La narrativa como clave para la investigación enfermera y los cuidados profesionales

En esta comunicación se presenta una narrativa dialógica, a modo de resultados y análisis, con la pretensión de entretejer un discurso científico que permita un diagnóstico y planificación de cuidados profesionales con una perspectiva sistémica, acorde al paradigma de la transformación y al siglo XXI. Las historias que cuentan mujeres y hombres en el contexto profesional según (Guerreiro, 2008) son el retrato dialogado de sus experiencias, a la vez que nos dice que el diagnóstico de enfermería es la narrativa construida a partir de los recortes de estas historias. Por su parte (Kérouac, 2007) nos dice que el paradigma de la transformación representa un cambio de mentalidad sin precedentes. Entiende cada fenómeno como único, es la base de una apertura de la ciencia enfermera hacia el mundo y ha inspirado las nuevas concepciones de la disciplina. El modelo de enfermería de Mishel, (Bailey & Stewart, 2005) cuyo concepto clave es la incertidumbre ante la enfermedad y que la función de la enfermera es valorar el grado de ésta para proporcionar unos cuidados profesionales que contribuyan a una adaptación harmónica, a una nueva perspectiva de vida y a un pensamiento probabilístico, conceptos que se desarrollaran en el transcurso de la comunicación

Fysioterapeuttien kliininen päättely lapsen motorisen kehityksen tukemisessa

kliininen päättely kuuluu olennaisena osana fysioterapeutin työhön. Kliinisessä päättelyssä fysioterapeutti kerää ja prosessoi sekä olemassa olevaa että uutta tietoa ja käyttää sitä asiakkaan terveydentilan, elämäntilanteen ja kuntoutuksen ennusteen arviointiin. Lisäksi fysioterapeutti suunnittelee kerätyn tiedon avulla asiakkaalle yksilöllisesti soveltuvan terapian. Australialainen Ian Edwards on tutkimuksessaan todennut, että fysioterapeutit käyttävät seuraavia kliinisen päättelyn strategioita: diagnostinen päätöksenteko, kertomuksiin liittyvä päätöksenteko, menettelytapoihin liittyvä strategia, vuorovaikutuksellinen päätöksenteko, yhteistoiminnallinen päätöksenteko, eettinen päätöksenteko, ennusteisiin liittyvä päätöksenteko ja päätöksenteko opettamisessa. Istuallaan liikkuminen eli ”peppukiitäminen” voi olla eräs havaittu motorisen kehityksen poikkeavuus noin 1-vuotiaalla lapsella. Lapsi ei ala kontata eikä nousta seisomaan vaan liikkuu mielenkiintoisten kohteiden luokse istuallaan. Tällöin lapsen motorista kehitystä seurataan ja tuetaan esimerkiksi fysioterapeutin vastaanotolla. Opinnäytetyömme tarkoituksena on perehtyä fysioterapeutin kliiniseen päättelyyn terapiatilanteessa, jossa lapsi liikkuu istuallaan. Tarkoituksenamme on myös selvittää, tarvitsevatko istuallaan liikkuvat lapset fysioterapiaa, ja mihin fysioterapeutit perustavat terapian. Olemme haastatelleet kolmea kokenutta, lasten fysioterapian parissa työskentelevää fysioterapeuttia kyseisistä aiheista. Haastattelemamme fysioterapeutit käyttivät kaikkia kliinisen päättelyn strategioita istuallaan liikkuvan lapsen fysioterapiassa. Eniten fysioterapeuteilla oli käytössä diagnostinen sekä menettelytapoihin liittyvä päätöksenteko. Fysioterapeutit vertasivat lapsen kehitystasoa normaaliin motoriseen kehitykseen. Lapsen terapia toteutettiin leikkien, lelujen, laulujen ja lorujen avulla. Vanhempien ohjaaminen koettiin tärkeäksi. Fysioterapeuttien toiminnan perusta oli teoria (esimerkiksi lapsen motorinen kehitys), jonka jälkeen he löysivät pitkästä kokemuksestaan keinot terapian toteuttamiseen. Fysioterapeutit kokivat erilaiset koulutukset sekä moniammatillisen yhteistyön hyödylliseksi. Emme saaneet yksiselitteistä vastausta kysymykseen, tarvitsevatko istuallaan liikkuvat lapset fysioterapiaa. Vanhempien ohjaus näytti haastattelujen perusteella toteutuvan asiantuntijajohtoisesti.

Mesure de l'impact de l'implication des proches aidants de patients hospitalises dans une unité de soins psychiatriques aigus sur eux-mêmes

Le but de cette étude est de mesurer ainsi que de qualifier l'impact de l'implication des proches aidants de patients hospitalisés dans des unités de soins psychiatriques aigus sur eux-mêmes. Le cadre conceptuel utilisé est celui du fardeau des familles de Schene (1990). Il différencie les parties objectives et subjectives du fardeau familial. La récolte de données a été réalisée à l'aide de l'Involvement Evaluation Questionnaire (IEQ). Les résultats de cette étude montrent que les proches aidants souffrent d'un niveau d'inquiétude élevé, 3.8 sur une échelle de Likert à 5 questions, ainsi que d'un niveau de tension à 2.44 sur une échelle de Likert à 5 questions. Des associations ont été trouvées. L'augmentation de la durée du trouble diminue l'inquiétude, avec une valeur ρ de 0.048. Le fait d'être habitué à la situation a également un impact en diminuant la tension, avec une valeur ρ de 0.002. Plus on est « habitué à la situation », avec une valeur ρ de -0.021, moins le proche est inquiet par rapport à la situation du patient. Ainsi que, plus le patient est jeune, plus le proche aidant ressent de tension, avec une valeur ρ de 0.008. Ces résultats, peu généralisables au vu du petit échantillon (n=24), pourraient toutefois impliquer une réflexion approfondie sur l'accueil, la place et le soutien des proches aidants de patients souffrants de troubles psychiatriques hospitalisés dans une unité de soins aigus de la part des infirmières.

A minimal set of coordinates for describing humanoid shoulder motion

The kinematics of the anatomical shoulder are analysed and modelled as a parallel mechanism similar to a Stewart platform. A new method is proposed to describe the shoulder kinematics with minimal coordinates and solve the indeterminacy. The minimal coordinates are defined from bony landmarks and the scapulothoracic kinematic constraints. Independent from one another, they uniquely characterise the shoulder motion. A humanoid mechanism is then proposed with identical kinematic properties. It is then shown how minimal coordinates can be obtained for this mechanism and how the coordinates simplify both the motion-planning task and trajectory-tracking control. Lastly, the coordinates are also shown to have an application in the field of biomechanics where they can be used to model the scapulohumeral rhythm.

Direct Interrogation of Viral Peptides Presented by the Class I HLA of HIV-Infected T Cells

Identification of CD8+ cytotoxic T lymphocyte (CTL) epitopes has traditionally relied upon testing of overlapping peptide libraries for their reactivity with T cells in vitro. Here, we pursued deep ligand sequencing (DLS) as an alternative method of directly identifying those ligands that are epitopes presented to CTLs by the class I human leukocyte antigens (HLA) of infected cells. Soluble class I HLA-A*11:01 (sHLA) was gathered from HIV-1 NL4-3-infected human CD4+ SUP-T1 cells. HLA-A*11:01 harvested from infected cells was immunoaffinity purified and acid boiled to release heavy and light chains from peptide ligands that were then recovered by size-exclusion filtration. The ligands were first fractionated by high-pH high-pressure liquid chromatography and then subjected to separation by nano-liquid chromatography (nano-LC)–mass spectrometry (MS) at low pH. Approximately 10 million ions were selected for sequencing by tandem mass spectrometry (MS/MS). HLA-A*11:01 ligand sequences were determined with PEAKS software and confirmed by comparison to spectra generated from synthetic peptides. DLS identified 42 viral ligands presented by HLA-A*11:01, and 37 of these were previously undetected. These data demonstrate that (i) HIV-1 Gag and Nef are extensively sampled, (ii) ligand length variants are prevalent, particularly within Gag and Nef hot spots where ligand sequences overlap, (iii) noncanonical ligands are T cell reactive, and (iv) HIV-1 ligands are derived from de novo synthesis rather than endocytic sampling. Next-generation immunotherapies must factor these nascent HIV-1 ligand length variants and the finding that CTL-reactive epitopes may be absent during infection of CD4+ T cells into strategies designed to enhance T cell immunity.

Little evidence for association between the TGFBR1*6A variant and colorectal cancer: a family-based association study on non-syndromic family members from Australia and Spain.

Genome-wide linkage studies have identified the 9q22 chromosomal region as linked with colorectal cancer (CRC) predisposition. A candidate gene in this region is transforming growth factor beta receptor 1 (TGFBR1). Investigation of TGFBR1 has focused on the common genetic variant rs11466445, a short exonic deletion of nine base pairs which results in truncation of a stretch of nine alanine residues to six alanine residues in the gene product. While the six alanine (*6A) allele has been reported to be associated with increased risk of CRC in some population based study groups this association remains the subject of robust debate. To date, reports have been limited to population-based case-control association studies, or case-control studies of CRC families selecting one affected individual per family. No study has yet taken advantage of all the genetic information provided by multiplex CRC families. Methods: We have tested for an association between rs11466445 and risk of CRC using several family-based statistical tests in a new study group comprising members of non-syndromic high risk CRC families sourced from three familial cancer centres, two in Australia and one in Spain. Results: We report a finding of a nominally significant result using the pedigree-based association test approach (PBAT; p = 0.028), while other family-based tests were non-significant, but with a p-value < 0.10 in each instance. These other tests included the Generalised Disequilibrium Test (GDT; p = 0.085), parent of origin GDT Generalised Disequilibrium Test (GDT-PO; p = 0.081) and empirical Family-Based Association Test (FBAT; p = 0.096, additive model). Related-person case-control testing using the 'More Powerful' Quasi-Likelihood Score Test did not provide any evidence for association (M-QL5; p = 0.41). Conclusions: After conservatively taking into account considerations for multiple hypothesis testing, we find little evidence for an association between the TGFBR1*6A allele and CRC risk in these families. The weak support for an increase in risk in CRC predisposed families is in agreement with recent meta-analyses of case-control studies, which estimate only a modest increase in sporadic CRC risk among 6*A allele carriers.

Geographic patterns of genetic variation in a broadly distributed marine vertebrate: new insights into loggerhead turtle stock structure from expanded mitochondrial DNA sequences

Previous genetic studies have demonstrated that natal homing shapes the stock structure of marine turtle nesting populations. However, widespread sharing of common haplotypes based on short segments of the mitochondrial control region often limits resolution of the demographic connectivity of populations. Recent studies employing longer control region sequences to resolve haplotype sharing have focused on regional assessments of genetic structure and phylogeography. Here we synthesize available control region sequences for loggerhead turtles from the Mediterranean Sea, Atlantic, and western Indian Ocean basins. These data represent six of the nine globally significant regional management units (RMUs) for the species and include novel sequence data from Brazil, Cape Verde, South Africa and Oman. Genetic tests of differentiation among 42 rookeries represented by short sequences (380 bp haplotypes from 3,486 samples) and 40 rookeries represented by long sequences (~800 bp haplotypes from 3,434 samples) supported the distinction of the six RMUs analyzed as well as recognition of at least 18 demographically independent management units (MUs) with respect to female natal homing. A total of 59 haplotypes were resolved. These haplotypes belonged to two highly divergent global lineages, with haplogroup I represented primarily by CC-A1, CC-A4, and CC-A11 variants and haplogroup II represented by CC-A2 and derived variants. Geographic distribution patterns of haplogroup II haplotypes and the nested position of CC-A11.6 from Oman among the Atlantic haplotypes invoke recent colonization of the Indian Ocean from the Atlantic for both global lineages. The haplotypes we confirmed for western Indian Ocean RMUs allow reinterpretation of previous mixed stock analysis and further suggest that contemporary migratory connectivity between the Indian and Atlantic Oceans occurs on a broader scale than previously hypothesized. This study represents a valuable model for conducting comprehensive international cooperative data management and research in marine ecology.

Pregabalin in the treatment of inferior alveolar nerve paraesthesia following overfilling of endodontic sealer

A case of orofacial pain and inferior alveolar nerve (IAN) paraesthesia after extrusion of endodontic sealer within the mandibular canal treated with prednisone and pregabalin is described. A 36-year-old woman underwent root canal treatment of the mandibular second right premolar tooth. Post-operative panoramic radiograph revealed the presence of radiopaque canal sealer in the mandibular canal. Damage to IAN consecutive to extrusion of endodontic sealer was diagnosed. Non-surgical management was decided, including: 1 mg/kg/day prednisone 2 times/day, once-daily regimen, and 150 mg/day pregabalin, two doses per day, monitoring the progress with periodic follow-up visits. Six weeks after the incident the signs and symptoms were gone. The complete resolution of paraesthesia and the control of pain achieved suggest that a non-surgical approach, combining prednisone and the GABA analogue pregabalin, is a good option in the management of the IAN damage subsequent to endodontic sealer extrusion

Integration of Real-Time Simulator, Motion Platform and Haptics

This thesis describes the process of the integration of a real-time simulator environment with a motion platform and a haptic device as a part of the Kvalive project. Several programs running on two computers were made to control the different devices of the environment. User tests were made to obtain information of needed improvements to make the simulator more realistic. Also new ideas for improving the simulator and directions of further research were obtained with the help of this research.

Aspectos químicos, biológicos e etnofarmacológicos do gênero Cassia

Species of Cassia are widely distributed in tropical and subtropical regions throughout the world, and have been extensively investigated chemically and pharmacologically.They are known to be a rich source of phenolic derivatives, most of them with important biological and pharmacological properties. Some Asian, African and Indian tribes use these species as a laxative, purgative, antimicrobial, antipyretic, antiviral and anti-inflammatory agent. Among a number of other classes of secondary metabolites, such as anthracene derivatives, antraquinones, steroids and stilbenoids, biologically active piperidine alkaloids are an especially important bioactive class of compounds that showed to be restricted to a small group of Cassia species. In this paper we present an overview of the chemical, biological and ethnopharmacological data on Cassia piblished in the literature.

Ratsastussimulaattorin jatkokehitys

Työssä tehtiin jatkokehitystyötä jo olemassa olevaan ratsastussimulaattoriin. Kehitystyö aloitettiin selvittämällä ratsastuksen liiketila, mittaamalla ratsastukseen aikana ratsastajaan kohdistuvat dynaamiset suureet 6 vapausasteen suhteen. Mitatut signaalit käsiteltiin transformaation ja suodatuksen avulla. Käsiteltyjä signaaleja käytettiin simulaattorin liikealustan asemaohjesignaaleina. Liikealustaan päälle kiinnitettyyn hevosenrunkoon muodostettiin antureiden avulla interaktiivinen rajapinta, joka mahdollisti simulaattorin ohjastamisen pohje- ja ohja-avuin. Jatkokehitetty simulaattori toistaa käynti-, ravi- ja laukka-askellajit tunnistettavasti. Simulaattori kuvaa ratsastuksen nopeuden muutosta vaihtamalla askellajia ja muuntamalla portaattomasti askelluksen taajuutta sekä amplitudia.

Constituintes químicos das flores de Pterogyne nitens (Caesalpinioideae)

The phytochemical investigation of the flowers of Pterogyne nitens (Caesalpinioideae) resulted in the isolation and identification of nine phenolic derivatives, quercetin 3-O-sophoroside, taxifolin, astilbin, ourateacatechin, caffeic, ferulic, sinapic, chlorogenic and gallic acid, besides two guanidine alkaloids, pterogynine, pterogynidine. This is the first time these compounds have been reported in P. nitens flowers. As this is a monospecific genus, these secondary metabolites may have taxonomical significance. Their structures were assigned on the basis of spectroscopic analyses, including two-dimensional NMR techniques.

Saponinas antifúngicas de Swartzia langsdorffii

Chromatographic fractionation of the EtOH extract from the leaves of Swartzia langsdorffii afforded the pentacyclic triterpenes oleanolic acid and lupeol, and two saponins: oleanolic acid 3-sophoroside and the new ester 3-O-b-D-(6'-methyl)-glucopyranosyl-28-O-b-D-glucopyranosyl-oleanate. Their structures were elucidated from spectral data, including 2D NMR and HRESIMS experiments. Antifungal activity of all isolated compounds was evaluated, using phytopathogens Cladosporium cladosporioides and C. sphaerospermum, and human pathogens Candida albicans, C. krusei, C. parapsilosis and Cryptococcus neoformans.