[Topografičeskaâ karta 1:42000]. XI-45, XI-46, X-45, X-46, [Vyborgskoj guber. uězda Kûmmenè].

1:42000.

[Topografičeskaâ karta 1:42000]. XI-49, XI-50, X-49, X-50, Vyb. gub., u. Stranda i Èurâpâ / snimali: Ivanov", snimal" i čertil" Filimonov".

1:42000.

[Topografičeskaâ karta 1:42000]. XI-51, XI-52, X-51, X-52, Vyborg. gub., Èurâpâ uĕzd.

1:42000.

[Topografičeskaâ karta 1:42000]. XI-53, XI-54, X-53, X-54, Vyborg. gub., Èurâpâ i Keksgol'mskìj uĕzd. / snimali i c̆ertili Mihajlov", Krasovskìj, Antonov" i Dubrovin".

[1:42000].

[Topografičeskaâ karta 1:42000]. XI-55, XI-56, X-55, X-56, Vyborg. gub., Keksgol'm. uĕzd.

[1:42000].

[Topografičeskaâ karta 1:42000]. XI-41, XI-42, X-41, X-42, [Vyborgskaâ gubernìâ].

[1:42000].

Cryptic mosaicism involving a second chromosome X in patients with Turner syndrome

The high abortion rate of 45,X embryos indicates that patients with Turner syndrome and 45,X karyotype could be mosaics, in at least one phase of embryo development or cellular lineage, due to the need for the other sex chromosome presence for conceptus to be compatible with life. In cases of structural chromosomal aberrations or hidden mosaicism, conventional cytogenetic techniques can be ineffective and molecular investigation is indicated. Two hundred and fifty patients with Turner syndrome stigmata were studied and 36 who had female genitalia and had been cytogenetically diagnosed as having "pure" 45,X karyotype were selected after 100 metaphases were analyzed in order to exclude mosaicism and the presence of genomic Y-specific sequences (SRY, TSPY, and DAZ) was excluded by PCR. Genomic DNA was extracted from peripheral blood and screened by the human androgen receptor (HUMARA) assay. The HUMARA gene has a polymorphic CAG repeat and, in the presence of a second chromosome with a different HUMARA allele, a second band will be amplified by PCR. Additionally, the CAG repeats contain two methylation-sensitive HpaII enzyme restriction sites, which can be used to verify skewed inactivation. Twenty-five percent (9/36) of the cases showed a cryptic mosaicism involving a second X and approximately 14% (5/36), or 55% (5/9) of the patients with cryptic mosaicism, also presented skewed inactivation. The laboratory identification of the second X chromosome and its inactivation pattern are important for the clinical management (hormone replacement therapy, and inclusion in an oocyte donation program) and prognostic counseling of patients with Turner syndrome.

De angelis imperiorum, ex Dan. X. 13, 20

Variantti A.

Antinociceptive effects of the essential oil of Mentha x villosa leaf and its major constituent piperitenone oxide in mice

Mentha x villosa Huds (Labiatae) is an aromatic herb widely used in folk medicine. Since the essential oil of the herb has many pharmacological activities, including antispasmodic effects, we determined whether the oil and its major constituent, piperitenone oxide (PO), have antinociceptive activity. The essential oil of M. x villosa (EOMV) and PO administered orally at 200 mg/kg (vehicle: 0.1% Tween 80 in water) significantly reduced the writhings induced by acetic acid from control values of 59.5 ± 3.1 s (N = 10) to 31.9 ± 2.8 s (N = 10) and 23.8 ± 3.4 s (N = 10), respectively. When administered at 100 and 200 mg/kg, EOMV reduced the paw licking time for the second phase of the formalin test from the control value of 20.6 ± 2.1 s (N = 13) to 5.3 ± 2.2 s (N = 12) and 2.7 ± 1.2 s (N = 18), respectively. At 100 and 200 mg/kg, PO reduced this second phase to 8.3 ± 2.7 s (N = 12) and 3.0 ± 1.2 s (N = 10), respectively. This effect of EOMV and PO was not reversed by naloxone. EOMV and PO had no significant effect on the first phase of the formalin test. As evaluated by the hot-plate and tail immersion test, EOMV and PO, at doses up to 200 mg/kg, showed no analgesic activity. These results show that EOMV and PO have antinociceptive activity and suggest that this effect is probably an indirect anti-inflammatory effect, which does not involve the central nervous system.

Mutations of Bruton's tyrosine kinase gene in Brazilian patients with X-linked agammaglobulinemia

Mutations in Bruton's tyrosine kinase (BTK) gene are responsible for X-linked agammaglobulinemia (XLA), which is characterized by recurrent bacterial infections, profound hypogammaglobulinemia, and decreased numbers of mature B cells in peripheral blood. We evaluated 5 male Brazilian patients, ranging from 3 to 10 years of age, from unrelated families, whose diagnosis was based on recurrent infections, markedly reduced levels of IgM, IgG and IgA, and circulating B cell numbers <2%. BTK gene analysis was carried out using PCR-SSCP followed by sequencing. We detected three novel (Ala347fsX55, I355T, and Thr324fsX24) and two previously reported mutations (Q196X and E441X). Flow cytometry revealed a reduced expression of BTK protein in patients and a mosaic pattern of BTK expression was obtained from mothers, indicating that they were XLA carriers.

De sortilegio, quam occasione verborum Cornelii Taciti Lib. de moribus Germanorum, cap. X

Gratulaatio: Johannes Flachsenius, David Lund, Gabriel Thauvonius, Nicol. Procopaeus, Gabr. Forssteen.

De judiciis mortalium circa fata magnorum virorum, ductu capitum IX. & X. lib. I. Annalium C. Cornelii Taciti suntomos congestus

Dedikaatio: Nicolaus Gyldenstålpe.

De authentia vocis kav, Psalm. XIX:5. hujusque convenientia cum voce fthóngos, a Sancto Paulo, ex LXX. interpretibus, adducta Rom. X:18, idoneis argumentis demonstrata

Nimekettä edeltää hepreankielinen invokaatio.

De angelis imperiorum, ex Dan. X. 13, 20

Variantti B.

Zelus vindex gratiae salvificae ordinatae, circa ruinam Israelis inordinati conspicuus, & ex cap. IX, X, XI. Epistulae ad Romanos delineatus disqvisitione inaugurali

Invokaatio: Cum Deo!