Determination by MAD-DM of the structure of the DNA duplex d[ACGTACG(5-BrU)]2 at 1.46 Å and 100 K

A four-wavelength MAD experiment on a new brominated octanucleotide is reported here. d[ACGTACG(5-BrU)], C77H81BrN30O32P7, (DNA) = 2235, tetragonal, P43212 (No. 96), a = 43.597, c = 26.268 Å, V = 49927.5 Å3, Z = 8, T = 100 K, R = 10.91% for 4312 reflections between 15.0 and 1.46 Å resolution. The self-complementary brominated octanucleotide d[ACGTACG(5-BrU)]2 has been crystallized and data measured to 1.45 Å at both 293 K and a second crystal flash frozen at 100 K. The latter data collection was carried out to the same resolution at the four wavelengths 0.9344, 0.9216, 0.9208 and 0.9003 Å, around the Br K edge at 0.92 Å and the structure determined from a map derived from a MAD data analysis using pseudo-MIR methodology, as implemented in the program MLPHARE. This is one of the first successful MAD phasing experiments carried out at Sincrotrone Elettra in Trieste, Italy. The structure was refined using the data measured at 0.9003 Å, anisotropic temperature factors and the restrained least-squares refinement implemented in the program SHELX96, and the helical parameters are compared with those previously determined for the isomorphous d(ACGTACGT)2 analogue. The asymmetric unit consists of a single strand of octamer with 96 water molecules. No countercations were located. The A-DNA helix geometry obtained has been analysed using the CURVES program.

Pico e flutuação da pressão intra-ocular: comparação entre curva tensional diária e teste de sobrecarga hídrica e comparação entre 2 testes de sobrecarga hídrica em horários diferentes

Pós-graduação em Bases Gerais da Cirurgia - FMB

ECLAC Notes Nº 46

Regional Energy Integration Looks Distant But Could be Crucial WTO and Free Trade Agreement Dispute Settlement Mechanisms Progress OPINION Central America: Poor Terms for the Terms of Trade, by José Luis Machinea HIGHLIGHTS Integration Schemes in Crisis and the Convergence of Trade Agreements, by Osvaldo Rosales INDICATORS: Economic Growth and Employment Direct Action in Favour of Indigenous Peoples and Afro-descendents Rises Recent Titles Calendar

Notas de la CEPAL Nº 46

* Integración energética regional se ve lejana pero podría ser decisiva  * Avances logran mecanismos de solución de controversias en OMC y en acuerdos de libre comercio  * OPINIÓN de José Luis Machinea. Centroamérica: En malos términos con los términos de intercambio  * PRECISIONES de Osvaldo Rosales. Crisis de la integración y convergencia de acuerdos comerciales  * INDICADORES Crecimiento económico y empleo  * Aumentan acciones directas en favor de pueblos indígenas y afrodescendientes  * Publicaciones recientes  * Calendario de eventos