Metodologia microbiológica convencional e reação em cadeia pela polimerase (PCR) na detecção de Salmonellasp. a partir de caldos de enriquecimento – Tetrathionato e Rappaport-Vassiliadis

The progressive increase in consumption and production of poultry meat in later years comes forth with an increase of the occurrence of foodborne diseases, including salmonellosis. Salmonellosis is caused by the ingestion of contaminated products, mainly by the consumption of poultry meat which processing and preparation for consumption were not effective to eliminate pathogens. Thus, there is a need for the development of faster more sensitive methods of detection of pathogens as a way to ensure the quality of the food offered to consumers. The goal of this essay was to evaluate the effect of enrichment broths on naturally contaminated poultry meat samples. A total of 65 samples was collected, these samples were rinsed with 370 mL of buffered peptone water (BPS) 1% according with the traditional methodology. All of the samples were enriched with both Tetrathionate (TT) and Rappaport-Vassiliadis (RV) and all were analyzed by the convencional identification method and polimerasis chain reaction (PCR). Of the 65 analized samples, 34 (52%) were positive when analized by the conventional method, while 45 (69%) were positive when analized by the PCR. Amongst the 45 positive PCR samples, 44 samples were positive when enriched with TT, while just 32 samples were positive when enriched by RV. Of the 34 positive conventional samples, 29 samples were positive when enriched by TT and 31 were positive when enriched by RV

Proposta de intervenção e gerenciamento das áreas potencialmente contaminadas: estudo dos brownfields no distrito Belém em São Paulo

In the district of Belém, at São Paulo’s city, the economic transformations have ended up destabilizing the economy industrial leaving marks that can be found today. Idle sheds, abandoned or undefined uses structures are found throughout the neighborhood, creating a scene of decay and abandonment. Despite the problems caused by brownfields, they may be seen as an area of possibilities. The reuse of these areas allows the reutilization of abandoned space in locations where the infrastructure is already available, stopping the expansion of urban spot in places not yet degraded. In the case of disabled older industries, there is a great possibility of site’s contamination that may also pose risks to people and unforeseen during refuncionalization. Therefore, the proper management of these sites is needed before any intervention in the area, where stages of study and research must be conducted in order to prove their actual situation. The survey and study of these areas in the district of Belém in São Paulo provides an overview about the brownfields in Brazil at the same time that contributes to the development of intervention plans and proposes a scenery for the refuncionalization of these sites in the neighborhood.

Relações públicas e reputação online: gerenciamento de crise no site americanas.com

The growing need for organizations to make themselves present on the Internet, the study aimed to demonstrate the importance of public relations activity in crisis management and analysis of the crisis in Americanas.com site. It also aims to analyze the electronic scene, highlighting their implications for consumer relations in e-commerce. In addition, as a consequence of conflicts, settles a loss situation, financial and reputation. A crisis begins with planning and communication failures, it is clear that public relations is essential in business today, as interest in integrating their knowledge and opinions, and maintaining a global view of the environment in which it operates. The case study analysis provided confirmation that the public relations face a growing market, which requires qualified and updated to do crisis management, especially in the online environment

Programa para gerenciamento de rebanho leiteiro

With the advent of technology, many repetitive processes before now was be simplified with the support of a simple computer. Therefore programming has been developed and new softwares appear every day. For the development of this project was used the Microsoft Visual Studio 2010, and with it the program was made in C # Windows Form mode. This work aims to develop a program that can read, send and receive data via the Universal Serial Bus (USB) to the computer, and thereby perform the processing. Thus the milk producer will possess the knowledge of the amount of milk produced by the animal. With the processing of data it is possible to generate graphs of herd production or a specific animal and you can also generate the required report date

Análise do Mapa da Cadeia de Valor em um Hospital do Vale do Paraíba

The Lean Thinking is a method used by various industries in order to eliminate waste and ensure quality for the customer. In the current scenario, Lean has been used in other areas such as the hospital in which many tools are being adopted in order to improve its internal processes. One such tool is the Value Stream Mapping, which proved to be an effective tool in industrial environments, and is being adopted by several hospitals. Thus, this paper presents a case study on the use of this tool in a sector of a hospital in the Vale do Paraíba, evaluating its importance, its impacts, positive and negative points and future projections. This is in the context of Lean Healthcare, a new model based on the principles of Lean Thinking which has been adopted by several hospitals and clinics throughout Brazil successfully

Análise do gerenciamento de projetos de produtos na área de desenvolvimento de motores em uma indústria automotiva

L'un des facteurs nécessaires pour qu’une entreprise reste compétitive sur le marché est la création ou l'amélioration d'un produit afin qu’il réponde aux besoins et aux attentes des clients, ainsi, le Processus de Développement de Produits (PDP) est essentiel. Pour la réussite du PDP, il est souhaitable de suivre un système standard de gestion de projet. L'objectif de cette étude est d'analyser la gestion de projets dans le processus de développement de produits dans l'industrie automobile afin d'identifier les possibilités d’amélioration. Par conséquent, nous avons procédé à une revue de littérature sur le sujet, analysé les lignes directrices et les pratiques du processus de développement de produit dans l’entreprise. Comme résultat, il a été obtenu que le système de développement de produits créé par l’entreprise requiert la clarification des outils utiles à la gestion de projet. Un problème de communication et de gestion des coûts a également été constaté dans le domaine du développement du moteur lourd

Freqüência do parvovírus suíno em fetos mumificados, abortados e natimortos detectado através da reação em cadeia pela polimerase (PCR)

Porcine parvovirus (PPV) is associated with reproductive failure and it has been found worldwide, including Brazil. Many diagnostic procedures are used for its detection, for example, immunofluorescence, HA, HI and PCR and this is an important technique because it is very specific and sensitive. In this work, the presence of PPV in fetuses from swine farms with reproductive problems was detected by PCR. All of 170 samples from aborted fetuses, mummies or stillborns were sampled by PCR with primers designed to VP2 region of PPV and c-myc (endogenous control). Only 142 samples (83,53%) were positive for c-myc and among them six samples (4,22%) were positive for PPV which were tested in HA. In this test, erythrocytes suspension 1% was used and three samples (50%) agglutinated but they presented low titer (4). For this work, porcine parvovirus was detected in the samples analyzed by PCR and HA. It is important to emphasize the use of endogenous control when material with elevated degree of autolysis is examined

Dimensão ética do gerenciamento em enfermagem

In managing an Inpatient unit, nurses face the need to make decisions involving agents in the institution’s internal and external environments, and all these measures are influential, regardless of how beneficial they may be. Hence, ethical issues constitute an important dimension of nursing management. This study aimed at identifying measures and/or interventions adopted by managing nurses at Inpatient Units in the Botucatu School of Medicine University Hospital - UNESP with the purpose to analyze professional ethics. The study involved nurses holding technicalsupervision positions in sectors related to the Nursing Division, comprising a total number of 20. The exploratory method was used by means of taped interviews with a qualitative approach based on the Collective Subject Discourse. Human resources deficit was observed as nurses’ major concern in relation to nursing care provision. The managers seek the nursing staff’s development by means of educational activities in their own unit at the same time that they adopt those offered by the hospital. When ethical violations occur, they resort to measures ranging from individual strategies for violators’ orientation to legal intervention. The resolution of ethical conflicts by nursing managers generally occurs in a gradual fashion by taking into account their compromising level, whether in relation to the patient or to the multiprofessional team. Educational activities are focused on the development of technical skills aiming at preventing physical damage to patients although educational activities targeted at the development of professionals’ ethical awareness were not mentioned

Sistema de Gerenciamento de Clínica - “CEAFIR”

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Avaliação do gerenciamento e disposição final dos resíduos químicos de laboratórios do IGCE, UNESP - Rio Claro/SP

Laboratory of Chemical Waste Management programs are being implemented in various universities of the country and the world, in recognition of the urgent need to change the reality of disregard for the environment, together with objective responsibility of the generator, and especially the awareness of sustainability. Analysis and research laboratories involve a range of waste in developing their studies with intrinsic characteristics related to their form of generation. The amount of waste generated in this segment is negligible compared to industrial activities, but the environmental issue is that these residues do not have a standard technique for treatment due to the potential variation of its composition. This research project, aiming their suitability and continuous improvement, aims to diagnose and analyze the current situation of the management and disposal of chemical waste generated by the laboratories IGCE UNESP, Campus Rio Claro / SP. Universities, through their research, teaching and extension, end up generating potentially hazardous chemicals that may contaminate the environment when they are disposed of improperly, with no concern for the environment and / or public health. Aiming to assist in improvements with respect to this issue, the purpose of this study is to understand the management and disposal of chemical waste from the IGCE, laboratories UNESP - Rio Claro / SP, in order to discuss the situation of such waste, and present proposals to reduce generation of the same and minimizing the environmental impact, thereby increasing the local hygiene through proper disposal. The research instrument used interviews, questionnaires, review of recent literature and observations were made in order to develop proposals for the management and disposal of waste. These proposals based on prevention and corrective control, where the preventive approach aims at lifting techniques and actions to reduce the generating sources and...

Gerenciamento dos resíduos de serviços de saúde do pronto socorro de um hospital escola na cidade de Botucatu

Health Care Waste (HCW) represents 1%, and it has presently gained a lot of importance. Adequate management is one of the great challenges to be faced by health care centers. It has gained distinction and been widely discussed by members of the sectors involved with sanitation, public health and environmental issues due to waste physical, chemical biological characteristics, which pose potential risk to the environment and public health. The present study aims at evaluating HCW internal management by following all its phases, determining indicators, classifying and quantifying, establishing production rates (kg /patient/day) for the sector and designing materials to disseminate appropriate HCW disposal in the Emergency Room of the UNESP University Hospital in the city of Botucatu according to the guidelines presently in force. : From June to October 2011, the waste flow was observed from its production to final disposal. Four weight measurements were performed on four consecutive days in the month of August by using a properly calibrated (in grams) digital scale at the times scheduled for collection of the produced waste. Hence, the daily and monthly amounts were estimated according to their classification. All the waste packaged in the bags in garbage cans in the Emergency Room for a 24-hour period was considered to be a sample. Separation was not adequately performed in that sector, and waste from Group A was mixed with that from Group D. The amount of infectious waste produced in the sector corresponded to 87.80 %, common waste to 10.93 % and recyclable waste to 1.27%. The mean daily HCW production was of 123.300 kg/day, and the total monthly production was of 3,822 kg/month, which was distributed as follows: Group A 3,355,750 kg/month; Group D 417,570 kg/month and recyclables 48,670 kg/month. The production rate corresponded to 0.47 kg/patient/day, thus showing... (Complete abstract click electronic access below)

Zoneamento acústico urbano e mapeamento de níveis sonoros na zona central de Rio Claro (SP): diretrizes para o gerenciamento do ruído integrado ao planejamento ambiental urbano

Considering the growing degradation of the urban environmental quality resulting from the progressive sound pollution increase, as well as the consequent harmful effects about the human health, the present study had its central objective the argument and management tools implementation of the urban noise that can be integrated to the environmental planning in the city of Rio Claro (SP). Therefore, normative and bibliographical revisions were developed, that made feasible the directives of control argument and prevention of the urban noise, as well as the technical procedures of sound levels and evaluation’s definition and especially about the criteria applicable for the elaboration of an urban acoustic zoning methodology. Among the main results obtained detach: (1) the applicable legislation to the management of urban noise’s synthesis and argument; (2) the methodology of urban acoustic zoning development and (3) its implementation to determined areas of Rio Claro (SP) with the (4) respective cartography representation; (5) the mapping of sound levels in the central zone of Rio Claro (SP), (6) the obtaining and analysis of noise describers (LAeq, Lmáx, L10, L50, L90, Lmin and TNI) and the subsequent (7) acoustic maps elaboration for the main describers.

Abordagem scrum de gerenciamento ágil para desenvolvimento de novos produtos aplicada a área de ensaios de uma fabricante de aeronaves

There are many approaches and techniques about Administration and Management of Projects in order to provide greater agility, efficiency and transparency during the development process of new products. Simultaneously, a pursuit for a management approach more flexible in its planning and strategic changes during the development campaign, such as, comprehend the project's unpredictability level and deal it by monitoring and estimates tools. These features tend to accentuate itself in manufacturers of complex products, as aircraft and other aerospace technologies. By these conditions thus research aims to describe a case when the Agile Management Processing of Project Development Scrum was used in the Test area of an aircraft manufacturer. Focused on the Scrum implementation over the area, its adaptation, evolution and achievements the research proposes to analyze the improvements, indicate the obstacles and discuss solutions, contributing then to the theoretical basis of the considered theme and futures updates applicable to the area. The research is classified as qualitative; furthermore the information and data analyzed were obtained by interviews with professionals and observations of the processes from the major aircraft manufacturer

Metodologia Microbiológica Convencional e Reação em Cadeia pela Polimerase (PCR) na detecção de Salmonella spp em carcaças de frango

Salmonella is the etiological agent responsible for one of the most important Food Borne Disease (FBD), Salmonellosis, which generates significant economic consequences in several countries, including Brazil. Poultry meat is one of the most important disseminators of the pathogen. Accordingly, several countries have developed programs trying to reduce the prevalence of Salmonella in poultry meat. Such programs are based on the research of the pathogen in the carcasses, establishing a maximum limit of positive samples at each set of analysis. The Salmonella scans are usually made using the conventional microbiological methods, which tend to be expensive and time consuming. In recent years were developed rapid methods such as polymerase chain reaction (PCR), which can greatly shorten the results time, showing greater sensitivity and specificity than conventional methodology

PGCP - Plataforma para Gerenciamento Colaborativo de Projetos

Collaborative Systems are designed to support individuals in order to solve a problem together, or cooperatively (or collaboratively). Such systems have the advantage of not requiring the presence in the same location of the participants. The basis of any Collaborative System is communication, where users interact with each other in order to exchange information. Communication among individuals is accomplished through collaborative tools such as chat, email, document repository, etc.. Using these tools, companies can reduce operational costs, saving on travel and accommodation of its employees to attend meetings. The project members only need a computer with internet access to use collaborative tools. Project management is essential to the quality of the nal product. To perform a proper management, methodologies must be applied using methods, tools and procedures. There are some phases that are considered generic for all software process models, they are: de nition, development, maintenance and activity to support the software process. In this paper is described a platform for project management using the concepts and features of Collaborative Systems. The goal of this platform is to unify various collaborative tools (such as chat and document repository) in a project management system. Using the system, it becomes possible to manage projects, and the ability to maintain an active constant communication between members of each project