Eukaryotic methionyl aminopeptidases: two classes of cobalt-dependent enzymes.

Using partial amino acid sequence data derived from porcine methionyl aminopeptidase (MetAP; methionine aminopeptidase, peptidase M; EC 3.4.11.18), a full-length clone of the homologous human enzyme has been obtained. The cDNA sequence contains 2569 nt with a single open reading frame corresponding to a protein of 478 amino acids. The C-terminal portion representing the catalytic domain shows limited identity with MetAP sequences from various prokaryotes and yeast, while the N terminus is rich in charged amino acids, including extended strings of basic and acidic residues. These highly polar stretches likely result in the spuriously high observed molecular mass (67 kDa). This cDNA sequence is highly similar to a rat protein, termed p67, which was identified as an inhibitor of phosphorylation of initiation factor eIF2 alpha and was previously predicted to be a metallopeptidase based on limited sequence homology. Model building established that human MetAP (p67) could be readily accommodated into the Escherichia coli MetAP structure and that the Co2+ ligands were fully preserved. However, human MetAP was found to be much more similar to a yeast open reading frame that differed markedly from the previously reported yeast MetAP. A similar partial sequence from Methanothermus fervidus suggests that this p67-like sequence is also found in prokaryotes. These findings suggest that there are two cobalt-dependent MetAP families, presently composed of the prokaryote and yeast sequences (and represented by the E. coli structure) (type I), on the one hand, and by human MetAP, the yeast open reading frame, and the partial prokaryotic sequence (type II), on the other.

Two classes of metric spaces

The class of metric spaces (X,d) known as small-determined spaces, introduced by Garrido and Jaramillo, are properly defined by means of some type of real-valued Lipschitz functions on X. On the other hand, B-simple metric spaces introduced by Hejcman are defined in terms of some kind of bornologies of bounded subsets of X. In this note we present a common framework where both classes of metric spaces can be studied which allows us to see not only the relationships between them but also to obtain new internal characterizations of these metric properties.

Composición de la dieta y estrategia alimentaria de cinco especies de peces bentónicos de la costa de Cullera (España)

This paper provides data on diet and feeding habits of five benthic fish species (Torpedo torpedo (Linnaeus,1758), Mullus surmuletus (Linnaeus, 1758), Uranoscopus scaber (Linnaeus,1758), Scorpaena scrofa (Linnaeus,1758) and Synaptura lusitanica (Capello,1868)) common in the artisanal fisheries in the Cullera coast (Mediterranean sea – Spain) and T.torpedo, U.scaber y S. lusitánica feeding habits are almost unknown. T. torpedo preferred small preys like fishes, polychaetes and molluscs, these preys were feed in small portions. M. surmuletus showed the highest feeding dynamic, consuming small prey in large numbers like crustaceans (brachyura and amphypoda). U. scaber had similar feeding habits, but the numbers of preys in the stomach were lower. The principal preys were fishes, crustaceans, molluscs and polychaetes. S.scrofa ate larger prey items such as fish, followed by crustaceans and molluscs. Finally S. lusitánica had a high vacuity index, feeding polychaetes as the most important prey in their diet. Feeding strategy indicates a specialization of T.torpedo, S.scrofa and S. lusitanica; conversely M. surmuletus and U. scaber were generalized species.

Using WordNet relations and semantic classes in information retrieval tasks

In this paper we explore the use of semantic classes in an existing information retrieval system in order to improve its results. Thus, we use two different ontologies of semantic classes (WordNet domain and Basic Level Concepts) in order to re-rank the retrieved documents and obtain better recall and precision. Finally, we implement a new method for weighting the expanded terms taking into account the weights of the original query terms and their relations in WordNet with respect to the new ones (which have demonstrated to improve the results). The evaluation of these approaches was carried out in the CLEF Robust-WSD Task, obtaining an improvement of 1.8% in GMAP for the semantic classes approach and 10% in MAP employing the WordNet term weighting approach.

Ledger for the Classes of 1650-1663, 1649-1660

The leather-bound volume contains accounts for the Classes of 1650-1663 and Fellows. Many of the student bills were settled with payment in kind, often in the form of grain, apples, or livestock. The Steward's accounts with the College from 1656-1659 were entered on pages 295-303 and listed as "The steward is Creditor" and "The steward is Debitor." Credit entries include food, fuel, corn, and wages; debit entries list purchases and often identify the sellers.

Ledger for the Classes of 1703-1718, 1703-1731

The parchment-bound hardcover folio volume contains accounts for Fellows, Tutors, the President, and members of the Classes of 1703-1718. Payments were often made with bills of credit, money, and notes. The Steward's accounts with the College are interspersed in the volume.