Production and characterization of cellulase-free xylanase from Trichoderma inhamatum

The production of extracellular cellulase-free xylanase from Trichoderma inhamatum was evaluated in liquid Vogel medium with different carbon sources as natural substrates and agricultural or agro-industrial wastes. Optimal production of 244.02 U/mL was obtained with xylan as carbon source, pH 6.0 at 25 degrees C, 120 rpm, and 60-h time culture. Optimal conditions for enzyme activity were 50 degrees C and pH 5.5. Thermal stability of T. inhamatum xylanolytic complex expressed as T(1/2) was 2.2 h at 40 degrees C and 2 min at 50 degrees C. The pH stability was high from 4.0 to 11.0.These results indicate possible employment of such enzymatic complex in some industrial processes which require activity in acid pH, wide-ranging pH stability, and cellulase activity absence.

Production and purification of an Endo–1,4-beta-Xylanase from Humicola grisea var. thermoidea by electroelution

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Water content and GA(3)-induced embryonic cell expansion explain Euterpe edulis seed germination, rather than seed reserve mobilisation

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Effect of fertilization in water quality and in zooplankton community in open plankton-culture ponds

The effect of swine manure fertilization on the water quality of zooplankton artificial culture in two ponds was measured in diel cycles on different months (October 1999, January and March 2000). Fertilization affected directly water quality; values for total phosphorus were above 1.4 mgL(-1) and maximum rates for ammonia reached 108 mu gL(-1). Zooplankton community comprised four species, namely, Brachionus calyciflorus, B. falcatus, Moina sp. and Thermocyclops sp. B. falcatus (Rotifera) and Moina sp (Cladocera) were dominant respectively in January and in March. There was no difference in abundance of zooplankton between the two ponds (p>0.05), although adult cyclopoid Thermocyclops sp was different between diel cycles (p<0.05). There were significant differences (p<0.05) in dissolved oxygen, ammonia, total phosphorus, orthophosphate, nitrite and nitrate between ponds. In fact, only dissolved oxygen and pH were significantly different (p<0.05) between the ponds' surface and bottom. Organic fertilization also provided greater amount of nutrients and higher pH and conductivity, coupled to greater oscillations in the concentration of dissolved oxygen, directly affecting the zooplanktonic composition.

Production and characterization of glucoamylase from fungus Aspergillus awamori expressed in yeast Saccharomyces cerevisiae using different carbon sources

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Production and characterization of a milk-clotting protease in the crude enzymatic extract from the newly isolated Thermomucor indicae-seudaticae N31 (Milk-clotting protease from the newly isolated Thermomucor indicae-seudaticae N31)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Production and characterization of polygalacturonase from thermophilic Thermoascus aurantiacus on submerged fermentation

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

The effect of sodium chloride on the formation of W/O microemulsions in soy bean oil/surfactant/water systems and the solubilization of small hydrophilic molecules

Partial pseudoternary phase diagrams were constructed for soy bean oil (SBO)/surfactant/NaCl aqueous solution systems, at 25 degrees C, using the anionic sodium bis(2-ethylhexyl) sulfosuccinate (ACT) and zwiterionic phosphatidylcholine (PC) or mixtures of these surfactants. The isotropic single phase of water-in-oil (W/O) microemulsions (MEs) is shown in the phase diagram and their viscosity reported. ME samples containing small amount of surfactant exhibit slightly higher viscosity than pure SBO, and were used in the solubilization of small water soluble molecules. NaCl enhances the area of the ME phase and MEs with different surfactant composition exhibit different induction time as obtained from tests of oxidative stability, and so are the MEs enriched with ascorbic acid, folic acid and FeSO4, with the latter exhibiting lower stability. The so prepared enriched soy bean oil has potential application in food industry since the surfactants are food grade. (C) 2007 Elsevier B.V. All rights reserved.

Production and partial characterization of polygalacturonases produced by thermophilic Monascus sp N8 and by thermotolerant Aspergillus sp N12 on solid-state fermentation

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Z gamma production and limits on anomalous ZZ gamma and Z gamma gamma couplings in pp collisions at root s=1.96 TeV

We present a study of eey and mu mu gamma events using 1109 (1009) pb-(1) of data in the electron (muon) channel, respectively. These data were collected with the DO detector at the Fermilab Tevatron pp collider at Is = 1.96 TeV. Having observed 453 (515) candidates in the eey (jtAy) final state, we measure the Z gamma production cross section for a photon with transverse energy ET > 7 GeV, separation between the photon and leptons Delta Rey > 0.7, and invariant mass of the di-lepton pair Mee > 30 GeV/(2)(c), to be 4.96 0.30(stat. + syst.) zE 0.30(lumi.) pb, in agreement with the Standard Model prediction of 4.74 0.22 pb. This is the most precise Zy cross section measurement at a hadron collider. We set limits on anomalous trilinear Zyy and ZZy gauge boson couplings of -0.085 < h(30)(y) < 0.084, -0.0053 < h(40)(y) < 0.0054 and -0.083 < h(30)(Z) < 0.082, 30 40 30 -0.0053 < h(40)(Z) < 0.0054 at the 95% C.L. for the form-factor scale A = 1.2 TeV. 40 Published by Elsevier B.V.

Measurement of W+W- production and search for the Higgs boson in pp collisions at root s=7 TeV

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

First study of the radiation-amplitude zero in W gamma production and limits on anomalous WW gamma couplings at root s=1.96 TeV

We present results from a study of p (p) over bar -> W gamma+X events utilizing data corresponding to 0.7 fb(-1) of integrated luminosity at root s = 1.96 TeV collected by the D0 detector at the Fermilab Tevatron Collider. We set limits on anomalous WW gamma couplings at the 95% C.L. The one-dimensional 95% C.L. limits are 0.49 and -0.12 and the photon and find it to be indicative of the standard model radiation-amplitude zero in the W gamma system.

W gamma Production and Limits on Anomalous WW gamma Couplings in p(p)over-bar Collisions at root s=1.96 TeV

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Z(gamma) production and limits on anomalous ZZ(gamma) and Z(gamma gamma) couplings in p(p)over-bar collisions at root s 1.96 TeV

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)