Mapeamento físico dos genes ribosomais 18S e 5S em peixes do gênero Trichomycterus (Teleostei, Trichomycteridae)

The present study aimed to cytogenetic analysis and structural and molecular level of four fish species of the genus Trichomycterus: T. diabolus, T. iheringi, T. zonatus and T. cf. mimonha collected in different river basins in Brazil. Techniques were used for classical cytogenetic (Giemsa, Silver nitrate impregnation, C-banding) and molecular with the chromosomal location of genes for 18S and 5S rDNA. All individuals examined had a diploid number 54 chromosomes and karyotype consisting of types of metacentric, submetacentric and subtelocentric. The constitutive heterochromatin identified by C-banding was observed in two small blocks in the karyotype of T. diabolus and large blocks of centromeric several pairs in chromosomal karyotypes of T. iheringi, T. zonatus and T. cf. mimonha. The Silver nitrate impregnation and hybridization with 18S rDNA probe revealed the existence of only a couple carryng nucleolar organizing regions (NORs) on the species T. diabolus, T. iheringi and T. cf mimonha, and two pairs carrying 18S rDNA in T. zonatus. The 5S rDNA was observed in interstitial position 6 of the pair in T. iheringi, the synteny with the two pair in 18S rDNA of T.diabolus in pericentromeric position of and two pairs submetacentric, one being also a case of synteny with the 18S rDNA in T. zonatus and T. cf. mimonha, this rDNA was located in the pairs 3, 18 and 25, and synteny in the 18S rDNA pair 18. Although representatives of these four species Trichomycterus present diploid number and karyotypic formula preserved, three is specific about the distribuition patterns of heterochromatin and location of rDNA sequences, indicating that chromosomal differentiation events in this group of fish are acting directly on these genomic portions

A percepção do preparador físico sobre o estresse no futebol: um estudo piloto

Studies on stress in the sport are broad and profound. However, we do not found approach to study the perceptions of trainers on the subject and how it influences the organization of training throughout the season. From this premise, the aim of this work is to verify that the perception of the trainer about stress and its influence on planning. To this end, we chose a qualitative research, through an open interview, carried out with a trainer for a professional soccer team as a pilot for future studies. The interview responses are consistent with the findings of the literature on the changes in the players arising from the interference of stress in the various dimensions. But it was also possible to identify the trainer interviewed pointed out that stress, especially during the competition changes the preliminary planning, therefore, modify the activities to be applied to players depending on the outcome of matches. This pilot study we subsidized the development of new questions that will be inserted in the continuity of the study

Smart Grid e Carros Elétricos - Influência da Carga de Carros Elétricos Sobre o Sistema Elétrico

The technologies are advancing at a pace so expressive that allow the increase of the power quality from generation until the distribution to end customers. This improvement has been made possible through the automation of the energy that follows to a better quality of the energy provided, a lower energy supply disruptions and a very short recovery time. The trend of today and the near future is the distributed energy generation. To keep the automated control of the chain, the presence of Smart Grids is needed and that will be the most efficient and economical way to manage the entire system. Within this theme, is going to be necessary analyze the electric cars that promise to promote a more sustainable transport because it doesn’t uses fossil fuels, and more healthy because it does not emit pollutants into the atmosphere. The popularization of this type of vehicle is estimated to happen in a few decades and the case study analyzing its influence on the demand of the electrical system is something that will be very important in the near future. This paper presents a study of the influence of the inclusion of charges refering to electric cars

Produção e caracterização físico-mecânica do compósito cimento-madeira com variação granulométrica dos resíduos lignocelulósicos

The need to reduce environmental damage and add value to waste causes more and more new alternatives appear to unite these two points. One of the main ways to achieve this in timber industries and the use of waste for making panels. This work was aimed at studying the influence of particle size and density in Eucalyptus mechanical compressive strength of cement composite wood. For this study was performed production and physico-mechanical characterization of specimens, using portland cement, water and waste eucalyptus. The methodology consists of a statistical study of the results obtained by calculating the density and axial compression tests and a subsequent comparison of these results with other studies. The results showed that there are significant differences in density and compressive strength when using different particle sizes the particles of eucalyptus. In general, the smaller the particle size, the lower the compression strength and the greater the density when the samples are produced with the same trait

Análise dos efeitos das deformações estruturais sobre a transferência transversal de carga em um veículo do tipo fórmula SAE submetido a uma aceleração lateral constante

This paper studies the frame deformations on a formula SAE vehicle in steady-state cornering and its influence on the lateral load transfers and, consequently, on the tires normal loads due to the applied lateral load. For a vehicle with a perfect rigid frame, the vehicle mass, the position of the center of gravity and the suspensions are the only factors responsible for the load distribution between the tires. When the frame deformations are no longer negligible, the frame deformations affect the loaddistribution between the tires. The frame flexibility turns it able to behave as an additional set of springs to the suspension system, thus changing the behavior of the set. This paper describes howit happens and suggests ways to minimize this phenomenon

Caracterização físico-mecânica de elementos estruturais roliços perfilados de Amaru

The study of physical and mechanical properties of wood is essential for its structural use and it is of great importance to the construction industry. Thus, this study aimed to determine the physical and mechanical properties of the wood Amaru - a hybrid of Eucalyptus, developed by Plantar Projects and Forest Products Ltda. In order to determine the properties of Amaru, round samples were used, which were provided to the Laboratory of Wood and Wooden Structures of the School of Engineering of São Carlos, University of São Paulo - LaMEM / EESC / USP. For the characterization of the physical properties, the apparent specific gravity and moisture content of the samples were determined. To the mechanical characterization, the following properties were evaluated: strength and stiffness in compression, strength and stiffness in bending, shear and tension. The procedures of the tests performed in this study were done according to the recommendations of the Brazilian Wood Standard ABNT NBR 7190:1997. The specimen used were confectioned in actual dimensions, according to as those used in the construction system proposed by Plantar. The results obtained from the tests performed showed that the mechanical properties approached the values proposed by the Wood Standard NBR 7190. The visual grading was important to provide a primary idea about the failure modes to be obtained from the tests performed. The bending test showed the modulus of elasticity (MOE) and Modulus of Rupture (MOR), which resulted in 15822 MPa and 101,7 MPa, respectively. The compression test resulted in values Ec0,m and fc0, 15698 MPa and 50,7 MPa. The tensile strength (ft0) of this hybrid was calculated and its value obtained was 60,8 MPa. The shear strength (fv0) was 8,2 MPa. The results obtained from the tests are the basis for engineers and architects to design structures using wood species Amaru

Nitrogênio e fósforo na biomassa de Pistia stratiotes Linnaeus em dois ecossistemas lóticos com diferentes níveis de poluição orgânica da bacia do rio Itanhaém, SP

O objetivo desse trabalho foi avaliar as possíveis diferenças no teor de nitrogênio e fósforo na biomassa de Pistia stratiotes, que coloniza um rio com caracterísicas naturais (Aguapeú) e outro, que recebe grande carga de efluentes domésticos (rio do Poço). Para tanto, foram coletadas amostras de água e indivíduos de P.stratiotes (cinco réplicas) nesses rios. Paralelamente foram feitas medidas diretas de variáveis físico-químicas na água (pH, temperatura, condutividade elétrica, salinidade e turbidez). Em laboratório, foram determinados os teores de oxigênio dissolvido e a alcalinidade das amostras de água, bem como as concentrações de nitrogênio orgânico total, nitrogênio orgânico dissolvido, N-amoniacal N-nitrito, N-nitrato, fósforo total, fósforo dissolvido e P-ortofosfato. Os indivíduos de P. stratiotes coletados tiveram o número de folhas, diâmetro da roseta, altura da parte aérea e, o comprimento e volume da raiz medidos logo após a coleta. Esse material vegetal foi separado em biomassa aérea e submersa, seco e moído para a determinação da biomassa e dos teores de nitrogênio e fósforo totais. Observou-se que o rio Poço apresentou anoxia e as maiores concentrações de nutrientes na água. Pistia stratiotes apresentou maior biomassa e teores de nitrogênio e fósforo totais no rio Poço, provavelmente, devido às maiores concentrações de nutrientes deste rio. Por outro lado, a anoxia não foi limitante para o crescimento da espécie nesse rio. A maior proliferação de P stratiotes se deu no rio Poço, em relação ao rio Aguapeú, devido às condições de eutrofização em que se encontra esse ambiente, ocasionada pelo lançamento freqüente de efluentes domésticos, ricos em nutrientes, em seu curso d’água.

Estudo, desenvolvimento e concepção de uma célula de carga

In engineering projects, it’s fundamental to determine the active loads in components in order to guarantee acceptable values of safety and reliability according to project specifications. On the other hand, force measurement methods might be very complex and impracticable in some cases and, so that, load cells with eletric resistance strain gages can be applied as a simple and accurate option to measure the required load. The main purpose of this paper is to present the development of a load cell that measures uniaxial forces using electric resistance strain gages without being influenced by the location of the loading in a cantilever beam. For that, it was taken as basis a secondary purpose which is to present a general study of basic and wide concepts about transducers, load cells and extensometers primarily. Information such as: loading and measurements types, characteristics of the presented devices as well as factors that influence its functioning, the most common kinds of Wheatstone bridge links, the main points of a load cell project, cements used to fix extensometers and, finally, the project itself with the tests of the built transducer are presented. By the end of this paper, all the results are shown and analyzed, concluding about the designed load cell and the work itself

Análise do desempenho físico-mecânico de painéis compensados de Pinus sp. produzidos com adesivos aquosos a base de poliacetato de vinila e de homopolímero vinílico

This study aimed to evaluate the physico-mechanical performance of three PVA adhesive formulations according to the Brazilian standards for plywood testing and to evaluate the results with those presented in the technical literature and specified in normative documents. The study presents the production process of the panels, the obtaining of samples and, finally, the implementation of physico-mechanical characterization tests based on plywood Brazilian standards. For the panels production it was used a gramature of 120 g / m² and applied a pressure of 15 bars and two temperature combinations and pressing time for each adhesive. The commercial adhesives used were PVA 1810, PVA 1060 and Vinyltec 239. The physical tests conducted were thickness swelling, density, moisture content and water absorption. The mechanical tests were static flexion, for the determination of elasticity modulus (MOE) and rupture modulus (MOR), and shear in the glue line. Among the three formulations studied none showed good performance in the presence of water. However, other trials in the adhesive showed better results and closer to the commercially equivalent used, in the case the urea-formaldehyde, was the PVA 1810, that can be applied in non-structural uses and in environments without the presence of moisture

Aspectos clínicos e cirúrgicos do osteossarcoma canino

Osteosarcoma is the primary osseous neoplasia frequently diagnosed in dogs and it’s the main answerable for more than 85% of the neoplasias origened from the skeleton. The average age of onset of the lesion is 7 years, especially in large breeds and giant, with males being more affected than females. In general, 75% of osteosarcomas develop in the appendicular skeleton and 25% in the axial. The forelimbs are more affected than the hindlimbs, since support 60% of body weight. The osteosarcoma diagnosis is made by the analys of the animal’s clinic historic, detailed physique exams, blood exams alkaline phosphatase, xray exams, cytology, biopsy. The treatment of choice is the amputation associated to the chemotherapy. However, in some cases the member is preserved by the allograft technic. The chemotherapy associated to the surgery reduces the total load of the tumor, lengthens the interval of the life and improves the quality of pacient’s life. The most used medicines are cisplatin and doxorubicin. They can be used in separated or associated ways. The prognostic of dogs with osteosarcoma is reserved

Efeitos da interação entre o uso de doses suprafarmacológicas de decanoato de nandrolona e do exercício físico sobre a próstata de ratos adultos

Os hormônios esteróides anabólico-androgênicos (EAA) compreendem a testosterona e seus derivados. Atletas e não atletas interessados em aumentar o desempenho esportivo, a força, a aparência física e a massa muscular têm utilizado esteróides anabólico-androgênicos sintéticos (EAAs) sem indicação médica. O uso de EAAs pode causar câncer de próstata, doença coronariana e esterilidade. No presente trabalho, avaliou-se os efeitos de altas doses de decanoato de nandrolona, do exercício físico resistido e da interação desses fatores sobre os lobos prostáticos de ratos machos adultos. Vinte ratos foram divididos aleatoriamente em quatro grupos experimentais e tratados e pesados por oito semanas: Esteróide + Exercício (EE), Sedentário + Esteróide (ES), Veículo + Exercício (VE) e Veículo + Sedentário (VS). Eles receberam injeção i.m de decanoato de nandrolona (5mg/Kg) e veículo (propilenoglicol) (0,2 mL/Kg). O peso absoluto corpóreo e do lobo ventral da próstata foi aferido. Os lobos prostáticos foram coletados, dissecados e processados, empregando-se técnicas microscópicas (histológicas, histoquímicas e imunohistoquímicas) e morfométricas. Os dados foram analisados por ANOVA bifatorial e Tukey (p<0,05). Alterações estatisticamente significativas nas massas corpórea, do lobo ventral, no índice de proliferação celular, na população de células basais e na análise dos volumes relativos dos componentes prostáticos (epitélio, estroma e lúmen) foram observadas. O decanoato de nandrolona determinou o aumento da proliferação celular, sugerindo o desenvolvimento da hiperplasia hormonal. De modo geral, as respostas não foram homogêneas para os parâmetros analisados, visto que os tecidos apresentam níveis de expressão de receptores de andrógenos, afinidade de ligação e vias metabólicas de conversão... (Resumo completo, clicar acesso eletrônico abaixo)

Simulação de contaminação de sedimentos e águas subterrâneas por gasolina e álcool: um estudo de monitoramento de variações das propriedades físico-químicas

Geophysics studies in areas impacted by petroleum derivatives describe abnormalities of both low and high electrical resistivity (the opposite of electrical conductivity), confirmed as contaminant phase by chemical analysis: this contradiction can be explained by degrading processes that naturally occur and create sub products that can change the environment conductivity. Monitoring the variation of the parameters mentioned serves as a comparative basis to the variation in geoelectrical parameters, which identified the correlation between the same contaminant parameters and the difference between their behavior studied apart, as well as its relations with the biodegradation process. The results are applied to the fuel distribution and storage sectors, leading to the diagnosis and monitoring of possible groundwater contamination scenarios, and the knowledge of the area exposure time to the contaminant, besides the better remediation alternative and impacts control. Among some conclusions, the most significant are the decrease in conductivity over time, so as the increase in Eh value in the gasoline contaminated tank, as well as the decrease in the pH value in the second tank with ethanol, which can be attributed to its degradation. Comparing the variations in both tanks, it is evident that Eh, pH and electrical conductivity do not behave temporally in a similar way, although some correlations between Eh and pH can be related.

Revisão: propriedades físico-químicas do biodiesel

The present literature review aimed to describe biodisel’s physicochemical properties obtained from different raw materials. Were studied data concerning viscosity, density, cetane number, fl ash point, pour point and calorifi c power of biodiesel produced from soybean oil, coconut, rice bran, cotton, pequi, babassu, mamona, palm, castor, sunfl ower, corn, canola, jatropha and karanja. Considering the diversity of vegetal and animal sources that can be used on the biodiesel production, it is noteworthy the lack of data concerning physicochemical properties of unexplored raw materials. This work may contribute for the creation of database about physicochemical properties of oil and biodiesel from different sources which will allow design and scale-up, both the necessary equipment to the production line and reciprocating engines.

Avaliação da influência do formato da porca e da pré-carga em juntas aparafusadas em tração

Many structures and mechanical equipment requires a union member, and the screw is one of the most used, the cost is low, it is versatile and easy to find on the market. The resulting pre-load assembly torque associated with the cyclical external load has great influence, so it will be studied carefully. This study analyzed some of these factors, as the format of nuts, and the washer material when they are incompatible with the bolt material can cause a reduction in life of the joint fatigue

Sistema de gerenciamento de energia para controle de carga/descarga entre baterias de lítio e ultracapacitores, envolvendo sistema de tração elétrica com frenagem regenerativa

Pós-graduação em Engenharia Elétrica - FEIS