Efeito de temperaturas alternadas sobre desenvolvimento de Aedes (Stegomyia) aegyoti (L.) (Diptera: Culicidae)

Aedes (Stegomyia) aegypti is a species of mosquitoes known to be the vector of diseases such as dengue and yellow fever, and a better understanding of aspects of their biology can help in the establishment of control strategies for the same. Several previous studies showed that temperature significantly affects the development of immature stages of insects. In general, higher temperatures (up to a threshold) accelerate the development of insects, and lower temperature retards the same. This rule also applies to mosquitoes, including Ae. aegypti. But not still know the effects of daily variation of temperature on the developmental stages of mosquitoes. And this detail is very important, since in natural breeding or artificial, The mosquitoes usually face temperature variations over a single day, which should interfere with its development until the emergence of the adult forms. For this reason, the objective of this study is to analyze the effect of alternating temperatures on the development of Ae. aegypti. To conduct the study, adults were collected active in the neighborhood Bela Vista Campus of UNESP - Rio Claro, SP, using a sweep net or using ovitraps for immatures, and the active search for breeding. Individuals collected were kept under experimental conditions in the laboratory. The adult samples were identified to species level, were considered for the experiments, only samples of Ae. aegypti. The insects were housed in plastic cages, suitable for creating flies. These were fed with sugar solution and blood meal on alternate days. The eggs obtained were used in the experiment with four different temperature regimes. The data collected were analyzed by evaluating whether the different treatments influenced the development of immature to adult, performing the Kruskal-Wallis test and the statistical software BioEstat. Statistical analysis of the sex ratio... (Complete abstract click electronic access below)

Monitoramento das águas subterrâneas do aterro de resíduos da construção civil e inertes, no bairro Jd. Wenzel, em Rio Claro-SP

This work is proposed to evaluate the potential risk of an inert landfill in the city of Rio Claro (SP), and also suggest a source for the contamination. The study was proposed because it is known that there were legal technicalities greetings to guarantee the quality of groundwater. Given the requirements of the Environmental Company of São Paulo (CETESB) were installed monitoring wells in the area; sampled to groundwater and its contamination was found. In addition, we analyzed the main characteristics of the area, the arrangement and characteristics of the waste, the local geology and the hydrogeological system, so that I could find the source of contaminants, and predict the direction of dispersion even in groundwater. At the end of the work presents conclusions, recommendations and proposals for mitigating action

WSPControl: aplicação distribuída baseada em Web Services para monitoramento remoto de computadores

The tool proposed, known as WSPControl, enables remote monitoring of computers across the Internet using distributed applications. Through a Web Services architecture is possible the communication between these distributed applications across heterogeneous platforms, also eliminates the need for additional settings in computer networks, such as release of ports or proxy. The tool is divided into three modules, namely: • Client Interface: developed in C Sharp, is responsible for capturing data on performance of the monitored computer also connects to the Web Services to report this data. • Web Services Interface: developed in PHP using the PHP SOAP library, is responsible for facilitating the communication between internet applications and client. • Internet Interface: developed in PHP, is responsible for reading and interpreting the information captured these available on the Internet

Aplicação de técnicas de geoprocessamento para a avaliação da expansão urbana no municípo de Duque de Caxias (RJ) como subsídio ao monitoramento de dutos: trecho da faixa de dutos Rio de Janeiro - Belo Horizonte

Different forms of human pressure may occur in the pipeline ranges, due to the large extensions and various configurations of land use, which can pass through the pipelines. Due to the dynamics of these pressures, it is necessary to monitor temporal changes of land use and cover the surface. Under this theme, appears as extremely important to use products and techniques of remote sensing, as they allow the identification of objects of the land surface that may compromise the security and monitoring of the pipeline, and allows the extraction of information conditions on land use at different periods of time. Based on the above, this paper aims to examine in a temporal approach, the process of urban expansion in the municipality of Duque de Caxias, located on the outskirts of the metropolitan area of the state of Rio de Janeiro, as well as settlement patterns characteristic of areas that the changes occurred in the period 1987 to 2010. We used the technique of visual analysis to perform the change detection and the technique of image classification, aimed at monitoring human pressure over a stretch of track pipeline Rio de Janeiro - Belo Horizonte, located in the state of Rio de Janeiro. The stages of work involved the characterization of the study area, urban sprawl and the existing settlement patterns, through the analysis of bibliographic data. The processing of Landsat 5 images and the application of the technique of change detection were performed in three scenes for the years 1987, 1998 and 2010, while the classification process was performed on the image RapidEye for the year 2010. Can be noted an increase in urban area of approximately 22.38% and the change of land cover from natural to built. This growth is concentrated outside to the area of direct influence of the duct, occurring in the area of indirect influence of the enterprise. Regarding the settlement patterns of growth areas, it was observed that these are predominantly

Utilização de técnicas de geoprocessamento para monitoramento de atividades de mineração ao longo de dutos: poliduto Rio de Janeiro/Minas Gerais

The transportation of oil through polyducts implies a concern related to safety and environmental impacts they may cause, especially when exposed to risks that affect their integrity. Among the various anthropogenic activities included in this scenario, mining can aggravate, increase the risks and degrade the environment. Since these polyducts go through significant extensions, remote sensing has great applicability as a tool for data acquisition. For this, change detection techniques were used to monitor mining activities in a defined study area in the state of Rio de Janeiro, along the duct ORBEL. The characterization of the study area and the mining activities were developed through bibliographical data. The satellite images processing and the application of change detection technique were performed in two scenes for the years 2002 and 2010. The growth in the mining area was about 6.67 times and it was possible to identify types of extraction involved which can represent direct risks to the pipeline

Monitoramento hidrossedimentlógico da bacia hidrográfica do alto rio Sucuriú (MS) contribuinte da PCH Costa Rica

The growing demand for electricity in Brazil has stimulated the implementation of Small Hydro Power (PCH) in various regions of the country. However, the silting of reservoirs is a major problem faced by power plants and power plants. In this context, this study aimed to evaluate the data hydrosedimentological strategic points of the watershed of the Alto Rio Sucuriú (MS) to identify the possible causes siltation of the reservoir PCH Costa Rica and suggest mitigation measures. Hydrosedimentological surveys were conducted during the rainy season (February / March 2012) and drought (August 2012), and obtained data flow, discharge liquid, suspended solids and bottom and organic matter content. Based on these results it was determined that the points 2, 4, 7 and 9 are the largest contributors to sedimentation, and point 4 got most liquid discharge (38,20 m3s-1), point 7 largest discharge of solid suspension (906,3 mg L-1), points 2 and 4 major discharges solid totals (231,59 t dia-1 and 238,185 t dia-1 respectively) and point 9 higher organic matter content (22,18%) . Found greater fraction of fine sand and very fine suspended solids and solid medium sand in the background. As mitigation measures for the process of silting of the reservoir PCH Costa Rica (MS) highlights the orientation of landowners in adopting conservation measures and planting of leguminous species native to the region in symbiosis with nitrogen-fixing bacteria

Monitoramento espacial e análise das características climáticas na influência sobre a cultura de soja no estado do Paraná

In the past years, soya has increased itself as the mean agro export culture in Brazil, encouraging the expansion of its agricultural frontier throughout the country. Brazil is the second biggest soya producer around the world, with a 59,8 million ton production in 2008, according to the Instituto Brasileiro de Geografia e Estatística (IBGE), only behind United States. Around the country, the four leading producer states are Mato Grosso, Paraná, Rio Grande do Sul e Goiás. Therefore, geotechnologies may be used to monitor use and soil occupation in various analyzes periods. In this review, the tools are important to illustrate the soya production areas and also the weather behavior around its production evolution in the territory through the years. Its utilization can contribute to the evolution and optimization around real time monitoring of the Agricola cultures, without being necessary to be in the area with a low financial cost. Generally, this information is strongly important for decision makers in both government and private sector, as soon as the achievement information regards the quantification of area, yield and development of agricultural crops are essential to the economic behavior of culture during the season and even beyond. By obtaining data regarding climate crops 2008/09 and 2009/10, held the climatic water balance calculation based on the dynamics of water storage in soil temperature and precipitation data, interpolation of the data through the interpolator (IDW) that generated thematic precipitation maps. Overall, the use of geotechnology to monitor agricultural areas, can strongly contribute to this monitoring, generating raw material for further analysis at low cost

Simulação de contaminação de sedimentos e águas subterrâneas por gasolina e álcool: um estudo de monitoramento de variações das propriedades físico-químicas

Geophysics studies in areas impacted by petroleum derivatives describe abnormalities of both low and high electrical resistivity (the opposite of electrical conductivity), confirmed as contaminant phase by chemical analysis: this contradiction can be explained by degrading processes that naturally occur and create sub products that can change the environment conductivity. Monitoring the variation of the parameters mentioned serves as a comparative basis to the variation in geoelectrical parameters, which identified the correlation between the same contaminant parameters and the difference between their behavior studied apart, as well as its relations with the biodegradation process. The results are applied to the fuel distribution and storage sectors, leading to the diagnosis and monitoring of possible groundwater contamination scenarios, and the knowledge of the area exposure time to the contaminant, besides the better remediation alternative and impacts control. Among some conclusions, the most significant are the decrease in conductivity over time, so as the increase in Eh value in the gasoline contaminated tank, as well as the decrease in the pH value in the second tank with ethanol, which can be attributed to its degradation. Comparing the variations in both tanks, it is evident that Eh, pH and electrical conductivity do not behave temporally in a similar way, although some correlations between Eh and pH can be related.

Monitoramento da condição da ferramenta de dressagem usando sinais de vibração e modelos neurais

Pós-graduação em Engenharia Elétrica - FEB

Effect of caffeine on larval mortality of Aedes aegypti: efficiency related to solution concentration and age

In two experiments, the duration of the effect of caffeine (CAF) solutions on larval mortality (LM) of Aedes aegypti was analyzed. In the first, LM was studied using solutions at 0.2, 0.5, 1.0 and 2.0 mg/mL aged from zero to five days in artificial breeding sites exposed to the laboratory environment (LE). In the second, the solutions aged at 1.0, 2.0 and 2.5 mg/mL closed flasks were stored in LE or in the refrigerator (R), and the effect on LM was tested in the experimental breeding sites at 30 days interval. In the first, the duration of the effect increased with the solution age in each CAF concentration. CAF at 1.0 and 2.0 mg/mL, without addition of food, produced 100% LM until 25 days after preparation; with food, at 11 and 18 days, respectively. In the second the effectiveness of CAF solutions lasted up to the seventh month, irrespective of whether they were stored in R or in LE. No adult emerged at any of the CAF concentrations used in second experiment.

Analise microbiológica da flora intestinal do mosquito Aedes Aegypti através do sequenciamento do gene 16S rRNA na plataforma Illumina MiSeq

Dengue é uma arbovirose que afeta cerca de 100 milhões de pessoas anualmente, em mais de 100 países situados nas regiões tropicais e subtropicais. Foi considerada a doença viral que mais cresceu no ultimo ano, repercutindo em impactos sociais e econômicos nas regiões endêmicas devido às altas taxas de morbidade e mortalidade desencadeadas pela infecção. O principal vetor da dengue é o mosquito Aedes aegypti, presente em toda a faixa tropical e subtropical. Por apresentar hematofagia antropofílica, rápido desenvolvimento e características comportamentais especificas, é um excelente transmissor do vírus dengue. Medidas de controle da disseminação da dengue são restritas à eliminação do mosquito vetor, e um tratamento específico ainda não foi desenvolvido, bem como a criação de uma vacina que previna simultaneamente a infecção pelos quatro sorotipos do arbovírus. Uma característica que determina a disseminação de doenças é a alta competência vetorial de seus mosquitos transmissores, que tem sido associada à composição da microbiota intestinal do inseto. As bactérias presente no intestino do mosquito exercem funções relacionadas a sua nutrição, desenvolvimento e reprodução, e são também um importante fator na eliminação de patógenos, por interferirem diretamente na atividade viral, ou indiretamente a partir da ativação das vias antivirais pelos micro-organismos. Dessa forma, este trabalho visa estudar a diversidade microbiana intestinal do mosquito Aedes aegypti em diferentes estágios de vida, através de sequenciamento de última geração com a plataforma MiSeq Illumina

Estudo para correção dos sensores da rede de monitoramento do campo elétrico em função do campo elétrico de tempo bom

A monitoring network of atmospheric electric field covering the Vale do Paraiba region was implemented. The sensors were located on different sites with different altitude and geographic topology. The present work reports the study carried on those sensors in order to verify the necessity of using some correction factor to the measured local electric field intensity due to effects of local environment. The measurements were done in continuous 24 hours per day with the data recorded on registers in each device accumulating information during a period of four months. The relation between the electric field values by each sensor was compared to the reference located on Sao Jose dos Campos city using the same period. In a graphical analysis using the local field intensity and the reference, the data were fitted to a straight line obtained by minimum square method. Variation up to 95% was observed between the field values in some sensors. Another method was proposed, comparing the mean values of the electric field in a function of time. The variation in some sensors reached up to 133%. We conclude that the variations are due to local atmospheric conditions and no correction factor is required on the electric field sensors

Monitoramento do perfil microbiológico de dentes com infecção endodôntica primária durante terapia com diferentes medicações intracanal

Pós-graduação em Odontologia Restauradora - ICT

Avaliação dos componentes de óleos essenciais 1,8-cineol, β-cariofileno e α-humuleno como possíveis repelentes para Aedes (Stegomyia) aegypti (Diptera: Culicidae)

Aedes aegypti, mosquito da família Culicidae responsável pela transmissão dos vírus da dengue, da febre amarela urbana e da febre chikungunya, apresenta grande proliferação em áreas urbanas das regiões tropicais e subtropicais, além de representar um fator de incômodo por causar alergias devido à inoculação dos componentes salivares durante o processo do repasto sanguíneo. Como forma de evitar os problemas causados por eles, o repelente mais usado e registrado comercialmente desde 1957 é o DEET (N,N-dietil-3-metilbenzamida ou N,N-dietil-meta-toluamida), que apesar de apresentar eficácia comprovada possui determinada toxicidade quando utilizado por crianças e por mulheres grávidas ou lactantes. Atualmente, grande parte dos estudos sobre novas substâncias repelentes envolvem óleos essenciais de plantas, principalmente por serem atóxicos, biodegradáveis, possuírem um preço mais acessível e uma ampla atividade contra diferentes espécies de mosquitos. Os terpenos 1,8-cineol, β-cariofileno e α-humuleno podem ser encontrados em óleos essenciais de uma grande variedade de plantas e apesar de dados da literatura mostrarem uma eficácia dessas substâncias como repelentes para outras espécies de insetos, até o presente momento não existem estudos que relacionam porcentagem de repelência ao longo do tempo para avaliar o potencial de repelência das mesmas para culicídeos.O presente estudo avaliou tais substâncias como possíveis repelentes para A. aegypti, utilizando o DEET como controle positivo. Para avaliar a eficácia do β-cariofileno foi utilizada a BG-cage, uma gaiola desenvolvida especificamente para testes de repelência onde o voluntário expõe um braço sob uma abertura coberta por uma tela onde os mosquitos pousam, mas não picam. Os testes foram repetidos a cada 30 minutos após a aplicação do produto por um tempo máximo de duas horas (seguindo a recomendação de reaplicação do produto comercial ...

Monitoramento de cultivos submersos de linhagens de Streptomyces clavuligerus para a quantificação de distribuição de fluxos metabólicos

Pós-graduação em Biotecnologia - IQ