Physicochemical characterisation of protein ingredients prepared from milk by ultrafiltration or microfiltration for application in formulated nutritional products

Formulated food systems are becoming more sophisticated as demand grows for the design of structural and nutritional profiles targeted at increasingly specific demographics. Milk protein is an important bio- and techno-functional component of such formulations, which include infant formula, sports supplements, clinical beverages and elderly nutrition products. This thesis outlines research into ingredients that are key to the development of these products, namely milk protein concentrate (MPC), milk protein isolate (MPI), micellar casein concentrate (MCC), β-casein concentrate (BCC) and serum protein concentrate (SPC). MPC powders ranging from 37 to 90% protein (solids basis) were studied for properties of relevance to handling and storage of powders, powder solubilisation and thermal processing of reconstituted MPCs. MPC powders with ≥80% protein were found to have very poor flowability and high compressibility; in addition, these high-protein MPCs exhibited poor wetting and dispersion characteristics during rehydration in water. Heat stability studies on unconcentrated (3.5%, 140°C) and concentrated (8.5%, 120°C) MPC suspensions, showed that suspensions prepared from high-protein MPCs coagulated much more rapidly than lower protein MPCs. β-casein ingredients were developed using membrane processing. Enrichment of β-casein from skim milk was performed at laboratory-scale using ‘cold’ microfiltration (MF) at <4°C with either 1000 kDa molecular weight cut-off or 0.1 µm pore-size membranes. At pilot-scale, a second ‘warm’ MF step at 26°C was incorporated for selective purification of micellised β-casein from whey proteins; using this approach, BCCs with β-casein purity of up to 80% (protein basis) were prepared, with the whey protein purity of the SPC co-product reaching ~90%. The BCC ingredient could prevent supersaturated solutions of calcium phosphate (CaP) from precipitating, although the amorphous CaP formed created large micelles that were less thermo-reversible than those in CaP-free systems. Another co-product of BCC manufacture, MCC powder, was shown to have superior rehydration characteristics compared to traditional MCCs. The findings presented in this thesis constitute a significant advance in the research of milk protein ingredients, in terms of optimising their preparation by membrane filtration, preventing their destabilisation during processing and facilitating their effective incorporation into nutritional formulations designed for consumers of a specific age, lifestyle or health status

Recherche d’alternatives aux nitrates et nitrites dans les produits carnés

L’objectif principal de cette maîtrise était de développer des formulations d’épices capables de remplacer les nitrites et nitrates dans les produits carnés tout en gardant la même durée de conservation que ces derniers ainsi que leurs propriétés organoleptiques, antibactériennes et antioxydantes. Un criblage qualitatif puis quantitatif des épices a été réalisé et trois épices ont été sélectionnées, les clous de girofle, le cumin et la cannelle. Puis, des poudres de fruits ont été testées pour approcher la couleur rosée que donnent les échantillons contenant les nitrites. L’analyse sensorielle effectuée par la suite a fait ressortir le caractère sucré de la poudre de fruit et des propriétés très intéressantes de certaines formulations par rapport à l’ajout des nitrites. Enfin, une analyse technico-économique a été réalisée et a montré une très faible augmentation du coût de production des produits carnés en utilisant nos formulations d’épices et de poudre de fruit.

Enhancing the total phenolic content and antioxidants of lemon pomace aqueous extracts by applying UV-C irradiation on the dried powder

Several studies have shown that UV-C irradiation promotes the bioactive compounds and antioxidants of fresh fruits and vegetables. The aim of this study was to apply UV irradiation in lemon pomace dried powder for enhancing its phenolic content and antioxidant properties, thus more bioactive compounds should be available for extraction and utilization. Lemon pomace dried powder was placed above the UV lamp and treated with dosages of 4, 19, 80 and 185 kJ m-2, while untreated powder was used as a control. UV-C irradiation significantly affected the total phenolic content, total flavonoid content, proanthocyanidins and antioxidant capacity measured by CUPRAC and FRAP of the lemon pomace dried powder, while it did not affect the vitamin C content. UV-C irradiation of 19 kJ m-2 resulted in 19% higher total phenolic content than the control, while UV-C irradiation of 180 kJ m-2 resulted in 28% higher total flavonoid content than the control. The antioxidant capacity was reduced when UV-C irradiation more than 4 kJ m-2 was applied. The results of this study indicate that UV-C treatment has the potential to increase the extraction of bioactive compounds of lemon dried pomace at relatively high dosages.

Obtenção de concentrado proteico do farelo de arroz e avaliação das propriedades químicas e funcionais

Esta dissertação é composta por 5 artigos.

Extended formulations, nonnegative factorizations, and randomized communication protocols

An extended formulation of a polyhedron P is a linear description of a polyhedron Q together with a linear map π such that π(Q)=P. These objects are of fundamental importance in polyhedral combinatorics and optimization theory, and the subject of a number of studies. Yannakakis’ factorization theorem (Yannakakis in J Comput Syst Sci 43(3):441–466, 1991) provides a surprising connection between extended formulations and communication complexity, showing that the smallest size of an extended formulation of $$P$$P equals the nonnegative rank of its slack matrix S. Moreover, Yannakakis also shows that the nonnegative rank of S is at most 2^c, where c is the complexity of any deterministic protocol computing S. In this paper, we show that the latter result can be strengthened when we allow protocols to be randomized. In particular, we prove that the base-2 logarithm of the nonnegative rank of any nonnegative matrix equals the minimum complexity of a randomized communication protocol computing the matrix in expectation. Using Yannakakis’ factorization theorem, this implies that the base-2 logarithm of the smallest size of an extended formulation of a polytope P equals the minimum complexity of a randomized communication protocol computing the slack matrix of P in expectation. We show that allowing randomization in the protocol can be crucial for obtaining small extended formulations. Specifically, we prove that for the spanning tree and perfect matching polytopes, small variance in the protocol forces large size in the extended formulation.

Tipos de adubação para a videira 'BRS - violeta' cultivada em latossolo vermelho no sudoeste do Paraná

The vineyard culture, in the Southwest of Paraná, has faced an evolution in recently years. However, some technical barriers has contained the expression of its full potential. Among which, the lack of scientific and technical support about the fertilization management and the fertility maintenance of production fields, is the most worrying. This fact, allied with the raising on the consumers demand for ecological correct products, are the motivations for the present study, whose main objective was to evaluate the effects of different fertilizer formulations, based on alternative nutrient sources, on grapevine yield and grape fruit quality, aiming at the improvement of those parameters and the maintenance of soil fertility. To achieve this goal, an experiment has been evaluated since 2008, at the experimental area of the Federal Technological University of Paraná, Campus Pato Branco, where ten treatments were being tested, combining or isolating shale derivates from other alternative nutrient sources. Those are the treatments: T1: Gafsa Rock Phosphate (GRP) + K2SO4; T2: GRP + RPB (Rock Powder Bioland®); T3: GRP + K2SO4 + LH (Laying Hen Litter); T4: GRP+ RPB + LH; T5: GRP + K2SO4 + MBR (Matrix Shale 3); T6: GRP + RPB + MBR; T7: GRP + K2SO4 + MBR + LH; T8: GRP + RPB + LH + MBR; T9: TSP (Triple Superphosphate) + Urea + KCl and T10: absolute control. The usage of fertilization, specially the alternative fertilization, improved soil fertility characteristics and also the yield on the last two evaluated harvests. The potassium sulfate improved the potassium availability on soil, while improved yield on the last three harvests. The MBR improved the phosphorus availability, improved post-harvest conservation and improved the yield on the last evaluated harvest.

Incorporação de resíduos cerâmicos vidrados em argamassas bastardas: estudo de propriedades físicas e mecânicas

Dissertação de Natureza Científica para obtenção do grau de Mestre em Engenharia Civil Perfil de Edificações

A Multi-Objective Approach for VNE: Problems using multiple ILP formulations

Network Virtualization is a key technology for the Future Internet, allowing the deployment of multiple independent virtual networks that use resources of the same basic infrastructure. An important challenge in the dynamic provision of virtual networks resides in the optimal allocation of physical resources (nodes and links) to requirements of virtual networks. This problem is known as Virtual Network Embedding (VNE). For the resolution of this problem, previous research has focused on designing algorithms based on the optimization of a single objective. On the contrary, in this work we present a multi-objective algorithm, called VNE-MO-ILP, for solving dynamic VNE problem, which calculates an approximation of the Pareto Front considering simultaneously resource utilization and load balancing. Experimental results show evidences that the proposed algorithm is better or at least comparable to a state-of-the-art algorithm. Two performance metrics were simultaneously evaluated: (i) Virtual Network Request Acceptance Ratio and (ii) Revenue/Cost Relation. The size of test networks used in the experiments shows that the proposed algorithm scales well in execution times, for networks of 84 nodes

Similarity between the in vitro activity and toxicity of two different fungizone™ / lipofundin™ admixtures

Amphotericin B (AmB), an antifungal agent that presents a broad spectrum of activity, remains the gold standard in the antifungal therapy. However, sometimes the high level of toxicity forbids its clinical use. The aim of this work was to evaluate and compare the efficacy and toxicity in vitro of Fungizon™ (AmB-D) and two new different AmB formulations. Methods: three products were studied: Fungizon™, and two Fungizon™ /Lipofundin™ admixtures, which were diluted through two methods: in the first one, Fungizon™ was previously diluted with water for injection and then, in Lipofundin™ (AmB-DAL); the second method consisted of a primary dilution of AmB-D as a powder in the referred emulsion (AmB-DL). For the in vitro assay, two cell models were used: Red Blood Cells (RBC) from human donors and Candida tropicallis (Ct). The in vitro evaluation (K+ leakage, hemoglobin leakage and cell survival rate-CSR) was performed at four AmB concentrations (from 50 to 0.05mg.L-1). Results: The results showed that the action of AmB was not only concentration dependent, but also cellular type and vehicle kind dependent. At AmB concentrations of 50 mg.L-1, although the hemoglobin leakage for AmB-D was almost complete (99.51), for AmB-DAL and AmB-DL this value tended to zero. The p = 0.000 showed that AmB-D was significantly more hemolytic. Conclusion: The Fungizon™- Lipofundin™ admixtures seem to be the more valuable AmB carrier systems due to their best therapeutic index presented