Developing Hera-FFX for WCAG 2.0

WCAG 2.0 was published in December 2008. It has many differences to WCAG 1.0 as to rationale, structure and content. Two years later there are still few tools supporting WCAG 2.0, and none of them fully mirrors the WCAG 2.0 approach organized around principles, guidelines, success criteria, situations and techniques. This paper describes the on-going development of an update to the Hera-FFX Firefox extension to support WCAG 2.0. The description is focused on the challenges that we have found and our resulting decisions.

A substance P (neurokinin-1) receptor mutant carboxyl-terminally truncated to resemble a naturally occurring receptor isoform displays enhanced responsiveness and resistance to desensitization

Two isoforms of the substance P (SP) receptor, differing in the length of the cytoplasmic carboxyl-terminus by ≈8 kDa, have been detected previously in rat salivary glands and other tissues. The binding and functional properties of these two isoforms have been investigated using full-length (407 amino acids) and carboxyl-terminally truncated (324 amino acids) rat SP receptors transfected stably into Chinese hamster ovary cells. Both the full-length and the truncated receptor bound radiolabeled SP with a similar Kd (≈0.1 nM). The average number of high affinity SP binding sites per cell was 1.0 × 105 and 0.3 × 105 for the full-length and the truncated SP receptor, respectively. In both cell lines, SP induced a rapid but transient increase in cytosolic calcium concentration ([Ca2+]i), which consisted of the release of Ca2+ from intracellular stores and the influx of extracellular Ca2+. Both components are dependent on phospholipase C activation. Although the full-length and the truncated receptor utilize the same calcium pathways, they differ in their EC50 values (0.28 nM for the full-length; 0.07 nM for the truncated). These differences in responsiveness may be related to the observed differences in receptor desensitization. The truncated receptor, in contrast to the full-length receptor, does not undergo rapid and long-lasting desensitization. Cells possessing the short isoform of the SP receptor would thus be expected to exhibit a prolonged responsiveness.

Major histocompatibility complex class II DR alleles DRB1*1501 and those encoding HLA-DR13 are preferentially associated with a diminution in maternally transmitted human immunodeficiency virus 1 infection in different ethnic groups: determination by an automated sequence-based typing method.

Transmission of human immunodeficiency virus 1 (HIV-1) from an infected women to her offspring during gestation and delivery was found to be influenced by the infant's major histocompatibility complex class II DRB1 alleles. Forty-six HIV-infected infants and 63 seroreverting infants, born with passively acquired anti-HIV antibodies but not becoming detectably infected, were typed by an automated nucleotide-sequence-based technique that uses low-resolution PCR to select either the simpler Taq or the more demanding T7 sequencing chemistry. One or more DR13 alleles, including DRB1*1301, 1302, and 1303, were found in 31.7% of seroreverting infants and 15.2% of those becoming HIV-infected [OR (odds ratio) = 2.6 (95% confidence interval 1.0-6.8); P = 0.048]. This association was influenced by ethnicity, being seen more strongly among the 80 Black and Hispanic children [OR = 4.3 (1.2-16.4); P = 0.023], with the most pronounced effect among Black infants where 7 of 24 seroreverters inherited these alleles with none among 12 HIV-infected infants (Haldane OR = 12.3; P = 0.037). The previously recognized association of DR13 alleles with some situations of long-term nonprogression of HIV suggests that similar mechanisms may regulate both the occurrence of infection and disease progression after infection. Upon examining for residual associations, only only the DR2 allele DRB1*1501 was associated with seroreversion in Caucasoid infants (OR = 24; P = 0.004). Among Caucasoids the DRB1*03011 allele was positively associated with the occurrence of HIV infection (P = 0.03).

Identification of endothelin 1 and big endothelin 1 in secretory vesicles isolated from bovine aortic endothelial cells.

Vesicles containing endothelin 1 (ET-1) were isolated from bovine aortic endothelial cells (BAECs) by fractionation of homogenates on sucrose density gradients by ultracentrifugation. The vesicles were localized at the 1.0/1.2 M sucrose interface using a specific anti-ET-1-(16-21) RIA. Identification of ET-1 and big ET-1 in this fraction was confirmed by HPLC analysis combined with RIA. Morphological examination of the ET-1-enriched fraction by electron microscopy identified clusters of vesicles approximately 100 nm in diameter. Immunostaining of ultrathin cryosections prepared from the vesicle fraction for ET-1 or big ET-1 showed clusters of 15-nm gold particles attached to or within vesicles. Immunofluorescence staining of whole BAECs using a specific ET-1-(16-21) IgG purified by affinity chromatography revealed punctate granulation of the cell cytoplasm viewed under light microscopy. This distinct pattern of staining was shown by confocal light microscopy to be intracellular. Immunofluorescence staining of whole cells with a polyclonal antiserum for big ET-1-(22-39) showed a defined perinuclear localization of precursor molecule. Hence, several different approaches have demonstrated that ET-1 and big ET-1 are localized within intracellular vesicles in BAECs, suggesting that these subcellular compartments are an important site for processing of big ET-1 by endothelin-converting enzyme.

Análise da expressão da proteína NY-ESO-1 no melanoma cutâneo

INTRODUÇÃO: o câncer é a doença que mais mata pessoas com idade abaixo de 85 anos e é um problema de saúde pública. Os tumores podem expressar em determinada fase de seu desenvolvimento proteínas anômalas que podem ser alvo de métodos diagnósticos e de intervenções terapêuticas. A expressão de NY-ESO-1 é detectada em 20 a 40% dos melanomas. Há evidências que esta expressão é mais freqüente em tumores de estágios mais avançados e está associada a um pior prognóstico. OBJETIVOS: determinar a frequência de expressão da proteína NY-ESO-1 no melanoma cutâneo e tentar correlacioná-la com o índice de Breslow, aspectos histopatológicos do melanoma, incluindo o infiltrado linfocítico tumoral, e a morbi-mortalidade dos pacientes. MÉTODOS: o presente estudo é longitudinal de coorte retrospectiva e foi realizado de agosto de 2009 a outubro de 2015. Foram selecionados 89 melanomas de 87 pacientes do Ambulatório de Tumores do Departamento de Dermatologia da FMUSP, divididos em 3 grupos, sendo: grupo 1: 34 melanomas com índice de Breslow <= 1,0 mm; grupo 2: 29 melanomas com índice de Breslow entre 1,1 - 4,0 mm e grupo 3: 26 melanomas com índice de Breslow >= 4,0 mm. As lâminas dos exames anátomo-patológicos destes pacientes foram revisadas quanto ao diagnóstico de melanoma, seu índice de Breslow e a presença de infiltrado linfocítico tumoral. A seguir, realizou-se exame de imunohistoquímica para a determinação da presença do antígeno NY-ESO-1 em todos os 89 tumores coletados e em mais 20 nevos (11 displásicos e 9 intradérmicos) escolhidos ao acaso. Através da revisão dos dados do prontuário, foram obtidos os dados clínicos de: idade, sexo, raça, fototipo da pele, local de aparecimento do melanoma, status do linfonodo sentinela quando realizado, desenvolvimento de metástases e sobrevida dos pacientes. Os dados anátomo-patológicos do tumor analisados foram: tipo histológico, presença de ulceração, e tipo de infiltrado linfocítico tumoral. Nos melanomas que apresentavam infiltrado linfocítico tumoral, foram realizados testes imunohistoquímicos para pesquisa de células CD3+, CD8+, FoxP3+ e CD8+FoxP3+ (duplamente positivas). RESULTADOS: O antígeno NY-ESO-1 esteve presente em 19% dos melanomas cutâneos primários e não foi detectado em nenhum dos 20 nevos pesquisados. A expressão do antígeno NY-ESO-1 esteve estatisticamente relacionada a tumores com espessuras maiores. Apresentou também uma associação inversa com o tipo extensivo superficial em relação aos outros tipos histológicos. O infiltrado linfocítico tumoral dos melanomas NY-ESO-1 positivos continha menor número de células CD3+, que se encontravam isoladas ou arranjadas em pequenos grupos de até 5 células, o que contrastava significantemente com os tumores NY-ESO-1 negativos, com maior densidade de células CD3+, dispostas em grandes grupos, com 6 ou mais células. A expressão da proteína NY-ESO-1 não esteve associada à idade, ao sexo, ao fototipo, ao sítio primário do tumor, à presença de ulceração, ao status do linfonodo sentinela, ao desenvolvimento de metástases ou à sobrevida. CONCLUSÕES: Há expressão de NY-ESO-1 em uma porcentagem considerável dos melanomas, principalmente nos mais espessos. O menor número de células CD3+ no infiltrado linfocítico tumoral, acrescido ao fato destas células estarem isoladas ou em pequenos grupos, sugere que embora imunogênico, a expressão do antígeno NY-ESO-1 não resulta num estímulo eficaz do sistema imune no combate ao tumor. O desenvolvimento de uma vacina para estes pacientes poderá, no futuro, aumentar as possibilidades terapêuticas do melanoma

China 1:200,000 : Sheet H-50-XXIV : Zhejiang Sheng Region (Raster Image)

This layer is a georeferenced raster image of the Soviet Army topographic sheet map covering a portion of the Zhejiang Sheng region, China (map quadrangle number: H-50-XXIV). It is from a series of Soviet Army topographic maps of China 1:200,000. The source map was published in 1979. The image inside the map neatline is georeferenced to the surface of the earth and fit to the Pulkovo 1942 GK Zone 20N projection. Map collar information from the source map have been cropped and are not available as part of the raster image. China 1:200,000 topographic maps were prepared and printed by the Soviet Army General Headquarters, 1976-1991. China 1:200,000 maps are in Russian. Each source map in the series is printed in color. China 1:200,000 maps are typical topographic maps portraying both natural and manmade features. They show and name works of nature, such as mountains, valleys, lakes, rivers, vegetation, etc. They also identify the principal works and structures of humans, such as roads, railroads, paths, walls, boundaries, transmission lines, major buildings, etc. Relief is shown with standard contour intervals of 40 meters and/or spot heights.

China 1:200,000 : Sheet H-50-XXX : Zhejiang Sheng Region (Raster Image)

This layer is a georeferenced raster image of the Soviet Army topographic sheet map covering a portion of the Zhejiang Sheng region, China (map quadrangle number: H-50-XXX). It is from a series of Soviet Army topographic maps of China 1:200,000. Published in 1979, the source map was compiled from maps 1:100,000 published in 1977; corrected according to source material, 1970-1975. The image inside the map neatline is georeferenced to the surface of the earth and fit to the Pulkovo 1942 GK Zone 20N projection. Map collar information from the source map have been cropped and are not available as part of the raster image. China 1:200,000 topographic maps were prepared and printed by the Soviet Army General Headquarters, 1976-1991. China 1:200,000 maps are in Russian. Each source map in the series is printed in color. China 1:200,000 maps are typical topographic maps portraying both natural and manmade features. They show and name works of nature, such as mountains, valleys, lakes, rivers, vegetation, etc. They also identify the principal works and structures of humans, such as roads, railroads, paths, walls, boundaries, transmission lines, major buildings, etc. Relief is shown with standard contour intervals of 40 meters and/or spot heights.

China 1:200,000 : Shaoxing Region, Zhejiang Sheng (Raster Image)

This layer is a georeferenced raster image of the Soviet Army topographic sheet map of the Shaoxing region, Zhejiang Sheng, China (map quadrangle number: H-51-XIX). It is from a series of Soviet Army topographic maps of China 1:200,000. Published in 1979, the source map was compiled from maps 1:100,000 published in 1977; corrected according to source material, 1973, 1974. The image inside the map neatline is georeferenced to the surface of the earth and fit to the Pulkovo 1942 GK Zone 20N projection. Map collar information from the source map have been cropped and are not available as part of the raster image. China 1:200,000 topographic maps were prepared and printed by the Soviet Army General Headquarters, 1976-1991. China 1:200,000 maps are in Russian. Each source map in the series is printed in color. China 1:200,000 maps are typical topographic maps portraying both natural and manmade features. They show and name works of nature, such as mountains, valleys, lakes, rivers, vegetation, etc. They also identify the principal works and structures of humans, such as roads, railroads, paths, walls, boundaries, transmission lines, major buildings, etc. Relief is shown with standard contour intervals of 40 meters and/or spot heights.

China 1:200,000 : Xianju Region, Zhejiang Sheng (Raster Image)

This layer is a georeferenced raster image of the Soviet Army topographic sheet map of the Xianju region, Zhejiang Sheng, China (map quadrangle number: H-51-XXV). It is from a series of Soviet Army topographic maps of China 1:200,000. Published in 1986, this map reflects 1979 ground conditions. The source map was compiled from maps 1:100,000 published in 1979. The image inside the map neatline is georeferenced to the surface of the earth and fit to the Pulkovo 1942 GK Zone 20N projection. Map collar information from the source map have been cropped and are not available as part of the raster image. China 1:200,000 topographic maps were prepared and printed by the Soviet Army General Headquarters, 1976-1991. China 1:200,000 maps are in Russian. Each source map in the series is printed in color. China 1:200,000 maps are typical topographic maps portraying both natural and manmade features. They show and name works of nature, such as mountains, valleys, lakes, rivers, vegetation, etc. They also identify the principal works and structures of humans, such as roads, railroads, paths, walls, boundaries, transmission lines, major buildings, etc. Relief is shown with standard contour intervals of 40 meters and/or spot heights.

China 1:200,000 : Wezhou Region, Zhejiang Sheng (Raster Image)

This layer is a georeferenced raster image of the Soviet Army topographic sheet map of the Wenzhou region, Zhejiang Sheng, China (map quadrangle number: H-51-XXXI). It is from a series of Soviet Army topographic maps of China 1:200,000. Published in 1986, this map reflects 1979 ground conditions. The source map was compiled from maps 1:100,000 published in 1979. The image inside the map neatline is georeferenced to the surface of the earth and fit to the Pulkovo 1942 GK Zone 20N projection. Map collar information from the source map have been cropped and are not available as part of the raster image. China 1:200,000 topographic maps were prepared and printed by the Soviet Army General Headquarters, 1976-1991. China 1:200,000 maps are in Russian. Each source map in the series is printed in color. China 1:200,000 maps are typical topographic maps portraying both natural and manmade features. They show and name works of nature, such as mountains, valleys, lakes, rivers, vegetation, etc. They also identify the principal works and structures of humans, such as roads, railroads, paths, walls, boundaries, transmission lines, major buildings, etc. Relief is shown with standard contour intervals of 40 meters and/or spot heights.

Massachusetts, 1795 (Image 1 of 2) (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: An accurate map of the Commonwealth of Massachusetts exclusive of the District of Maine : compiled pursuant to an act of the General Court from actual surveys of the several towns &c. taken by their order, exhibiting the boundary lines of the Commonwealth, the counties and towns, the principal roads, rivers, mountains, mines, islands, rocks, shoals, channels, lakes, ponds, falls, mills, manufactures & public buildings, with the true latitudes & longitudes, &c., by Osgood Carleton. It was published and sold by O. Carleton and I. Norman in 1795. Scale [ca. 1:281,560]. This layer is image 1 of 2 total images, representing the eastern portion of the two sheet source map. The image inside the map neatline is georeferenced to the surface of the earth and fit to the Massachusetts State Plane Coordinate System, Mainland Zone (in Feet) (Fipszone 2001). All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, or other information associated with the principal map. This map shows features such as roads, bridges, academies, meeting houses, court houses, drainage, mountains, iron ore deposits, coastal navigational hazards, state, county, and town boundaries, distances of individual towns from Boston and the shire towns, and more. Relief is shown pictorially. Includes illustrative cartouche. This layer is part of a selection of digitally scanned and georeferenced historic maps of Massachusetts from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of regions, originators, ground condition dates (1755-1922), scales, and purposes. The digitized selection includes maps of: the state, Massachusetts counties, town surveys, coastal features, real property, parks, cemeteries, railroads, roads, public works projects, etc.