Abscisic acid regulation of plant defence responses during pathogen attack

The plant hormone, abscisic acid (ABA), has previously been shown to have an impact on the resistance or susceptibility of plants to pathogens. In this thesis, it was shown that ABA had a regulatory effect on an extensive array of plant defence responses in three different plant and pathogen interaction combinations as well as following the application of an abiotic elicitor. In unique studies using ABA deficient mutants of Arabidopsis, exogenous ABA addition or ABA biosynthesis inhibitor application and simulated drought stress, ABA was shown to have a profound effect on the outcome of interactions between plants and pathogens of differing lifestyles and from different kingdoms. The systems used included a model plant and an important agricultural species: Arabidopsis thaliana (Arabidopsis) and Peronospora parasitica (a biotrophic Oomycete pathogen), Arabidopsis and Pseudomonas syringae pathovar tomato (a biotrophic bacterial pathogen) and an unrelated plant species, soybean (Glycine max) and Phytophthora sojae (a hemibiotrophic Oomycete pathogen), Generally, a higher than basal endogenous ABA concentration within plant tissues at the time of avirulent pathogen inoculation, caused an interaction shift towards what phenotypically resembled susceptibility. Conversely, a lower than basal endogenous ABA concentration in plants inoculated with a virulent pathogen caused a shift towards resistance. An extensive suppressive effect of ABA on defence responses was revealed by a range of techniques that included histochemical, biochemical and molecular approaches. A universal effect of ABA on suppression or induction of the phenylpropanoid pathway via regulation of the key entry point gene, phenylalanine ammonia-lyase (PAL), when stimulated by biotic or abiotic elicitors was shown. ABA also influenced a wide variety of other defence-related components such as: the development of a hypersensitive response (HR), the accumulation of the reactive oxyden species, hydrogen peroxide and the cell wall strengthening compounds lignin and callose, accumulation of SA and the phytoalexin, glyceollin and the transcription of the SA-dependent pathogenesis- related gene (PR-1). The near genome-wide microarray gene expression analysis of an ABA induced susceptible interaction also revealed an yet unprecedented insight into the great diversity of defence responses that were influenced by ABA that included: disease resistance like proteins, antimicrobial proteins as well as phenylpropanoid and tryptophan pathway enzymes. Subtle differences were found in the number and type of defence responses that were regulated by ABA in each type of plant and pathogen interaction that was studied. This thesis has clearly identified in plant/pathogen interactions previously unknown and important roles for ABA in the regulation of many defence responses.

Bearing the risk : learning to be drier mid-river

This paper investigates learning related to the phenomena of drying over the past decade in the southern Murray-Darling Basin in Australia, as perceived in a mid-river site within the western Riverina of New South Wales, Australia. The insights from audio-recorded interviews, with a wide range of adults across the water-dependent community, mostly relate to the catchment of the  Murrumbidgee River in the Shire of Hay. Our overarching theme is about how  people are learning about, understanding and bearing the risks, of what is widely regarded as a prolonged drought. For some, the learning is about how to cope with less water in the Basin, and particularly from the river, as predicted in the climate change literature. Our narrative-based, empirical research registers the felt experience of those located, in situ, as a severe ‘irrigation drought’ extends into 2009. The paper dramatises the many obstacles to learning how to think and act differently, in difficult and rapidly changing ecosocial circumstances.

Train the trainer model : implications for health professionals and farm family health in Australia

Australia is a large country with 60% of land used for agricultural production. Its interior is sparsely populated, with higher morbidity and mortality recorded in rural areas, particularly farmers, farm families, and agricultural workers. Rural health professionals in addressing health education gaps of farming groups have reported using behavioralist approaches. These approaches in isolation have been criticized as disempowering for participants who are identified as passive learners or 'empty vessels.' A major challenge in rural health practice is to develop more inclusive and innovative models in building improved health outcomes. The Sustainable Farm Families Train the Trainer (SFFTTT) model is a 5-day program developed by Western District Health Service designed to enhance practice among health professionals working with farm families in Australia. This innovative model of addressing farmer health asks health professionals to understand the context of the farm family and encourages them to value the experience and existing knowledge of the farmer, the family and the farm business. The SFFTTT program has engaged with health agencies, community, government, and industry groups across Australia and over 120 rural nurses have been trained since 2005. These trainers have successfully delivered programs to 1000 farm families, with high participant completion, positive evaluation, and improved health indicators. Rural professionals report changes in how they approach health education, clinical practice, and promotion with farm families and agricultural industries. This paper highlights the success of SFFTTT as an effective tool in enhancing primary health practice in rural and remote settings. The program is benefiting not only drought ravaged farmers but assisting rural nurses, health agencies, and health boards to engage with farm families at a level not identified previously. Furthermore, nurses and health professionals are now embracing a more 'farmer-centered model of care.'

Fresh water: new perspectives on water in Australia

Is water a resource or is it the source? Is it something to be consumed or does it have a life of its own? Recent histories of environmental misunderstanding and exploitation shadow our current regime of water management and use. While governments grapple with how to respond to widespread drought, the situation worsens. There is something amiss in current approaches to water. This timely collection of essays addresses the critical and contentious issue of water in Australia today and suggests a need to radically rethink our relationship with this fundamental substance. Contributors from a range of fields, from anthropology to visual arts, discuss the various ways in which we are caught up with water, and challenge us to take up the cultural transformations that underpin a sustainable ecological future

Small-scale irrigation, pastoral livelihood diversification and poverty alleviation in Kenya

Pastoral traditional livelihood systems are under threat from drought and human predation. Those who diversified into irrigation farming are less vulnerable and able to re-invest in the pastoral economy and alternative livelihood activities. Small-scale irrigation has contributed towards economic growth and poverty alleviation in the target area. These changes may however not be sustainable.

Water savings or water efficiency? Water-use attitudes and behaviour in rural and regional areas

Drought conditions in southwest Victoria, as in other regions of Australia and around the world, have caused the need to reduce water consumption to ensure security of supply into the future. To develop effective water-saving behaviour change strategies, an understanding of people's attitudes to the behaviour, including barriers stopping them from adopting the behaviour, is required. Thus, this paper explores the water-use behaviours and attitudes of rural and regional urban water users in southwest Victoria. A conceptual model of the factors impacting on water use of these users, including drivers and barriers to water saving, is developed. The factors that appear to impact on water-use behaviour not previously identified included the source of water supply (groundwater versus surface water), previous experience with water shortages and trust in the water authority and government. Also, a difference in the drivers for water saving was found, with farmers wanting to be 'water efficient' to keep their business viable and productive, while hobby farmers and residential users were 'saving water' for more altruistic reasons. These findings have implications for development of demand management strategies in this, and other, rural and regional areas. However, the conceptual model has to be tested to determine if it truly reflects factors influencing water-saving behaviour in rural and regional areas.

Understanding drivers and barriers : the key to water use behaviour change

In southwest Victoria, like many other regions in Australia, drought, climate change and population growth have exposed gaps in water supply. To develop effective demand management strategies for rural and regional areas, this paper investigates the drivers and barriers to water saving in southwest Victoria. Although the majority of people felt water saving was important, the drivers for water saving differed between different groups. Residential users were saving water for altruistic reasons, while for farmers the drivers were farm viability and productivity. Although the barriers differed between property types, common barriers included lack of understanding of the impact their water use has on supplies, lack of knowledge, the pricing system and distrust of the water authority. The findings provide information for effective demand management strategies for the region.

Climate change impacts and adaptation in Bangladesh : an agent-based approach

Bangladesh exemplifies the complex challenges facing densely populated coastal regions. The
pressures on the country are immense: around 145 million people live within an area of just 145,000 sq-km at
the confluence of three major river systems: the Ganges, the Brahmaputra and the Meghna. While progress
has been made, poverty remains widespread, with around 39% of children under five malnourished. Most of
its land-mass lies below 10m above sea level with considerable areas at sea level, leading to frequent and
prolonged flooding during the monsoons. Sea level rise is leading to more flooding as storm surges rise off
higher sea levels, pushing further inland. Higher sea levels also result in salt-water intrusion into freshwater
coastal aquifers and estuaries, contaminating drinking water and farmland. Warmer ocean waters are also
expected to lead to an increase in the intensity of tropical storms.
Bangladesh depends on the South Asian summer monsoon for most of its rainfall which is expected to
increase, leading to more flooding. Climate scientists are also concerned about the stability of monsoon and
the potential for it to undergo a nonlinear phase shift to a drier regime. Bangladesh faces an additional
hydrological challenge in that the Ganges and Brahmaputra rivers both rise in the Himalaya-Tibetan Plateau
region, where glaciers are melting rapidly. The Intergovernmental Panel on Climate Change (IPCC)
concluded that rapid melting is expected to increase river flows until around the late-2030s, by which time
the glaciers are expected to have shrunk from their 1995 extent of 500,000 sq-km to an expected 100,000 sqkm.
After the 2030s, river flows could drop dramatically, turning the great glacier-fed rivers of Asia into
seasonal monsoon-fed rivers. The IPCC concluded that as a result, water shortages in Asia could affect more
than a billion people by the 2050s. Over the same period, crop yields are expected to decline by up to 30% in
South Asia due to a combination of drought and crop heat stress. Bangladesh is therefore likely to face
substantial challenges in the coming decades.
In order to adequately understand the complex, dynamic, spatial and nonlinear challenges facing Bangladesh,
an integrated model of the system is required. An agent-based model (ABM) permits the dynamic
interactions of the economic, social, political, geographic, environmental and epidemiological dimensions of
climate change impacts and adaptation policies to be integrated via a modular approach. Integrating these
dimensions, including nonlinear threshold events such as mass migrations, or the outbreak of conflicts or
epidemics, is possible to a far greater degree with an ABM than with most other approaches.
We are developing a prototype ABM, implemented in Netlogo, to examine the dynamic impacts on poverty,
migration, mortality and conflict from climate change in Bangladesh from 2001 to 2100. The model employs
GIS and sub-district level census and economic data and a coarse-graining methodology to allow model
statistics to be generated on a national scale from local dynamic interactions. This approach allows a more
realistic treatment of distributed spatial events and heterogeneity across the country. The aim is not to
generate precise predictions of Bangladesh’s evolution, but to develop a framework that can be used for
integrated scenario exploration. This paper represents an initial report on progress on this project. So far the
prototype model has demonstrated the desirability and feasibility of integrating the different dimensions of
the complex adaptive system and, once completed, is intended to be used as the basis for a more detailed
policy-oriented model.

Farming fit? Dispelling the Australian agrarian myth.

Background: Rural Australians face a higher mental health and lifestyle disease burden (obesity, diabetes and
cardiovascular disease) than their urban counterparts. Our ongoing research reveals that the Australian farming
community has even poorer physical and mental health outcomes than rural averages. In particular, farm men and
women have high rates of overweightness, obesity, abdominal adiposity, high blood pressure and psychological
distress when compared against Australian averages. Within our farming cohort we observed a significant
association between psychological distress and obesity, abdominal adiposity and body fat percentage in the
farming population.
Presentation of hypothesis: This paper presents a hypothesis based on preliminary data obtained from an
ongoing study that could potentially explain the complex correlation between obesity, psychological distress and
physical activity among a farming population. We posit that spasmodic physical activity, changing farm practices
and climate variability induce prolonged stress in farmers. This increases systemic cortisol that, in turn, promotes
abdominal adiposity and weight gain.
Testing the hypothesis: The hypothesis will be tested by anthropometric, biochemical and psychological analysis
matched against systemic cortisol levels and the physical activity of the subjects.
Implications of the hypothesis tested: Previous studies indicate that farming populations have elevated rates of
psychological distress and high rates of suicide. Australian farmers have recently experienced challenging climatic
conditions including prolonged drought, floods and cyclones. Through our interactions and through the media it is
not uncommon for farmers to describe the effect of this long-term stress with feelings of ‘defeat’. By gaining a
greater understanding of the role cortisol and physical activity have on mental and physical health we may
positively impact the current rates of psychological distress in farmers.

Staying healthy : the case of cotton and cane growers in Australia

Farmers and fishers have always been exposed to the vagaries of climate and global economic forces. However, in recent years there has been an accumulation of factors which are having a particularly severe impact upon rural Australia. The global financial crisis has negatively affected commodity prices and the viability of some rural communities is under threat. There is evidence to suggest that climate change is already impacting adversely on many primary producers and their ability to farm using traditional methods. Furthermore, many parts of rural Australia are still experiencing the effects of long-term drought and associated problems. Together, these circumstances can rightly be conceived of as 'difficult times'. Key areas recently identified in a decline in mental health among farmers include: increasing isolation, ongoing drought, increased government regulations, and a widening of the schism between urban and rural Australians. While there is a body of literature on behaviour around illness in the context of the stress of ' difficult times', there is little on preventative behaviours in these circumstances. This chapter reports preliminary findings from an exploratory research projects that investigates the process by which farmers and fishers achieve and maintain good physical and mental health in the context of 'difficult times'. The research takes a multiple case study approach, with five Australian sites, each with a different industry base, representing communities undergoing 'difficult times'. This chapter focuses on two of the sites and data obtained from interviews with farmers in the cotton and sugar industries. It discusses the behavioural choices that they make to maintain good physical health and mental wellbeing. These include choices about nutrition, physical activity, social connections such as participation in community, social or farm-related groups, opportunities for relaxation and regular medical check-ups.

Feeding ecology of benthic invertebrates in an intermittently open estuary

Abundances of benthic invertebrates in the Hopkins River estuary were not affected by connection to the ocean, but by freshwater discharge and sediment properties. The bivalve Soletellina alba was identified as a suspension feeder. Invertebrate food sources were derived mainly from estuarine organic matter, but also contained marine and freshwater/terrestrial plants despite reduced river flow during drought conditions.

Mid-Holocene (4200 kyr BP) mass mortalities in Mauritius (Mascarenes): insular vertebrates resilient to climatic extremes but vulnerable to human impact

In the light of the currently increasing drought frequency and water scarcity on oceanic islands, it is crucial for the conservation of threatened insular vertebrates to assess how they will be affected. A 4000 yr old fossil assemblage in the Mare Aux Songes (MAS), southwest Mauritius, Mascarene Islands, contains bones of 100 000+ individual vertebrates, dominated by two species of giant tortoises Cylindraspis triserrata and C. inepta, the dodo Raphus cucullatus, and 20 other vertebrate species (Rijsdijk, Hume, Bunnik, Florens, Baider, Shapiro et al. (2009) Mid-Holocene vertebrate bone Concentration-Lagerstätte on oceanic island Mauritius provides a window into the ecosystem of the dodo (Raphus cucullatus). Quaternary Science Reviews 28: 14–24). Nine radiocarbon dates of bones statistically overlap and suggest mass mortality occurred between 4235 and 4100 cal. yr BP. The mortality period coincides with a widely recognized megadrought event. Our multidisciplinary investigations combining geological, paleontological and hydrological evidence suggests the lake was located in a dry coastal setting and had desiccated during this period. Oxygen isotope data from a Uranium-series dated stalagmite from Rodrigues, an island 560 km east of Mauritius, supports this scenario by showing frequently alternating dry and wet periods lasting for decades between 4122 and 2260 cal. yr BP. An extreme drought resulted in falling water-tables at MAS and elsewhere on the island, perhaps deprived these insular vertebrates of fresh water, which led to natural mass mortalities and possibly to extirpations. In spite of these events, all insular species survived until at least the seventeenth century, confirming their resistance to climatic extremes. Despite this, the generally exponential increase of combined human impacts on islands including loss of geodiversity, habitats, and stocks of fresh water, there will be less environmental safe-haven options for insular endemic and native vertebrates during future megadrought conditions; and therefore will be more prone to extinction.

Time for tea

Contemporary dance has only recently gained the capability of mapping imagery into 3D theatre environments that appear to move towards and away from the audiences. This capability radically expands the metaphoric content possible in dance work. This dance performance simply named “3D” combines contemporary dance with stereoscopic and video imagery. It was performed live within the Melbourne Fringe Festival at the No Vacancy Gallery at Federation Square from September 30 to October 2, 2011. The dance character is a Victorian woman living in a drought-stricken land who dreams of having enough water for a cup of tea. The stereoscopic (3d) images are metaphors for her hopes and dreams. It was performed and animated by Megan Beckwith and the sound design was by the international sound designer Jacques Soddell.

Time for tea

Contemporary dance has only recently gained the capability of mapping imagery into 3D theatre environments that appear to move towards and away from the audiences. This capability radically expands the metaphoric content possible in dance work. This dance performance simply named “3D” combines contemporary dance with stereoscopic and video imagery. The dance character is a Victorian woman living in a drought-stricken land who dreams of having enough water for a cup of tea. The stereoscopic (3d) images are metaphors for her hopes and dreams. It was performed and animated by Megan Beckwith and the sound design was by the international sound designer Jacques Soddell.

Visionnaire 2011

Visionnaire : A screening of postgraduate and masters films

Play ‘Crack the Sky’ - Steffen Hagen
The Priest - Benjamin Engeset
Sitcom Idiots Inc. - Mitchell McTaggart
Playing for it - Jacinta Adams, Alex Dance, Simone Berman, Esteban Ulloa, James Westland & Jarrod Watters
The boy who lived in the cemetery - Kerina Pereira, Emma Robinson, Tim Mitchell, Helin Kusman & Rebecca Drought
A Burger too far - Scott Burgess, Rhys Salmon, Karina McCowan, Tom McCann, Michael Hales & Aidan Truscott
Crimson Drive - Samantha Kuruvita, Shaun McFadyen, Vegard Dahle, Anja Aasheim, Shehan Vestrheim & Mikael Skramesto
Mikael - Kathleen Lynch, David McKinnar, David Davey, Alex Voltz, Gavin Juchnevicius & Shannon McFarland
A Slight Case of Death - David Laub, Tian Zhang, Janine Evans, Josh Horeau, Jacobo Arenas & Ben Teychenne
Neil - Courtney Gardiner, Rebecca Jacobs, Mark D’Alessandro, Jacob Williams, Trygve Nordhammer & Paul Mooney
Little Donnie Berner - Louise Walsh, Bhare Kesmaei, Matthew Skibicki, Krister Svensli & Nicholas Issell
Intervention - Georgie Thomas, Allison Flanagan, Allison Erlanger, Fredrik Waldeland, Torkild Ziegler & Brad Smith
Vicissitudes - Ben Mix, Sinead Lau, Claire Patterson, Joel Buncle, James Magree & Thomas Boarder