Influence of glyceryl guaiacolate ether on anesthetics in tilapia compared to benzocaine and eugenol

Objective. The study aimed to investigate the effectiveness of glyceryl guaiacolate ether (GGE) and compare the times of induction, recovery, hematological changes, total protein and glycaemia among anesthetics in Nile tilapia, Oreochromis niloticus. Materials and methods. A total of 60 tilapia distributed in 3 aquariums (N=20) were used, which formed the group benzocaine (100 mg/L), eugenol (50 mg/L) and guaiacol glyceryl ether (9.000 mg/L). After the induction of anesthesia fish blood samples were collected to determine the complete hemogram and glycemia. Then the animals were placed in aquariums with running water for assessing the anesthesia recovery. Results. It was verified that GGE showed longer induction and recovery times as well a significant increase (p<0.05) of glycemia, when compared with the other groups (p<0.05). The concentration of total protein did not differ between groups (p>0.05). An increase in the number of monocytes in the group treated with benzocaine (p<0.05) was observed in the analysis of the hematological parameters with no difference between groups for other variables. Conclusions. Eugenol and benzocaine allow rapid induction and recovery in Nile tilapia, without evidence of stress during handling and GGE showed high induction and recovery times, being inadequate for anesthetic use in Nile tilapia.

Low Dose of the Anesthetic Propofol Does Not Induce Genotoxic or Mutagenic Effects in Nile Tilapia

We demonstrate the efficacy of propofol (2,6-diisopropylphenol) as an anesthetic when administered to fish in an immersion bath and show the absence of genetic side effects following short-term exposure to the drug. All tested fish were anesthetized (as indicated by loss of posture and lack of response to physical stimulation), and both the comet assay (tail intensity) and the micronucleus assay revealed that propofol does not induce primary DNA damage or chromosome damage in the fish Nile Tilapia Oreochromis niloticus. Our results should be considered in light of our particular test conditions, including the water temperature (similar to 25 degrees C), the life stage and size of the fish, and the single exposure to the anesthetic. We suggest that propofol is a promising anesthetic in terms of its lack of genotoxic effects, at least in low dosages in adult Nile Tilapia.Received June 25, 2013; accepted October 15, 2013

Redox dysregulation and bioaccumulation of copper in tilapia Oreocromis niloticus

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Uso de complexo enzimático em dietas para tilápia do Nilo: digestibilidade, atividade enzimática, desempenho produtivo e parâmetros fisiológicos

Pós-graduação em Zootecnia - FCAV

Marcadores moleculares na identificação de híbridos e introgressão genética em populações de Pseudoplatystoma corruscans e Pseudoplatystoma reticulatim

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Crescimento compensatório em linhagens de tilápia do Nilo (Oreochromis niloticus L.) cultivadas em ambiente resfriado: morfologia muscular e expressão de genes relacionados ao crescimento muscular

Pós-graduação em Aquicultura - FCAV

Utilização de composto orgânico de peixe em adubação de capim-marandu

Pós-graduação em Zootecnia - FCAV

Microbiological quality of whole and filleted shelf-tilapia

Despite fish being a rich source of animal nutrients and having numerous associated health benefits, it is an extremely perishable food, prone to a wide range of hazards. The bacterial load associated with shelf-whole-fish organs (e.g. digestive tracts and skin) or mishandling of fish may be a vehicle of infection and become a risk to public health. The objective of this paper is to evaluate the microbiological quality of whole ungutted and filleted shelf-tilapia, as well as assess the safety for human consumption. For this purpose, in order to investigate the distribution and occurrence of bacterial populations, the count of total and thermotolerant coliforms, coagulase-positive Staphylococcus and presence of Salmonella spp. was determined. This paper shows that all fish organs were contaminated with thermotolerant coliform. Skin and fillet show higher populations and occurrence of all microorganisms analyzed. Lower bacterial populations were recovered from the gut and muscles of whole tilapia. Two samples of fillet were contaminated with coagulase-positive Staphylococcus. It can be concluded that the skin and filleted tilapia are important carriers of food-borne pathogens. In addition, fish might become an important cross and self-contamination source. (C) 2014 Elsevier B.V. All rights reserved.

Polyculture of frogs and tilapia in cages with high feeding frequency

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Sugar cane vinasse in water bodies: Impact assessed by liver histopathology in tilapia

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Non-specific immune parameters and physiological response of Nile tilapia fed beta-glucan and vitamin C for different periods and submitted to stress and bacterial challenge

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Substratum choice for nesting in male Nile tilapia Oreochromis niloticus

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Estudo da vazão específica nas bacias hidrográficas dos rios Aguapeí e Peixe como subsídio à gestão dos recursos hídricos

A área de estudo deste trabalho engloba as bacias hidrográficas dos rios Aguapeí e Peixe, localizadas a Oeste do Estado de São Paulo. Estas por sua vez estão vinculadas aos Comitês de Bacias Hidrográficas Aguapeí e Peixe (CBH-AP). Diante da iminência da implementação da política de cobrança pelo uso dos recursos hídricos nessa área, o presente trabalho apresenta em linhas gerais o estudo da vazão específica de forma que os resultados possam contribuir com os órgãos públicos de gestão a fim de que se execute uma gestão mais efetiva dos recursos hídricos. Com base em dados hidrológicos das estações fluviométricas inventariadas através do Sistema Integrado de Gestão de Recursos Hídricos do Estado de São Paulo (SIGRH) e da Agência Nacional de Águas (ANA) nas duas bacias hidrográficas, procedeu-se o estudo da vazão específica. Os resultados desses estudos apontam para uma maior variabilidade da vazão específica média na bacia hidrográfica do rio do Peixe, uma vez que ao longo da série histórica essa bacia apresenta maiores valores de vazão, foi possível também observar as mudanças no comportamento da vazão hidrológica nas duas bacias hidrográficas, ou seja, através dos três períodos hidrológicos identificados foi possível depreender que a vazão específica média em um período se mostra bem distribuída em ambas as bacias hidrográficas, em outro período hidrológico essa distribuição já se apresenta mais desigual. Tais informações possibilitam aos órgãos de gestão um planejamento mais refinado e eficiente

O uso da somatotropina bovina recombinante em reprodução bovina

The recombinant bovine somatotropin (rbST) at first, had its widespread use in dairy cows in order to increase milk production. Currently, it has been studied frequently and use their influence both in bovine milk, such as cutting. Its production has been an evolution to the science, using bacteria to produce recombinant DNA. Most authors that have studied and obtained positive results, such as increasing the number of ovarian follicles larger than five millimeters, among others. Its action takes place directly on the ovary, follicles, corpus luteum, the granulosa cells, oviduct, myometrium, endometrium and placenta, where they were found receptors, or indirectly through the release of insuline like growth factor-1 (IGF-1). Therefore, the objective of this work is to explain the importance of bST in bovine as well as the usefulness of this, its mechanism of action and the benefits it can bring when combined with other biotechnology, such as superovulation, embryo transfer, synchronization of estrus, and others

Biotecnologias aplicadas à reprodução de éguas velhas

The acceptance of biotechnology for the most equine breeders association had a significant effect in the horse industry, gaining popularity around the world, because the increasing on the genetic gain, allowing the use of sub fertile mares and stallions with high genetics value on reproduction. The embryos in vitro production of human and cattle has been used with success, however in vitro embryo production is not efficient in the horse, as oocyte transfer (OT) and intracytoplasmatic sperm injection (ICSI). The oocyte transfer has been used especially in subfertile old mares presenting reproductive pathologies as: endometrite, cervical and uterine adhesions, blocked oviduct, perineal laceration and ovulation failures. During oocyte recovery process, the oocytes must be collected from immature follicles that need be matured in vitro or in vivo matured oocytes from pre-ovulatory follicles through the transvaginal aspiration guided by ultrasound. The recovered oocyte is transferred to a previously inseminated recipient mare, through the flank laparotomy. The intracytoplasmatic sperm injection (ICSI) is a procedure of in vitro fertilization that needs only one sperm that is aspirated and injected inside the oocyte. The oocytes used, can be from mature and immature follicles. Fresh, cooled and frozen semen can be used, because the procedure not requires a functional sperm. The use of Piezo drill resulted in a breakthrough the pellucid zone, allowing the vibration per minute provided in the sperm injection pipette, a major result of cleaved oocytes, due to a better sperm injection in the oocyte. The embryo transfer can be straight inside the oviduct, as also transcervical transferred after embryo culture produced in vitro. In conclusion both procedures (OT and ICSI) are effective to be used on equine assisted reproduction, getting results even lower than expected, but satisfactory from animal genetically superior