Holocene fossil woods from the Caldera de Taburiente National Park (Canary Islands, Spain)

The study of 39 Pinus canariensis Holocene fossil woods from the Caldera de Taburiente is presented

Flood Hazard Analysis in the ”Caldera de Taburiente” National Park (La Palma, Canary Islands, Spain) using Dendrogeomorphology

Aplicación de técnicas dendroecológicas para el estudio de avenidas torrenciales.

Contexto, criptoanálisis y propuesta de solución de la inscripción de la talla (original) de la Virgen de Candelaria de Tenerife (Canarias, España)

Esta investigación es un ejemplo de simbiosis entre criptoanálisis y desciframiento de lenguas. Es la búsqueda del sentido de una inscripción, un conjunto de casi doscientas letras latinas, en una talla de la Virgen María que estaba en la isla de Tenerife, en la localidad hoy de Candelaria, en las islas Canarias. La imagen desapareció en un temporal en el año 1826. No obstante, es posible lograr una gran certeza sobre qué letras tenía, acudiendo a las fuentes documentales textuales y artísticas. El conocimiento del significado, si lo hubiera, de la inscripción mariana, creemos que no puede lograrse sin la adecuada comprensión del contexto. Esto significa indagar en la historia de la misma talla, que se remonta hasta el siglo XIV o XV, en el estudio de la población autóctona canaria, así como de los pueblos que allí llegaron en sus diferentes momentos históricos. Además, es necesario conocer el redescubrimiento del archipiélago canario y sus procesos de conquista y evangelización. Todos estos datos irán ofreciendo un panorama nuevo y sorprendente para comprender no sólo las letras sino la misma imagen escultórica en madera. A partir de este momento la indagación se moverá en ver si las letras corresponden a alguna lengua posible, lo que nos ha llevado a analizar un amplísimo conjunto de textos lo más cercanos a la época bajo estudio, pertenecientes a alrededor de un centenar de lenguas. Tras el examen lingüístico se ha procedido a un estudio de las posibles formas criptográficas que se hubieran utilizado para generar el texto de la inscripción. Se ofrece un detallado y minucioso elenco de técnicas posibles que pudieran haberse adoptado y se criptoanaliza con exhaustividad las letras de la talla mariana. Al mismo tiempo se ofrece un nuevo marco criptológico de métodos y sistemas más ordenado y completo que el que hasta ahora venía considerándose, en especial desde el surgimiento de la criptografía de clave asimétrica. Continuamos la investigación sopesando la posible generación pseudo-aleatoria del texto, un texto que pudiera no tener sentido alguno. En este momento, y habiendo completado todas las posibilidades e hipótesis, habiéndose negado todas, volvemos a reconsiderar el cuerpo de conjeturas y supuestos. Desde ahí analizamos en profundidad el ámbito etnográfico y lingüístico bereber como hipótesis más plausible y probable. Tras la profundización en esta lengua y la corrección de los errores que nos llevaron a no detectarla en nuestro análisis precedente, llegamos a la conclusión de encontrarnos ante una lengua arcaica bereber, un conjunto de letras pertenecientes a una lengua y familia hoy no desaparecida, si bien muy modelada y difuminada por otras lenguas, en especial el árabe. Esto nos llevará a rescatar aspectos léxicos, morfológicos, sintácticos y fonéticos de este habla arcaica. Con todos estos datos realizamos un amplio estudio semántico de la talla tanto desde la perspectiva aborigen autóctona como cristiana. Finalmente, desde las voces lexicales y sus raíces de las lenguas bereberes e insulares amazigh, ofrecemos el significado de las letras inscritas en la talla mariana de Candelaria. ABSTRACT This research is an example of symbiosis between cryptanalysis and deciphering of languages. It is the search for meaning in an inscription, a group of about two hundred latin letters on a carving of the Virgin Mary that was on the island of Tenerife, in the town of Candelaria today, in the Canary islands. The image disappeared in a storm in 1826. However, it is possible to achieve a great certainty about what letters had, going to the textual and artistic documentary sources. The knowledge of the meaning, if any, of the marian inscription, can not be achieved without an adequate knowledge of the context. This means researching into the history of the same carving, which dates back to the fourteenth and fifteen century; the study of the canarian indigenous people and of the people who came there at different historical moments. Furthermore, it is necessary to know the rediscovery of the Canary islands and their processes of conquest and evangelization. All these data will offer a new and surprising outlook to understanding not only the letters but the same wood sculpture. From this moment the inquiry will move to see if the letters correspond to any possible language, which has led us to analyze a very large set of texts as close to the time under study, in a hundred languages. After the language examination, has been carried out a study of possible cryptographic forms used to generate the text of the inscription. A detailed and thorough list of possible techniques that could be adopted is offered. Then exhaustively we cryptanalyze the letters of the marian carving. At the same time a new crypto framework of methods and systems more orderly and complete, especially since the emergence of asymmetric key cryptography, is provided. We continue researching the possible pseudo-random generation of the text, a text that would not make any sense. At this time, and having completed all the possibilities and hypotheses, all having refused, we return to rethink our assumptions. From there we analyze in depth the ethnographic and linguistic berber sphere as the most likely hypothesis. Following the deepening of this language and correcting the mistakes that led us not to detect it in our analysis above, we conclude that this is an archaic berber language, a set of letters belonging to a language and family not extinct today but very modeled and influenced by other languages, primarily arabic. This will lead us to rescue lexical, morphological, syntactic and phonetic aspects of this archaic speech. With all this data we make a wide semantic study of the carving from the indigenous and christian perspective. Finally, from the lexical voices and roots of the berber languages amazigh and island-amazigh, we give the meaning of the letters inscribed in the marian carving of Candelaria.

Effect of the season on the free phytoprostanes content in Cornicabra extra virgin olive oil from deficit irrigated olive trees

BACKGROUND: The effect of regulated deficit irrigation (RDI) on the phytoprostane (PhytoP) content in extra virgin olive (Olea europaea L., cv. Cornicabra) oil (EVOO) was studied. During the 2012 and 2013 seasons, T0 plants were irrigated at 100% ETc, while T1 and T2 plants were irrigated avoiding water deficit during phases I and III of fruit growth and saving water during the non-critical phenological period of pit hardening (phase II), developing amore severewater deficit in T2 plants. In 2013, a fourth treatment (T3) was also performed, which was similar to T2 except that water saving was from the beginning of phase II to 15 days after the end of phase II. RESULTS: 9-F1t-PhytoP, 9-epi-9-F1t-PhytoP, 9-epi-9-D1t-PhytoP, 9-D1t-PhytoP, 16-B1-PhytoP and 9-L1-PhytoP were present in Cornicabra EVOO, and their contents increased in the EVOO fromRDI plants. CONCLUSION: Deficit irrigation during pit hardening or for a further period of 2 weeks thereafter to increase irrigation water saving is clearly critical for EVOO composition because of the enhancement of free PhytoPs, which have potential beneficial effects on human health. The response of individual free PhytoPs to changes in plant water status was not as perceptible as expected, preventing their use as biomarkers of water stress.

Fauna hawaiiensis; being the land-fauna of the Hawaiian islands. by various authors, 1899-1913.

v. 1:pt. 6

Fauna hawaiiensis; being the land-fauna of the Hawaiian islands. by various authors, 1899-1913.

v. 1:pt. 2

Fauna hawaiiensis; being the land-fauna of the Hawaiian islands. by various authors, 1899-1913.

v. 1:pt. 3

Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands.

Precise mapping of DNA methylation patterns in CpG islands has become essential for understanding diverse biological processes such as the regulation of imprinted genes, X chromosome inactivation, and tumor suppressor gene silencing in human cancer. We describe a new method, MSP (methylation-specific PCR), which can rapidly assess the methylation status of virtually any group of CpG sites within a CpG island, independent of the use of methylation-sensitive restriction enzymes. This assay entails initial modification of DNA by sodium bisulfite, converting all unmethylated, but not methylated, cytosines to uracil, and subsequent amplification with primers specific for methylated versus unmethylated DNA. MSP requires only small quantities of DNA, is sensitive to 0.1% methylated alleles of a given CpG island locus, and can be performed on DNA extracted from paraffin-embedded samples. MSP eliminates the false positive results inherent to previous PCR-based approaches which relied on differential restriction enzyme cleavage to distinguish methylated from unmethylated DNA. In this study, we demonstrate the use of MSP to identify promoter region hypermethylation changes associated with transcriptional inactivation in four important tumor suppressor genes (p16, p15, E-cadherin, and von Hippel-Lindau) in human cancer.

A common origin for woody Sonchus and five related genera in the Macaronesian islands: molecular evidence for extensive radiation.

Woody Sonchus and five related genera (Babcockia, Taeckholmia, Sventenia, Lactucosonchus, and Prenanthes) of the Macaronesian islands have been regarded as an outstanding example of adaptive radiation in angiosperms. Internal transcribed spacer region of the nuclear rDNA (ITS) sequences were used to demonstrate that, despite the extensive morphological and ecological diversity of the plants, the entire alliance in insular Macaronesia has a common origin. The sequence data place Lactucosonchus as sister group to the remainder of the alliance and also indicate that four related genera are in turn sister groups to subg. Dendrosonchus and Taeckholmia. This implies that the woody members of Sonchus were derived from an ancestor similar to allied genera now present on the Canary Islands. It is also evident that the alliance probably occurred in the Canary Islands during the late Miocene or early Pliocene. A rapid radiation of major lineages in the alliance is consistent with an unresolved polytomy near the base and low ITS sequence divergence. Increase of woodiness is concordant with other insular endemics and refutes the relictural nature of woody Sonchus in the Macaronesian islands.

Preferential isolation of DNA fragments associated with CpG islands.

We describe a procedure for preferential isolation of DNA fragments with G+C-rich portions. Such fragments occur in known genes within or adjacent to CpG islands. Since about 56% of human genes are associated with CpG islands, isolation of these fragments permits detection and probing of many genes within much larger segments of DNA, such as cosmids or yeast artificial chromosomes, which have not been sequenced. Cloned DNA fragments digested with four restriction endonucleases were subjected to denaturing gradient gel electrophoresis. Long G+C-rich sections in fragments inhibit strand dissociation after the fragments reach retardation level in the gradient; such fragments are retained in the gel after most others disappear. Nucleotide sequences of the retained fragments show that about half of these fragments appear to be derived from CpG islands. Northern analysis indicated the presence of RNA complementary to most of the retained fragments. A heuristic approach to the relation between base sequence and the kinetics of strand dissociation of partly melted molecules appears to account for retention and nonretention. The expectation that CpG island fragments will be enriched among fragments retained in a denaturing gradient is supported by rate estimates based on melting theory applied to known sequences. This method, designated SPM for segregation of partly melted molecules, is expected to provide a means for convenient and efficient isolation of genes from unsequenced DNA.

Extraordinarily high spider densities on islands: flow of energy from the marine to terrestrial food webs and the absence of predation.

Some islands in the Gulf of California support very high densities of spiders. Spider density is negatively correlated with island size; many small islands support 50-200 spiders per m3 of cactus. Energy for these spiders comes primarily from the ocean and not from in situ productivity by land plants. We explicitly connect the marine and terrestrial systems to show that insular food webs represent one endpoint of the marine web. We describe two conduits for marine energy entering these islands: shore drift and seabird colonies. Both conduits are related to island area, having a much stronger effect on smaller islands. This asymmetric effect helps to explain the exceptionally high spider densities on small islands. Although productivity sets the maximal potential densities, predation (by scorpions) limits realized spider abundance. Thus, prey availability and predation act in concert to set insular spider abundance.