Changes in biomass, chemical composition and nutritive value of Spartina alterniflora due to organic pollution in the Itanhaém River Basin (SP, Brazil)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

First record of Trypanosoma sp. (Protozoa: Kinetoplastida) in tuvira (Gymnotus aff. inaequilabiatus) in the Pantanal wetland, Mato Grosso do Sul State, Brazil

O objetivo do presente estudo foi reportar a infecção por Trypanosoma sp. em tuviras (Gymnotus aff. inaequilabiatus) oriundas do Pantanal Sul-mato-grossense, Brasil. Dez peixes provenientes do rio Paraguai, Pantanal Sul-mato-grossense, foram avaliados quanto à presença de hemoflagelados. Tripomastigotas de Trypanosoma sp. foram observados nas extensões sanguíneas de três peixes (30% de prevalência), e algumas formas encontravam-se em divisão. Por meio do exame a fresco e da centrifugação do sangue em capilar de hematócrito como métodos para diagnóstico, a taxa de prevalência foi de 80%. Esse é o primeiro relato de Trypanosoma sp. em tuviras no Brasil.

Avaliação da composição química, atividade antioxidante, antibacteriana, antinoceptiva, antiinflamatória e toxicidade do extrato metanólico e frações de folhas de Spondias sp. (Anacardiaceae)

Spondias sp. (Anacardiaceae), popularly known as cajá-umbu, is an endemic plant from Northeastern Brazil, where their leaves are widely used in folk medicine to treat inflammatory processes, while their fruits have a great agro industrial potential. This study was designed to evaluate hepatoprotective, antinociceptive, antioxidant, antimicrobial and anti-inflammatory properties, as well as the acute toxicity and repeated dose 28, using a methanolic extract (MES), a fraction rich in flavonoids (FRF) and a precipitate from Spondias sp.leaves. The antioxidant activity of them was valued to evaluate their free radical scavenger capacity by DPPH test, whereas MES and FRF were used to evaluate while the preventive action on carbon tetrachloride (CCl4)-induced hepatotoxicity. Seven groups (n=5) of female Wistar rats were used as follows: control group, CCl4-intoxicated group treated with EMS (500 mg/kg) for 7 days, three CCl4-intoxicated groups treated with FRF (25, 50 and 75 mg/kg) for 7 days and the CCl4-intoxicated group treated with Legalon ® (silimarina; (phytotherapeutic reference) (50 mg/kg; 7 days). MES and FRF showed a protective action against liver injury induced by CCl4, being observed a significant reduction of serum enzyme activity marker of liver damage (alanine transaminase and aspartate transaminase). On the other hand, the lipid peroxidation (SRAT) decrease, as well as the increase of glutathione content and enzyme activity of antioxidant defense system (SOD, CAT, GPx) toward near normal values indicated the ability of EMS to restore the oxidative imbalance induced by CCl4. The histological analysis confirmed the hepatoprotection, compared to degenerative changes in CCl4-treated group. This hepatoprotetor effect was similar to that shown by Legalon®. The in vitro high antioxidant capacity of extract (93.16 ± 1.00%) showed analogous results to those obtained by Carduus marianus BHT (reference standard). This fact explains the obtained results in vivo. Although no antimicrobial activity was detected, EMS and FRF promoted the antinociceptive effect induced in the second phase by the intraplantar formalin test, evidencing the anti-inflammatory action; confirmed by the carrageenan-induced peritonitis model. The evaluation of the mechanical allodynia (CFA a 80%) demonstrated the involvement of the Spondias sp. chemical composition in the anti-inflammatory activity toward the acute processes. The acute exposure and repeated dose during 28 days did not produce significant changes in the parameters that evaluate toxicity. Together the experimental results reveal, that Spondias sp. leaf extracts have a promising potential in pharmaceutical area, and due to its non-toxic condition present efficiency and security

Levantamento de Meloidogyne exigua na cultura da seringueira em São José do Rio Claro, MT, Brasil

Este trabalho foi realizado com o objetivo de efetuar um levantamento da ocorrência de Meloidogyne exigua em seringueira em São José do Rio Claro, MT. Foram amostradas 191 propriedades agrícolas, totalizando cerca de 18.000ha. Os nematóides foram identificados no Laboratório de Nematologia do Departamento de Fitossanidade da FCAV/UNESP, em Jaboticabal, SP. Foram encontrados níveis populacionais de M. exigua entre 0 e 61.824 juvenis/5g de raízes.

Indução da expressão precoce de sintomas de Guignardia citricarpa em frutos de laranjeira 'pêra-rio'

O presente trabalho teve por objetivo avaliar a influência de diferentes concentrações de ethephon na expressão precoce de sintomas de Guignardia citricarpa em frutos de laranjeira 'Pêra-Rio'. Para tal, frutos assintomáticos e isentos de aplicações com fungicidas, com 20 e 28 semanas após a queda de pétalas, foram coletados em área de comprovada existência da doença, no município de Conchal-SP e levados ao Laboratório de Fitopatologia da FCAV/UNESP, em Jaboticabal-SP, onde foram tratados com soluções nas seguintes doses de ethephon: i) 1,57 g L-1; ii) 2,10 g L-1; iii) 2,42 g L-1; iv) Testemunha (água). Todas acrescidas de imazalil a 0,25 g L-1, para prevenir podridões de pós-colheita. Após os tratamentos, os frutos foram mantidos em câmara incubadora para B.O.D., calibrada à temperatura de 25ºC ±1ºC, por 15 dias. Posteriormente, os frutos foram submetidos a quatro avaliações, em intervalos semanais, sendo atribuídas notas que variaram de zero (ausência de sintomas) a 6 (sintomas severos). Os dados da severidade da doença observados nos frutos colhidos prematuramente e submetidos aos diferentes tratamentos com ethephon foram comparados aos observados em frutos ensacados e não ensacados, mantidos no campo até a maturação natural. Constatou-se maior equivalência de sintomas nos frutos com idade entre 20 e 28 semanas, quando estes foram tratados com 2,10 g L-1 de ethephon e avaliados entre 28 e 35 dias. Concluiu-se que o emprego de ethephon, nestas condições, viabilizou a expressão precoce dos sintomas da mancha preta em frutos contendo infecções quiescentes de G. citricarpa, com antecedência de, pelo menos, 105 dias antes da colheita. Tal resultado constitui-se, portanto, em alternativa de grande aplicabilidade na detecção precoce de sintomas de mancha preta.

Programa alfabetização solidária: um estudo sobre a experiência no município de Lagoa de Pedras/RN (1997 - 2003)

The educational reform of the 90 s was tainted by the objectives of the fiscal adjustments, resulting in the redefinition of the state s role in the financing and offering of teaching services, and bringing about a shuffling of the responsibility between the public and private sectors to promote education to young people and adults. The 90 s also highlighted the proliferation of providers and the multiplication of Educational Programs for Youths and Adults (EJA), implemented through partnerships between governmental and nongovernmental agencies. During this period of time, the agenda of educational responsibilities concerning analphabetism was organized in a process of decentralized of the state, with the following political, social, and economic objectives: to reduce the public deficit, increasing public savings and the financial capacity of the state to concentrate resources in areas considered indispensable to direct intervention; to increase the efficiency of the social services moffered or funded by the state, giving citizens more at a lower cost, and spreading services to more remote areas, expanding access to reach those most in need; to increase the participation of citizens in public management, stimulating communitarian acts as well as developing efforts towards the effective coordination of public figures in the implementation of associated social services. Thus, Assistance Programs co-financed by the government try to deal with the problem of analphabetism. Within the sphere of the 90 s educational policy decentralization, we come to see how the agenda dedicated to the reduction of analphabetism was formed by the Solidarity Alphabetization Program (PAS). Between 1997 and 2003, the latter agenda s decentralizing proposal was integrated in the management partnership for the operationalization of tasks and resources faced with the execution of the formal objectives. In this study, we identify the dimensions of the implantation and progress of the tasks carried out by PAS, in the municipality of Lagoa de Pedras/RN. However, we consider these Programs to assist in the process without guaranteeing the reduction of the causes or substituting the responsibility of the system once the monetary resources for program maintenance provided by the partners is exhausted

Sensoriamento remoto e geoprocessamento aplicados ao uso da terra em microbacias hidrográficas, Botucatu - SP

O presente trabalho teve como objetivo identificar e quantificar o uso da terra em dez microbacias ocorrentes na bacia do Rio Capivara, município de Botucatu - SP, a partir da estruturação de um banco de dados utilizando o Sistema de Informações Geográficas (SIG) - IDRISI. Os resultados mostram que as classes de uso da terra, uso agrícola e pastagem, foram as mais significativas, pois ocuparam mais da metade da área das microbacias. O alto índice de uso da terra por pastagens, capoeiras, reflorestamento e matas reflete a predominância de solos arenosos com baixa fertilidade. As imagens obtidas do satélite LANDSAT 5 permitiram o mapeamento do uso da terra de maneira rápida, além de fornecer um excelente banco de dados para futuro planejamento e gerenciamento das atividades agropecuárias regionais. O SIG-IDRISI permitiu identificar, por meio de seus diferentes módulos para georreferenciamento, classificação digital e modelo matemático, as classes de uso da terra com rapidez.

Fatores ambientais na distribuição de populações de Brachiaria subquadripara presentes no Reservatório de Barra Bonita-SP

Este estudo teve como objetivos monitorar a distribuição de populações de Brachiaria subquadripara e caracterizar seu ambiente de ocorrência em duas épocas (seca e águas). As coletas foram realizadas em julho de 2004 e janeiro de 2005 em pontos previamente selecionados e georreferenciados, os quais se constituíram de 13 pontos no reservatório Barra Bonita (sete pontos no braço do rio Piracicaba e seis no braço do rio Tietê). Foram realizadas coletas de solo nas margens dos rios, sedimento, água e plantas. A distribuição em relação à área de infestação das populações de B. subquadripara observada na estação chuvosa foi influenciada pela variação sazonal, a qual ocorreu de forma heterogênea em relação à densidade populacional. O rio Tietê foi considerado um ambiente mais eutrófico do que o rio Piracicaba tanto em relação ao solo quanto em relação à coluna d'água. As amostras de água coletadas no rio Tietê apresentaram valores médios de pH na estação seca de 7,02 a 7,82, e na estação das águas registrou-se maior amplitude de variação (6,80 a 8,0). A maioria dos pontos amostrados apresentou altos teores de matéria orgânica no sedimento, sendo observados teores menores que 1,3% em dois pontos de coleta na época da seca e em cinco pontos na época das águas.

Biossorção utilizando alga marinha (sargassum sp.) aplicada em meio orgânico

In this work, biosorption process was used to remove heavy metals from used automotive lubricating oils by a bus fleet from Natal-RN-Brazil. This oil was characterized to determine the physical-chemistry properties. It was also characterized the used oil with the aim of determining and quantifying the heavy metal concentration. Fe and Cu were the metals existent in large concentration and these metals were choused to be studied in solubilization process. For the biosorption process was used the seaweed Sargassum sp for the study of influencing of the metals presents separately and with other metals. It was also studied the effect of the protonation treatment of alga with the objective to know the best efficiency of heavy metals removal. The study of the solubilization showed that the presence of more than a metal favors the solubilization of the metals presents in the oil and consequently, it favors the biosorption process, what becomes interesting the perspective application in the heavy metals removal in lubricating oils used, because the presence of more than a heavy metal favors the solubility of all metals present. It was observed that the iron and copper metals, which are present in large concentration, the protonated biosorbtent was more effective. In this study we used as biomass the marine alga Sargassum sp to study the influence of agitation velocity, temperature and initial biomass concentration on the removal of iron and copper from used lubricant oils. We performed an experimental design and a kinetic study. The experiments were carried out with samples of used lubricant oil and predetermined amounts of algae, allowing sufficient time for the mixture to obtain equilibrium under controlled conditions. The results showed that, under the conditions studied, the larger the amount of biomass present, the lower the adsorption capacity of the iron and of the copper, likely due to a decrease in interface contact area. The experimental design led us to conclude that a function can be obtained that shows the degree of influence of each one of the system variables

A presença da produção científica em psicologia na formação de psicólogos do Rio Grande do Norte

The present research aimed to analyze the presence of national scientific production in undergraduate studies in Psychology of Rio Grande do Norte. Therefore, the bibliographical references contained in education plan of subjects linked to the common center of the courses, which were taught in 2011 in three Psychology courses of the state, were focused. The analysis of the material was based on bibliographical and contextual aspects of the production, such as year, nationality, type of material, authorship (including the link of authors to PPGs), structuring axis of the subject in which it was mentioned, among others. The results showed that the national production is predominant in all courses, and they are, mostly, originated from PPGs of Psychology. Among these, the publications resulted from programs in Southwest, with emphasis to PUC-SP, are recent (mostly from 2000s) and they are predominantly composed by books (organizations and full texts). Regarding the distribution of Psychology PPGs production by the structuring axes, it is observed that it surpasses the foreign production only in axis C (procedures of scientific investigation and professional practice) and axis F (professional practices). Accordingly, it is concluded that Psychology production is found in graduation, sharing space with foreign publications and from other areas of knowledge. On the one hand, this is positive, by taking into account the importance of several foreign works and from other fields to build the knowledge in Psychology; on the other hand, it shows that the production from this area is inserted in graduation in a less significant way than its growth, which points out the existence of gaps in the development of some investigative domains from national Psychology

Extração e análise da fração lipídica da microalga monoraphidium sp., síntese e caracterização do seu biodiesel

The cultivation of microalgae biomass in order to produce biodiesel arises as an extremely promising aspect, in that the microalgae culture includes short cycle of reproduction, smaller areas for planting and residual biomass rich in protein content. The present dissertation evaluates the performance and features, through spectrometry in the region of infrared with transformed Fourier (FTIR) and spectrometry in the region of UVvisible (UV-Vis), of the extracted lipid material (LM) using different techniques of cell wall disruption (mechanical agitation at low and at high spin and agitation associated with cavitation). The technique of gas chromatography (GC) brought to light the success of alkaline transesterification in the conversion of oil into methyl monoesters (MME), which was also analyzed by spectroscopic techniques (FTIR, proton magnetic resonance (1H NMR) and carbon (13C NMR). Through thermogravimetric analysis (TGA) were analyzed the lipid material (LM), biodiesel and the microalgae biomass. The method which provided the best results concerning the efficiency in extraction of the LP of Monoraphidium sp. (12,51%) was by mechanical agitation at high spin (14 000 rpm), for 2 hours being the ideal time, as shown by the t test. The spectroscopic techniques (1H NMR, 13C NMR and FTIR) confirmed that the structure of methyl monoesters and the chromatographic data (CG) revealed a high content of saturated fatty acid esters (about 70%) being the major constituent eicosanoic acid (33,7%), which justifies the high thermal stability of microalgae biodiesel. The TGA also ratified the conversion rate (96%) of LM into MME, pointing out the quantitative results compatible with the values obtained through GC (about 98%) and confirmed the efficiency of the extraction methods used, showing that may be a good technique to confirm the extraction of these materials. The content of LM microalgae obtained (12,51%) indicates good potential for using such material as a raw material for biodiesel production, when compared to oil content which can be obtained from traditional oil for this use, since the productivity of microalgae per hectare is much larger and requires an extremely reduced period to renew its cultivation

Equilíbrio postural e risco para queda em idosos da cidade de Garça, SP

OBJETIVOS: Avaliar o equilíbrio postural e o risco de quedas em idosos institucionalizados e não-institucionalizados. MÉTODOS: Foram incluídos no estudo 45 idosos institucionalizados e 43 idosos não-institucionalizados. O equilíbrio e o risco de quedas foram avaliados através da Escala de Equilíbrio de Berg. A comparação do equilíbrio entre os grupos foi feita por meio do teste de Mann-Whitney. Foi avaliado o risco para queda entre as populações. Adotou-se um valor de p ≤ 0,05 como significante. RESULTADOS: Houve diferença entre os escores da Escala de Berg nas populações estudadas (p < 0,0001). Os resultados mostraram que a população institucionalizada apresenta nove vezes mais risco de cair do que idosos da comunidade (p=0,0001). CONCLUSÕES: Os resultados indicam que o equilíbrio postural é menor na população institucionalizada, aumentando seu risco de cair.

Sobre pedras, entre rios: modernização do espaço urbano de Caicó/RN (1950/1960)

thèse de maîtrise, qui a été idealizée et exécutée à partir de la réunion et les relations dialogiques établies entre la géographie et l'histoire, et l'espace et le temps, a considéré l'environnement urbain comme le thème général et, comme la référence empirique, l'espace urbain de Caicó, incrusté dans les terres semi-arides du Sertão do Seridó Potiguar, plus précisément à la mi-sud de l'État du Rio Grande do Norte. Dans cet espace, à travers de recherche des fragments de mémoires acquis de diverses sources historiques, on a tenté de faire des investigations sur les transformations urbaines qui ont eu lieu dans la ville au cours des années 50 et 60 du XXe siècle. Ces transformations, dans l espace urbain de Caicó à un moment du pic de l'activité de développement de coton, reflétaient et conditionnaient les propres projets de modernization urbaine qui représentants des élites locales eurent conçu avec l'objectif de la construction d une ville idéal dans les regions isolées du Seridó: moderne, civilisé, progressive et capitale régionale du Seridó. Pour cette construction, les nouveaux équipements et services urbains ont passé du plan imaginaire à l espace réel, installés dans plusieurs quartiers de la ville en transformant le paysage urbain. Face à cette situation, cinémas, magasins, station de radio, énergie électrique, institutions de l'éducation, téléphonie, maison de soins infirmiers, usine de bénéficiement de coton, banques, politiques urbaines, hygiéniques et sanitaires, modes de comportement, sociabilités urbaines distincttes, parmi d'autres éléments géographiques se sont institués à travers la réalisation de certains idéaux de progrès social et dans un esprit de modernité urbaine, dans de nouveaux espaces et pratiques inventés, tramés et éprouvés sur les rochers et entre le cours d eau au milieu d'un quotidien urbain marqué par ruptures et permanence de certaines coutumes et habitudes antiques et de certains paysages et environnement ridé

First Autochthonous Case of Canine Visceral Leishmaniasis in the Center of Rio Grande do Sul State, Brazil

Background: Leishmaniasis is one of the most important vector-borne diseases of humans. This parasitic disease can be caused by many species of Leishmania. In humans, different species of the parasite are associated with different forms of the disease, cutaneous and visceral. Among domesticated animals, dogs are the most important species in the epidemiology of this disease. Leishmania chagasi, an important zoonosis, is well established as the agent of visceral leishmaniasis in Brazil. The disease is endemic in north, northeast, midwest and southeast, and is transmitted to mammals by hematophagous insects such as the Lutzomyia longipalpis. In 2008, our research group has diagnosed a case of canine leishmaniasis in the municipality of Uruguaiana and subsequently there were several cases in the city and the neighbor municipality of Sao Borja. Most Brazilian states are endemic for leishmaniasis, with the exception of Rio Grande do Sul. In southern Brazil, the reports of humans and dogs infected by Leishmania spp. are the source of endemic area in the country. Therefore, the aim of this study is register the first clinical case of canine visceral leishmaniasis in the municipality of Santa Maria, RS.Case: In october 2010, a veterinary clinic of Santa Maria received a canine, female, Doberman, with two years of age. The animal had severe skin lesions on the head and limbs, pale mucous membranes, and enlarged lymph nodes. According to the owner, the animal showed progressive weight loss and anorexia for more than five days. During the clinical examination the blood was collected for hemogram and cytology of lymph nodes was performed by puncture aspiration with a fine needle. In the erythrogram, it was observed a decrease in the total number of erythrocytes (2.8 x 10(6)/mu L), hematocrit (21%), hemoglobin (6.8 g/dL) and platelets (98 x 10(3)/mu L). In the leucogram, any alteration was observed. The cytology of lymph nodes showed amastigotes forms, suggestive of the Leishmania spp. Based on this finding; we performed the blood collection for PCR, to confirm parasitism and to determine the species of Leishmania. At the molecular test was used PCR-specific for L. chagasi, and the result was positive.Discussion: This is the first autochthonous clinical case in the central region of the RS, non-endemic area for leishmaniasis. In serological studies of visceral leishmaniasis it was diagnosed in five asymptomatic dogs in the municipalities of Santa Maria, Julio de Castilhos and Itaara, however not confirmed by molecular analysis. In the municipalities of Cruz Alta and Uruguaiana cases of L. chagasi have been reported in dogs which previously resided in Leishmania sp. endemic areas. The municipality of Sao Borja had the first record of L. longipalpis in the RS during the leishmaniasis outbreak in 2008-2009. In the central region of the RS vector has not been found, but because in this first autochthonous case dog in Santa Maria believe that the parasite is present and/or doing other insect transmission of leishmaniasis. Clinical signs associated with hematologic and coagulation disorders observed in the canine are commonly described in symptomatic dogs in endemic regions. This case of autochthonous leishmaniasis reinforces the idea of the vector presence in Santa Maria, center of the RS. We believe that canine leishmaniasis is an emerging disease in the southern region of Brazil.

Porcentagens de saponinas e taninos em vinte e oito cultivares de alfafa (Medicago sativa L.) em duas épocas de corte - Botucatu - SP

O objetivo do estudo foi determinar os conteúdos de saponinas, taninos e a solubilidade da proteína (SP) de 28 cultivares de alfafa: Crioula, Monarca, BR 4, Alto Great, MH 4, SW 9210 A, 5929, BR 1, EL Grande, 5715, MH 15, Valley Plus, BR 2, Rio, SW 8210, Maricopa, ICI 990, 5888, P 30, Alfa-200, WL 516, SW 8112 A, BR 3, Florida 77, Araucana, Falcon, Semit 921 e Sutter. O material analisado foi obtido do 10º e 14º corte, respectivamente; em 08/12/97 e 16/04/98, de um experimento desenvolvido na Faculdade de Medicina Veterinária e Zootecnia, UNESP, Botucatu. Amostras de cada cultivar foram colhidas, pesadas e secas em estufa a 52ºC. As saponinas foram extraídas com solvente hidrofílico e lipofílico e o conteúdo foi calculado pela equação y=0,8121x-1,4759, R² = 1,00. A extração dos taninos totais e condensados foi efetuada por meio de ultra-som (12 min), sendo os taninos totais determinados pela equação: y=44,978 + 0,5644 com R²=0,9977 e os condensados, multiplicando-se a absorbância por 78,26 dividido pelo teor de matéria seca. A SP foi determinada pelo método de KOH, de acordo com a seguinte fórmula: SP (%) = proteína solúvel x 100/proteína bruta da amostra. Os teores de saponinas, taninos totais e condensados e a solubilidade da proteína não diferiram (P>0,05) entre as cultivares. Houve efeito (P<0,05) da época de corte apenas sobre o teor de taninos totais. Os teores médios de saponinas de 1,00% aliados à baixa solubilidade média da proteína bruta (34,11%) não se constituem em fatores limitantes para uso dos cultivares de alfafa estudadas.