alcohol free environment including disciplinary action.

A novel interferon-gamma regulated human melanoma-associated antigen, gp33-38, defined by monoclonal antibody Me14-D12. II. Molecular cloning of a genomic probe.

The human Me14-D12 antigen is a cell surface glycoprotein regulated by interferon-gamma (IFN-gamma) on tumor cell lines of neuroectodermal origin. It consists of two non-convalently linked subunits with apparent mol. wt sizes of 33,000 and 38,000. Here we describe the molecular cloning of a genomic probe for the Me14-D12 gene using the gene transfer approach. Mouse Ltk- cells were stably cotransfected with human genomic DNA and the Herpes Simplex virus thymidine kinase (TK) gene. Primary and secondary transfectants expressing the Me14-D12 antigen were isolated after selection in HAT medium by repeated sorting on a fluorescence activated cell sorter (FACS). A recombinant phage harboring a 14.3 kb insert of human DNA was isolated from a genomic library made from a positive secondary transfectant cell line. A specific probe derived from the phage DNA insert allowed the identification of two mRNAs of 3.5 kb and 2.2 kb in primary and secondary L cell transfectants, as well as in human melanoma cell lines expressing the Me14-D12 antigen. The regulation of Me14-D12 antigen by INF-gamma was retained in the L cell transfectants and could be detected both at the level of protein and mRNA expression.

In vivo localization of a bispecific antibody which targets human T lymphocytes to lyse human colon cancer cells.

A bispecific MAb was derived from the fusion of a hybridoma producing MAb CD3 with a hybridoma producing MAb L-DI (which is directed against a 41-kDa glycoprotein expressed in most gastro-intestinal and pancreatic carcinomas). Bispecific antibody molecules were isolated from parental antibody molecules by the use of hydroxylapatite-HPLC and shown to target human cytolytic T lymphocytes, irrespective of their original specificity, to specifically lyse human colon carcinoma cells. Localization studies in vivo using nude mice bearing human colon carcinoma xenografts showed significant accumulation of the HPLC-purified 125I-labelled bispecific antibodies into the tumor compared to 131I-labelled control CD3 antibody.

Kangasniemi 17.1.1940

Kirje

Hina 17.8.1943

Kirje

Lottery Action Newsletter, September 9-24, 2006, Vol. 13, no. 17

Iowa Lottery Retailer Newsletter

ABD e-News, November 17, 2006

Weekly Newsletter

Motor Vehicle Accident Fatalities for the Week Ending, November 17, 2006

Record of the Fatalities for Motor Vehicle Accidents in Iowa per week.